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51.发明授权Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy 有权线粒体DNA快速法医分析方法及线粒体DNA异质性表征公开(公告)号：US08802372B2
公开(公告)日：2014-08-12
申请号：US11929930
申请日：2007-10-30
申请人： David J. Ecker , Richard H. Griffey , Rangarajan Sampath , Steven A. Hofstadler , John McNeil , Stanley T. Crooke , Lawrence Blyn , Thomas A. Hall , Yun Jiang , James C. Hannis , Neill White , Vivek Samant , Mark W. Eshoo , Jared James Drader
发明人： David J. Ecker , Richard H. Griffey , Rangarajan Sampath , Steven A. Hofstadler , John McNeil , Stanley T. Crooke , Lawrence Blyn , Thomas A. Hall , Yun Jiang , James C. Hannis , Neill White , Vivek Samant , Mark W. Eshoo , Jared James Drader
CPC分类号： C12Q1/686 , C12Q1/6806 , C12Q1/6844 , C12Q1/6858 , C12Q1/6872 , C12Q1/6888 , C12Q1/689 , C12Q2600/156 , G01N2333/28 , G01N2333/32 , G01N2333/35 , G01N2333/36 , C12Q2563/167 , C12Q2545/101 , C12Q2531/113 , C12Q2565/627 , C12Q2545/113
摘要： The present invention provides methods for rapid forensic analysis of mitochondrial DNA and methods for characterizing heteroplasmy of mitochondrial DNA, which can be used to assess the progression of mitochondrial diseases.
摘要翻译： 本发明提供线粒体DNA的快速法医分析方法和线粒体DNA异质性表征的方法，可用于评估线粒体疾病的进展。
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52.发明申请Method for Rapid Detection and Identification of Bioagents in Epidemiological and Forensic Investigations 有权在流行病学和法医学调查中快速检测和鉴定生物标本的方法公开(公告)号：US20120171679A1
公开(公告)日：2012-07-05
申请号：US11930741
申请日：2007-10-31
申请人： David J. Ecker , Richard H. Griffey , Rangarajan Sampath , Steven A. Hofstadler , John McNeil , Stanley T. Crooke , James C. Hannis
发明人： David J. Ecker , Richard H. Griffey , Rangarajan Sampath , Steven A. Hofstadler , John McNeil , Stanley T. Crooke , James C. Hannis
CPC分类号： C12Q1/689 , C12Q1/6813 , C12Q1/6858 , C12Q1/686 , C12Q1/6888 , C12Q2600/156 , Y02A50/59 , Y02A50/60 , Y02A90/24 , C12Q2565/627 , C12Q2545/113 , C12Q2563/167
摘要： The present invention provides methods for rapid forensic investigations by identification of bioagents associated with biowarfare and acts of terrorism or crime. The methods are also useful for epidemiological investigations by genotyping of bioagents.
摘要翻译： 本发明提供了通过识别与生物武器相关的生物标签和恐怖主义或犯罪行为进行快速法医调查的方法。 该方法也可用于通过基因分型生物学的流行病学调查。
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53.发明授权Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture 有权用于通过溶液捕获的质谱法快速纯化核酸以进行后续分析的方法公开(公告)号：US08158354B2
公开(公告)日：2012-04-17
申请号：US10943344
申请日：2004-09-17
CPC分类号： C12Q1/6806 , C12N15/101 , C12N15/1013 , H01J49/165 , C12Q2563/155 , C12Q2563/143 , C12Q2565/627
摘要： The present invention provides a method for rapid solution capture purification of nucleic acids for subsequent analysis by electrospray mass spectrometry which is efficient and cost-effective relative to existing methods. The present invention also provides kits useful for practicing rapid solution capture of nucleic acids so that purified samples are in condition for analysis by electrospray mass spectrometry.
摘要翻译： 本发明提供了一种用于快速溶液捕获纯化核酸的方法，用于随后通过电喷雾质谱法进行分析，其相对于现有方法是有效且成本有效的。 本发明还提供了用于实施核酸的快速溶液捕获的试剂盒，使得纯化的样品处于通过电喷雾质谱分析的条件下。
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公开(公告)号：US08017743B2
公开(公告)日：2011-09-13
申请号：US11929707
申请日：2007-10-30
IPC分类号： C07H21/02
CPC分类号： C12Q1/689 , C12Q1/686 , C12Q2600/156 , C12Q2565/627
摘要： Method for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a “base composition signature” (BCS) which is then matched against a database of base composition signatures, by which the bioagent is identified.
摘要翻译： 通过核酸扩增和分子量测定的组合来检测和识别未知生物标签的方法，所述引物与衍生自生物制剂的核酸的保守序列区域杂交的引物以及独特的括号可变序列区域 识别生物制剂。 结果是“基本组成签名”（BCS），然后与基础组合签名的数据库进行匹配，通过该数据库识别生物标签。
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公开(公告)号：US08017322B2
公开(公告)日：2011-09-13
申请号：US11233630
申请日：2005-09-21
CPC分类号： C12Q1/689 , C12Q1/686 , C12Q2600/156 , C12Q2565/627
摘要： Method for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a “base composition signature” (BCS) which is then matched against a database of base composition signatures, by which the bioagent is identified.
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公开(公告)号：US20080138808A1
公开(公告)日：2008-06-12
申请号：US11754163
申请日：2007-05-25
CPC分类号： C12Q1/689 , C12Q1/686 , C12Q1/6883 , C12Q2600/16 , Y02A50/57
摘要： The present invention provides compositions, kits and methods for rapid identification and quantification of sepsis-causing bacteria by molecular mass and base composition analysis.
摘要翻译： 本发明提供了通过分子质量和基础组成分析快速鉴定和定量败血症细菌的组合物，试剂盒和方法。
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公开(公告)号：US06730485B2
公开(公告)日：2004-05-04
申请号：US10210684
申请日：2002-07-31
IPC分类号： C12Q168
CPC分类号： G01N33/6842 , C12Q1/6811 , G01N33/566 , G01N33/6848 , G01N2500/02
摘要： Methods of identifying ligand binding sites in a target molecule such as a polynucleotide or polypeptide, as well as methods for determining whether a particular site in a target molecule is at or near the ligand binding site, are provided. Ligand binding affinities corresponding to both the target molecule and a modified version thereof are compared, preferably using mass spectral analysis. The modified molecules, or test molecules, incorporate a modification capable of modulating ligand binding affinity when located at or near the ligand binding sites. Binding site information is derived from the known position of the modification.
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公开(公告)号：US06730484B2
公开(公告)日：2004-05-04
申请号：US10210650
申请日：2002-07-31
IPC分类号： C12Q168
CPC分类号： G01N33/6842 , C12Q1/6811 , G01N33/566 , G01N33/6848 , G01N2500/02
摘要： Methods of identifying ligand binding sites in a target molecule such as a polynucleotide or polypeptide, as well as methods for determining whether a particular site in a target molecule is at or near the ligand binding site, are provided. Ligand binding affinities corresponding to both the target molecule and a modified version thereof are compared, preferably using mass spectral analysis. The modified molecules, or test molecules, incorporate a modification capable of modulating ligand binding affinity when located at or near the ligand binding sites. Binding site information is derived from the known position of the modification.
摘要翻译： 确定靶分子如多核苷酸或多肽中的配体结合位点的方法以及用于确定靶分子中特定位点是否在配体结合位点处或附近的方法。 比较对应于靶分子及其修饰形式的配体结合亲和力，优选使用质谱分析。 当位于配体结合位点处或附近时，修饰的分子或测试分子掺入能够调节配体结合亲和力的修饰。 绑定站点信息来自修改的已知位置。
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公开(公告)号：US09393564B2
公开(公告)日：2016-07-19
申请号：US13259350
申请日：2010-03-30
申请人： David J. Ecker , Steven A. Hofstadler , Rangarajan Sampath , Lawrence B. Blyn , Thomas A. Hall , Mark W. Eshoo
发明人： David J. Ecker , Steven A. Hofstadler , Rangarajan Sampath , Lawrence B. Blyn , Thomas A. Hall , Mark W. Eshoo
CPC分类号： B01L3/502761 , B01L3/5027 , B01L3/52 , B01L7/52 , B01L2200/028 , B01L2200/10 , B01L2300/027 , B01L2400/0415 , H01J49/0022
摘要： The present invention relates to portable systems and devices, and corresponding methods, for detecting bioagents. In particular, the present invention provides systems, devices, and methods that utilize one or more of a sample preparation component, sample analysis component employing broad range primers, and sample detection component.
摘要翻译： 本发明涉及用于检测生物代理的便携式系统和装置及相应的方法。 特别地，本发明提供利用样品制备组分，采用宽范围引物的样品分析组分和样品检测组分中的一种或多种的系统，装置和方法。
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公开(公告)号：US08551738B2
公开(公告)日：2013-10-08
申请号：US12616422
申请日：2009-11-11
CPC分类号： C12Q1/6872 , C12Q1/6827 , C12Q1/6844 , C12Q1/6858 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2565/627 , C12Q2537/143 , C12Q2531/113 , C12Q2545/10
摘要： There is a need for nucleic acid analysis which is both specific and rapid, and in which no nucleic acid sequencing is required. The present invention addresses this need, among others by providing a method of nucleic acid amplification of overlapping sub-segments of a nucleic acid followed by molecular mass measurement of resulting amplification products by mass spectrometry, and determination of the base compositions of the amplification products.
摘要翻译： 需要具有特异性和快速性的核酸分析，并且其中不需要核酸测序。 本发明除了提供核酸扩增核酸扩增方法之外，还通过质谱法对所得扩增产物进行分子量测定，以及确定扩增产物的碱基组成来解决这一需要。
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