公开(公告)号：US07670831B2

公开(公告)日：2010-03-02

申请号：US10860544

申请日：2004-06-03

申请人： Sang Yup Lee ,  Hee Tae Jung ,  Dae Hwan Jung ,  Young Koan Ko ,  Do Hyun Kim ,  Seok Jae Lee ,  Byung Hun Kim ,  Jae Shin Lee

发明人： Sang Yup Lee ,  Hee Tae Jung ,  Dae Hwan Jung ,  Young Koan Ko ,  Do Hyun Kim ,  Seok Jae Lee ,  Byung Hun Kim ,  Jae Shin Lee

IPC分类号： C12M1/00 ,  C12Q1/00 ,  C12Q1/68 ,  C12M1/34

CPC分类号： G01N33/5438 ,  B82Y30/00 ,  C12Q1/001 ,  G01N33/551 ,  Y10S977/742 ,  Y10S977/745 ,  Y10S977/746 ,  Y10S977/747 ,  Y10S977/748 ,  Y10T156/10

摘要： Conductive carbon nanotubes (CNTs) obtained by dotting carboxylated CNTs with metal nanocrystals by chemical functional groups, are described, as well as a method for fabricating a pattern or film of the conductive CNTs which involves repeatedly depositing conductive CNTs on a substrate to achieve high surface density. A biosensor is described, in which bioreceptors that bind to target biomolecules are selectively attached to conductive CNTs or a conductive CNT pattern or film. By use of the conductive biosensor, various target biomaterials that bind or react with the bioreceptors can be precisely measured directly or by electrochemical signals at large amounts in one step. Additionally, the biosensor can be used for an electrical detection method capable of providing precise measurement results even with a small amount of source material.

摘要翻译： 描述了通过化学官能团将金属纳米晶体的羧化CNT点缀的导电碳纳米管（CNT），以及用于制造导电CNT的图案或膜的方法，其包括在基板上反复沉积导电CNT以实现高表面 密度。 描述了生物传感器，其中结合靶生物分子的生物感受器选择性地连接到导电CNT或导电CNT图案或膜。 通过使用导电生物传感器，可以在一个步骤中直接或通过大量的电化学信号精确测量与生物感受器结合或反应的各种靶生物材料。 此外，生物传感器可以用于即使使用少量源材料也能够提供精确测量结果的电检测方法。

公开(公告)号：US07595156B2

公开(公告)日：2009-09-29

申请号：US10819386

申请日：2004-04-06

申请人： Sang Yup Lee ,  Zixin Deng ,  Shi Chen ,  Ki Jun Jeong ,  Xiufen Zhou

发明人： Sang Yup Lee ,  Zixin Deng ,  Shi Chen ,  Ki Jun Jeong ,  Xiufen Zhou

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12P19/62 ,  C07H21/04 ,  C07K14/36 ,  C12N15/52 ,  C12P17/08

摘要： The present invention relates to the base sequence of whole genes involved in the biosynthesis of FR-008 polyketides derived from Streptomyces sp. FR-008. This base sequence comprises genes coding for ketosynthase (KS), acyl transferase (AT), acyl carrier protein (ACP), ketoreductase (KR), dehydratase (DH) and enoyl reductase (ER) domains, and genes coding for modifier enzymes, such as ABC transporter, cytochrome P450 monooxygenase, ferredoxin, thioesterase, sugar synthetic protein, FAD-dependent monooxygenase, 4-amino-4-deoxychorismate (ADC) synthase and ADC lyase. The gene base sequence according to the present invention can be used to increase the productivity of the existing FR-008 polyketides or produce new FR-008 polyketides, through modification of its parts.

摘要翻译： 本发明涉及参与由链霉菌属（Streptomyces sp。）生产的FR-008聚酮化合物的生物合成的全基因的碱基序列。 FR-008。 该碱基序列包括编码酮解酶（KS），酰基转移酶（AT），酰基载体蛋白（ACP），酮还原酶（KR），脱水酶（DH）和烯酰还原酶（ER）结构域的基因，以及编码修饰酶的基因， 作为ABC转运蛋白，细胞色素P450单加氧酶，铁氧还蛋白，硫酯酶，糖合成蛋白，FAD依赖性单加氧酶，4-氨基-4-脱氧激酶（ADC）合酶和ADC裂解酶。 根据本发明的基因碱基序列可用于通过改变其部分来提高现有FR-008聚酮化合物的生产率或生产新的FR-008聚酮化合物。

公开(公告)号：US20090215048A1

公开(公告)日：2009-08-27

申请号：US11994330

申请日：2005-10-14

申请人： Sang Yup Lee ,  Tae Yong Kim ,  Dong Yup Lee

发明人： Sang Yup Lee ,  Tae Yong Kim ,  Dong Yup Lee

IPC分类号： C12Q1/68 ,  G06F19/00 ,  C12N15/00 ,  C12N15/74 ,  C12P7/46

CPC分类号： G16B5/00 ,  G16B20/00

摘要： The present invention relates to an in silico method for improving an organism on the basis of the flux sum (φ) of metabolites, and more particularly to a method for screening key metabolites that increase the production yield of a useful substance, the method comprising defining the metabolite utilization of an organism for producing a useful substance as flux sum and perturbing the flux sum, as well as a method for improving an organism producing a useful substance, the method comprising deleting and/or amplifying genes associated with the aforementioned screened key metabolites. According to the present invention, the correlation between specific metabolites and useful substance production can be exactly predicted, so that it is possible to develop an organism having increased useful substance production by introducing and/or amplifying and/or deleting genes expressing enzymes associated with the specific metabolites. In addition, it is also possible to increase the production of a useful substance by adding specific metabolites during culture.

摘要翻译： 本发明涉及一种基于代谢物的通量和（phi）改善生物体的计算机方法，更具体地涉及一种筛选提高有用物质产量的关键代谢物的方法，该方法包括定义 用于产生有用物质的生物体的代谢物利用作为助熔剂和扰乱通量和，以及改善产生有用物质的生物体的方法，所述方法包括删除和/或扩增与上述筛选的关键代谢物相关的基因 。 根据本发明，可以准确地预测特定代谢物和有用物质生产之间的相关性，从而可以通过引入和/或扩增和/或删除表达与...相关的酶的基因来开发具有增加的有用物质生产的生物体 特异性代谢物。 此外，还可以通过在培养期间加入特定的代谢物来增加有用物质的产生。

公开(公告)号：US20090075352A1

公开(公告)日：2009-03-19

申请号：US11722632

申请日：2005-05-23

申请人： Sang Yup Lee ,  Tae Yong Kim ,  Dong Yup Lee ,  Sang Jun Lee

发明人： Sang Yup Lee ,  Tae Yong Kim ,  Dong Yup Lee ,  Sang Jun Lee

IPC分类号： C12P7/46 ,  G06G7/48 ,  C12N1/21

CPC分类号： C12P7/46 ,  C12N9/1205 ,  C12N15/1089

摘要： The present invention is related to a method for improving a strain on the basis of in silico analysis, in which it compares the genomic information of a target strain for producing a useful substance to the genomic information of a strain overproducing the useful substance so as to primarily screen genes unnecessary for the overproduction of the useful substance, and then to secondarily screen genes to be deleted through performing simulation with metabolic flux analysis. According to the present invention, an improved strain can be effectively constructed by the metabolic and genetic engineering approach comprising comparatively analyzing the genomic information of a target strain for producing a useful substance and the genomic information of a strain producing a large amount of the useful substance to screen candidate genes and performing in silico simulation on the screened candidate genes to select a combination of genes to be deleted, which shows an improvement in the production of the useful substance. Accordingly, the time, effort and cost required for an actual wet test can be significantly reduced.

摘要翻译： 本发明涉及一种基于在计算机分析中改进菌株的方法，其中将用于产生有用物质的靶菌株的基因组信息与过量产生有用物质的菌株的基因组信息进行比较，以便 主要是用于过量生产有用物质的筛选基因，然后通过用代谢通量分析进行模拟来次要筛选待缺失的基因。 根据本发明，可以通过代谢和基因工程方法有效地构建改进的菌株，其包括比较分析用于产生有用物质的靶菌株的基因组信息和产生大量有用物质的菌株的基因组信息 筛选候选基因并在筛选的候选基因的计算机模拟中进行选择要缺失的基因的组合，其显示有用物质的生产的改进。 因此，可以显着降低实际湿测试所需的时间，精力和成本。

公开(公告)号：US07470530B2

公开(公告)日：2008-12-30

申请号：US10580556

申请日：2004-05-20

申请人： Sang Yup Lee ,  Sang Jun Lee

发明人： Sang Yup Lee ,  Sang Jun Lee

IPC分类号： C12N1/20 ,  C12N1/12 ,  C12N9/10 ,  C12P7/46

CPC分类号： C12P7/46 ,  C12N1/20 ,  C12R1/01

摘要： Provided are novel rumen bacterial mutants resulted from the disruption of a lactate dehydrogenase gene (ldhA) and a pyruvate formate-lyase gene (pfl) from rumen bacteria; a novel bacterial mutant (Mannheimia sp. LPK7) having disruptions of a ldhA, a pfl,a phosphotransacetylase gene (pta), and a acetate kinase gene (ackA); a novel bacterial mutant (Mannheimia sp. LPK4) having disruptions of a ldhA, a pfl, and a phosphoenolpyruvate carboxylase gene (ppc) involved in the immobilization of CO2 in a metabolic pathway of producing succinic acid; and a method for producing succinic acid, characterized by culture of the above mutants in anaerobic conditions. The bacterial mutants have the property of producing succinic acid at high concentration while producing little or no organic acids, as compared to the prior wild-type strains of producing various organic acids. Thus, the bacterial mutants are useful as strains for the industrial production of succinic acid.

摘要翻译： 提供的是由瘤胃细菌破坏乳酸脱氢酶基因（ldhA）和丙酮酸甲酸酯裂解酶基因（pfl）而产生的新型瘤胃细菌突变体; 具有破坏ldhA，pfl，磷酸转乙酰酶基因（pta）和乙酸激酶基因（ackA）的新型细菌突变体（Mannheimia sp.LPK7）; 具有涉及将CO 2固定在产生琥珀酸的代谢途径中的ldhA，pfl和磷酸烯醇丙酮酸羧化酶基因（ppc）的破坏的新型细菌突变体（Mannheimia sp.LPK4）; 以及生产琥珀酸的方法，其特征在于在厌氧条件下培养上述突变体。 与生产各种有机酸的现有野生型菌株相比，细菌突变体具有以高浓度生产琥珀酸的能力，同时产生很少或没有有机酸。 因此，细菌突变体可用作工业生产琥珀酸的菌株。

公开(公告)号：US07291325B2

公开(公告)日：2007-11-06

申请号：US10545849

申请日：2003-07-10

申请人： Sang Yup Lee ,  Mee Jung Han ,  Si Jae Park

发明人： Sang Yup Lee ,  Mee Jung Han ,  Si Jae Park

IPC分类号： A61K48/00 ,  A01N63/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12P21/02 ,  C07K14/245 ,  C07K14/5434 ,  C07K14/57 ,  C07K14/65 ,  C12N15/70

摘要： A method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. Two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli are described. The first method enhances the secretory production and activity of target proteins using ibpA and/or ibpB genes-deleted bacteria. The second method enhances the production of target proteins in the cytoplasm and also converts the target proteins from soluble form to insoluble inclusion body, using ibpA and/or ibpB gene-amplified bacteria.

摘要翻译： 通过缺失或扩增编码包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的方法。 描述了使用编码大肠杆菌的包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的两种方法。 第一种方法使用ibpA和/或ibpB基因缺失的细菌增强了靶蛋白的分泌生产和活性。 第二种方法增强细胞质中靶蛋白的产生，并使用ibpA和/或ibpB基因扩增的细菌将靶蛋白从可溶形式转化为不溶性包涵体。

公开(公告)号：US07148334B2

公开(公告)日：2006-12-12

申请号：US10791059

申请日：2004-03-02

申请人： Sang Yup Lee ,  Mee-Jung Han ,  Si Jae Park

发明人： Sang Yup Lee ,  Mee-Jung Han ,  Si Jae Park

IPC分类号： C07K1/26

CPC分类号： C07K14/195 ,  C07K14/00

摘要： The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional (2-D) gel electrophoresis. Furthermore, the present invention relates to the improved method of 2-D gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the 2-D gel electrophoresis, thereby obtaining 2-D gel with much more protein spots.

摘要翻译： 本发明涉及含有用于预防蛋白质降解的sHSP和用于二维（2-D）凝胶电泳的组合物的组合物。 此外，本发明涉及2-D凝胶电泳的改进方法，其特征在于使用sHSP。 根据本发明，在2-D凝胶电泳中防止了蛋白质斑点的减少，从而获得了具有更多蛋白质斑点的2-D凝胶。

公开(公告)号：US06274345B1

公开(公告)日：2001-08-14

申请号：US09507323

申请日：2000-02-18

申请人： Sang Yup Lee ,  Zhaohui Xu ,  Jong Hyun Choi

发明人： Sang Yup Lee ,  Zhaohui Xu ,  Jong Hyun Choi

IPC分类号： C12N1500

CPC分类号： C12N15/70 ,  C07K14/245 ,  C07K2319/00

摘要： The present invention relates to expression vectors containing a gene encoding outer membrane protein C(OmpC) from Escherichia coli as a cell surface anchoring motif, more specifically, to expression vectors comprising a gene encoding OmpC which is designed to express a gene of foreign protein in fused form with OmpC on the cell surface of Escherichia coli, and a method for displaying the desired protein on the surface of the bacteria employing the OmpC as a cell surface anchoring motif. In accordance with the present invention, the desired protein can be expressed efficiently on the cell surface of bacteria so that the expressed protein can be applied to a variety of applications such as live vaccine development, peptide libraries screening, antibody production, environmental bioadsorbent, whole cell catalysis, and biosensor development.

摘要翻译： 本发明涉及包含编码来自大肠杆菌的外膜蛋白C（OmpC）的基因作为细胞表面锚定基序的表达载体，更具体地，涉及包含编码OmpC的基因的表达载体，所述基因编码OmpC，其被设计为表达外源蛋白质的基因 与OmpC融合形成在大肠杆菌的细胞表面上，以及使用OmpC作为细胞表面锚定基序在细菌表面显示所需蛋白质的方法。 根据本发明，所需蛋白质可以在细菌的细胞表面上有效表达，使得表达的蛋白质可以应用于各种应用，例如活疫苗开发，肽文库筛选，抗体生产，环境生物吸附剂，整体 细胞催化和生物传感器开发。

公开(公告)号：US09120891B2

公开(公告)日：2015-09-01

申请号：US13001001

申请日：2009-06-24

申请人： Sang Yup Lee ,  Yu Kyung Jung ,  Taek Ho Yang ,  Si Jae Park ,  Tae Wan Kim

发明人： Sang Yup Lee ,  Yu Kyung Jung ,  Taek Ho Yang ,  Si Jae Park ,  Tae Wan Kim

IPC分类号： C12P7/18 ,  C12P7/42 ,  C12N1/20 ,  C12N15/74 ,  C07H21/04 ,  C08G63/06 ,  C12P7/62 ,  C12P21/02

CPC分类号： C08G63/06 ,  C12P7/625 ,  C12P21/02

摘要： Provided is a method of preparing polylactate (PLA) or a copolymer thereof using a mutant microorganism in which a gene participating in a coenzyme A (CoA) donor- and lactate-producing pathway is genetically manipulated to increase the productivity of a CoA donor and lactate. Amounts of the CoA donor and the lactate are simultaneously increased in a microbial metabolic pathway to enable effective biosynthesis of PLA and a hydroxyalkanoate-lactate copolymer having a high content of lactate, which is industrially useful.

摘要翻译： 提供了使用突变微生物制备聚乳酸（PLA）或其共聚物的方法，其中遗传操作参与辅酶A（CoA）供体和乳酸生产途径的基因以提高CoA供体和乳酸的生产率 。 CoA供体和乳酸盐的量在微生物代谢途径中同时增加，以有效生物合成PLA和具有高含量乳酸盐的羟基链烷酸酯 - 乳酸共聚物，其在工业上是有用的。

公开(公告)号：US09096872B2

公开(公告)日：2015-08-04

申请号：US13497743

申请日：2010-09-21

申请人： Sang Yup Lee ,  Yu-Sin Jang ,  Jin Young Lee ,  Joung Min Lee ,  Jin Hwan Park

发明人： Sang Yup Lee ,  Yu-Sin Jang ,  Jin Young Lee ,  Joung Min Lee ,  Jin Hwan Park

IPC分类号： C12N1/21 ,  C12P7/16 ,  C12N9/04 ,  C12N9/10

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y203/01008 ,  C12Y203/01019 ,  Y02E50/10

摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.

摘要翻译： 本发明涉及生产丁醇的能力提高的重组微生物，以及使用其制备丁醇的方法。 更具体地，本发明涉及通过操作其代谢网络来增加生产丁醇的能力的重组微生物，以及使用其生产丁醇的方法。 在具有编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中，具有增加的产生丁醇能力的重组微生物包括编码转化乙酰CoA至乙酸的酶的基因的缺失。 通过操作微生物的代谢通过获得的重组微生物能够以高效率选择性地生产丁醇，因此可用作生产工业溶剂和运输燃料的微生物。