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126 条记录 (耗时 0.052 秒)

    Design principle for the construction of expression constructs for gene therapy
    52.
    发明申请
    Design principle for the construction of expression constructs for gene therapy 有权
    用于构建基因治疗表达构建体的设计原理

    公开(公告)号：US20030054392A1

    公开(公告)日：2003-03-20

    申请号：US10228811

    申请日：2002-08-27

    申请人： SOFT GENE GMBH

    发明人： Burghardt Wittig ,  Claas Junghans

    IPC分类号： C12Q001/68 ,  C07H021/04

    CPC分类号： C12N15/87 ,  A61K48/00 ,  Y10S977/916

    摘要： The invention concerns an expressible nucleic acid construct, which contains only the sequence information necessary for expressing a gene for RNA or protein synthesis. Expression constructs of this type can be used in gene therapy and genetic vaccination and avoid many of the risks associated with constructs today. The invention further concerns the possibility of improving the conveying of the construct into cells or tissue by covalent linkage of the construct, for example to particles of peptides.

    摘要翻译： 本发明涉及可表达的核酸构建体，其仅含有表达用于RNA或蛋白质合成的基因所必需的序列信息。 这种类型的表达构建体可用于基因治疗和基因疫苗接种，并避免今天与构建相关的许多风险。 本发明还涉及通过构建体的共价连接（例如与肽颗粒）将构建体转运到细胞或组织中的可能性。

    Surgical irrigation solution and method for inhibition of pain and inflammation
    55.
    发明授权
    Surgical irrigation solution and method for inhibition of pain and inflammation 有权
    手术灌注液和抑制疼痛和炎症的方法

    公开(公告)号：US06420432B2

    公开(公告)日：2002-07-16

    申请号：US09837141

    申请日：2001-04-17

    申请人： Gregory A. Demopulos ,  Pamela A. Pierce ,  Jeffrey M. Herz

    发明人： Gregory A. Demopulos ,  Pamela A. Pierce ,  Jeffrey M. Herz

    IPC分类号： A61K31135

    CPC分类号： A61K31/439 ,  A61K31/00 ,  A61K31/352 ,  A61K31/40 ,  A61K31/4045 ,  A61K31/4164 ,  A61K31/4168 ,  A61K31/4174 ,  A61K31/435 ,  A61K31/4406 ,  A61K31/4427 ,  A61K31/4439 ,  A61K31/444 ,  A61K31/48 ,  A61K31/498 ,  A61K31/505 ,  A61K31/506 ,  A61K31/538 ,  A61K45/06 ,  Y10S977/738 ,  Y10S977/904 ,  Y10S977/916 ,  Y10S977/919

    摘要： A method and solution for perioperatively inhibiting a variety of pain and inflammation processes at wounds from general surgical procedures including oral/dental procedures. The solution preferably includes multiple pain and inflammation inhibitory at dilute concentration in a physiologic carrier, such as saline or lactated Ringer's solution. The solution is applied by continuous irrigation of a wound during a surgical procedure for preemptive inhibition of pain and while avoiding undesirable side effects associated with oral, intramuscular, subcutaneous or intravenous application of larger doses of the agents. One preferred solution to inhibit pain and inflammation includes a serotonin2 antagonist, a serotonin3 antagonist, a histamine antagonist, a serotonin agonist, a cyclooxygenase inhibitor, a neurokinin1 antagonist, a neurokinin2 antagonist, a purinoceptor antagonist, an ATP-sensitive potassium channel opener, a calcium channel antagonist, a bradykinin1 antagonist, a bradykinin2 antagonist and a &mgr;-opioid agonist.

    摘要翻译： 一种围手术期抑制来自一般外科手术（包括口腔/牙科手术）的伤口的各种疼痛和炎症过程的方法和解决方案。 该溶液优选包括在生理载体例如盐水或乳酸林格氏溶液中以稀释浓度抑制的多种疼痛和炎症。 在外科手术过程中连续冲洗伤口以解决疼痛的预防性作用，同时避免与口服，肌肉内，皮下或静脉内施用较大剂量的药剂相关的不良副作用。 抑制疼痛和炎症的一个优选的解决方案包括5-羟色胺2拮抗剂，5-羟色胺3拮抗剂，组胺拮抗剂，5-羟色胺激动剂，环加氧酶抑制剂，神经激肽1拮抗剂，神经激肽2拮抗剂，嘌呤受体拮抗剂，ATP-敏感钾通道开放剂， 钙通道拮抗剂，缓激肽1拮抗剂，缓激肽2拮抗剂和μ-阿片样物质激动剂。

    Methods and kits for isolating biological target materials using silica magnetic particles
    56.
    发明申请
    Methods and kits for isolating biological target materials using silica magnetic particles 审中-公开
    使用二氧化硅磁性颗粒分离生物靶材的方法和试剂盒

    公开(公告)号：US20020086326A1

    公开(公告)日：2002-07-04

    申请号：US10041890

    申请日：2002-01-07

    申请人： Promega Corporation

    发明人： Craig E. Smith ,  Charles K. York

    IPC分类号： C12Q001/68 ,  C12N015/00

    CPC分类号： C12N15/1013 ,  C12Q1/6806 ,  Y10S977/838 ,  Y10S977/904 ,  Y10S977/916 ,  Y10S977/92 ,  Y10S977/924 ,  Y10S977/96

    摘要： The present invention provides methods for isolating biological target materials, particularly nucleic acids, such as DNA or RNA or hybrid molecules of DNA and RNA, from other substances in a medium using silica magnetic particles. The methods of the present invention involve forming a complex of the silica magnetic particles and the biological target material in a mixture of the medium and particles, separating the complex from the mixture using external magnetic force, and eluting the biological target material from the complex. The preferred embodiments of magnetic silica particles used in the methods and kits of the present invention are capable of forming a complex with at least 2 nullg of biological target material per milligram of particle, and of releasing at least 60% of the material from the complex in the elution step of the method. The methods of the present invention produce isolated biological target material which is substantially free of contaminants, such as metals or macromolecular substances, which can interfere with further processing or analysis, if present.

    摘要翻译： 本发明提供了使用二氧化硅磁性颗粒从介质中的其他物质分离生物靶材料，特别是DNA或RNA或DNA和RNA的混合分子的方法。 本发明的方法包括在介质和颗粒的混合物中形成二氧化硅磁性颗粒和生物目标材料的复合物，使用外部磁力从混合物中分离复合物，并从复合物中洗脱生物目标物质。 在本发明的方法和试剂盒中使用的磁性二氧化硅颗粒的优选实施方案能够与每毫克颗粒至少2个生物靶材料形成复合物，并且从复合物中释放至少60％的材料 在该方法的洗脱步骤中。 本发明的方法产生基本上不含污染物（例如金属或大分子物质）的分离的生物靶材料，如果存在可能会干扰进一步的加工或分析。

    Sub-100nm biodegradable polymer spheres capable of transporting and releasing nucleic acids
    57.
    发明授权
    Sub-100nm biodegradable polymer spheres capable of transporting and releasing nucleic acids 有权
    亚100nm可生物降解的聚合物球能够运输和释放核酸

    公开(公告)号：US06254890B1

    公开(公告)日：2001-07-03

    申请号：US09209031

    申请日：1998-12-10

    申请人： Sachiko Hirosue ,  Bernhard G. Mueller ,  Robert S. Langer ,  Richard C. Mulligan

    发明人： Sachiko Hirosue ,  Bernhard G. Mueller ,  Robert S. Langer ,  Richard C. Mulligan

    IPC分类号： A61K951

    CPC分类号： A61K9/5153 ,  Y10S977/792 ,  Y10S977/795 ,  Y10S977/915 ,  Y10S977/916

    摘要： The present invention provides biodegradable polymer nanospheres capable of transporting and releasing therapeutic agents, specifically nucleic acids. In preferred embodiments, a sub-150 nm nanosphere is formed containing nucleic acids. Thereafter, the agent is released from the nanosphere. In one embodiment a biodegradable polymer nanosphere surface has attached to it a targeting moiety. In another embodiment, the biodegradable polymer nanosphere surface has attached to it a masking moiety. In yet another embodiment both targeting and masking moieties are attached to the nanosphere surface.

    摘要翻译： 本发明提供了能够运输和释放治疗剂，特别是核酸的可生物降解的聚合物纳米球。 在优选的实施方案中，形成含有核酸的亚150nm纳米球。 此后，试剂从纳米球中释放出来。 在一个实施方案中，可生物降解的聚合物纳米球表面附着有靶向部分。 在另一个实施方案中，可生物降解的聚合物纳米球表面已经附着了掩蔽部分。 在另一个实施方案中，靶向和掩蔽部分均连接到纳米球表面。