公开(公告)号：US12060415B2

公开(公告)日：2024-08-13

申请号：US17592828

申请日：2022-02-04

申请人： Rutgers, The State University of New Jersey

发明人： Renata Pasqualini ,  Wadih Arap ,  Fernanda Iamassaki Staquicini ,  Fortunato Ferrara ,  Sara D'Angelo ,  Andrew R. M. Bradbury

IPC分类号： C07K16/18 ,  A61K39/395 ,  A61P35/00 ,  C07K16/30 ,  G01N33/574 ,  A61K38/07 ,  A61K39/00 ,  A61K47/68 ,  A61K49/00 ,  C07K14/47 ,  C07K16/00 ,  C07K16/28 ,  C12N15/10 ,  C12N15/64 ,  C12Q1/6869 ,  G01N33/50

CPC分类号： C07K16/18 ,  A61K39/3955 ,  A61K39/39558 ,  A61P35/00 ,  C07K16/30 ,  C07K16/3023 ,  G01N33/574 ,  A61K38/07 ,  A61K2039/505 ,  A61K47/6803 ,  A61K47/6817 ,  A61K47/6843 ,  A61K47/6849 ,  A61K47/6891 ,  A61K49/0008 ,  C07K14/47 ,  C07K16/005 ,  C07K16/28 ,  C07K16/2866 ,  C07K2317/21 ,  C07K2317/35 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/77 ,  C12N15/1037 ,  C12N15/64 ,  C12N2810/00 ,  C12Q1/6869 ,  G01N33/5011 ,  G01N33/57423

摘要： Isolated or recombinant EphA5 or GRP78 targeting antibodies are provided. In some cases, antibodies of the embodiments can be used for the detection, diagnosis and/or therapeutic treatment of human diseases, such as cancer. A method of rapidly identifying antibodies or antibody fragments for the treatment of cancer using a combination of in vitro and in vivo methodologies is also provided.

公开(公告)号：US12060412B2

公开(公告)日：2024-08-13

申请号：US16087036

申请日：2016-10-27

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Aviv Regev ,  Feng Zhang ,  Naomi Habib ,  Yinqing Li ,  Matthias Heidenreich ,  Lukasz Swiech ,  Anindita Basu ,  David Weitz ,  Inbal Avraham Davidi

IPC分类号： C07K16/10 ,  C12N15/10 ,  C12Q1/686 ,  C12Q1/6869

CPC分类号： C07K16/1036 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2537/165 ,  C12Q2563/179 ,  C12Q2565/514 ,  C12Q2565/629 ,  C12Q1/6869 ,  C12Q2537/165 ,  C12Q2563/179 ,  C12Q2565/514 ,  C12Q2565/629

摘要： Transcriptomes of individual neurons provide rich information about cell types and dynamic states. However, it is difficult to capture rare dynamic processes, such as adult neurogenesis, because isolation from dense adult tissue is challenging, and markers for each phase are limited. Here, Applicants developed Nuc-seq, Div-Seq, and Dronc-Seq. Div-seq combines Nuc-Seq, a scalable single nucleus RNA-Seq method, with EdU-mediated labeling of proliferating cells. Nuc-Seq can sensitively identify closely related cell types within the adult hippocampus. Div-Seq can track transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead. Finally, Applicants found rare adult newborn GABAergic neurons in the spinal cord, a non-canonical neurogenic region. Taken together, Nuc-Seq, Div-Seq and Dronc-Seq allow for unbiased analysis of any complex tissue.

公开(公告)号：US20240263168A1

公开(公告)日：2024-08-08

申请号：US18636580

申请日：2024-04-16

申请人： Life Technologies Corporation

发明人： Chiu Tai Andrew Wong ,  Mark Beauchemin ,  Shanti Shankar ,  Kylan Szeto

IPC分类号： C12N15/10 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/10

CPC分类号： C12N15/1065 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/1081

摘要： A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.

公开(公告)号：US20240263164A1

公开(公告)日：2024-08-08

申请号：US18610023

申请日：2024-03-19

申请人： Illumina, Inc.

发明人： Scott Kuersten ,  Frederick W. Hyde ,  Asako Tetsubayashi

IPC分类号： C12N15/10 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/6876

CPC分类号： C12N15/1003 ,  C12N15/1024 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/6876

摘要： The present disclosure is related to methods and materials for depleting unwanted RNA species from a nucleic acid sample. In particular, the present disclosure describes how to remove unwanted rRNA, tRNA, mRNA or other RNA species that could interfere with the analysis, manipulation and study of target RNA molecules in a sample.

公开(公告)号：US12054852B2

公开(公告)日：2024-08-06

申请号：US15665226

申请日：2017-07-31

申请人： The Regents of the University of California

发明人： Vivek K. Mutalik ,  Adam Deutschbauer ,  Adam P. Arkin ,  Pavel Novichkov

IPC分类号： C40B30/06 ,  C12N15/10

CPC分类号： C40B30/06 ,  C12N15/1065

摘要： Disclosed herein are barcoded expression libraries comprising a plurality of expression vectors, wherein each expression vector comprises a nucleic acid fragment flanked by a first barcode and a second barcode. Further disclosed herein are methods of making the barcoded expression libraries and methods of conducting functional analysis using the barcoded expression libraries.

公开(公告)号：US12054783B2

公开(公告)日：2024-08-06

申请号：US17210191

申请日：2021-03-23

申请人： GUARDANT HEALTH, INC.

发明人： AmirAli Talasaz

IPC分类号： C12P19/34 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6886 ,  G16B30/00 ,  G16B30/10

CPC分类号： C12Q1/6886 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6874 ,  G16B30/00 ,  G16B30/10 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q1/6869 ,  C12Q2537/165 ,  C12Q2545/114

摘要： The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.

公开(公告)号：US12054755B2

公开(公告)日：2024-08-06

申请号：US17683299

申请日：2022-02-28

申请人： Massachusetts Institute of Technology

发明人： Joseph M. Jacobson ,  Noah Michael Jakimo ,  Pranam Chatterjee

IPC分类号： C12N1/00 ,  C12N9/22 ,  C12N15/09 ,  C12N15/10 ,  C12N15/11 ,  C12N15/66

CPC分类号： C12N9/22 ,  C12N1/00 ,  C12N15/09 ,  C12N15/102 ,  C12N15/11 ,  C12N15/66 ,  C12N2310/20

摘要： A Streptococcus canis Cas9 (ScCas9) ortholog and its engineered variants, possessing novel PAM specificity, is an addition to the family of CRISPR-Cas9 systems. ScCas9 endonuclease is used in complex with guide RNA, consisting of identical non-target-specific sequence to that of the guide RNA SpCas9, for specific recognition and activity on a DNA target immediately upstream of either an “NNGT” or “NNNGT” PAM sequence. A novel DNA-interacting loop domain within ScCas9, and other Cas9 orthologs, such as those from Streptococcus gordonii and Streptococcus angionosis facilitates a divergent PAM sequence from the “NGG” PAM of SpCas9.

公开(公告)号：US12054712B2

公开(公告)日：2024-08-06

申请号：US17022597

申请日：2020-09-16

申请人： ILLUMINA, INC.

发明人： Tingting Jiang

IPC分类号： C12N15/11 ,  A61K35/13 ,  C07K16/28 ,  C12N15/10 ,  C12Q1/6869 ,  C12Q1/6886

CPC分类号： C12N15/11 ,  A61K35/13 ,  C07K16/2818 ,  C07K16/2827 ,  C12N15/1003 ,  C12Q1/6869 ,  C12Q1/6886

摘要： Methods and systems are provided for differentiating between cancer variants and somatic variants originating from hematopoietic cells in a cell free DNA sample. In some embodiments, the cancer variants can be distinguished from somatic variants originating from hematopoietic cells based on fragment size distribution.

公开(公告)号：US12054707B2

公开(公告)日：2024-08-06

申请号：US17227799

申请日：2021-04-12

申请人： New England Biolabs, Inc.

发明人： Zhenyu Zhu ,  Aine Quimby ,  Shuang-Yong Xu ,  Shengxi Guan ,  Hua Wei ,  Penghua Zhang ,  Dapeng Sun ,  Siu-hong Chan

IPC分类号： C12N15/10 ,  C12N9/16 ,  C12N9/22

CPC分类号： C12N15/1058 ,  C12N9/16 ,  C12N9/22 ,  C12N15/1034 ,  C12N15/1086

摘要： Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor.

公开(公告)号：US20240255395A1

公开(公告)日：2024-08-01

申请号：US18565948

申请日：2022-06-03

申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA

发明人： Abdennour Abbas ,  Akli Zarouri

IPC分类号： G01N1/34 ,  C12N15/10

CPC分类号： G01N1/34 ,  C12N15/1017

摘要： A device for separating biological entities from a fluid sample, the device comprising: porous material comprising bound material, wherein the bound material functions to separate the biological entity from the fluid matrix. Devices including such porous media, methods of using such porous media and methods of making such porous media are also included herein.