公开(公告)号：US20140329307A1

公开(公告)日：2014-11-06

申请号：US14290057

申请日：2014-05-29

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Alexander Shenderov ,  Michael G. Pollack

IPC: B01L7/00

CPC classification number: B01L7/525 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/1816 ,  B01L2300/1827 ,  B01L2300/1872 ,  B01L2400/0427

Abstract: Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones.

Abstract translation: 描述了需要多个反应温度的用于进行化学或生化反应的方法和装置。 所述方法包括在微流体装置上移动一个或多个反应液滴或反应体积通过具有不同温度的各种反应区。 这些装置包括微流体装置，其包括能够通过各种反应区移动反应液滴或反应体积的适当致动器。

公开(公告)号：US20140302580A1

公开(公告)日：2014-10-09

申请号：US14308110

申请日：2014-06-18

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: G01N1/38

CPC classification number: G01N33/54326 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0809 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N1/38 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnate field. Other embodiments are also provided.

Abstract translation: 本发明提供了一种在液滴致动器中的液滴内分散或循环磁响应珠的方法。 在一个实施例中，本发明利用具有配置成传送液滴的多个液滴操作电极的液滴致动器和存在于多个液滴操作电极的一部分处的磁场。 在均匀磁场存在的情况下，在液滴致动器上提供含珠的液滴。 在孵育期间，通过在大气场的均匀区域内的液滴上进行液滴操作，珠子在液滴中循环。 还提供了其他实施例。

公开(公告)号：US20140231259A1

公开(公告)日：2014-08-21

申请号：US14253556

申请日：2014-04-15

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Philip Y. Paik ,  Vamsee K. Pamula

IPC: G01N27/447

CPC classification number: B01L3/502792 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L7/54 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/0448 ,  B03C5/02 ,  B81B1/00 ,  G01N27/447 ,  G01N2035/1046

Abstract: The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator including an opaque filler fluid and a transparent droplet thereon is provided. The droplet microactuator may further include a first substrate comprising electrodes configured to effect electrowetting mediated droplet operations on a surface of the first substrate; a second substrate spaced apart from the surface of the first substrate by a distance sufficient to define an interior volume between the first substrate and second substrate; and wherein the droplet and the filler fluid are in the interior volume, and wherein the droplet is surrounded by the filler fluid and arranged with respect to the electrodes in a manner which permits electrowetting mediated droplet operations to be effected on the droplet using the electrodes.

Abstract translation: 本发明涉及用于液滴操作的填充流体。 根据该方面的一个实施例，提供了包括不透明填充流体和其上的透明液滴的液滴微致动器。 液滴微致动器还可以包括第一衬底，其包括被配置为在第一衬底的表面上实现电润湿介导的液滴操作的电极; 第二基板，与第一基板的表面间隔开足以限定第一基板和第二基板之间的内部空间的距离; 并且其中所述液滴和所述填充流体在所述内部容积中，并且其中所述液滴被所述填充流体包围并且以允许使用所述电极对所述液滴进行电润湿介导的液滴操作的方式相对于所述电极布置。

公开(公告)号：US10139403B2

公开(公告)日：2018-11-27

申请号：US15266693

申请日：2016-09-15

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Ramakrishna Sista ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: B01L3/00 ,  G01N1/00 ,  G01N1/10 ,  G01N15/06 ,  G01N1/18 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  G01N1/38 ,  B01F13/08 ,  B01L7/00 ,  G01N35/10

Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.

公开(公告)号：US20160318021A1

公开(公告)日：2016-11-03

申请号：US15206860

申请日：2016-07-11

Applicant: Advanced Liquid Logic Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Philip Paik

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L3/0241 ,  B01L3/50273 ,  B01L2200/06 ,  B01L2300/06 ,  B01L2400/0427 ,  B41J2/14

Abstract: A method of manipulating a droplet comprising providing a substrate comprising a surface; an elongated transport electrode disposed on the substrate surface, the elongated transport electrode having a first and a second end and configured to impart a gradient force to the droplet; and one or more wires for providing power to the transport electrode; and providing power to the one or more wires to effect the gradient force and thereby transport the droplet along the length of the elongated transport electrode from the first end to the second end.

公开(公告)号：US09377455B2

公开(公告)日：2016-06-28

申请号：US14746276

申请日：2015-06-22

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  G01N1/38 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54326 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0809 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N1/38 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.

公开(公告)号：US20160068901A1

公开(公告)日：2016-03-10

申请号：US14888137

申请日：2014-05-01

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Allen E. Eckhardt ,  Michael G. Pollack ,  David S. Cohen

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01L7/52 ,  C12Q1/6844 ,  C40B20/00 ,  C12Q2533/101 ,  C12Q2565/301

Abstract: The invention provides pyrosequencing-based methods of analyzing and synthesizing DNA, including methods of DNA error correction, determining DNA size distribution, screening for nucleotide repeat disorders such as fragile X syndrome, determining size distribution and bias in a DNA library, and determining pyrosequencing read length. The methods include on-bench protocols as well as droplet-based protocols that may be conducted on a droplet actuator.

Abstract translation: 本发明提供了基于焦磷酸测序的DNA分析和合成方法，包括DNA错误校正方法，确定DNA大小分布，筛选核苷酸重复序列如脆性X综合征，确定DNA文库中的大小分布和偏差，以及确定焦磷酸测序读数 长度。 这些方法包括工作台协议以及可以在液滴致动器上进行的基于液滴的协议。

公开(公告)号：US20150174578A1

公开(公告)日：2015-06-25

申请号：US14590470

申请日：2015-01-06

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Zishan Hua ,  Arjun Sudarsan ,  Philip Y. Paik

IPC: B01L3/00

CPC classification number: B01L3/52 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/0241 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/143 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/06 ,  B01L2400/0688 ,  F04B19/006

Abstract: A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration comprises one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.

Abstract translation: 具有与液滴操作表面相关联的液滴形成电极配置的液滴致动器，其中所述电极配置包括一个或多个电极，其被配置为在液滴操作表面上形成子液滴期间控制液滴的体积。 还提供了制造和使用液滴致动器的方法。

公开(公告)号：US20150021189A1

公开(公告)日：2015-01-22

申请号：US14455645

申请日：2014-08-08

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Vamsee K. Pamula ,  Ramakrishna Sista ,  Michael G. Pollack

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L3/502761 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/089 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0448

Abstract: A droplet actuator configured to improve the throughput of droplet operations in a detection spot of the droplet actuator and/or to reduce carryover problems is provided. The droplet actuator may include electrodes configured for effecting droplet operations transporting droplets on a surface; a sensor arranged in proximity to one or more of the electrodes establishing a detection window on the surface for detection of one or more properties of one or more droplets on the surface; wherein the electrodes may establish at least two pathways for transport of droplets into the detection window.

Abstract translation: 提供了一种配置成提高液滴致动器的检测点中的液滴操作的吞吐量和/或减少携带问题的液滴致动器。 液滴致动器可以包括被配置为用于实现在表面上传送液滴的液滴操作的电极; 设置在一个或多个电极附近的传感器，在表面上建立检测窗口，以检测表面上的一个或多个液滴的一个或多个特性; 其中电极可以建立用于将液滴输送到检测窗中的至少两个路径。

公开(公告)号：US20140332380A1

公开(公告)日：2014-11-13

申请号：US14338889

申请日：2014-07-23

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ryan A. Sturmer ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Philip Y. Paik

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0627 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0809 ,  B01L2400/0427 ,  G01N27/22 ,  G01N27/223 ,  G01N27/227 ,  G01N2203/00 ,  G01V3/02

Abstract: A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.

Abstract translation: 一种用于检测液滴致动器上电容的方法，电路和装置，特别是用于通过以下步骤确定液滴致动器上的电极处的液滴的存在，部分存在或不存在：（a）提供液滴致动器，其包括：（i） 基板，其包括布置在所述基板上的电极，用于在所述基板的表面上进行液滴操作; （ii）电容检测电路，用于检测一个或多个电极处的液滴操作表面处的电容; （b）在一个或多个电极处检测液滴操作表面处的电容; 和（c）根据电容确定在电极处的液滴操作表面处的液滴的存在，部分存在或不存在。