公开(公告)号：US5648477A

公开(公告)日：1997-07-15

申请号：US477320

申请日：1995-06-07

申请人： Jan Leemans ,  Johan Botterman ,  Charles Thompson ,  Rao Mouva

发明人： Jan Leemans ,  Johan Botterman ,  Charles Thompson ,  Rao Mouva

IPC分类号： A01H5/00 ,  A01H20060101 ,  A01H1/00 ,  A01H4/00 ,  A01H5/10 ,  A01N63/00 ,  A01N63/02 ,  A46B20060101 ,  C07K14/00 ,  C07K14/195 ,  C07K14/41 ,  C12N20060101 ,  C12N5/00 ,  C12N5/10 ,  C12N9/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/11 ,  C12N15/54 ,  C12N15/63 ,  C12N15/70 ,  C12N15/76 ,  C12N15/82 ,  C12R1/465 ,  C12R1/55

CPC分类号： A01H1/00 ,  C12N15/63 ,  C12N15/70 ,  C12N15/76 ,  C12N15/8277 ,  C12N9/1029 ,  C07K2319/00 ,  Y10S47/01

摘要： The invention relates to a DNA fragment containing a determined gene, the expression of which inhibits the antibiotic and herbicidal effects of Bialaphos and related products.It also relates to recombinant vectors, containing such DNA fragment, which enable this protective gene to be introduced and expressed into cells and plant cells.

摘要翻译： 本发明涉及一种含有确定基因的DNA片段，其表达抑制了Bialaphos及相关产品的抗生素和除草效果。 它还涉及含有这种DNA片段的重组载体，其使得能够将该保护性基因导入细胞和植物细胞中。

公开(公告)号：US5322937A

公开(公告)日：1994-06-21

申请号：US708866

申请日：1991-05-31

申请人： Akira Arisawa ,  Naoto Kawamura ,  Ikuo Kojima ,  Kazuhiko Okamura ,  Hiroshi Tone ,  Rokuro Okamoto

发明人： Akira Arisawa ,  Naoto Kawamura ,  Ikuo Kojima ,  Kazuhiko Okamura ,  Hiroshi Tone ,  Rokuro Okamoto

IPC分类号： C07H17/08 ,  C12N1/21 ,  C12N9/10 ,  C12N15/55 ,  C12N15/76 ,  C12P19/62 ,  C12P21/06 ,  A01N37/18 ,  C07H19/00

CPC分类号： C07H17/08 ,  C12N15/76 ,  C12N9/1029 ,  C12P19/62 ,  Y10S435/822

摘要： A DNA fragment or a restriction enzyme-digested DNA fragment, the DNA fragment containing a gene, acyA, encoding a 3-acylation enzyme for macrolide antibiotics, characterized by being derived from a strain belonging to the genus Streptomyces, having a size of about 1.8 kb or about 3.2 kb, and having a DNA base sequence shown in a restriction enzyme map shown in FIG. 1(A) or FIG. 1(B), respectively, in the attached drawing. 3-Acylated macrolide antibiotics can be produced advantageously using macrolide antibiotics-producing bacteria transformed with vector plasmids having inserted therein the DNA fragment.

摘要翻译： DNA片段或限制酶消化的DNA片段，含有编码大环内酯类抗生素3-酰化酶的基因acyA的DNA片段，其特征在于衍生自属于链霉菌属（Streptomyces）属的菌株，大小约为1.8 kb或约3.2kb，并且具有如图1所示的限制酶图谱所示的DNA碱基序列。 图1（A） 1（B），附图中。 3-酰基化大环内酯类抗生素可以有利地用大环内酯抗生素产生细菌转化，其中插入有DNA片段的载体质粒转化。

公开(公告)号：US5264354A

公开(公告)日：1993-11-23

申请号：US898353

申请日：1992-06-12

申请人： Patricia J. Solenberg

发明人： Patricia J. Solenberg

IPC分类号： C12N1/21 ,  C12N15/00 ,  C12N15/76

CPC分类号： C12N15/76 ,  C12N15/00

摘要： A novel method for isolating transposable elements was used to isolate an approximately 1.6 kb insertion sequence from Streptomyces. The method entails transforming a cell with a plasmid containing a repressor gene, so that the introduction of a transposable element into the gene allows the expression of a selectable marker in a second host cell. The novel insertion sequence isolated from Streptomyces lividans CT2 has been designated IS493.

摘要翻译： 用于分离转座元件的新方法用于从Streptomyces中分离出大约1.6kb的插入序列。 该方法需要用含有阻遏物基因的质粒转化细胞，从而将可转座元件引入基因允许在第二宿主细胞中表达选择性标记。 从链霉菌（Streptomyces lividans）CT2分离的新插入序列已经被命名为IS493。

公开(公告)号：US5252474A

公开(公告)日：1993-10-12

申请号：US490723

申请日：1990-03-14

申请人： Keith M. Gewain ,  Douglas J. MacNeil ,  Tanya MacNeil ,  Philip S. Paress ,  Carolyn L. Ruby ,  Stanley L. Streicher

发明人： Keith M. Gewain ,  Douglas J. MacNeil ,  Tanya MacNeil ,  Philip S. Paress ,  Carolyn L. Ruby ,  Stanley L. Streicher

IPC分类号： C12N9/10 ,  C12N9/88 ,  C12N15/10 ,  C12N15/52 ,  C12N15/76 ,  C12P17/18 ,  C12P19/62 ,  C12N15/00 ,  C12N15/11

CPC分类号： C12P17/181 ,  C12N15/10 ,  C12N15/52 ,  C12N15/76 ,  C12N9/1007 ,  C12N9/88 ,  C12P19/623

摘要： There are disclosed plasmids containing DNA isolated from Streptomyces avermitilis, the microorganism which is used to prepare avermectin compounds, identified as pAT1, pVE650 pVE855, pVE859, pVE1446, pVE923, and pVE924 which contain the genetic information for the biosynthesis of the avermectins. Methods for the isolation of such plasmid and for the manipulation of the plasmids to alter the formation of the avermectin compound are also disclosed.

摘要翻译： 公开了含有从阿维链霉菌（Streptomyces avermitilis）分离的DNA的质粒，该微生物用于制备被鉴定为pAT1，pVE650 pVE855，pVE859，pVE1446，pVE923和pVE924的除虫菌素化合物，其含有阿维菌素生物合成的遗传信息。 还公开了分离这种质粒和用于操纵质粒以改变阿维菌素化合物形成的方法。

公开(公告)号：US5242809A

公开(公告)日：1993-09-07

申请号：US967949

申请日：1992-10-27

申请人： Craig W. Adams ,  Mary E. Brawner ,  James A. Fornwald ,  Francis J. Schmidt

发明人： Craig W. Adams ,  Mary E. Brawner ,  James A. Fornwald ,  Francis J. Schmidt

IPC分类号： C12N15/52 ,  C12N15/76 ,  C12N15/90

CPC分类号： C12N15/90 ,  C12N15/52 ,  C12N15/76

摘要： A recombinant DNA molecule comprising the Streptomyces gal operon galK gene; galE gene; galT gene; P1 promoter, P2 promoter, P2 promoter expression unit; P1 promoter regulated region; or the entire Streptomyces gal operon.

摘要翻译： 包含链霉菌gal操纵子galK基因的重组DNA分子; galE基因; galT基因; P1启动子，P2启动子，P2启动子表达单位; P1启动子调控区; 或整个Streptomyces gal操纵子。

公开(公告)号：US5175105A

公开(公告)日：1992-12-29

申请号：US179345

申请日：1988-04-08

申请人： Bernd Meyhack ,  Jutta Heim ,  Rolf Burgi

发明人： Bernd Meyhack ,  Jutta Heim ,  Rolf Burgi

IPC分类号： A61K38/46 ,  A61P7/02 ,  C07K20060101 ,  C07K1/20 ,  C07K1/22 ,  C07K14/00 ,  C07K14/745 ,  C12N20060101 ,  C12N1/16 ,  C12N1/19 ,  C12N9/16 ,  C12N9/26 ,  C12N9/72 ,  C12N15/00 ,  C12N15/09 ,  C12N15/12 ,  C12N15/58 ,  C12N15/76 ,  C12N15/81 ,  C12P20060101 ,  C12P21/00 ,  C12P21/02 ,  C12R1/865

CPC分类号： C12N9/2408 ,  C12N15/81 ,  C12N9/16 ,  C12N9/6462 ,  C12Y304/21073 ,  C07K2319/02

摘要： Novel human plasminogen activators of the urokinase type are produced by yeast cells transformed with a hybrid vector comprising a DNA sequence coding for said human plasminogen activator. Novel hybrid vectors, yeast hosts transformed with such hybrid vectors and processes for the production thereof are also provided. 77

摘要翻译： 尿素激酶类型的新型人类纤溶酶原激活剂是由用包含编码所述人纤溶酶原激活物的DNA序列的杂交载体转化的酵母细胞产生的。 还提供了新的杂交载体，用这种杂交载体转化的酵母宿主及其制备方法。

公开(公告)号：US5168049A

公开(公告)日：1992-12-01

申请号：US185329

申请日：1988-04-21

申请人： Harry M. Meade ,  Jeffrey L. Garwin

发明人： Harry M. Meade ,  Jeffrey L. Garwin

IPC分类号： C07H21/04 ,  C07K1/16 ,  C07K1/22 ,  C07K14/00 ,  C07K14/36 ,  C07K14/52 ,  C07K14/555 ,  C07K14/575 ,  C07K14/61 ,  C07K14/62 ,  C07K14/76 ,  C07K19/00 ,  C12N1/00 ,  C12N1/21 ,  C12N5/00 ,  C12N9/72 ,  C12N15/00 ,  C12N15/09 ,  C12N15/76 ,  C12P21/02 ,  C12R1/19 ,  C12R1/38 ,  C12R1/465 ,  C12R1/645

CPC分类号： C12N9/6459 ,  C07K14/36 ,  C12N15/76 ,  C12Y304/21069 ,  C07K2319/00 ,  C07K2319/22

摘要： DNA sequences, hybrid DNA sequences, recombinant DNA molecules and processes for producing streptavidin-like polypeptides and for producing fused proteins consisting of a streptavidin-like polypeptide joined end to end with another protein, polypeptide, peptide or amino acid. The DNA sequences, hybrid DNA sequences and recombinant DNA molecules of this invention are characterized in that they include DNA fragments that code for streptavidin-like polypeptides. These DNA sequences, hybrid DNA sequences and recombinant DNA molecules and the hosts transformed with them may be employed in the processes of this invention to produce streptavidin-like polypeptides and fused proteins.

摘要翻译： DNA序列，杂交DNA序列，重组DNA分子和用于产生链霉抗生物素蛋白样多肽和用于产生由链霉抗生物素蛋白样多肽组成的融合蛋白的方法与另一种蛋白质，多肽，肽或氨基酸端到端连接。 本发明的DNA序列，杂交DNA序列和重组DNA分子的特征在于它们包括编码链霉抗生物素蛋白样多肽的DNA片段。 这些DNA序列，杂交DNA序列和重组DNA分子以及用它们转化的宿主可用于本发明的方法中以产生链霉抗生物素蛋白样多肽和融合蛋白。

公开(公告)号：US5122595A

公开(公告)日：1992-06-16

申请号：US354265

申请日：1989-05-19

申请人： Antonio D. Ortega ,  Jose A. Gil ,  Tomas V. Garcia ,  Juan F. Martin

发明人： Antonio D. Ortega ,  Jose A. Gil ,  Tomas V. Garcia ,  Juan F. Martin

IPC分类号： C12N15/03 ,  C07K14/36 ,  C12N15/00 ,  C12N15/31 ,  C12N15/52 ,  C12N15/67 ,  C12N15/76

CPC分类号： C07K14/36

摘要： The cloning and characterization of a newly isolated gene, referred to as saf (secondary metabolism activation factor) is disclosed. This gene encodes a new amino acid polypeptide, referred to as the SAF polypeptide, which directly or indirectly modulates the expression of extracellular enzymes in Steptomyces. DNA units or fragments which encode the SAF polypeptide are also disclosed, as are vectors containing said DNA, host organisms transformed with such vectors, and processes for preparing extracellular enzymes or heterologous polypeptides by culturing such host organisms.

摘要翻译： 公开了新分离的基因的克隆和表征，被称为saf（次级代谢活化因子）。 该基因编码一种称为SAF多肽的新的氨基酸多肽，其直接或间接调节步进样细胞中的细胞外酶的表达。 还公开了编码SAF多肽的DNA单元或片段，以及含有所述DNA的载体，用这些载体转化的宿主生物体，以及通过培养这些宿主生物体制备细胞外酶或异源多肽的方法。

公开(公告)号：US5098837A

公开(公告)日：1992-03-24

申请号：US203387

申请日：1988-06-07

申请人： Robert J. Beckmann ,  Karen L. Cox ,  R. Nagaraja Rao ,  Mark A. Richardson ,  Eugene T. Seno

发明人： Robert J. Beckmann ,  Karen L. Cox ,  R. Nagaraja Rao ,  Mark A. Richardson ,  Eugene T. Seno

IPC分类号： C12N9/00 ,  C12N1/21 ,  C12N15/31 ,  C12N15/52 ,  C12N15/76 ,  C12P19/62 ,  C12R1/465

CPC分类号： C12P19/62 ,  C12N15/52 ,  C12N15/76 ,  Y10S435/886

摘要： Spiramycin antibiotic biosynthetic genes of Streptomyces ambofaciens are provided by the present invention, in addition to a variety of recombinant DNA vectors. The genes also function in other macrolide producing organisms. The genes can be used to increase or otherwise alter the macrolide antibiotic-producing ability of an organism. The present invention also provides host strains comprising mutant spiramycin biosynthetic genes which can be used to generate novel antibiotics. Also provided is a method for preparing the mutant gene comprising mutating cloned spiramycin biosynthetic DNA by transposon mutagenesis with subsequent transformation into a macrolide-antibiotic producing host and homologous recombination into its genome, to generate stable mutant cell lines.

公开(公告)号：US4921801A

公开(公告)日：1990-05-01

申请号：US842102

申请日：1986-03-20

申请人： R. Nagaraja Rao ,  Richard K. Stanzak

发明人： R. Nagaraja Rao ,  Richard K. Stanzak

IPC分类号： C12N1/21 ,  C12N15/76

CPC分类号： C12N15/76 ,  Y10S435/849 ,  Y10S435/889

摘要： Novel recombinant DNA cosmid shuttle vectors and a method of using them in the construction of genomic DNA libraries are described. The vectors demonstrate the incorporation of both the size selection and in vitro packaging mechanisms of lambda into a Streptomyces-E. coli shuttle vector by the incorporation of two or more COS sequences of bacteriophage lambda.

摘要翻译： 描述了新的重组DNA粘粒穿梭载体及其在构建基因组DNA文库中的使用方法。 这些载体证明将λ的大小选择和体外包装机制纳入Streptomyces-E中。 大肠杆菌穿梭载体通过结合两个或多个COS序列的噬菌体λ。