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公开(公告)号：US20100041086A1
公开(公告)日：2010-02-18
申请号：US12497677
申请日：2009-07-05
IPC分类号： C12Q1/34
CPC分类号： C12Q1/34 , B01F13/0071 , B01L3/502784 , B01L7/54 , B01L2300/0654 , B01L2300/0829 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , G01N2021/035 , G01N2333/924 , G01N2800/042
摘要： The invention relates to a microfluidic platform and methods of using the platform for conducting enzyme assays using a droplet actuator. The enzyme assays of the invention are useful for, among other things, identifying and/or characterizing disorders resulting from conditions in which enzymes are defective or are produced in inappropriate amounts. Enzyme assays of the invention may, for example, be used to detect altered activity of a particular enzyme in a sample, which may serve as an indicator of a particular disease. Altered activity may, for example, be caused by conditions which result in the increased or reduced production of a certain enzyme or its substrate and/or conditions which result in defective enzymes and/or substrates exhibiting increased or decreased effectiveness relative to corresponding normal enzymes and/or substrates.
摘要翻译： 本发明涉及一种微流体平台和使用该平台用于使用液滴致动器进行酶测定的方法。 本发明的酶测定法尤其可用于识别和/或表征由酶缺陷或以不适当量产生的病症引起的疾病。 本发明的酶测定可以例如用于检测样品中特定酶的改变的活性，其可以用作特定疾病的指标。 改变的活性可以例如由导致某些酶或其底物的产生增加或减少的条件引起，和/或导致有缺陷的酶和/或底物相对于相应的正常酶显示出增加或降低的有效性的条件，和 /或底物。
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公开(公告)号：US08440392B2
公开(公告)日：2013-05-14
申请号：US12531844
申请日：2008-03-23
IPC分类号： C12Q1/00
CPC分类号： G01N21/6486 , B01F13/0809 , C07H15/04 , C12Q1/34 , G01N2333/928 , G01N2800/042
摘要： A method of conducting a droplet-based enzymatic assay is provided. The method generally makes use of a droplet actuator. A droplet comprising an enzyme of interest is provided on the droplet actuator along with a droplet comprising a substrate which is potentially modified in the presence of the enzyme. The method involves executing droplet operations on the droplet actuator to combine the droplets, thereby yielding an assay droplet, and detecting modification of the substrate by the enzyme in the assay droplet on the droplet actuator. The enzyme of interest may, for example, be a potentially mutated or improperly folded enzyme exhibiting altered enzyme activity as compared to a corresponding normal enzyme.
摘要翻译： 提供了一种进行基于液滴的酶测定的方法。 该方法通常使用液滴致动器。 包含感兴趣的酶的液滴与包含底物的液滴一起提供在液滴致动器上，所述液滴在酶的存在下可能被修饰。 该方法涉及对液滴致动器执行液滴操作以组合液滴，从而产生测定液滴，并且通过液滴致动器上的测定液滴中的酶检测底物的修饰。 感兴趣的酶可以是例如与相应的正常酶相比显示改变的酶活性的潜在突变或不正确折叠的酶。
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公开(公告)号：US08828655B2
公开(公告)日：2014-09-09
申请号：US13862587
申请日：2013-04-15
IPC分类号： C12Q1/00
CPC分类号： G01N21/6486 , B01F13/0809 , C07H15/04 , C12Q1/34 , G01N2333/928 , G01N2800/042
摘要： A method is provided for conducting a droplet-based enzymatic assay, e.g., for diagnostic purposes. On a droplet actuator, a droplet comprising an enzyme of interest is provided along with a droplet comprising a substrate which is potentially modified in the presence of the enzyme. Droplet operations are executed to combine the enzyme and substrate droplets on the droplet actuator, thereby yielding an assay droplet on the droplet actuator. Detecting modification of the substrate by the enzyme in the assay droplet occurs on the droplet actuator. Modified substrate preparations for conducting such enzymatic assays are also provided.
摘要翻译： 提供了用于进行基于液滴的酶测定的方法，例如用于诊断目的。 在液滴致动器上，包含感兴趣的酶的液滴与包含在酶存在下潜在地被修饰的底物的液滴一起提供。 执行液滴操作以将酶和底物液滴组合在液滴致动器上，从而在液滴致动器上产生测定液滴。 在液滴致动器中检测由测定液滴中的酶对底物的修饰。 还提供了用于进行这种酶测定的修饰底物制剂。
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公开(公告)号：US08426213B2
公开(公告)日：2013-04-23
申请号：US12529052
申请日：2008-03-05
IPC分类号： G01N33/50
CPC分类号： C12Q1/28 , C12Q1/005 , C12Q1/006 , G01N2333/904 , Y10T436/118339 , Y10T436/14 , Y10T436/141111 , Y10T436/142222 , Y10T436/143333 , Y10T436/144444 , Y10T436/145555 , Y10T436/146666 , Y10T436/147777 , Y10T436/148888 , Y10T436/206664
摘要： The present invention relates to providing improved hydrogen peroxide assays, as well as droplet actuators for conducting such assays. The droplet actuators of the invention may be used to conduct droplet-based hydrogen peroxide assays. They may also be associated with detectors for analyzing the results of the hydrogen peroxide assays of the invention. They may be provided as components of systems which control droplet operations and/or detection for conducting the hydrogen peroxide assays. Measurement by the detector may be used to quantify the presence of an analyte in a sample.
摘要翻译： 本发明涉及提供改进的过氧化氢测定，以及用于进行这种测定的液滴致动器。 本发明的液滴致动器可用于进行基于液滴的过氧化氢测定。 它们也可以与用于分析本发明的过氧化氢测定结果的检测器相关联。 它们可以作为控制液滴操作和/或检测以进行过氧化氢测定的系统的组件提供。 检测器的测量可用于量化样品中分析物的存在。
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公开(公告)号：US08202686B2
公开(公告)日：2012-06-19
申请号：US12497677
申请日：2009-07-05
IPC分类号： C12Q1/00
CPC分类号： C12Q1/34 , B01F13/0071 , B01L3/502784 , B01L7/54 , B01L2300/0654 , B01L2300/0829 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , G01N2021/035 , G01N2333/924 , G01N2800/042
摘要： The invention relates to a microfluidic platform and methods of using the platform for conducting enzyme assays using a droplet actuator. The enzyme assays of the invention are useful for, among other things, identifying and/or characterizing disorders resulting from conditions in which enzymes are defective or are produced in inappropriate amounts. Enzyme assays of the invention may, for example, be used to detect altered activity of a particular enzyme in a sample, which may serve as an indicator of a particular disease. Altered activity may, for example, be caused by conditions which result in the increased or reduced production of a certain enzyme or its substrate and/or conditions which result in defective enzymes and/or substrates exhibiting increased or decreased effectiveness relative to corresponding normal enzymes and/or substrates.
摘要翻译： 本发明涉及一种微流体平台和使用该平台用于使用液滴致动器进行酶测定的方法。 本发明的酶测定法尤其可用于识别和/或表征由酶缺陷或以不适当量产生的病症引起的疾病。 本发明的酶测定可以例如用于检测样品中特定酶的改变的活性，其可以用作特定疾病的指标。 改变的活性可以例如由导致某些酶或其底物的产生增加或减少的条件引起，和/或导致有缺陷的酶和/或底物相对于相应的正常酶显示出增加或降低的有效性的条件，和 /或底物。
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公开(公告)号：US08927296B2
公开(公告)日：2015-01-06
申请号：US12992939
申请日：2009-05-18
CPC分类号： B01L3/502792 , B01L3/502761 , B01L2200/0673 , B01L2300/0816 , B01L2300/0819 , B01L2300/0829 , B01L2300/089 , B01L2400/0406 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , G01N1/4077 , G01N35/0098 , G01N2035/00564 , Y10T436/25
摘要： The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.
摘要翻译： 本发明提供了液滴致动器和液滴致动器技术。 除其他之外，液滴致动器和方法可用于操纵液滴致动器上的珠，例如使用液滴致动器上的含珠液滴进行液滴操作。 例如，在执行样品制备方案和/或测定方案的上下文中，珠可以在液滴致动器上操作。 本发明方法的输出可以是准备用于执行测定方案的珠粒。 本发明方法的另一个输出可以是使用珠子执行的测定方案的结果。 在本文描述的方法中，将珠粒集中在液滴中的方法，洗涤珠粒的方法，悬浮珠粒的方法，分离珠粒的方法，在液滴内定位珠粒的方法，形成液滴包括珠粒的乳液的方法，加载珠粒的方法 液滴致动器的液滴操作间隙，单层组织珠粒的方法，以及捕获，捕获或抑制珠粒的方法。
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公开(公告)号：US20140057363A9
公开(公告)日：2014-02-27
申请号：US12992939
申请日：2009-05-18
IPC分类号： G01N1/40
CPC分类号： B01L3/502792 , B01L3/502761 , B01L2200/0673 , B01L2300/0816 , B01L2300/0819 , B01L2300/0829 , B01L2300/089 , B01L2400/0406 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , G01N1/4077 , G01N35/0098 , G01N2035/00564 , Y10T436/25
摘要： The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.
摘要翻译： 本发明提供了液滴致动器和液滴致动器技术。 除其他之外，液滴致动器和方法可用于操纵液滴致动器上的珠，例如使用液滴致动器上的含珠液滴进行液滴操作。 例如，在执行样品制备方案和/或测定方案的上下文中，珠可以在液滴致动器上操作。 本发明方法的输出可以是准备用于执行测定方案的珠粒。 本发明方法的另一个输出可以是使用珠子执行的测定方案的结果。 在本文描述的方法中，将珠粒集中在液滴中的方法，洗涤珠粒的方法，悬浮珠粒的方法，分离珠粒的方法，在液滴内定位珠粒的方法，形成液滴包括珠粒的乳液的方法，加载珠粒的方法 液滴致动器的液滴操作间隙，单层组织珠粒的方法，以及捕获，捕获或抑制珠粒的方法。
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公开(公告)号：US20140174926A1
公开(公告)日：2014-06-26
申请号：US14115215
申请日：2012-05-01
申请人： Donovan Bort , Carrie Graham , Vamsee Pamula , Michael Pollack , Ramakrishna Sista , Vijay Srinivasan
发明人： Donovan Bort , Carrie Graham , Vamsee Pamula , Michael Pollack , Ramakrishna Sista , Vijay Srinivasan
IPC分类号： G01N27/447 , B81B1/00
CPC分类号： G01N27/44791 , B01F1/0027 , B01F13/0071 , B01F13/0076 , B01F2001/0055 , B01F2215/0037 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/0668 , B01L2200/10 , B01L2200/16 , B01L2300/0816 , B01L2300/0867 , B01L2300/089 , B01L2400/0406 , B01L2400/0418 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , B81B1/004
摘要： A method of mixing a droplet, the method comprising providing a droplet on a surface, forming the droplet into a first “U” shape having a bottom region and two terminal ends, and simultaneously merging the terminal ends and splitting the droplet at the bottom region to form a second “U” shape which is substantially opposite the first “U” shape.
摘要翻译： 一种混合液滴的方法，所述方法包括在表面上提供液滴，将所述液滴形成为具有底部区域和两个末端的第一“U”形状，并同时合并末端并在底部区域分离液滴 以形成与第一“U”形大体相反的第二“U”形状。
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公开(公告)号：US08658111B2
公开(公告)日：2014-02-25
申请号：US13031760
申请日：2011-02-22
CPC分类号： B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L7/54 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/089 , B01L2300/1827 , B01L2400/0427 , B01L2400/0448 , G01N2035/1046
摘要： The present invention provides droplet actuators, modified fluids and methods relating to droplet operations. An aspect includes a droplet actuator including a droplet operations substrate; an oil based filler fluid on the droplet operations substrate comprising an oil soluble additive in the filler fluid; and a droplet in contact with the oil based filler fluid. Still other aspects are provided.
摘要翻译： 本发明提供了液滴致动器，改进的流体和与液滴操作有关的方法。 一个方面包括具有液滴操作基板的液滴致动器; 在所述液滴操作基底上的油基填充剂流体，其包含在所述填充流体中的油溶性添加剂; 以及与油基填充液接触的液滴。 还提供了其他方面。
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公开(公告)号：US20120261264A1
公开(公告)日：2012-10-18
申请号：US13003765
申请日：2009-07-20
申请人： Vijay Srinivasan , Arjun Sudarsan , Vamsee Pamula , Michael Pollack , Ramakrishna Sista , Raymond Kozikowski , Nicole Bell
发明人： Vijay Srinivasan , Arjun Sudarsan , Vamsee Pamula , Michael Pollack , Ramakrishna Sista , Raymond Kozikowski , Nicole Bell
CPC分类号： G01N27/44756 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L2300/0816 , B01L2300/0819 , B01L2400/0427 , B01L2400/0688 , B01L2400/086 , B29C59/02 , B41J2/1601 , B81B7/0061 , B81B2201/058 , B81B2201/13 , F04B17/00 , F04B19/006
摘要： The invention provides droplet actuators with droplet operations surfaces for manipulating droplets, e.g., by conducting droplet operations. The droplet operations surfaces are typically exposed to a droplet operations gap. One or more regions of a droplet operation surface may include patterned topographic features. The invention also provides a droplet actuator in which one or both gap-facing droplet operations surfaces is formed using a removable film. The removable film may, in various embodiments, also include other components ordinarily associated with the droplet actuator substrate, such as the dielectric layer and the electrodes. Further, the invention provides droplet actuator devices and methods for coupling and/or sealing substrates of a droplet actuator, such as techniques for self-aligning assembly of droplet actuator substrates. The invention provides droplet actuators and methods of disassembling the droplet actuator in order to provide access for cleaning and/or recycling of droplet actuator surfaces.
摘要翻译： 本发明提供了液滴致动器，其具有用于操纵液滴的液滴操作表面，例如通过进行液滴操作。 液滴操作表面通常暴露于液滴操作间隙。 液滴操作表面的一个或多个区域可以包括图案化的地形特征。 本发明还提供一种液滴致动器，其中使用可去除的膜形成一个或两个面向间隙的液滴操作表面。 在各种实施例中，可除去膜可以包括通常与液滴致动器基底（例如电介质层和电极）相关联的其它部件。 此外，本发明提供了液滴致动器装置和用于联接和/或密封液滴致动器的基板的方法，例如用于液滴致动器基板的自对准组装的技术。 本发明提供了液滴致动器和拆卸液滴致动器的方法，以便为液滴致动器表面的清洁和/或回收提供通路。
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