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公开(公告)号:US20140174926A1
公开(公告)日:2014-06-26
申请号:US14115215
申请日:2012-05-01
申请人: Donovan Bort , Carrie Graham , Vamsee Pamula , Michael Pollack , Ramakrishna Sista , Vijay Srinivasan
发明人: Donovan Bort , Carrie Graham , Vamsee Pamula , Michael Pollack , Ramakrishna Sista , Vijay Srinivasan
IPC分类号: G01N27/447 , B81B1/00
CPC分类号: G01N27/44791 , B01F1/0027 , B01F13/0071 , B01F13/0076 , B01F2001/0055 , B01F2215/0037 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/0668 , B01L2200/10 , B01L2200/16 , B01L2300/0816 , B01L2300/0867 , B01L2300/089 , B01L2400/0406 , B01L2400/0418 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , B81B1/004
摘要: A method of mixing a droplet, the method comprising providing a droplet on a surface, forming the droplet into a first “U” shape having a bottom region and two terminal ends, and simultaneously merging the terminal ends and splitting the droplet at the bottom region to form a second “U” shape which is substantially opposite the first “U” shape.
摘要翻译: 一种混合液滴的方法,所述方法包括在表面上提供液滴,将所述液滴形成为具有底部区域和两个末端的第一“U”形状,并同时合并末端并在底部区域分离液滴 以形成与第一“U”形大体相反的第二“U”形状。
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公开(公告)号:US20120261264A1
公开(公告)日:2012-10-18
申请号:US13003765
申请日:2009-07-20
申请人: Vijay Srinivasan , Arjun Sudarsan , Vamsee Pamula , Michael Pollack , Ramakrishna Sista , Raymond Kozikowski , Nicole Bell
发明人: Vijay Srinivasan , Arjun Sudarsan , Vamsee Pamula , Michael Pollack , Ramakrishna Sista , Raymond Kozikowski , Nicole Bell
CPC分类号: G01N27/44756 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L2300/0816 , B01L2300/0819 , B01L2400/0427 , B01L2400/0688 , B01L2400/086 , B29C59/02 , B41J2/1601 , B81B7/0061 , B81B2201/058 , B81B2201/13 , F04B17/00 , F04B19/006
摘要: The invention provides droplet actuators with droplet operations surfaces for manipulating droplets, e.g., by conducting droplet operations. The droplet operations surfaces are typically exposed to a droplet operations gap. One or more regions of a droplet operation surface may include patterned topographic features. The invention also provides a droplet actuator in which one or both gap-facing droplet operations surfaces is formed using a removable film. The removable film may, in various embodiments, also include other components ordinarily associated with the droplet actuator substrate, such as the dielectric layer and the electrodes. Further, the invention provides droplet actuator devices and methods for coupling and/or sealing substrates of a droplet actuator, such as techniques for self-aligning assembly of droplet actuator substrates. The invention provides droplet actuators and methods of disassembling the droplet actuator in order to provide access for cleaning and/or recycling of droplet actuator surfaces.
摘要翻译: 本发明提供了液滴致动器,其具有用于操纵液滴的液滴操作表面,例如通过进行液滴操作。 液滴操作表面通常暴露于液滴操作间隙。 液滴操作表面的一个或多个区域可以包括图案化的地形特征。 本发明还提供一种液滴致动器,其中使用可去除的膜形成一个或两个面向间隙的液滴操作表面。 在各种实施例中,可除去膜可以包括通常与液滴致动器基底(例如电介质层和电极)相关联的其它部件。 此外,本发明提供了液滴致动器装置和用于联接和/或密封液滴致动器的基板的方法,例如用于液滴致动器基板的自对准组装的技术。 本发明提供了液滴致动器和拆卸液滴致动器的方法,以便为液滴致动器表面的清洁和/或回收提供通路。
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公开(公告)号:US08927296B2
公开(公告)日:2015-01-06
申请号:US12992939
申请日:2009-05-18
CPC分类号: B01L3/502792 , B01L3/502761 , B01L2200/0673 , B01L2300/0816 , B01L2300/0819 , B01L2300/0829 , B01L2300/089 , B01L2400/0406 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , G01N1/4077 , G01N35/0098 , G01N2035/00564 , Y10T436/25
摘要: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.
摘要翻译: 本发明提供了液滴致动器和液滴致动器技术。 除其他之外,液滴致动器和方法可用于操纵液滴致动器上的珠,例如使用液滴致动器上的含珠液滴进行液滴操作。 例如,在执行样品制备方案和/或测定方案的上下文中,珠可以在液滴致动器上操作。 本发明方法的输出可以是准备用于执行测定方案的珠粒。 本发明方法的另一个输出可以是使用珠子执行的测定方案的结果。 在本文描述的方法中,将珠粒集中在液滴中的方法,洗涤珠粒的方法,悬浮珠粒的方法,分离珠粒的方法,在液滴内定位珠粒的方法,形成液滴包括珠粒的乳液的方法,加载珠粒的方法 液滴致动器的液滴操作间隙,单层组织珠粒的方法,以及捕获,捕获或抑制珠粒的方法。
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公开(公告)号:US20140057363A9
公开(公告)日:2014-02-27
申请号:US12992939
申请日:2009-05-18
IPC分类号: G01N1/40
CPC分类号: B01L3/502792 , B01L3/502761 , B01L2200/0673 , B01L2300/0816 , B01L2300/0819 , B01L2300/0829 , B01L2300/089 , B01L2400/0406 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , G01N1/4077 , G01N35/0098 , G01N2035/00564 , Y10T436/25
摘要: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.
摘要翻译: 本发明提供了液滴致动器和液滴致动器技术。 除其他之外,液滴致动器和方法可用于操纵液滴致动器上的珠,例如使用液滴致动器上的含珠液滴进行液滴操作。 例如,在执行样品制备方案和/或测定方案的上下文中,珠可以在液滴致动器上操作。 本发明方法的输出可以是准备用于执行测定方案的珠粒。 本发明方法的另一个输出可以是使用珠子执行的测定方案的结果。 在本文描述的方法中,将珠粒集中在液滴中的方法,洗涤珠粒的方法,悬浮珠粒的方法,分离珠粒的方法,在液滴内定位珠粒的方法,形成液滴包括珠粒的乳液的方法,加载珠粒的方法 液滴致动器的液滴操作间隙,单层组织珠粒的方法,以及捕获,捕获或抑制珠粒的方法。
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公开(公告)号:US08658111B2
公开(公告)日:2014-02-25
申请号:US13031760
申请日:2011-02-22
CPC分类号: B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L7/54 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/089 , B01L2300/1827 , B01L2400/0427 , B01L2400/0448 , G01N2035/1046
摘要: The present invention provides droplet actuators, modified fluids and methods relating to droplet operations. An aspect includes a droplet actuator including a droplet operations substrate; an oil based filler fluid on the droplet operations substrate comprising an oil soluble additive in the filler fluid; and a droplet in contact with the oil based filler fluid. Still other aspects are provided.
摘要翻译: 本发明提供了液滴致动器,改进的流体和与液滴操作有关的方法。 一个方面包括具有液滴操作基板的液滴致动器; 在所述液滴操作基底上的油基填充剂流体,其包含在所述填充流体中的油溶性添加剂; 以及与油基填充液接触的液滴。 还提供了其他方面。
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公开(公告)号:US20110091989A1
公开(公告)日:2011-04-21
申请号:US12992939
申请日:2009-05-18
IPC分类号: G01N1/40
CPC分类号: B01L3/502792 , B01L3/502761 , B01L2200/0673 , B01L2300/0816 , B01L2300/0819 , B01L2300/0829 , B01L2300/089 , B01L2400/0406 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , G01N1/4077 , G01N35/0098 , G01N2035/00564 , Y10T436/25
摘要: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.
摘要翻译: 本发明提供了液滴致动器和液滴致动器技术。 除其他之外,液滴致动器和方法可用于操纵液滴致动器上的珠,例如使用液滴致动器上的含珠液滴进行液滴操作。 例如,在执行样品制备方案和/或测定方案的上下文中,珠可以在液滴致动器上操作。 本发明方法的输出可以是准备用于执行测定方案的珠粒。 本发明方法的另一个输出可以是使用珠子执行的测定方案的结果。 在本文描述的方法中,将珠粒集中在液滴中的方法,洗涤珠粒的方法,悬浮珠粒的方法,分离珠粒的方法,在液滴内定位珠粒的方法,形成液滴包括珠粒的乳液的方法,加载珠粒的方法 液滴致动器的液滴操作间隙,单层组织珠粒的方法,以及捕获,捕获或抑制珠粒的方法。
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公开(公告)号:US20110180571A1
公开(公告)日:2011-07-28
申请号:US13031760
申请日:2011-02-22
IPC分类号: B67D7/06
CPC分类号: B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L7/54 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/089 , B01L2300/1827 , B01L2400/0427 , B01L2400/0448 , G01N2035/1046
摘要: The present invention provides droplet actuators, modified fluids and methods relating to droplet operations. An aspect includes a droplet actuator including a droplet operations substrate; an oil based filler fluid on the droplet operations substrate comprising an oil soluble additive in the filler fluid; and a droplet in contact with the oil based filler fluid. Still other aspects are provided.
摘要翻译: 本发明提供了液滴致动器,改进的流体和与液滴操作有关的方法。 一个方面包括具有液滴操作基板的液滴致动器; 在所述液滴操作基底上的油基填充剂流体,其包含在所述填充流体中的油溶性添加剂; 以及与油基填充液接触的液滴。 还提供了其他方面。
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公开(公告)号:US20080044893A1
公开(公告)日:2008-02-21
申请号:US11839774
申请日:2007-08-16
申请人: Michael Pollack , Vamsee Pamula , Vijay Srinivasan
发明人: Michael Pollack , Vamsee Pamula , Vijay Srinivasan
CPC分类号: B01L3/502784 , B01J19/0046 , B01J2219/00466 , B01J2219/00495 , B01J2219/00587 , B01J2219/00725 , B01L3/502761 , B01L2200/0642 , B01L2200/0647 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/0887 , B01L2300/0893 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07K1/1136 , C30B7/00 , C30B29/58 , Y10T436/2575
摘要: Multiwell droplet actuators, systems and methods are provided. According to one embodiment, a substrate is provided and comprises: (a) one or more input ports for introduction of one or more reagents and/or samples; (b) a regular array of processing wells; and (c) a network of droplet transport pathways comprising pathways that provide direct or indirect droplet transport from each of the input ports to each of the one or more processing wells. Varying droplet actuators and systems related thereto are also provided.
摘要翻译: 提供多孔液滴致动器,系统和方法。 根据一个实施例,提供了一种衬底,其包括:(a)用于引入一种或多种试剂和/或样品的一个或多个输入端口; (b)一系列加工井; 和(c)液滴传输路径的网络,其包括提供从每个输入端口到所述一个或多个处理井中的每一个的直接或间接液滴传输的路径。 还提供了与其相关的不同液滴致动器和系统。
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公开(公告)号:US20080053205A1
公开(公告)日:2008-03-06
申请号:US11639566
申请日:2006-12-15
申请人: Michael Pollack , Vamsee Pamula , Vijay Srinivasan
发明人: Michael Pollack , Vamsee Pamula , Vijay Srinivasan
CPC分类号: G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
摘要: The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
摘要翻译: 本发明涉及基于液滴的颗粒分选。 根据一个实施例,提供了液滴微致动器,其包括:(a)颗粒悬浮液; 和(b)布置成使用包含颗粒的液滴进行液滴操作的电极。 还提供了输送颗粒的方法,其中所述方法包括提供包含颗粒的液滴并将液滴输送到液滴微致动器上。
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公开(公告)号:US20080050834A1
公开(公告)日:2008-02-28
申请号:US11839762
申请日:2007-08-16
申请人: Vamsee Pamula , Vijay Srinivasan , Michael Pollack
发明人: Vamsee Pamula , Vijay Srinivasan , Michael Pollack
CPC分类号: G01N23/20 , B01L3/06 , B01L3/502792 , B01L2200/16 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0427 , Y10T436/25
摘要: Protein crystallization droplet actuators, systems and methods are provided. According to one embodiment, a droplet actuator for providing an array of crystallization conditions is provided and includes: (a) two or more processing reservoirs; (b) two or more dispensing reservoirs collectively comprising two or more crystallization reagents; and (c) a port for introducing a sample for crystallization analysis. Systems including the droplet actuator, methods of providing an array of crystallization conditions, and methods of identifying crystallization conditions are also provided.
摘要翻译: 提供蛋白质结晶液滴致动器,系统和方法。 根据一个实施例,提供了一种用于提供结晶条件阵列的液滴致动器,其包括:(a)两个或更多个处理容器; (b)两个或更多个分配容器,共同包含两种或多种结晶试剂; 和(c)用于引入用于结晶分析的样品的端口。 还提供了包括液滴致动器,提供结晶条件阵列的方法以及鉴定结晶条件的方法的系统。
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