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863 条记录 (耗时 0.061 秒)

    Method for Late Introduction of the (8R)-Hydroxyl Group Carbapenem Beta-Lactam Antibiotic Synthesis
    78.
    发明申请
    Method for Late Introduction of the (8R)-Hydroxyl Group Carbapenem Beta-Lactam Antibiotic Synthesis 有权
    （8R） - 羟基卡巴因内β-内酰胺抗生素合成方法

    公开(公告)号：US20130066066A1

    公开(公告)日：2013-03-14

    申请号：US13515114

    申请日：2010-12-13

    申请人： Craig Arthur Townsend ,  Micah Jeffrey Bodner ,  Ryan Martin Phelan ,  Michael Francis Freeman

    发明人： Craig Arthur Townsend ,  Micah Jeffrey Bodner ,  Ryan Martin Phelan ,  Michael Francis Freeman

    IPC分类号： C12P17/10 ,  C07D477/00

    CPC分类号： C12P17/10 ,  C07D477/20 ,  C12P17/184

    摘要： The presently disclosed subject matter demonstrates that ThnG and ThnQ enzymes encoded by the thienamycin gene cluster in Streptomyces cattleya oxidize the C-2 and C-6 moieties of carbapenems, respectively. ThnQ stereospecifically hydroxylates PS-5 giving N-acetyl thienamycin. ThnG catalyzes sequential desaturation and sulfoxidation of PS-5, giving PS-7 and its sulfoxide. The ThnG and ThnQ enzymes are relatively substrate selective, but give rise to the oxidative diversity of carbapenems produced by S. cattleya, and orthologues likely function similarly in allied streptomyces.

    摘要翻译： 目前公开的主题表明，由链霉菌链霉菌中的噻吩霉素基因簇编码的ThnG和ThnQ酶分别氧化碳青霉烯的C-2和C-6部分。 ThnQ立体选择性羟基化PS-5给予N-乙酰基噻吩霉素。 ThnG催化PS-5的顺序去饱和和亚磺氧化，得到PS-7及其亚砜。 ThnG和ThnQ酶是相对底物选择性的，但导致由S. cattleya产生的碳青霉烯类的氧化多样性，直系同源物在相关的链霉菌中可能具有相似的功能。

    Microorganisms and methods for the biosynthesis of adipate, hexamethylenediamine and 6-aminocaproic acid
    79.
    发明授权
    Microorganisms and methods for the biosynthesis of adipate, hexamethylenediamine and 6-aminocaproic acid 有权
    用于生物合成己二酸，六亚甲基二胺和6-氨基己酸的微生物和方法

    公开(公告)号：US08377680B2

    公开(公告)日：2013-02-19

    申请号：US12776365

    申请日：2010-05-07

    申请人： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

    发明人： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

    IPC分类号： C12N1/20 ,  C12N1/00 ,  C12N15/70 ,  C12P7/42 ,  C12P13/00 ,  C12P17/10 ,  C07H21/04

    CPC分类号： C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/12

    摘要： The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

    摘要翻译： 本发明提供了具有6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径的非天然存在的微生物生物。 微生物生物体含有至少一种编码各自的6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径中的酶的外源核酸。 本发明另外提供了制备6-氨基己酸，己内酰胺，己二胺或乙酰丙酸的方法。 该方法可以包括培养6-氨基己酸，己内酰胺或六亚甲基二胺生产微生物，其中微生物生物体表达至少一种编码6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径酶的外源核酸，其量足以产生 各自的产物在条件下和足够的时间内产生6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸。