公开(公告)号：US20160139015A1

公开(公告)日：2016-05-19

申请号：US14948119

申请日：2015-11-20

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Soojung C. Hur ,  Albert J. Mach

IPC: G01N1/40 ,  G01N33/50

CPC classification number: C12Q1/24 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/082 ,  B01L2400/086 ,  C12M23/16 ,  C12M29/00 ,  C12M47/04 ,  C12Q1/68 ,  G01N1/40 ,  G01N1/4077 ,  G01N33/5091 ,  G01N33/582

Abstract: A method of isolating cells includes providing a microfluidic device having at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel comprises at least one expansion region disposed along the length thereof. The at least one expansion region is an abrupt increase in a cross-sectional dimension of the at least one microfluidic channel configured to generate a vortex within the at least one expansion region in response to fluid flow. A solution containing a population of cells at least some of which have diameters ≧10 μm flows into the inlet. A portion of cells is trapped within vortex created within the at least one expansion region. The trapped cells may then released from the expansion region.

公开(公告)号：US20250161933A1

公开(公告)日：2025-05-22

申请号：US19030825

申请日：2025-01-17

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Chueh-Yu Wu

IPC: B01L3/02 ,  B01L3/00 ,  C12N1/04 ,  C12N5/071 ,  C12N5/0783 ,  C12Q1/6806 ,  G01N33/543

Abstract: Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and/or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and/or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.

公开(公告)号：US20250161932A1

公开(公告)日：2025-05-22

申请号：US19030466

申请日：2025-01-17

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Chueh-Yu Wu

IPC: B01L3/02 ,  B01L3/00 ,  C12N1/04 ,  C12N5/071 ,  C12N5/0783 ,  C12Q1/6806 ,  G01N33/543

Abstract: Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and/or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and/or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.

公开(公告)号：US20250155355A1

公开(公告)日：2025-05-15

申请号：US19025994

申请日：2025-01-16

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Daniel R. Gossett ,  Henry T.K. Tse

IPC: G01N15/1433 ,  B01L3/00 ,  C12Q1/02 ,  G01N15/01 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/149 ,  G01N33/487 ,  G01N33/574 ,  G16B99/00

Abstract: A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.

公开(公告)号：US20230293438A1

公开(公告)日：2023-09-21

申请号：US18015863

申请日：2021-07-15

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Jun Fang ,  Song Li ,  Dino Di Carlo ,  Jaekyung Koh

IPC: A61K9/16 ,  A61K9/06 ,  A61K31/352 ,  A61K31/444 ,  A61P9/10 ,  A61K47/10

CPC classification number: A61K9/167 ,  A61K9/06 ,  A61K9/1647 ,  A61K31/352 ,  A61K31/444 ,  A61K47/10 ,  A61P9/10

Abstract: A therapeutic microporous hydrogel scaffold for use in an animal is disclosed that releases one or more therapeutic agents or drugs. The scaffold uses a drug-releasing microporous annealed particle system that encapsulates drug-loaded nanoparticles into particle building blocks. By modulating nanoparticle hydrophilicity and pre-gel solution viscosity, the particle building blocks were generated with consistent and homogeneous encapsulation of nanoparticles. The scaffold may be used to treat myocardial infarction (MI) using, for example, the drugs forskolin (F) and Repsox (R). The intramyocardial injection of the pre-annealed hydrogel slurry of particles that formed the resultant scaffold improved left ventricular functions, which were further enhanced with increased angiogenesis and reduced fibrosis and inflammatory response. This therapeutic microporous hydrogel scaffold platform represents a new generation of microgel particles for MI therapy and will have broad applications in regenerative medicine and disease therapy.

公开(公告)号：US11697833B2

公开(公告)日：2023-07-11

申请号：US16464681

申请日：2017-11-29

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Omai Garner ,  Dino Di Carlo ,  Steve Wei Feng

IPC: G01N21/25 ,  C12M1/32 ,  C12M1/34 ,  C12Q1/18 ,  G06V10/12 ,  G06V20/69 ,  G01N21/64

CPC classification number: C12Q1/18 ,  G01N21/253 ,  G06V10/12 ,  G06V20/69 ,  G01N2201/0221 ,  G01N2201/0833

Abstract: A method of performing antimicrobial susceptibility testing (AST) on a sample uses a reader device that mounts on a mobile phone having a camera. A microtiter plate containing wells preloaded with the bacteria-containing sample, growth medium, and drugs of differing concentrations is loaded into the reader device. The wells are illuminated using an array of illumination sources contained in the reader device. Images of the wells are acquired with the camera of the mobile phone. In one embodiment, the images are transmitted to a separate computing device for processing to classify each well as turbid or not turbid and generating MIC values and a susceptibility characterization for each drug in the panel based on the turbidity classification of the array of wells. The MIC values and the susceptibility characterizations for each drug are transmitted or returned to the mobile phone for display thereon.

公开(公告)号：US11590489B2

公开(公告)日：2023-02-28

申请号：US16550105

申请日：2019-08-23

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Chueh-Yu Wu

IPC: B01L3/02 ,  B01L3/00 ,  G01N33/543 ,  C12N1/04 ,  C12N5/071 ,  C12N5/0783 ,  C12Q1/6806

Abstract: Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and/or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and/or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.

公开(公告)号：US20220379309A1

公开(公告)日：2022-12-01

申请号：US17770959

申请日：2020-10-21

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Sam Emaminejad ,  Dino Di Carlo ,  Wenzhuo Yu ,  Haisong Lin ,  Yilian Wang

IPC: B01L3/00 ,  G01N15/14 ,  G01N15/10

Abstract: An electronically-controlled digital ferrofluidic device is disclosed which employs a network of individually addressable coils in conjunction with one or more movable permanent magnets, where each moveable permanent magnet delivers the designated fluid manipulation-based tasks. The underlying mechanism facilitating fluidic operations is realized by addressable electromagnetic actuation of miniaturized mobile magnets that exert localized magnetic body forces on droplets filled with magnetic nanoparticles. The reconfigurable, contactless, and non-interfering magnetic-field operation properties of the underlying actuation mechanism allow for the integration of passive and active components to implement advanced and diverse operations with high efficiency (e.g., droplet sorting, dispensing, generation, merging, mixing, filtering, and analysis).

公开(公告)号：US11464886B2

公开(公告)日：2022-10-11

申请号：US17144158

申请日：2021-01-08

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Donald R. Griffin ,  Westbrook Weaver ,  Tatiana Segura ,  Dino Di Carlo ,  Philip Scumpia

IPC: A61L26/00 ,  A61L27/18 ,  A61L27/58 ,  A61K47/62 ,  A61K9/06 ,  A61K31/795 ,  A61L27/22 ,  A61L27/52 ,  A61L27/54 ,  A61L27/56

Abstract: A microporous gel system for certain applications, including biomedical applications, includes an aqueous solution containing plurality of microgel particles including a biodegradable crosslinker. In some aspects, the microgel particles act as gel building blocks that anneal to one another to form a covalently-stabilized scaffold of microgel particles having interstitial spaces therein. In certain aspects, annealing of the microgel particles occurs after exposure to an annealing agent that is endogenously present or exogenously added. In some embodiments, annealing of the microgel particles requires the presence of an initiator such as exposure to light. In particular embodiments, the chemical and physical properties of the gel building blocks can be controlled to allow downstream control of the resulting assembled scaffold. In one or more embodiments, cells are able to quickly infiltrate the interstitial spaces of the assembled scaffold.

公开(公告)号：US20220299525A1

公开(公告)日：2022-09-22

申请号：US17612575

申请日：2020-05-22

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Hyou-Arm Joung ,  Zachary S. Ballard ,  Omai Garner ,  Dino Di Carlo ,  Artem Goncharov

IPC: G01N33/68 ,  G01N33/543 ,  G06T7/00

Abstract: A system for detecting the presence of and/or quantifying the amount or concentration of one or more analytes in a sample includes a flow assay cartridge having a multiplexed sensing membrane that has immunoreaction or biological reaction spots of varying conditions spatially arranged across the surface of the membrane defining an optimized spot map. A reader device is provided that uses a camera to image the multiplexed sensing membrane. Image processing software obtains normalized pixel intensity values of the plurality of immunoreaction or biological reaction spots and which are used as inputs to one or more trained neural networks configured to generate one or more outputs that: (i) quantify the amount or concentration of the one or more analytes in the sample; and/or (ii) indicate the presence of the one or more analytes in the sample; and/or (ii) determines a diagnostic decision or classification of the sample.