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    SURFACE LINKERS FOR ARRAY SYNTHESIS
    1.
    发明申请
    SURFACE LINKERS FOR ARRAY SYNTHESIS 审中-公开
    表面连接器用于阵列合成

    公开(公告)号：US20130165350A1

    公开(公告)日：2013-06-27

    申请号：US13335677

    申请日：2011-12-22

    申请人： Robert Kuimelis ,  Glenn McGall ,  Dexter Pao ,  Zihui Chen ,  Trevor Axelrod

    发明人： Robert Kuimelis ,  Glenn McGall ,  Dexter Pao ,  Zihui Chen ,  Trevor Axelrod

    IPC分类号： C40B50/18

    CPC分类号： C40B50/18 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725

    摘要： The present invention provide several methods of derivatizing a surface of a support with one or more linkers thus providing a suitable platform for synthesis of a polymer array, particular a nucleic acid array. Some methods derivatize a surface with a self-assembled monolayer (SAM) of a linker. The SAM confers advantages of hydrolytic stability, broad compatibility with synthesis and detection chemistries, and reduced emergence of latent functional groups during polymer array synthesis. Substrates can also be derivatized with multi-layers of SAMs providing greater hydrolytic stability. Substrates can also be derivatized by synthesizing a linker in situ on the substrate by atom transfer radical polymerization of functional and functional monomers. Appropriate selection of monomers reduces emergence of latent functional groups in subsequent array synthesis.

    摘要翻译： 本发明提供了用一种或多种接头衍生载体表面的几种方法，从而提供合成聚合物阵列，特别是核酸阵列的合适平台。 一些方法用接头的自组装单层（SAM）衍生表面。 SAM赋予水解稳定性的优点，与合成和检测化学物质的广泛相容性，以及降低聚合物阵列合成期间潜在官能团的出现。 底物也可以通过提供更好的水解稳定性的多层SAM衍生化。 也可以通过功能单体和官能单体的原子转移自由基聚合在底物上原位合成接头来衍生底物。 合适的单体选择减少后续阵列合成中潜在官能团的出现。

    Nucleic Acid Labeling Compounds
    2.
    发明申请
    Nucleic Acid Labeling Compounds 有权
    核酸标记化合物

    公开(公告)号：US20110082289A1

    公开(公告)日：2011-04-07

    申请号：US12892531

    申请日：2010-09-28

    申请人： Glenn McGall ,  Anthony D. Barone

    发明人： Glenn McGall ,  Anthony D. Barone

    IPC分类号： C07H19/06 ,  C07D413/04

    CPC分类号： C12Q1/6816 ,  C07D239/47 ,  C07H7/00 ,  C07H7/06 ,  C07H19/06

    摘要： Nucleic acid labeling compounds are disclosed. The compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.

    摘要翻译： 公开了核酸标记化合物。 通过将杂环衍生物与环状基团（例如呋喃核糖衍生物）缩合来合成化合物。 标记化合物适合于在末端或内部酶促附着于核酸，以提供核酸检测机制。

    Functionalized silicon compounds and methods for their synthesis and use
    5.
    发明授权
    Functionalized silicon compounds and methods for their synthesis and use 有权

    公开(公告)号：US07129308B2

    公开(公告)日：2006-10-31

    申请号：US10857247

    申请日：2004-05-28

    申请人： Glenn McGall ,  Jonathan Eric Forman

    发明人： Glenn McGall ,  Jonathan Eric Forman

    IPC分类号： C08G77/00 ,  C08G77/04

    CPC分类号： C08G73/0206 ,  C03C17/30 ,  C03C2218/113 ,  C07F7/1804 ,  C09D179/02 ,  C09D183/08 ,  C09D183/14 ,  Y10T428/31663

    摘要： Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.

    Functionalized silicon compounds and methods for their synthesis and use
    6.
    发明授权
    Functionalized silicon compounds and methods for their synthesis and use 有权

    公开(公告)号：US07125947B2

    公开(公告)日：2006-10-24

    申请号：US10857329

    申请日：2004-05-28

    申请人： Glenn McGall ,  Jonathan Eric Forman

    发明人： Glenn McGall ,  Jonathan Eric Forman

    IPC分类号： C08G77/00 ,  C08G77/04

    CPC分类号： C08G73/0206 ,  C03C17/30 ,  C03C2218/113 ,  C07F7/1804 ,  C09D179/02 ,  C09D183/08 ,  C09D183/14 ,  Y10T428/31663

    摘要： Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.

    Nucleic acid labeling methods
    7.
    发明申请
    Nucleic acid labeling methods 有权

    公开(公告)号：US20060160096A1

    公开(公告)日：2006-07-20

    申请号：US10983046

    申请日：2004-11-04

    申请人： Kyle Cole ,  Vivi Truong ,  Glenn McGall ,  Anthony Barone

    发明人： Kyle Cole ,  Vivi Truong ,  Glenn McGall ,  Anthony Barone

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C07H19/20 ,  C07H19/10 ,  C07H21/00

    摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3′-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3′-AppN-3′-linker-detectable moiety is used as donor molecule. In another aspect of the present invention, a method of detecting the presence of an RNA of interest in a sample is provided, the method having the following steps: providing the sample comprising RNA which may or may not have said RNA of interest; treating the sample with a fragmenting reagent to provide RNA fragments; removing phosphate groups from said fragments to provide fragments with free 3′ OH groups; ligating said fragment with a labeling reagent according to the instant invention; providing a nucleic acid array having probes directed to said RNA of interest; hybridizing the labeled nucleic acid fragments to said nucleic acid array; and determining the extent of hybridization to said probes to determine the presence of said RNA of interest.