公开(公告)号：US20110086494A1

公开(公告)日：2011-04-14

申请号：US12899338

申请日：2010-10-06

申请人： Noritomo Mitsugi ,  Masataka Hourai ,  Shuichi Samata ,  Kiyoshi Nagai ,  Kei Matsumoto

发明人： Noritomo Mitsugi ,  Masataka Hourai ,  Shuichi Samata ,  Kiyoshi Nagai ,  Kei Matsumoto

IPC分类号： H01L21/322

CPC分类号： H01L21/3221

摘要： To provide a method of removing a heavy metal contained in a thinned semiconductor substrate.A method of removing a heavy metal in a semiconductor substrate of the present invention comprises: attaching, to a rear surface of the semiconductor substrate, a material that lowers a potential barrier of the rear surface of the semiconductor substrate, on a front surface of which a circuit is to be formed or is formed; applying a thermal treatment to the semiconductor substrate under a condition based on a thickness and a resistivity of the semiconductor substrate; and, depositing the heavy metal in the semiconductor substrate on the rear surface.

摘要翻译： 提供一种除去减薄的半导体衬底中所含的重金属的方法。 本发明的半导体衬底中的去除重金属的方法包括：将半导体衬底的后表面的势垒降低到半导体衬底的背面的材料的前表面 要形成或形成电路; 在基于半导体衬底的厚度和电阻率的条件下对半导体衬底进行热处理; 并且将重金属沉积在半导体衬底的后表面上。

公开(公告)号：US07767969B2

公开(公告)日：2010-08-03

申请号：US12319332

申请日：2009-01-06

申请人： Kiyoshi Nagai ,  Harumi Shibata ,  Sayaka Hamaguchi

发明人： Kiyoshi Nagai ,  Harumi Shibata ,  Sayaka Hamaguchi

IPC分类号： G01J5/06

CPC分类号： G01J3/45 ,  G01N21/3563 ,  G01N2021/3568 ,  G01N2021/3595

摘要： An object of the present invention is to provide a spectroscopic method and an apparatus which can measure a trace element accurately with high sensitivity. In order to achieve this object, for example, in Fourier transformation infrared spectroscopy (FT-IR), a reference spectrum and a measurement spectrum including an impurity spectrum are measured in order to obtain a differential spectrum comprising the impurity spectrum and a flat baseline, correction including a frequency shift of the reference spectrum before calculating a differential spectrum, is performed on the reference spectrum. This makes it possible to remove baseline deformation due to phonon absorbance of silicon included in the conventional differential spectrum, and to obtain an infrared absorption spectrum of the substitutional carbon with high accuracy and high sensitivity.

摘要翻译： 本发明的目的是提供一种能够以高灵敏度精确地测量微量元素的分光方法和装置。 为了实现该目的，例如在傅里叶变换红外光谱（FT-IR）中，测量参考光谱和包含杂质光谱的测量光谱，以获得包含杂质光谱和平坦基线的差分光谱， 在参考光谱上执行包括计算差分光谱之前的参考光谱的频移的校正。 这使得可以消除由常规差分光谱中包含的硅的声子吸收引起的基线变形，并以高精度和高灵敏度获得取代碳的红外吸收光谱。

公开(公告)号：US20090173884A1

公开(公告)日：2009-07-09

申请号：US12319332

申请日：2009-01-06

申请人： Kiyoshi Nagai ,  Harumi Shibata ,  Sayaka Hamaguchi

发明人： Kiyoshi Nagai ,  Harumi Shibata ,  Sayaka Hamaguchi

IPC分类号： G01J5/02 ,  G01J3/45

CPC分类号： G01J3/45 ,  G01N21/3563 ,  G01N2021/3568 ,  G01N2021/3595

摘要： An object of the present invention is to provide a spectroscopic method and an apparatus which can measure a trace element accurately with high sensitivity. In order to achieve this object, for example, in Fourier transformation infrared spectroscopy (FT-IR), a reference spectrum and a measurement spectrum including an impurity spectrum are measured in order to obtain a differential spectrum comprising the impurity spectrum and a flat baseline, correction including a frequency shift of the reference spectrum before calculating a differential spectrum, is performed on the reference spectrum. This makes it possible to remove baseline deformation due to phonon absorbance of silicon included in the conventional differential spectrum, and to obtain an infrared absorption spectrum of the substitutional carbon with high accuracy and high sensitivity.

摘要翻译： 本发明的目的是提供一种能够以高灵敏度精确地测量微量元素的分光方法和装置。 为了实现该目的，例如在傅里叶变换红外光谱（FT-IR）中，测量参考光谱和包含杂质光谱的测量光谱，以获得包含杂质光谱和平坦基线的差分光谱， 在参考光谱上执行包括计算差分光谱之前的参考光谱的频移的校正。 这使得可以消除由常规差分光谱中包含的硅的声子吸收引起的基线变形，并以高精度和高灵敏度获得取代碳的红外吸收光谱。

公开(公告)号：US06828125B1

公开(公告)日：2004-12-07

申请号：US08453485

申请日：1995-05-30

申请人： Stephen J. Hoffman ,  Douglas L. Looker ,  Kiyoshi Nagai

发明人： Stephen J. Hoffman ,  Douglas L. Looker ,  Kiyoshi Nagai

IPC分类号： C12P2106

CPC分类号： C07K14/805 ,  A61K38/00 ,  A61K47/6445 ,  C07K2319/00 ,  C12N15/81

摘要： The alpha subunits of hemoglobin, which in nature are formed as separate polypeptide chains which bind noncovalently to the beta subunits, are here provided in the form of the novel molecule di-alpha globin, a single polypeptide chain defined by connecting the two alpha subunits either directly via peptide bond or indirectly by a flexible amino acid or peptide linker. Di-alpha globin may be combined in vivo or in vitro with beta globin and heme to form hemoglobin. Di-alpha globin is expressed by recombinant DNA techniques. Di-beta globin may be similarly obtained.

摘要翻译： 在本文中，血红蛋白的α亚基本质上被形成为与β-亚基非共价结合的分离的多肽链，这里以新分子二-α球蛋白的形式提供，单链多肽链通过将两个α亚基连接而定义 直接通过肽键或通过柔性氨基酸或肽接头间接。 Di-α珠蛋白可以在体内或体外与β球蛋白和血红素组合以形成血红蛋白。 Di-α球蛋白通过重组DNA技术表达。 可以类似地获得二β-珠蛋白。

公开(公告)号：US6010883A

公开(公告)日：2000-01-04

申请号：US854749

申请日：1992-03-19

申请人： Kiyoshi Nagai ,  Hans Christian Th.o slashed.gersen

发明人： Kiyoshi Nagai ,  Hans Christian Th.o slashed.gersen

IPC分类号： C12N15/09 ,  A61K35/74 ,  A61K38/00 ,  C07H21/04 ,  C07K1/22 ,  C07K14/00 ,  C07K14/195 ,  C07K14/41 ,  C07K14/47 ,  C07K14/575 ,  C07K14/585 ,  C07K14/805 ,  C07K19/00 ,  C12N1/20 ,  C12N1/21 ,  C12N9/00 ,  C12N9/10 ,  C12N15/00 ,  C12N15/62 ,  C12P21/00 ,  C12P21/02 ,  C12R1/125 ,  C12R1/19 ,  C12N15/12 ,  C12N15/13 ,  C12N15/14 ,  C12N15/74

CPC分类号： C12N9/1033 ,  C07K14/4716 ,  C07K14/585 ,  C07K14/805 ,  C12N15/62 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/61 ,  C07K2319/75

摘要： This invention provides a DNA sequence coding for a cleavage site which is specifically cleaved by blood coagulation Factor Xa, a vector containing such a sequence, and a host organism transformed with such a vector. Preferably, in the vector, the Factor Xa cleavage site coding sequence is fused at one end to a product and at its other end to an ATG codon or a sequence coding for at least part of a host protein.This invention also provides a process, for the production of a desired protein or peptide product in native form, comprising:transforming a host organism with a vector as described above;expressing the desired protein or peptide product as a fusion protein comprising the desired protein or peptide product fused to a Factor Xa cleavage site; anda cleaving the fusion protein with Factor Xa to yield the foreign gene product in native form.

摘要翻译： 本发明提供编码由凝血因子Xa特异性切割的切割位点的DNA序列，含有该序列的载体和用该载体转化的宿主生物体。 优选地，在载体中，因子Xa切割位点编码序列在一端与产物融合，而在另一端与ATG密码子或编码至少部分宿主蛋白的序列融合。 本发明还提供了一种用于生产天然形式的所需蛋白质或肽产物的方法，其包括：用如上所述的载体转化宿主生物; 表达所需的蛋白质或肽产物作为融合蛋白，其包含与因子Xa切割位点融合的所需蛋白质或肽产物; 并用因子Xa切割融合蛋白以产生天然形式的外源基因产物。

公开(公告)号：US5844088A

公开(公告)日：1998-12-01

申请号：US444991

申请日：1995-05-19

申请人： Stephen J. Hoffman ,  Douglas L. Looker ,  Mary S. Rosendahl ,  Gary L. Stetler ,  Michael Wagenbach ,  David C. Anderson ,  Antony James Mathews ,  Kiyoshi Nagai

发明人： Stephen J. Hoffman ,  Douglas L. Looker ,  Mary S. Rosendahl ,  Gary L. Stetler ,  Michael Wagenbach ,  David C. Anderson ,  Antony James Mathews ,  Kiyoshi Nagai

IPC分类号： A61K38/16 ,  A61K38/00 ,  A61K47/48 ,  A61P7/08 ,  C07K20060101 ,  C07K1/113 ,  C07K14/805 ,  C12N15/00 ,  C12N15/09 ,  C12N15/12 ,  C12N15/81 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91

CPC分类号： C07K14/805 ,  A61K47/48307 ,  C12N15/81 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/92

摘要： The alpha subunits of hemoglobin, which in nature are formed as separate polypeptide chains which bind noncovalently to the beta subunits, are here provided in the form of the novel molecule di-alpha globin, a single polypeptide chain defined by connecting the two alpha subunits either directly via peptide bond or indirectly by a flexible amino acid or peptide linker. Di-alpha globin may be combined in vivo or in vitro with beta globin and heme to form hemoglobin. Di-alpha globin is expressed by recombinant DNA techniques. Di-beta globin may be similarly obtained. We further describe the production of tetrameric human hemoglobin and di-alpha/beta.sub.2 hemoglobin in the yeast Saccharomyces cerevisiae. The synthesis of the protein is directed by a synthetic promotor consisting of two functional parts, an upstream activator sequence (UAS) that confers inducible transcription by galactose from a consensus yeast transcriptional initiation site. The expression construct is designed such that translation is expected to initiate at the same position as the human wild-type genes for .alpha.- and .beta.-globin. Three different types of expression vectors have been used: (1) .alpha.-globin and .beta.-globin contained on two separate plasmids (pGS4688 and pGS4988) in a diploid yeast strain; (2) .alpha.-globin and .beta.-globin each contained on a single plasmid (pGS289 and pGS389) and expressed in either haploid or diploid strains; and (3) di-alpha-globin and beta globin contained on a single plasmid (pGS 3089)and expressed in haploid strains. Finally, we describe the co-expression of alpha and beta globin chains. The chains are folded together and combined intracellularly with heme to form active tetrameric hemoglobin. The hemoglobin may be recovered from the cells' soluble fraction. The invention thus obviates the need to express alpha and beta globin separately, solubilize, renature and purify them, and combine them in vitro with heme to obtain an artificial hemoglobin. By way of comparison, the separately expressed beta globin known in the art is deposited in inclusion bodies. Polycistronic co-expression of alpha and beta globins is particularly preferred.

公开(公告)号：US5716969A

公开(公告)日：1998-02-10

申请号：US781204

申请日：1997-01-09

申请人： Takanobu Naito ,  Haruhito Kobayashi ,  Hironobu Ogura ,  Kiyoshi Nagai ,  Tokiko Nishida ,  Tadashi Arika ,  Mamoru Yokoo ,  Satoko Shusse

发明人： Takanobu Naito ,  Haruhito Kobayashi ,  Hironobu Ogura ,  Kiyoshi Nagai ,  Tokiko Nishida ,  Tadashi Arika ,  Mamoru Yokoo ,  Satoko Shusse

IPC分类号： C07D521/00 ,  A61K31/445 ,  C07D401/06 ,  C07D403/06

CPC分类号： C07D231/12 ,  C07D233/56 ,  C07D249/08

摘要： There is disclosed a fungicide containing, as an effective ingredient, a compound having the general formula (I): ##STR1## or an acid addition salt thereof, particularly the compound wherein an absolute configuration of the asymmetric carbon atoms is R,R-configuration or an acid addition salt thereof.

摘要翻译： 公开了含有具有通式（I）的化合物或其酸加成盐作为有效成分的杀真菌剂，特别是其中不对称碳原子的绝对构型为R的化合物， R构型或其酸加成盐。

公开(公告)号：US5620994A

公开(公告)日：1997-04-15

申请号：US532800

申请日：1995-11-07

申请人： Takanobu Naito ,  Haruhito Kobayashi ,  Hironobu Ogura ,  Kiyoshi Nagai ,  Tokiko Nishida ,  Tadashi Arika ,  Mamoru Yokoo ,  Satoko Shusse

发明人： Takanobu Naito ,  Haruhito Kobayashi ,  Hironobu Ogura ,  Kiyoshi Nagai ,  Tokiko Nishida ,  Tadashi Arika ,  Mamoru Yokoo ,  Satoko Shusse

IPC分类号： C07D521/00 ,  A61K31/445 ,  C07D401/06 ,  C07D403/06

CPC分类号： C07D231/12 ,  C07D233/56 ,  C07D249/08

摘要： There is disclosed a fungicide containing, effective ingredient, a compound having the general formula (I): ##STR1## or an acid addition salt thereof, particularly the compound wherein an absolute configuration of the asymmetric carbon atoms is R,R-configuration or an acid addition salt thereof.

摘要翻译： 公开了含有有效成分的具有通式（I）的化合物或其酸加成盐的杀真菌剂，特别是其中不对称碳原子的绝对构型为R的化合物，R- 构型或其酸加成盐。

公开(公告)号：US5115379A

公开(公告)日：1992-05-19

申请号：US635795

申请日：1990-12-31

申请人： Kiyoshi Nagai

发明人： Kiyoshi Nagai

IPC分类号： G01D11/28 ,  G12B11/00 ,  G12B11/02

CPC分类号： G01D11/28 ,  G12B11/00

摘要： A composite filter advantageously employable for dark field observation systems which comprises a dyed organic resin layer provided on a dyed glass plate. The organic resin layer and the glass plate in combination absorb light of a radiation spectrum in the region of longer than 600 nm under the condition that the transmission of light at 650 nm is defined to a level of 1.times.10.sup.-4 % to 5% and the transmission of light at 800 nm is defined to a level of lower than 2.times.10.sup.-5 %. Another dyed glass plate which shows an absorption spectrum having absorption peaks in regions of 480-535 nm and 560-600 nm may be incorporated into the composite filter. The composite filter can be introduced into or provided to an illuminating device.

摘要翻译： 有利地用于暗场观察系统的复合过滤器，其包括设在染色玻璃板上的染色有机树脂层。 有机树脂层和玻璃板在650nm以上的光的透过率定义为1×10 -4％〜5％的水平的条件下，组合吸收了长于600nm以上的区域的辐射光谱， 在800nm处的光的透射被定义为低于2×10-5％的水平。 另外，在480-535nm和560-600nm的区域显示出具有吸收峰的吸收光谱的染色玻璃板可以并入到复合滤光器中。 复合过滤器可被引入照明装置或提供给照明装置。

公开(公告)号：US4471206A

公开(公告)日：1984-09-11

申请号：US426456

申请日：1982-09-29

申请人： Kiyoshi Nagai

发明人： Kiyoshi Nagai

IPC分类号： A47J41/02 ,  B23K15/00 ,  B23K15/06

CPC分类号： A47J41/028 ,  B23K15/06

摘要： A method of evacuating and sealing the vacuum chamber of a stainless steel vacuum bottle includes the steps of piercing the outer bottle with only very small openings into the vacuum chamber and closing the openings by means of an electron-beam welder after the bottle is heated and the vacuum chamber evacuated.

摘要翻译： 一种对不锈钢真空瓶的真空室进行排空和密封的方法包括以下步骤：将仅具有非常小的开口的外部瓶子刺入真空室中，并且在加热瓶子之后通过电子束焊机封闭开口; 真空室抽真空。