公开(公告)号：US20060024841A1

公开(公告)日：2006-02-02

申请号：US11243113

申请日：2005-10-04

申请人： Xian-Wei Yao ,  Chao Lin ,  Paul Heaney ,  Thomas Becker ,  Aaron Hanson ,  Michael Willis

发明人： Xian-Wei Yao ,  Chao Lin ,  Paul Heaney ,  Thomas Becker ,  Aaron Hanson ,  Michael Willis

IPC分类号： G01N1/10 ,  G01D15/04

CPC分类号： B01J19/0046 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/0244 ,  B01L3/0248 ,  B01L2200/0657 ,  B82Y30/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N1/10 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/10 ,  G01N35/1016 ,  G01N35/1065 ,  G01N2035/00158 ,  G01N2035/1037 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/119163 ,  Y10T436/24 ,  Y10T436/2575

摘要： A slotted pin tool, a delivery system containing the pin tool, a substrate for use in the system and methods using the pin tool and system are provided. The slotted pin tool contains a plurality of pins having slotted ends designed to fit around each loci of material deposited on a surface, such as a microarray, without contacting any of the deposited material. Sample is delivered by contacting the pin tool with the surface; the amount delivered is proportional to the velocity of the pin tool as it contacts the surface or the velocity of the liquid when movement of the pin is halted.

公开(公告)号：US5958202A

公开(公告)日：1999-09-28

申请号：US792933

申请日：1997-01-22

申请人： Fred E. Regnier ,  Xian-Wei Yao ,  Todd A. Taylor ,  Martin Fuchs ,  Dieter Schmalzing ,  Lance Koutny ,  Wassim Nashabeh

发明人： Fred E. Regnier ,  Xian-Wei Yao ,  Todd A. Taylor ,  Martin Fuchs ,  Dieter Schmalzing ,  Lance Koutny ,  Wassim Nashabeh

IPC分类号： G01N33/561 ,  G01N27/447 ,  G01N33/537 ,  G01N33/543 ,  G01N33/78 ,  G01N27/26 ,  C12Q1/68 ,  G01N33/53

CPC分类号： G01N27/44747 ,  G01N27/44726 ,  G01N33/537 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/78

摘要： Disclosed are methods and apparatus for performing highly sensitive enzyme-amplified assays using channel electrophoresis of an enzyme having a biorecognition moiety. Combining a binding reaction of an analyte and an enzyme having a biorecognition moiety, and an electrophoretic separation which uses enzyme-amplified detection methodology, assay methods and apparatus are provided that offer simplicity, quantitative sensitivity and rapid analysis. Also disclosed are highly sensitive catalytically-amplified assays using a capillary electrophoresis format in which the binding reaction prior to electrophoresis is heterogeneous or homogeneous, and direct or competitive. The methods and apparatus for conducting sensitive automated catalytically-amplified assays using electrophoresis may be conducted in a microscale format.

摘要翻译： 公开了使用具有生物识别部分的酶的通道电泳进行高度灵敏的酶扩增测定的方法和装置。 结合分析物和具有生物识别部分的酶的结合反应以及使用酶扩增检测方法的测定方法和装置，提供了简单性，定量灵敏度和快速分析的电泳分离。 还公开了使用毛细管电泳方式的高度敏感的催化扩增测定法，其中电泳前的结合反应是异质或均相的，直接的或竞争性的。 使用电泳进行敏感的自动化催化扩增测定的方法和装置可以以微型格式进行。

公开(公告)号：US06368562B1

公开(公告)日：2002-04-09

申请号：US09292868

申请日：1999-04-16

申请人： Xian-Wei Yao

发明人： Xian-Wei Yao

IPC分类号： B01L300

CPC分类号： B01L3/0268 ,  B01F5/0604 ,  B01F13/0059 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00353 ,  B01J2219/00585 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00704 ,  B01J2219/00707 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502738 ,  B01L2300/0829 ,  B01L2400/027 ,  B01L2400/0406 ,  B01L2400/0487 ,  H01J49/04

摘要： A method and apparatus for fluid transportation includes a fluid reservoir and a pump that supplies fluid to a microfluidic device. The microfluidic device has an opening and an electrode positioned proximate the opening. The pump pressurizes fluid within the microfluidic device to form a droplet at the opening. When a desired volume of droplet is formed, a potential difference is generated between an electrode and a target plate. The potential difference causes the drop to form a fluid delivery therebetween. The fluid delivery may take many shapes, including a Taylor cone or a stream of droplets.

摘要翻译： 用于流体输送的方法和装置包括将流体供应到微流体装置的流体储存器和泵。 微流体装置具有开口和位于开口附近的电极。 泵对微流体装置内的流体加压，以在开口处形成液滴。 当形成所需体积的液滴时，在电极和靶板之间产生电位差。 电位差导致液滴在其间形成流体输送。 流体输送可以采取许多形状，包括泰勒锥或液滴流。

公开(公告)号：US20050053944A1

公开(公告)日：2005-03-10

申请号：US10636343

申请日：2003-08-07

申请人： Martin Fuchs ,  Michael Egholm ,  Heather O'Keefe ,  Xian-Wei Yao

发明人： Martin Fuchs ,  Michael Egholm ,  Heather O'Keefe ,  Xian-Wei Yao

IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6813 ,  G01N27/447 ,  G01N33/566 ,  C07K14/47

CPC分类号： G01N27/44726 ,  C12Q1/6813 ,  G01N27/447 ,  C12Q2525/113 ,  C12Q2523/113 ,  C12Q2565/125 ,  C12Q2565/629 ,  C12Q2565/107

摘要： A method composition and apparatus for the hybridization and separation of molecules having a desired target sequence in a sample by contacting a sample of single stranded nucleic acids with a detectable PNA probe having a sequence complementary to the target sequence so that the target sequence, if present, will hybridize with the detectable probe to form a detectable duplex, and then separating the duplex in a denaturing medium from unbound sample components by electrophoresis. The invention also relates to methods compositions and apparatus for the hybridization and separation of molecules having a desired target sequence in a mixed sample of single stranded nucleic acids and their complementary strands by contacting the sample with a detectable PNA probe.

摘要翻译： 一种方法组合物和装置，用于通过使单链核酸样品与具有与靶序列互补的序列的可检测PNA探针接触来杂交和分离样品中具有所需靶序列的分子，使得靶序列（如果存在） 将与可检测的探针杂交以形成可检测的双链体，然后通过电泳将变性培养基中的双链体与未结合的样品组分分离。 本发明还涉及用于通过使样品与可检测的PNA探针接触来在单链核酸及其互补链的混合样品中杂交和分离具有所需靶序列的分子的方法组合物和装置。

公开(公告)号：US06605475B1

公开(公告)日：2003-08-12

申请号：US09293314

申请日：1999-04-16

申请人： Todd A. Taylor ,  William W. Carson ,  Xian-Wei Yao

发明人： Todd A. Taylor ,  William W. Carson ,  Xian-Wei Yao

IPC分类号： G01N110

CPC分类号： G01N27/44791 ,  G01N27/44743 ,  Y10T436/2575

摘要： Apparatus and methods have been developed to deliver automatically a sample to a reaction vessel, an analytical device or any location where sample introduction or deposition is desired. A sample delivery system of the invention generally includes a housing defining a channel, e.g., a capillary, and a volume controller, which is a temperature control device, in thermal communication with the channel. The channel preferably is closed at one end, and contains an opening for introduction of a sample. The closed end of the channel is associated thermally with the temperature control device. The temperature control device heats and cools a thermally expandable fluid in the channel which controls movement of a sample into, within and out of the channel.

摘要翻译： 已经开发了装置和方法，以将样品自动递送到反应容器，分析装置或需要样品引入或沉积的任何位置。 本发明的样品输送系统通常包括限定通道的壳体，例如毛细管，以及与通道热连通的温度控制装置的体积控制器。 通道优选在一端封闭，并且包含用于引入样品的开口。 通道的封闭端与温度控制装置热连接。 温度控制装置加热和冷却通道中的热膨胀流体，其控制样品进入通道内和外通道的运动。