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    Fluidics platform and method for sample preparation and analysis
    1.
    发明授权
    Fluidics platform and method for sample preparation and analysis 有权
    流体平台和样品制备和分析方法

    公开(公告)号：US08808643B1

    公开(公告)日：2014-08-19

    申请号：US13228391

    申请日：2011-09-08

    申请人： W. Henry Benner ,  John M. Dzenitis ,  William J Benett ,  Brian R. Baker

    发明人： W. Henry Benner ,  John M. Dzenitis ,  William J Benett ,  Brian R. Baker

    IPC分类号： B01L3/02

    CPC分类号： B01L3/502715 ,  B01L3/502738 ,  B01L2300/0864 ,  B01L2400/0622

    摘要： Herein provided are fluidics platform and method for sample preparation and analysis. The fluidics platform is capable of analyzing DNA from blood samples using amplification assays such as polymerase-chain-reaction assays and loop-mediated-isothermal-amplification assays. The fluidics platform can also be used for other types of assays and analyzes. In some embodiments, a sample in a sealed tube can be inserted directly. The following isolation, detection, and analyzes can be performed without a user's intervention. The disclosed platform may also comprises a sample preparation system with a magnetic actuator, a heater, and an air-drying mechanism, and fluid manipulation processes for extraction, washing, elution, assay assembly, assay detection, and cleaning after reactions and between samples.

    摘要翻译： 本文提供了流动平台和样品制备和分析方法。 流体学平台能够使用诸如聚合酶链反应测定和环介导等温扩增测定之类的扩增测定法从血液样品中分析DNA。 流体学平台也可用于其他类型的测定和分析。 在一些实施例中，密封管中的样品可以直接插入。 无需用户干预即可执行以下隔离，检测和分析。 所公开的平台还可以包括具有磁性致动器，加热器和空气干燥机构的样品制备系统，以及用于在反应之后和样品之间的萃取，洗涤，洗脱，测定组装，测定检测和清洁的流体操纵过程。

    Fluidics platform and method for sample preparation
    2.
    发明授权
    Fluidics platform and method for sample preparation 有权
    流体平台和样品制备方法

    公开(公告)号：US09372135B1

    公开(公告)日：2016-06-21

    申请号：US13228370

    申请日：2011-09-08

    申请人： W. Henry Benner ,  John M. Dzenitis

    发明人： W. Henry Benner ,  John M. Dzenitis

    IPC分类号： G01N1/00 ,  B01L3/00 ,  G01N1/40 ,  G01N35/00

    CPC分类号： G01N1/405 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2400/0475 ,  B01L2400/0478 ,  B01L2400/0605 ,  G01N1/286 ,  G01N1/40 ,  G01N1/44 ,  G01N35/0098

    摘要： Provided herein are fluidics platforms and related methods for performing integrated sample collection and solid-phase extraction of a target component of the sample all in one tube. The fluidics platform comprises a pump, particles for solid-phase extraction and a particle-holding means. The method comprises contacting the sample with one or more reagents in a pump, coupling a particle-holding means to the pump and expelling the waste out of the pump while the particle-holding means retains the particles inside the pump. The fluidics platform and methods herein described allow solid-phase extraction without pipetting and centrifugation.

    摘要翻译： 本文提供了流体平台和相关方法，用于在一个管中进行样品收集和样品的目标组分的固相萃取。 流体平台包括泵，用于固相萃取的颗粒和颗粒保持装置。 该方法包括使样品与泵中的一种或多种试剂接触，将颗粒保持装置耦合到泵并将废物排出泵，同时颗粒保持装置将颗粒保持在泵内。 本文所述的流体学平台和方法允许固相萃取而不需要移液和离心。

    ION MOBILITY SPECTROMETER WITH CENTER ROD
    3.
    发明申请
    ION MOBILITY SPECTROMETER WITH CENTER ROD 有权

    公开(公告)号：US20210278369A1

    公开(公告)日：2021-09-09

    申请号：US17259081

    申请日：2019-07-10

    申请人： W. Henry Benner ,  Michael J. Bogan ,  Ben Aguilar

    发明人： W. Henry Benner ,  Michael J. Bogan ,  Ben Aguilar

    IPC分类号： G01N27/622

    摘要： Apparatuses and methods are described for determining properties of ions travelling through a gas under the influence of an electric field. The apparatuses and methods can be understood to provide measurements of the electrical mobility of ions as useful for determining the electrical mobility constant Ko of electrosprayed substances, such as proteins. The apparatuses and methods relate to the scientific discipline of ion mobility spectrometry. Modules connected to ion mobility spectrometers provide stress to substances for the purpose of investigating, for example, the thermal stability of proteins. One form of the technology includes a tubular spectrometer body having an electrically conductive inner wall; a rod positioned along the longitudinal center of the body and electrodes positioned on, but electrically isolated from, the inner wall, where the ratio of the radius of the tubular spectrometer body to the ratio of the radius of the rod is at least 20.

    BIOLOGICAL PARTICLES
    5.
    发明申请
    BIOLOGICAL PARTICLES 有权
    生物颗粒

    公开(公告)号：US20110089037A1

    公开(公告)日：2011-04-21

    申请号：US12966739

    申请日：2010-12-13

    申请人： W. Henry Benner ,  Ronald M. Krauss ,  Patricia J. Blanche

    发明人： W. Henry Benner ,  Ronald M. Krauss ,  Patricia J. Blanche

    IPC分类号： G01N27/447 ,  C07K14/775 ,  C07K14/005 ,  C07K16/00

    CPC分类号： G01N15/0266 ,  G01N33/92 ,  G01N2800/52 ,  Y10T436/104165 ,  Y10T436/24

    摘要： A medical diagnostic method and instrumentation system for analyzing noncovalently bonded agglomerated biological particles is described. The method and system comprises: a method of preparation for the biological particles; an electrospray generator; an alpha particle radiation source; a differential mobility analyzer; a particle counter; and data acquisition and analysis means. The medical device is useful for the assessment of human diseases, such as cardiac disease risk and hyperlipidemia, by rapid quantitative analysis of lipoprotein fraction densities. Initially, purification procedures are described to reduce an initial blood sample to an analytical input to the instrument. The measured sizes from the analytical sample are correlated with densities, resulting in a spectrum of lipoprotein densities. The lipoprotein density distribution can then be used to characterize cardiac and other lipid-related health risks.

    摘要翻译： 描述了用于分析非共价结合的附聚生物颗粒的医学诊断方法和仪器系统。 该方法和系统包括：一种制备生物颗粒的方法; 电喷发生器; α粒子辐射源; 差分迁移率分析仪; 粒子计数器 数据采集​​和分析手段。 通过脂蛋白部分密度的快速定量分析，医疗装置可用于评估人类疾病，如心脏病风险和高脂血症。 最初，描述了纯化程序以将初始血液样品减少到仪器的分析输入。 来自分析样品的测量尺寸与密度相关，导致脂蛋白密度谱。 然后可以将脂蛋白密度分布用于表征心脏和其他与脂质有关的健康风险。

    Ion mobility analysis of lipoproteins
    6.
    发明授权
    Ion mobility analysis of lipoproteins 有权
    脂蛋白的离子迁移率分析

    公开(公告)号：US07259018B2

    公开(公告)日：2007-08-21

    申请号：US10293610

    申请日：2002-11-12

    申请人： W. Henry Benner ,  Ronald M. Krauss ,  Patricia J. Blanche

    发明人： W. Henry Benner ,  Ronald M. Krauss ,  Patricia J. Blanche

    IPC分类号： G01N33/92 ,  G01N24/00

    CPC分类号： G01N15/0266 ,  G01N33/92 ,  G01N2800/52 ,  Y10T436/104165 ,  Y10T436/24

    摘要： A medical diagnostic method and instrumentation system for analyzing noncovalently bonded agglomerated biological particles is described. The method and system comprises: a method of preparation for the biological particles; an electrospray generator; an alpha particle radiation source; a differential mobility analyzer; a particle counter; and data acquisition and analysis means. The medical device is useful for the assessment of human diseases, such as cardiac disease risk and hyperlipidemia, by rapid quantitative analysis of lipoprotein fraction densities. Initially, purification procedures are described to reduce an initial blood sample to an analytical input to the instrument. The measured sizes from the analytical sample are correlated with densities, resulting in a spectrum of lipoprotein densities. The lipoprotein density distribution can then be used to characterize cardiac and other lipid-related health risks.

    摘要翻译： 描述了用于分析非共价结合的附聚生物颗粒的医学诊断方法和仪器系统。 该方法和系统包括：一种制备生物颗粒的方法; 电喷发生器; α粒子辐射源; 差分迁移率分析仪; 粒子计数器 数据采集​​和分析手段。 通过脂蛋白部分密度的快速定量分析，医疗装置可用于评估人类疾病，如心脏病风险和高脂血症。 最初，描述了纯化程序以将初始血液样品减少到仪器的分析输入。 来自分析样品的测量尺寸与密度相关，导致脂蛋白密度谱。 然后可以将脂蛋白密度分布用于表征心脏和其他与脂质有关的健康风险。

    Apparatus and method of determining molecular weight of large molecules
    8.
    发明授权
    Apparatus and method of determining molecular weight of large molecules 失效
    测定大分子量分子量的仪器和方法

    公开(公告)号：US5770857A

    公开(公告)日：1998-06-23

    申请号：US749837

    申请日：1996-11-15

    申请人： Stephen Fuerstenau ,  W. Henry Benner ,  Norman Madden ,  William Searles

    发明人： Stephen Fuerstenau ,  W. Henry Benner ,  Norman Madden ,  William Searles

    IPC分类号： H01J49/04 ,  H01J37/244

    CPC分类号： H01J49/165 ,  H01J49/027

    摘要： A mass spectrometer determines the mass of multiply charged high molecular weight molecules. This spectrometer utilizes an ion detector which is capable of simultaneously measuring the charge z and transit time of a single ion as it passes through the detector. From this transit time, the velocity of the single ion may then be derived, thus providing the mass-to-charge ratio m/z for a single ion which has been accelerated through a known potential. Given z and m/z, the mass m of the single ion can then be calculated. Electrospray ions with masses in excess of 1 MDa and charge numbers greater than 425 e.sup.- are readily detected. The on-axis single ion detection configuration enables a duty cycle of nearly 100% and extends the practical application of electrospray mass spectrometry to the analysis of very large molecules with relatively inexpensive instrumentation.

    摘要翻译： 质谱仪确定多重带电的高分子量分子的质量。 该光谱仪使用离子检测器，其能够同时测量单个离子通过检测器时的电荷z和通过时间。 从该通过时间开始，可以导出单个离子的速度，从而为通过已知电位加速的单个离子提供质荷比m / z。 给定z和m / z，则可以计算单一离子的质量m。 质量超过1 MDa且电荷数大于425 e-的电喷雾离子很容易被检测到。 轴上单离子检测配置可实现近100％的占空比，并将电喷雾质谱的实际应用扩展到具有相对便宜的仪器的非常大的分子分析。