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    Nucleic acids encoding fusion peptides
    7.
    发明授权
    Nucleic acids encoding fusion peptides 有权
    编码融合肽的核酸

    公开(公告)号：US07348423B2

    公开(公告)日：2008-03-25

    申请号：US10969624

    申请日：2004-10-20

    申请人： Eva Hoess ,  Thomas Meier ,  Gabriele Pestlin ,  Friedrich Popp ,  Klaus Reichert ,  Rainer Schmuck ,  Bernd Schneidinger ,  Christoph Seidel ,  Wilhelm Tischer

    发明人： Eva Hoess ,  Thomas Meier ,  Gabriele Pestlin ,  Friedrich Popp ,  Klaus Reichert ,  Rainer Schmuck ,  Bernd Schneidinger ,  Christoph Seidel ,  Wilhelm Tischer

    IPC分类号： A61K39/21

    CPC分类号： C07K14/005 ,  C07K2319/00 ,  C12N2740/15022 ,  C12N2740/16122 ,  C12P21/02

    摘要： A process is disclosed for the production of an antifusogenic peptide by producing a fusion peptide of a length of about 14 to 70 amino acids in a prokaryotic host cell, comprising the steps, under such conditions that inclusion bodies of said fusion peptide are formed, of: (a) expressing in said host cell a nucleic acid encoding said fusion peptide consisting of a first peptide which is an antifusogenic peptide of a length of about 10 to 50 amino acids and a second peptide of a length of about 4 to 30 amino acids, said first peptide being N-terminally linked to said second peptide; (b) cultivating said host cell to produce said inclusion bodies; and (c) recovering said antifusogenic peptide from said inclusion bodies, wherein said recovered antifusogenic peptide consists of said fusion peptide or a peptide comprising the antifusogenic peptide of about 10 to 50 amino acids and which is a fragment cleaved from said fusion peptide. Inclusion bodies of the peptides are disclosed. Also disclosed is a nucleic acid encoding the fusion peptide consisting of a first peptide which is an antifusogenic peptide selected from the group of SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, and said sequences further consisting of glycine at the C terminal end, N-terminally linked to the second peptide.

    摘要翻译： 公开了通过在原核宿主细胞中产生约14至70个氨基酸长度的融合肽来生产抗融合肽的方法，包括以下步骤：在形成所述融合肽的包涵体的条件下， （a）在所述宿主细胞中表达编码所述融合肽的核酸，所述融合肽由长度为约10至50个氨基酸的抗融合肽的第一肽和长度为约4-30个氨基酸的第二个肽组成 所述第一肽与所述第二肽N-末端连接; （b）培养所述宿主细胞以产生所述包涵体; 和（c）从所述包涵体中回收所述抗融合肽，其中所述回收的抗融合肽由所述融合肽或包含约10至50个氨基酸的抗融合肽的肽组成，并且是从所述融合肽切割的片段。 公开了肽的包涵体。 还公开了编码由第一肽组成的融合肽的核酸，第一肽是选自SEQ ID NO：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10的抗融合肽， 所述序列进一步由C末端的甘氨酸组成，N-末端与第二肽连接。