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    Direct differentiation method for making cardiomyocytes from human emberyonic stem cells
    2.
    发明申请
    Direct differentiation method for making cardiomyocytes from human emberyonic stem cells 有权
    直接分化方法用于制造人类胚胎干细胞的心肌细胞

    公开(公告)号：US20050214939A1

    公开(公告)日：2005-09-29

    申请号：US11086709

    申请日：2005-03-21

    申请人： Joseph Gold ,  Mohammad Hassanipour ,  Lila Collins ,  Chunhui Xu

    发明人： Joseph Gold ,  Mohammad Hassanipour ,  Lila Collins ,  Chunhui Xu

    IPC分类号： C12N5/077 ,  C12N5/08

    CPC分类号： C12N5/0657 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2506/02

    摘要： This invention provides a new procedure for generating cardiomyocyte lineage cells from embryonic stem cells for use in regenerative medicine. Differentiating by way of embryoid body formation or in serum is no longer required. Instead, the stem cells are plated onto a solid substrate, and differentiated in the presence of select factors and morphogens. After enrichment for cells with the appropriate phenotype, the cells are allowed to cluster into cardiac bodies™, which are remarkably homogeneous and suitable for the treatment of heart disease.

    摘要翻译： 本发明提供了用于从再生医学中使用的胚胎干细胞产生心肌细胞谱系细胞的新方法。 不再需要通过胚状体形成或血清分化。 相反，将干细胞铺在固体底物上，并在存在选择因子和形态发生的情况下分化。 在具有适当表型的细胞富集后，允许细胞聚集到心脏体（TM）中，其是非常均匀的并且适合于治疗心脏病。

    Cardiac bodies: clusters of spontaneously contracting cells for regenerating cardiac function
    6.
    发明申请
    Cardiac bodies: clusters of spontaneously contracting cells for regenerating cardiac function 审中-公开
    心脏体：用于再生心脏功能的自发收缩细胞簇

    公开(公告)号：US20050214938A1

    公开(公告)日：2005-09-29

    申请号：US11085899

    申请日：2005-03-21

    申请人： Joseph Gold ,  Chunhui Xu

    发明人： Joseph Gold ,  Chunhui Xu

    IPC分类号： C12N5/077 ,  C12N5/08

    CPC分类号： C12N5/0657 ,  C12N2500/33 ,  C12N2501/33 ,  C12N2506/02 ,  C12N2533/50 ,  C12N2533/90

    摘要： This disclosure describes clusters of cardiomyocyte lineage cells referred to as cardiac bodies. They can be obtained by differentiating human embryonic stem cells into cells that express cardiomyocyte markers, and separating cells according to their density. Single suspended cells are removed, leaving self-aggregating clusters that can be propagated and enriched in further separation steps. The resulting cardiac bodies express cardiomyocyte markers at levels ˜100-fold above the starting cell population, and undergo spontaneous periodic contraction. The clusters can be used intact or dispersed into single-cell suspensions for use in research, drug screening or the preparation of pharmaceutical compositions for the treatment of cardiac disease.

    摘要翻译： 本公开描述了称为心脏体的心肌细胞谱系细胞簇。 它们可以通过将人胚胎干细胞分化成表达心肌细胞标记的细胞，并根据其密度分离细胞来获得。 去除单个悬浮细胞，留下可以进一步分离步骤繁殖和富集的自聚集簇。 所产生的心脏体表达高于起始细胞群高100倍的心肌细胞标志物，并经历自发性周期性收缩。 这些簇可以完整使用或分散在单细胞悬浮液中，用于研究，药物筛选或用于治疗心脏病的药物组合物的制备。