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    Monolith reactor containing a plurality of flow passages and method for carrying out biological reactions
    1.
    发明授权
    Monolith reactor containing a plurality of flow passages and method for carrying out biological reactions 失效
    包含多个流动通道的整体反应器和进行生物反应的方法

    公开(公告)号：US4948728A

    公开(公告)日：1990-08-14

    申请号：US166258

    申请日：1988-03-10

    申请人： Gregory Stephanopoulos ,  Julia A. Kornfield ,  Gerald E. Voecks

    发明人： Gregory Stephanopoulos ,  Julia A. Kornfield ,  Gerald E. Voecks

    IPC分类号： C12M1/40

    CPC分类号： C12M47/10 ,  C12M23/20 ,  C12M23/24 ,  C12M29/04 ,  Y10S435/813 ,  Y10S435/818

    摘要： Biological reactions are carried out with a unitary structure, preferably formed of a ceramic material, having a plurality of flow passages including first and second sets of flow passages arranged so that individual passages of the first set are adjacent individual passages of the second set and are separated by walls formed of the ceramic material. The ceramic material is porous to provide mass transfer of gaseous oxygen and biological reaction products while containing liquid in the second set of passages. Walls of the passages may be covered with a gaseous oxygen permeable membrane. Inside walls of the second set of passages may coated with a compound adapted to immobilize biological reaction materials. In a biological reaction, the first set of passages are contacted with a fluid such as an air stream to provide gaseous oxygen, and a fluid flow such as a nutrient medium is established through the second set of flow passages whereby an oxygen flow producing gradient is produced through the porous ceramic material between the first and second sets of flow passages to supply oxygen for cells immobilized on inside walls of the second set of flow passages.

    摘要翻译： 生物反应采用整体结构进行，优选由陶瓷材料形成，其具有多个流动通道，其包括第一组和第二组流动通道，其布置成使得第一组的各个通道相邻于第二组的各个通道，并且分别是 由陶瓷材料形成的壁分隔开。 陶瓷材料是多孔的，以提供气态氧和生物反应产物的质量传递，同时在第二组通道中含有液体。 通道的壁可以用气体氧气渗透膜覆盖。 第二组通道的内壁可涂覆有适于固定生物反应材料的化合物。 在生物反应中，第一组通道与诸如空气流的流体接触以提供气态氧，并且通过第二组流动通道建立流体流如营养培养基，由此产生氧气的梯度为 通过第一组和第二组流动通道之间的多孔陶瓷材料产生，以便为固定在第二组流动通道的内壁上的细胞供应氧气。

    Methods and systems for generating and evaluating peptides
    6.
    发明授权
    Methods and systems for generating and evaluating peptides 失效
    用于产生和评估肽的方法和系统

    公开(公告)号：US07739055B2

    公开(公告)日：2010-06-15

    申请号：US11561266

    申请日：2006-11-17

    申请人： Gregory Stephanopoulos ,  Kyle Jensen ,  Christopher Loose

    发明人： Gregory Stephanopoulos ,  Kyle Jensen ,  Christopher Loose

    IPC分类号： G06F19/00

    CPC分类号： C07K7/08 ,  A61K38/00 ,  C07K14/001 ,  C07K14/4723 ,  C40B50/02 ,  G06F19/18 ,  G06F19/22 ,  G06F19/24 ,  G06F19/28

    摘要： Methods to create databases of peptides having a desirable property, such as antimicrobial activity, involving analyzing a database of known peptides for a pattern statistically associated with the desirable property are described herein, The set of sequences being analyzed may include sequences of a desired length containing all or substantially all combinations of amino acids that conform to at least one of the set of patterns. Once the database is identified, the database may be processed in a pattern recognition procedure that identifies a set of patterns that may be representative of a peptide having the desirable property. A set of newly generated peptides sequences may then be processed to score these new sequences against the identified patterns to correlate the patterns to the sequences and determine a degree of association or similarity between one or more of the new sequences and the set of identified patterns.

    摘要翻译： 本文描述了创建具有期望性质（例如抗微生物活性）的肽的数据库的方法，其涉及分析已知肽的数据库以获得与所需性质统计学相关的模式。所分析的一组序列可以包含期望长度的序列，其含有 所有或基本上所有符合该组图案中的至少一个的氨基酸组合。 一旦识别了数据库，就可以在模式识别程序中处理数据库，该模式识别程序识别可以代表具有所需性质的肽的一组模式。 然后可以处理一组新产生的肽序列以针对所识别的模式对这些新序列进行评估，以将模式与序列相关联，并确定一个或多个新序列与所识别的模式集合之间的关联度或相似度。

    MICROFLUIDIC DROPLETS FOR METABOLIC ENGINEERING AND OTHER APPLICATIONS
    7.
    发明申请
    MICROFLUIDIC DROPLETS FOR METABOLIC ENGINEERING AND OTHER APPLICATIONS 审中-公开
    用于代谢工程和其他应用的微流控溅射

    公开(公告)号：US20100124759A1

    公开(公告)日：2010-05-20

    申请号：US12492830

    申请日：2009-06-26

    申请人： Benjamin L. Wang ,  Katherine J. Humphry ,  David A. Weitz ,  Gregory Stephanopoulos

    发明人： Benjamin L. Wang ,  Katherine J. Humphry ,  David A. Weitz ,  Gregory Stephanopoulos

    IPC分类号： C12Q1/28 ,  C12N1/18 ,  C12Q1/26 ,  C12N1/20

    CPC分类号： B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421

    摘要： The present invention relates generally to the use of droplets to culture and/or assay cells or other species. In some cases, the cells or other species may be sorted based upon the results of the culture and/or assay. In some embodiments, cells other species can be encapsulated in droplets and exposed to one or more agents (e.g., a sugar, an indicator dye, etc.). For instance, in some cases, exposure of cells to the agents may result in the production of metabolites or other compounds (e.g., amino acids, proteins, organic acids, etc.) which may be, for example, assayed or otherwise determined. In some embodiments, the reaction of an agent with cells and/or other species within a droplet may reveal a property of the cells or other species (e.g., sugar consumption, growth rate, ability to withstand exposure to the agent, etc.). As an example, cells that produce desired metabolites or exhibit certain properties may be separated from the other cells via sorting techniques. Other aspects of the invention relate to devices or kits for implementing such sorts, methods of promoting such techniques, or the like.

    摘要翻译： 本发明一般涉及使用液滴来培养和/或测定细胞或其它物种。 在一些情况下，可以基于培养和/或测定的结果对细胞或其它物种进行分类。 在一些实施方案中，细胞其他物质可以被包封在液滴中并暴露于一种或多种试剂（例如，糖，指示剂染料等）。 例如，在一些情况下，细胞暴露于试剂可导致生成代谢物或其它化合物（例如，氨基酸，蛋白质，有机酸等），其可以例如被测定或以其他方式测定。 在一些实施方案中，药剂与细胞和/或液滴内的其它物质的反应可以揭示细胞或其他物种的性质（例如，糖消耗，生长速率，耐受试剂暴露的能力等）。 作为实例，产生所需代谢物或显示某些特性的细胞可以通过分选技术与其他细胞分离。 本发明的其他方面涉及用于实现这种类型的装置或套件，促进这种技术的方法等。