公开(公告)号：US4637982A

公开(公告)日：1987-01-20

申请号：US527579

申请日：1983-07-05

申请人： Hideaki Yamada ,  Yoshiki Tani

发明人： Hideaki Yamada ,  Yoshiki Tani

IPC分类号： C12P13/02 ,  C12R1/01 ,  C12R1/38 ,  C12N9/78 ,  C12P1/04

CPC分类号： C12P13/02 ,  Y10S435/874

摘要： In a process for hydrating a nitrile having 2 to 4 carbon atoms by a microbiological treatment to convert the nitrile into the corresponding amide, it is possible to biologically prepare the corresponding amide effectively by utilizing a microorganism of the genus Pseudomonas which is capable of hydrating the nitrile to convert the same into the corresponding amide. In particular, it is possible to obtain acrylamides which are highly useful polymers by the very simple and energy-saving process in accordance with the present invention.

摘要翻译： PCT No.PCT / JP82 / 00393 Sec。 371日期：1983年7月5日 102（e）日期1983年7月5日PCT提交1982年9月29日PCT公布。 公开号WO83 / 01784 日期：1983年5月26日。在通过微生物处理将腈转化成相应的酰胺的情况下，使具有2〜4个碳原子的腈水合的方法中，可以通过利用假单胞菌属的微生物有效地制备相应的酰胺 其能够使腈水合以将其转化为相应的酰胺。 特别地，可以通过根据本发明的非常简单和节能的方法获得高度有用的聚合物的丙烯酰胺。

公开(公告)号：US4229538A

公开(公告)日：1980-10-21

申请号：US28604

申请日：1979-04-09

申请人： Hideaki Yamada ,  Sakayu Shimizu ,  Yoshiki Tani

发明人： Hideaki Yamada ,  Sakayu Shimizu ,  Yoshiki Tani

IPC分类号： C12N9/00 ,  C12Q1/25 ,  C12N9/10

CPC分类号： C12N9/93 ,  C12Q1/25 ,  Y10S435/875 ,  Y10S435/911

摘要： Acyl-CoA synthetase, having a high activity to C.sub.16 -C.sub.18 long chain fatty acids, is obtained by culturing strains belonging to various genera. As is known, acyl-CoA synthetase, having a strong activity to C.sub.16 -C.sub.18 long chain fatty acids, is generally obtained from liver of rat; however, it has now been discovered that acyl-CoA synthetase can be obtained from microorganisms and this enzyme is called acyl-CoA synthetase LCF-18. By use of acyl-CoA synthetase LCF-18 of the present invention which has a high activity to C.sub.16 -C.sub.18 long chain fatty acids, serum non-esterified fatty acid of human beings can be accurately determined, and it is very useful for diagnosis of diabetes and so forth.

摘要翻译： 通过培养属于各种属的菌株获得对C16-C18长链脂肪酸具有高活性的酰基辅酶A合成酶。 众所周知，对C16-C18长链脂肪酸具有强烈活性的酰基辅酶A合成酶通常由大鼠肝脏获得; 然而，现在已经发现可以从微生物获得酰基辅酶A合成酶，该酶称为酰基辅酶A合成酶LCF-18。 通过使用对C16-C18长链脂肪酸具有高活性的本发明的酰基-CoA合成酶LCF-18，可以准确测定人的血清非酯化脂肪酸，并且其对于诊断 糖尿病等。

公开(公告)号：US5008188A

公开(公告)日：1991-04-16

申请号：US117439

申请日：1987-11-04

申请人： Hideaki Yamada ,  Yoshiki Tani ,  Sakayu Shimizu ,  Shozo Shiozaki

发明人： Hideaki Yamada ,  Yoshiki Tani ,  Sakayu Shimizu ,  Shozo Shiozaki

IPC分类号： C12P19/40 ,  C12R1/01 ,  C12R1/05 ,  C12R1/06 ,  C12R1/065 ,  C12R1/13 ,  C12R1/15 ,  C12R1/20 ,  C12R1/265 ,  C12R1/29 ,  C12R1/32 ,  C12R1/365 ,  C12R1/385 ,  C12R1/465 ,  C12R1/62 ,  C12R1/625 ,  C12R1/64 ,  C12R1/645 ,  C12R1/65 ,  C12R1/66 ,  C12R1/77 ,  C12R1/78 ,  C12R1/785 ,  C12R1/80 ,  C12R1/84 ,  C12R1/845 ,  C12R1/88 ,  C12P13/12 ,  C12P19/18

CPC分类号： C12P19/40

摘要： Process for producing S-adenosyl-L-homocysteine, which comprises reacting adenosine with homocysteine by contacting them in an aqueous medium in the presence of cells or treated cells of a microorganism of a specified genus having the ability to synthesize S-adenosyl-L-homocysteine from adenosine and homocysteine, and collecting the S-adenosyl-L-homocysteine synthesized.

摘要翻译： 该方法包括使腺苷与同型半胱氨酸反应，通过在具有合成S-腺苷-L-同型半胱氨酸的能力的特定属的微生物的细胞或处理细胞的存在下在水性介质中接触， 来自腺苷和高半胱氨酸的同型半胱氨酸，并收集合成的S-腺苷-L-高半胱氨酸。

公开(公告)号：US4563426A

公开(公告)日：1986-01-07

申请号：US505124

申请日：1983-06-21

申请人： Hideaki Yamada ,  Yoshiki Tani ,  Yoshikazu Izumi

发明人： Hideaki Yamada ,  Yoshiki Tani ,  Yoshikazu Izumi

IPC分类号： C12P13/00 ,  C12P17/18 ,  C12R1/01 ,  C12R1/07 ,  C12R1/38 ,  C12N1/38

CPC分类号： C12P17/186

摘要： In a process which comprises cultivating a micro-organism having the ability to produce a biotin-vitamer in a culture medium in the presence of a biotin-vitamer precursor thereby to produce the biotin-vitamer and accumulate it in the culture medium, the improvement wherein the biotin-vitamer precursor is added to the culture medium after the microbial cells have been grown.

摘要翻译： 在包括在生物素维生素前体的存在下培养在培养基中产生生物素维生素的能力的微生物的方法，从而产生生物素维生素并将其积累在培养基中，其改进在于 在培养微生物细胞后，将生物素维生素前体加入到培养基中。

公开(公告)号：US4360591A

公开(公告)日：1982-11-23

申请号：US212225

申请日：1980-12-02

申请人： Hideaki Yamada ,  Sakayu Shimizu ,  Yoshiki Tani ,  Kanzo Yamashita

发明人： Hideaki Yamada ,  Sakayu Shimizu ,  Yoshiki Tani ,  Kanzo Yamashita

IPC分类号： G01N33/50 ,  C12Q1/25 ,  C12Q1/44 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/48

CPC分类号： C12Q1/25

摘要： This invention relates to a process for the determination of free fatty acids comprising a reaction system 1 in which acyl coenzyme A synthetase is allowed to act on the free fatty acids in the presence of adenosine triphosphate and coenzyme A to yield acyl coenzyme A and a reaction system 2 in which acyl coenzyme A oxidase is allowed to act on the acyl coenzyme A to form hydrogen peroxide, followed by the determination thereof, said process being characterized in that myokinase is added in the reaction system 1 to make it go rapidly to completion.

摘要翻译： 本发明涉及一种测定游离脂肪酸的方法，其包括反应体系1，其中使酰基辅酶A合成酶在三磷酸腺苷和辅酶A存在下作用于游离脂肪酸，得到酰基辅酶A和反应 系统2，其中使酰基辅酶A氧化酶作用于酰基辅酶A以形成过氧化氢，随后进行测定，所述方法的特征在于将肌激蛋白酶加入反应体系1中使其快速完成。

公开(公告)号：US4373026A

公开(公告)日：1983-02-08

申请号：US216302

申请日：1980-12-12

申请人： Hideaki Yamada ,  Yoshiki Tani ,  Kimiyasu Isobe

发明人： Hideaki Yamada ,  Yoshiki Tani ,  Kimiyasu Isobe

IPC分类号： C12N9/06 ,  C12Q1/26 ,  C12R1/82 ,  C12P21/00

CPC分类号： C12N9/0026 ,  C12Q1/26 ,  Y10S435/935

摘要： A novel polyamine oxidase, polyamine oxidase PC-3, takes an important role participating in decomposition and metabolism and polyamines such as spermidine and spermine.

摘要翻译： 一种新型多胺氧化酶多胺氧化酶PC-3在分解代谢和多胺如亚精胺和精胺中起重要作用。

公开(公告)号：US4371621A

公开(公告)日：1983-02-01

申请号：US216301

申请日：1980-12-12

申请人： Hideaki Yamada ,  Yoshiki Tani ,  Kimiyasu Isobe

发明人： Hideaki Yamada ,  Yoshiki Tani ,  Kimiyasu Isobe

IPC分类号： C12N9/06 ,  C12R1/66 ,  C12R1/82

CPC分类号： C12N9/0026 ,  Y10S435/913

摘要： A novel polyamine oxidase, polyamine oxidase AT-1, takes an important role participating in decomposition and metabolism of polyamines such as spermidine and spermine.

摘要翻译： 一种新型多胺氧化酶多胺氧化酶AT-1在多胺如亚精胺和精胺的分解和代谢中起重要作用。

公开(公告)号：US4562149A

公开(公告)日：1985-12-31

申请号：US463990

申请日：1983-02-04

申请人： Shozo Shiozaki ,  Hideaki Yamada ,  Yoshiki Tani ,  Sakayu Shimizu

发明人： Shozo Shiozaki ,  Hideaki Yamada ,  Yoshiki Tani ,  Sakayu Shimizu

IPC分类号： C12N1/18 ,  C12P19/40 ,  C12P13/12 ,  C12N1/16 ,  C12P19/32

CPC分类号： C12P19/40 ,  C12N1/18 ,  Y10S435/94 ,  Y10S435/942

摘要： A yeast culture containing at least 10% by weight, based on the dry cell, of S-adenosyl methionine; and a process for producing S-adenosyl methionine, which comprises cultivating a yeast having the ability to produce S-adenosyl methionine in a liquid culture medium containing methionine to accumulate at least 10% by weight, based on the dry yeast cells, of S-adenosyl methionine in the yeast cells, separating the yeast cells from the culture medium, and thereafter obtaining S-adenosyl methionine in a stable form from the yeast cells.

摘要翻译： 含有至少10重量％的基于干细胞的S-腺苷甲硫氨酸的酵母培养物; 以及生产S-腺苷甲硫氨酸的方法，其包括在含有甲硫氨酸的液体培养基中培养具有产生S-腺苷甲硫氨酸能力的酵母以基于所述干酵母细胞累积至少10重量％的S- 腺苷甲硫氨酸，从培养基中分离酵母细胞，然后从酵母细胞中获得稳定形式的S-腺苷甲硫氨酸。

公开(公告)号：US4269942A

公开(公告)日：1981-05-26

申请号：US132177

申请日：1980-03-20

申请人： Hideaki Yamada ,  Sakayu Shimizu ,  Yoshiki Tani

发明人： Hideaki Yamada ,  Sakayu Shimizu ,  Yoshiki Tani

IPC分类号： C12N9/00 ,  C12Q1/25 ,  C12N9/10

CPC分类号： C12N9/93 ,  C12Q1/25 ,  Y10S435/875 ,  Y10S435/911

摘要： Acyl-CoA synthetase, having a high activity to C.sub.16 -C.sub.18 long chain fatty acids, is obtained by cultivating Pseudomonas aeruginosa IFO 3919. Pseudomonas aeruginosa IFO 3919 can produce acyl-CoA synthetase LCF-18 in large quantities, and the acyl-CoA synthetase LCF-18 produced by Pseudomonas aeruginosa IFO 3919 is very stable in storage.

摘要翻译： 通过培养铜绿假单胞菌IFO 3919获得对C16-C18长链脂肪酸具有高活性的酰基辅酶A合成酶。铜绿假单胞菌IFO 3919可以大量产生酰基辅酶A合成酶LCF-18，酰基辅酶A合成酶 由绿脓假单胞菌IFO 3919生产的LCF-18在储存中非常稳定。

公开(公告)号：US09410241B2

公开(公告)日：2016-08-09

申请号：US12439887

申请日：2007-08-31

申请人： Yoshiaki Mokuno ,  Akiyoshi Chayahara ,  Hideaki Yamada

发明人： Yoshiaki Mokuno ,  Akiyoshi Chayahara ,  Hideaki Yamada

IPC分类号： C23C16/01 ,  C23C16/27 ,  C23C16/56 ,  C25F3/00 ,  C30B29/04 ,  C30B31/22 ,  C30B33/06

CPC分类号： C23C16/01 ,  C23C16/27 ,  C23C16/56 ,  C25F3/00 ,  C30B29/04 ,  C30B31/22 ,  C30B33/06

摘要： The present invention provides a method for separating a surface layer of a diamond, which comprises implanting ions into a diamond to form a non-diamond layer near a surface of the diamond; and etching the non-diamond layer in the diamond by applying an alternating-current voltage across electrodes in an electrolytic solution; and a method for separating a grown layer of a diamond, which further comprises the step of growing a diamond by a vapor-phase synthesis method, after forming a non-diamond layer according to the above-described method. The invention is applicable to various single-crystal and polycrystal diamonds. More specifically, even with a large single-crystal diamond, a portion of the single-crystal diamond can be efficiently separated in a reusable form in a relatively short period of time.

摘要翻译： 本发明提供一种用于分离金刚石的表面层的方法，其包括将离子注入金刚石以在金刚石的表面附近形成非金刚石层; 以及通过在电解液中跨越电极施加交流电压来蚀刻金刚石中的非金刚石层; 以及分离金刚石生长层的方法，其还包括根据上述方法在形成非金刚石层之后通过气相合成法生长金刚石的步骤。 本发明适用于各种单晶和多晶钻石。 更具体地，即使使用大的单晶金刚石，一部分单晶金刚石可以在相对较短的时间内以可重复使用的形式被有效地分离。