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    Apparatus for automated assay of DNA probe and method for assaying nucleic acid in sample
    2.
    发明授权
    Apparatus for automated assay of DNA probe and method for assaying nucleic acid in sample 失效
    用于DNA探针自动化测定的装置和样品中核酸测定方法

    公开(公告)号：US5538849A

    公开(公告)日：1996-07-23

    申请号：US174308

    申请日：1993-12-29

    申请人： Hiroaki Uematsu ,  Makoto Tsuruoka ,  Takashi Nakajima ,  Ken-ichi Chikanari ,  Misao Yoshimoto ,  Hiromichi Kobata

    发明人： Hiroaki Uematsu ,  Makoto Tsuruoka ,  Takashi Nakajima ,  Ken-ichi Chikanari ,  Misao Yoshimoto ,  Hiromichi Kobata

    IPC分类号： C12Q1/68 ,  G01N35/02 ,  C12N15/00 ,  G01N21/00 ,  G01N35/00

    CPC分类号： G01N35/026 ,  C12Q1/6816 ,  Y10T436/11 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/143333

    摘要： An apparatus is presented for an automated assay of a DNA probe, which is performed by hybridizing a nucleic acid in a sample with a DNA probe marked with an enzyme and optically measuring a change that occurs in the substrate by catalysis of the enzyme which is bound with the DNA probe. The DNA probe hybridizes with the nucleic acid in the sample to assay the nucleic acid in the sample. Comprising (A) a sample-reagent unit, (B) a sample-reagent dispense unit, (C) a reaction vessel transport unit, (D) a hybridization unit, (E) a B/F separation unit, and (F) a light measurement unit; also presented is a method for assaying a nucleic acid in a sample by the apparatus of the present invention. According to the measurement apparatus of the present invention, a continuous and highly precise measurement is attainable. In particular, the light-shielding structure of the light measurement unit exhibits high levels of light-shielding effect and contributes to efficient light measurement and prevention of degradation of light receiving elements.

    摘要翻译： 提供了一种用于DNA探针的自动测定的装置，其通过将样品中的核酸与标记有酶的DNA探针杂交并通过催化结合的酶光学测量在底物中发生的变化来进行 与DNA探针。 DNA探针与样品中的核酸杂交以测定样品中的核酸。 包括（A）样品试剂单元，（B）样品试剂分配单元，（C）反应容器输送单元，（D）杂交单元，（E）B / F分离单元和（F） 光测量单元; 还提出了通过本发明的装置测定样品中的核酸的方法。 根据本发明的测量装置，可以获得连续高精度的测量。 特别地，光测量单元的遮光结构具有高水平的遮光效果，并且有助于有效的光测量和防止光接收元件的劣化。

    Flow through system liquid membrane type electrode
    3.
    发明授权
    Flow through system liquid membrane type electrode 失效
    流经系统液膜式电极

    公开(公告)号：US4551222A

    公开(公告)日：1985-11-05

    申请号：US622262

    申请日：1984-06-19

    申请人： Hiroaki Uematsu ,  Junji Aoki

    发明人： Hiroaki Uematsu ,  Junji Aoki

    IPC分类号： G01N27/28 ,  G01N27/333 ,  G01N27/46

    CPC分类号： G01N27/3335 ,  G01N27/28

    摘要： A flow through system liquid membrane type electrode used for measuring a specific ion concentration in a flowing liquid sample. A cylindrical membrane holding member which is flat at one side surface, holds a sheet-like liquid membrane, the liquid membrane facing a liquid sample passage formed in a synthetic resin block, thereby to form a strong junction between the liquid membrane and the sample passage.

    摘要翻译： 用于测量流动液体样品中特定离子浓度的流通系统液膜型电极。 在一个侧面平坦的圆筒状的膜保持部件保持片状的液膜，液膜面对形成在合成树脂块中的液体样品通道，从而在液体膜和样品通道之间形成强连接 。

    Method of measuring electrolyte in blood and urine by electrode method
    4.
    发明授权
    Method of measuring electrolyte in blood and urine by electrode method 失效
    通过电极法测定血液和尿液中的电解质的方法

    公开(公告)号：US4877492A

    公开(公告)日：1989-10-31

    申请号：US204994

    申请日：1988-06-10

    申请人： Hiroaki Uematsu ,  Shinji Imanishi

    发明人： Hiroaki Uematsu ,  Shinji Imanishi

    IPC分类号： G01N33/483 ,  G01N27/26 ,  G01N27/27 ,  G01N27/416 ,  G01N33/487 ,  G01N33/49

    CPC分类号： G01N33/49 ,  G01N33/493 ,  Y10T436/19 ,  Y10T436/193333 ,  Y10T436/25625

    摘要： Method of measuring electrolyte concentration in a urine sample, by preparing a calibrating solution containing ions of the electrolytes to be measured, preparing a diluent containing the same ions, diluting the urine sample with the diluent, measuring the electrolyte in the diluted urine with an electrode using the calibrating solution, and calculating the concentration of electrolyte based on the measured value. Additionally, the concentration of the same electrolytes in a blood sample can be measured without dilution, using the same calibrating solution.

    摘要翻译： 通过制备含有待测电解质的离子的校准溶液，制备含有相同离子的稀释剂，用稀释剂稀释尿样，用电极测量稀释尿液中的电解质，测定尿样中的电解质浓度的方法 使用校准溶液，并根据测量值计算电解液的浓度。 此外，使用相同的校准溶液，可以在不稀释的情况下测量血液样品中相同电解质的浓度。

    Flow through type ion electrode
    5.
    发明授权
    Flow through type ion electrode 失效
    流通型离子电极

    公开(公告)号：US4935117A

    公开(公告)日：1990-06-19

    申请号：US370449

    申请日：1989-06-23

    申请人： Hiroaki Uematsu ,  Kunio Terada

    发明人： Hiroaki Uematsu ,  Kunio Terada

    IPC分类号： G01N27/28

    CPC分类号： G01N27/28

    摘要： A flow through type ion electrode apparatus including a number of interconnectable block structures. Each block structure includes recesses in respective faces thereof, which lead to a through hole extending between the faces. A membrane electrode is located within each block structure so as to communicate with the through hole. A spacer having a through hole therein is inserted in a recess of each of a pair of adjacent block structures to interconnect the adjacent block structures.

    摘要翻译： 一种流通式离子电极装置，包括多个可互连块结构。 每个块结构包括在其各自的表面中的凹槽，其导致在两个面之间延伸的通孔。 膜电极位于每个块结构内，以便与通孔连通。 在其中具有通孔的间隔件插入在一对相邻块结构中的每一个的凹部中以互连相邻的块结构。

    Method for producing a response membrane for use in a chloride ion selective electrode
    6.
    发明授权
    Method for producing a response membrane for use in a chloride ion selective electrode 失效
    用于生产用于氯离子选择性电极的响应膜的方法

    公开(公告)号：US4629744A

    公开(公告)日：1986-12-16

    申请号：US714082

    申请日：1985-03-20

    申请人： Hiroaki Uematsu ,  Takeshi Kohno ,  Takeshi Miyazaki

    发明人： Hiroaki Uematsu ,  Takeshi Kohno ,  Takeshi Miyazaki

    IPC分类号： G01N27/333 ,  C08J9/28

    CPC分类号： G01N27/3335

    摘要： A composition for use in a chloride ion electrode is provided herein. This composition is made up of a mixture of an epoxy resin, a vinyl chloride resin, one or more solvents, a quaternary ammonium salt and hardening agents for setting said epoxy resin; wherein said epoxy resin and the vinyl chloride resin is used at a ratio of 8:1 to 1:2 by weight. The present invention also relates to a response membrane produced from said composition and a method for producing the response membrane by setting or hardening the aforementioned composition. When the response membrane is used in the chloride ion selective electrode, it is capable of measuring a sample containing blood or protein and by use of the response membrane, it is possible to selectively determine chloride ions in a sample with a high degree of accuracy.

    摘要翻译： 本文提供了一种用于氯离子电极的组合物。 该组合物由环氧树脂，氯乙烯树脂，一种或多种溶剂，季铵盐和用于固化所述环氧树脂的硬化剂的混合物组成; 其中所述环氧树脂和氯乙烯树脂以8:1至1:2的比例使用。 本发明还涉及由所述组合物制备的反应膜和通过凝固或硬化上述组合物来制备响应膜的方法。 当在氯离子选择电极中使用响应膜时，其能够测量含有血液或蛋白质的样品，并且通过使用响应膜，可以以高精度选择性地确定样品中的氯离子。

    Standard solution for simultaneously calibrating a plurality of ion electrodes
    9.
    发明授权
    Standard solution for simultaneously calibrating a plurality of ion electrodes 失效
    用于同时校准多个离子电极的标准溶液

    公开(公告)号：US4626512A

    公开(公告)日：1986-12-02

    申请号：US737036

    申请日：1985-05-22

    申请人： Narihiro Oku ,  Seiji Usui ,  Hiroaki Uematsu ,  Takeshi Kohno

    发明人： Narihiro Oku ,  Seiji Usui ,  Hiroaki Uematsu ,  Takeshi Kohno

    IPC分类号： G01N27/26 ,  G01N27/416 ,  G01N33/84 ,  G01N31/00

    CPC分类号： G01N27/4165 ,  G01N33/84 ,  Y10T436/10 ,  Y10T436/108331

    摘要： A standard solution for simultaneously calibrating a plurality of ion electrodes for determining a plurality of ion electrodes for determining a plurality of ions including at least potassium ions and calcium ions in blood, the improvement wherein the concentration of the standard solution for a potassium ion electrode and a calcium ion electrode is adjusted in accordance with the following equations: ##EQU1## As for potassium ion,C=C.sub.o .multidot.10.sup.-.DELTA.E/60As for calcium ion,C=C.sub.o .multidot.10.sup.-.DELTA.E/30As for potassium ion,A=-0.130 to -0.065As for calcium ion,A=-0.240 to -0.172whereinE: is the potential difference when an ionic strength of 150 mM is used as the standard;.mu.: is the ionic strength;C.sub.o : is the calibrated concentration;C: is the concentration of reagent added; andA: is the correction factor.

    摘要翻译： 一种用于同时校准多个离子电极以用于确定多个离子电极以用于确定血液中至少包含钾离子和钙离子的多个离子的标准溶液，其中所述改进是将钾离子电极的标准溶液和 钙离子电极根据以下等式进行调整：对于钾离子，C = Co×10 -3 DELTA E / 60对于钙离子，C = Co×10 -3 DELTA E / 30对于钾离子，A = - 0.130〜-0.065对于钙离子，A = -0.240〜-0.172其中E：当以150mM的离子强度作为标准时的电位差; mu：离子强度; Co：是校准浓度; C：加入试剂的浓度; A：是校正因子。

    Flow system glass electrode
    10.
    发明授权
    Flow system glass electrode 失效
    流量系玻璃电极

    公开(公告)号：US4519890A

    公开(公告)日：1985-05-28

    申请号：US614225

    申请日：1984-05-24

    申请人： Hiroaki Uematsu ,  Junji Aoki ,  Narihiro Oku

    发明人： Hiroaki Uematsu ,  Junji Aoki ,  Narihiro Oku

    IPC分类号： G01N27/28 ,  G01N27/36

    CPC分类号： G01N27/28 ,  G01N27/36

    摘要： A flow system glass electrode in use for pH measurement, which houses the glass electrode body in a resin block to improve an impact resistance, the glass electrode body being sealed by a packing to be small-sized and improve the degree of freedom in the direction of the use, sample flow tubes each being equal in an inner diameter to a response film and separate from the glass electrode body in order to eliminate stagnation of a sample liquid, enable the measurement of a minute sample, and facilitate manufacture and assembly of the electrode, the sample flow tubes being connected with a liquid inlet and a liquid outlet at the glass electrode body through couplings respectively.

    摘要翻译： 一种用于pH测量的流动系统玻璃电极，其将玻璃电极体容纳在树脂块中以提高耐冲击性，玻璃电极体被填料密封成小尺寸并提高了方向的自由度 使用时，样品流管​​各自在内径相等于响应膜并与玻璃电极体分离，以消除样品液体的滞留，使得能够测量微小的样品，并且有助于制造和组装 电极，样品流管​​分别通过耦合与玻璃电极体的液体入口和液体出口连接。