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110 条记录 (耗时 0.038 秒)

    Ligation-Based Rna Amplification
    1.
    发明申请
    Ligation-Based Rna Amplification 审中-公开
    基于连接的Rna扩增

    公开(公告)号：US20080003602A1

    公开(公告)日：2008-01-03

    申请号：US11722089

    申请日：2005-12-22

    申请人： John Nelson ,  R. Duthie ,  Rohini Dhulipala ,  Gregory Grossmann ,  Anuradha Sekher

    发明人： John Nelson ,  R. Duthie ,  Rohini Dhulipala ,  Gregory Grossmann ,  Anuradha Sekher

    IPC分类号： C12Q1/68 ,  C12N9/00 ,  C12N9/12 ,  C12P19/34

    CPC分类号： C12N15/1096 ,  C12Q1/6865 ,  C12Q2525/143 ,  C12Q2521/501

    摘要： Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.

    摘要翻译： 描述了扩增，纯化和检测核酸序列特别是RNA的方法。 该方法的一个方面涉及将核酸结构杂交并随后连接到期望操作的核酸序列。 所述方法要求核酸结构包含双链区域和单链区域。 单链区域与感兴趣的RNA序列互补。 双链区域还可以包含随后在该方法中使用的另外的官能团。

    Nucleic acid amplification with terminal-phosphate labeled nucleotides
    2.
    发明授权
    Nucleic acid amplification with terminal-phosphate labeled nucleotides 有权
    用磷酸末端标记的核苷酸扩增核酸

    公开(公告)号：US07125671B2

    公开(公告)日：2006-10-24

    申请号：US10651362

    申请日：2003-08-29

    申请人： Anup Sood ,  Shiv Kumar ,  John Nelson ,  Carl Fuller ,  Anuradha Sekher

    发明人： Anup Sood ,  Shiv Kumar ,  John Nelson ,  Carl Fuller ,  Anuradha Sekher

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q2525/101 ,  C12Q2521/525 ,  C12Q2521/101 ,  C12Q2565/301 ,  C12Q2561/113

    摘要： The present invention relates generally to the use of terminal-phosphate-labeled nucleotides having three or more phosphates as substrates for nucleic acid polymerases and their use in DNA amplification. The labels employed are chemiluminescent, fluorescent, electrochemical and chromogenic moieties as well as mass tags and include those that are directly detectable, detectable after enzyme activation or feed into other processes to generate a different signal. The signal generated from the attached dyes may also be used to quantify the amount of amplification. Further provided are stabilizers that enhance the stability of terminal-phosphate labeled nucleoside polyphosphates in aqueous solutions and are useful for reducing non-enzymatic hydrolysis of these nucleotides, hence decrease background.

    摘要翻译： 本发明一般涉及具有三种或更多种磷酸盐的末端磷酸酯标记的核苷酸作为核酸聚合酶的底物及其在DNA扩增中的应用。 使用的标记是化学发光，荧光，电化学和显色部分以及质量标签，并且包括直接可检测的标记，可在酶活化后进行或进入其它过程以产生不同信号。 从连接的染料产生的信号也可用于量化扩增量。 还提供了增强末端磷酸酯标记的核苷多磷酸酯在水溶液中的稳定性的稳定剂，并且可用于减少这些核苷酸的非酶水解，从而降低背景。

    Soldering Jig Assembly
    3.
    发明申请
    Soldering Jig Assembly 审中-公开

    公开(公告)号：US20180229323A1

    公开(公告)日：2018-08-16

    申请号：US15432237

    申请日：2017-02-14

    申请人： John Nelson

    发明人： John Nelson

    IPC分类号： B23K3/08 ,  B23K101/38

    摘要： A soldering jig assembly for soldering conductors together includes a plate that may be positioned on a support surface. A pair of gripping units is provided. Each of the gripping units is coupled to the plate and each of the gripping units is selectively manipulated. Each of the gripping units engages an associated one of a pair of conductors.

    Allele specific primer extension
    8.
    发明授权
    Allele specific primer extension 有权
    等位基因特异性引物延伸

    公开(公告)号：US07560254B2

    公开(公告)日：2009-07-14

    申请号：US10651558

    申请日：2003-08-29

    申请人： Anup Sood ,  Shiv Kumar ,  Carl Fuller ,  John Nelson ,  John Macklin

    发明人： Anup Sood ,  Shiv Kumar ,  Carl Fuller ,  John Nelson ,  John Macklin

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

    CPC分类号： C12Q1/6853 ,  C12Q1/6858 ,  C12Q2525/125 ,  C12Q2521/525 ,  C12Q2521/319

    摘要： A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, an allele specific primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and optionally an enzyme having 3′→5′ exonuclease activity when the primer is non-hydrolyzable, which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on such detection.

    摘要翻译： 提供了表征核酸样品的方法，其包括以下步骤：（a）进行DNA聚合酶反应，其包括模板，等位基因特异性引物，至少一个末端磷酸酯标记的核苷酸，DNA聚合酶和 任选地当引物不可水解时具有3'→5'核酸外切酶活性的酶，该反应导致标记的多磷酸盐的产生; （b）允许标记的多磷酸酯与磷酸酶反应以产生可检测的物质; （c）检测可检测物种; 和（d）基于这种检测来表征核酸样品。

    Amplification of self-ligated, circularized cDNA for expression profiling
    9.
    发明授权
    Amplification of self-ligated, circularized cDNA for expression profiling 有权
    用于表达谱的自连接的环化cDNA的扩增

    公开(公告)号：US07309571B2

    公开(公告)日：2007-12-18

    申请号：US10770657

    申请日：2004-02-03

    申请人： John Nelson

    发明人： John Nelson

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6862 ,  C12Q1/689 ,  C12Q2525/307 ,  C12Q2521/501

    摘要： This invention presents a new cDNA amplification method. RNA is first converted to cDNA. The synthesis of cDNA can include a promoter tagged oligonucleotide, and then this cDNA is ligated to form circles (or possibly concatemers). This is then amplified using a Phi29 DNA polymerase based rolling circle and strand displacement amplification. The invention allows for RNA promoter sequences to be attached to the cDNA to facilitate additional amplification through the generation of RNA from the amplified cDNA. The resulting product can then be used to make materials for gene expression studies or other RNA analysis procedures.

    摘要翻译： 本发明提出了一种新的cDNA扩增方法。 RNA首先转化为cDNA。 cDNA的合成可以包括启动子标记的寡核苷酸，然后将该cDNA连接形成环（或可能是连接体）。 然后使用基于Phi29 DNA聚合酶的滚动圆和链置换扩增来扩增。 本发明允许将RNA启动子序列连接到cDNA，以便通过从扩增的cDNA产生RNA来进一步扩增。 所得产物可用于制备用于基因表达研究或其他RNA分析程序的材料。

    INSECT CONTROL COMPOSITIONS
    10.
    发明申请
    INSECT CONTROL COMPOSITIONS 审中-公开
    防护组合物

    公开(公告)号：US20070264294A1

    公开(公告)日：2007-11-15

    申请号：US11382574

    申请日：2006-05-10

    申请人： Edward Lance-Gomez ,  Anne Maghasi ,  John Nelson

    发明人： Edward Lance-Gomez ,  Anne Maghasi ,  John Nelson

    IPC分类号： A01N25/00

    CPC分类号： A01N25/06 ,  A61K8/046 ,  A61K8/34 ,  A61K8/42 ,  A61K8/4926 ,  A61K8/732 ,  A61Q17/02 ,  A01N25/02 ,  A01N25/04 ,  A01N25/08 ,  A01N25/10 ,  A01N31/06 ,  A01N37/18 ,  A01N37/46 ,  A01N47/16

    摘要： Disclosed herein are sprayable insect repellent compositions that provide improved skin feel through the incorporation of a powder ingredient. The powder is essentially permanently suspended in the composition by a thickener (e.g. carbomer thickener) so that no shaking to resuspend settled out powder is necessary immediately before the composition is applied to the skin. This is achieved in an aerosol or spritz spray formulation, while avoiding clogging problems. One composition contains ethanol, DEET or picaridin, carbomer thickener, a neutralizer, corn starch powder, water, and gas propellant.

    摘要翻译： 本文公开了可喷雾的驱虫剂组合物，其通过掺入粉末成分来提供改善的皮肤感觉。 该粉末通过增稠剂（例如卡波姆增稠剂）基本上永久地悬浮在组合物中，使得在将组合物施用于皮肤之前不需要立即振荡以重悬沉淀出的粉末。 这可以在气雾剂或喷雾剂喷雾配方中实现，同时避免堵塞问题。 一种组合物含有乙醇，DEET或氨基吡啶，卡波姆增稠剂，中和剂，玉米淀粉粉末，水和气体推进剂。