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51 条记录 (耗时 0.048 秒)

    Direct, externally imposed control of polypeptides
    2.
    发明授权
    Direct, externally imposed control of polypeptides 失效
    直接的外部控制多肽

    公开(公告)号：US06953656B2

    公开(公告)日：2005-10-11

    申请号：US09905831

    申请日：2001-07-13

    申请人： Joseph M. Jacobson ,  John J. Schwartz ,  Kimberly Hamad ,  Shuguang Zhang

    发明人： Joseph M. Jacobson ,  John J. Schwartz ,  Kimberly Hamad ,  Shuguang Zhang

    IPC分类号： C07K14/47 ,  C12N13/00 ,  C12Q1/00 ,  C12Q1/18

    CPC分类号： C12N13/00 ,  C07K14/47 ,  C12N2310/351 ,  C12N2310/53 ,  Y10S204/904

    摘要： Methods and compositions for rendering proteins directly responsive to an external signal utilizing modulators that themselves respond to the external signal and are associated with the proteins. In response to the external signal, the modulator alters physical properties of the specific protein molecule(s) with which it is associated, thereby altering the structural and functional properties thereof. The modulator may, for example, transfer applied energy to a protein, or to a portion of the protein, thereby changing the protein structure and function.

    摘要翻译： 使用本身对外部信号响应并与蛋白质相关的调节剂使蛋白质直接响应于外部信号的方法和组合物。 响应于外部信号，调节剂改变与其相关联的特定蛋白质分子的物理性质，从而改变其结构和功能性质。 调节剂可以例如将施加的能量转移到蛋白质或蛋白质的一部分，从而改变蛋白质结构和功能。

    Modular peptide mediated intracellular delivery system and uses therefore
    3.
    发明授权
    Modular peptide mediated intracellular delivery system and uses therefore 有权
    模块化肽介导的细胞内传递系统和用途

    公开(公告)号：US06844324B1

    公开(公告)日：2005-01-18

    申请号：US09438905

    申请日：1999-11-12

    申请人： Shuguang Zhang ,  John J. Schwartz

    发明人： Shuguang Zhang ,  John J. Schwartz

    IPC分类号： A61K48/00 ,  C07H21/04 ,  C12N15/87 ,  C12Q1/68

    CPC分类号： C12N15/87 ,  A61K48/00 ,  C07H21/04

    摘要： A versatile modular peptide mediated intracellular delivery system is disclosed which may be particularly adapted to facilitate the delivery of therapeutic compounds which are large in size or complex in nature. The invention relates both to a modular peptide mediated intracellular delivery system and a method of delivering a compound into a cell using the system.

    摘要翻译： 公开了通用的模块化肽介导的细胞内递送系统，其可以特别适于促进大小或复合物性质大的治疗化合物的递送。 本发明涉及模块化肽介导的细胞内递送系统和使用该系统将化合物递送到细胞中的方法。

    SELF-ASSEMBLING PEPTIDE INCORPORATING MODIFICATIONS AND METHODS OF USE THEREOF
    5.
    发明申请
    SELF-ASSEMBLING PEPTIDE INCORPORATING MODIFICATIONS AND METHODS OF USE THEREOF 有权
    自组装肽的修改及其使用方法

    公开(公告)号：US20100311640A1

    公开(公告)日：2010-12-09

    申请号：US12724153

    申请日：2010-03-15

    申请人： Elsa Genove ,  Shuguang Zhang ,  Carlos Semino

    发明人： Elsa Genove ,  Shuguang Zhang ,  Carlos Semino

    IPC分类号： A61K38/02 ,  C07K2/00 ,  C07K14/00 ,  C12N5/00 ,  A61P35/00 ,  A61P13/12 ,  C07K7/08 ,  C07K7/06 ,  C07K5/10 ,  C07K5/08

    CPC分类号： C07K7/08 ,  A61K38/00 ,  C07K7/06 ,  C07K14/78 ,  C07K2319/735

    摘要： The invention provides a self-assembling peptide comprising (a) a first amino acid domain that mediates self-assembly, wherein the domain comprises alternating hydrophobic and hydrophilic amino acids that are complementary and structurally compatible and self-assemble into a macroscopic structure when present in unmodified form; and (b) a second amino acid domain that does not self-assemble in isolated form. In certain embodiments of the invention the second amino acid domain comprises a biologically active peptide motif, e.g., a peptide motif found in a naturally occurring protein, or a target site for an interaction with a biomolecule. In certain embodiments of the invention the naturally occurring protein is a component of the extracellular matrix, e.g., a component of the basement membrane. The invention further provides scaffolds comprising the self-assembling peptides and methods of using the scaffolds including for cell culture, tissue engineering, and tissue repair.

    摘要翻译： 本发明提供一种自组装肽，其包含（a）介导自组装的第一氨基酸结构域，其中所述结构域包含互补和结构相容的交替的疏水性和亲水性氨基酸，并且当存在于 未经修改的形式; 和（b）不以分离形式自组装的第二个氨基酸结构域。 在本发明的某些实施方案中，第二氨基酸结构域包含生物活性肽基序，例如在天然存在的蛋白质中发现的肽基序，或与生物分子相互作用的靶位点。 在本发明的某些实施方案中，天然存在的蛋白质是细胞外基质的组分，例如基底膜的组分。 本发明还提供了包含自组装肽的支架和使用支架的方法，包括用于细胞培养，组织工程和组织修复。

    Stable macroscopic membranes formed by self-assembly of amphiphilic peptides and uses therefor
    7.
    发明申请
    Stable macroscopic membranes formed by self-assembly of amphiphilic peptides and uses therefor 审中-公开
    通过两亲肽的自组装形成稳定的宏观膜并用于其

    公开(公告)号：US20070190603A1

    公开(公告)日：2007-08-16

    申请号：US11512753

    申请日：2006-08-29

    申请人： Todd Holmes ,  Shuguang Zhang ,  Alexander Rich ,  C. DiPersio ,  Curtis Lockshin

    发明人： Todd Holmes ,  Shuguang Zhang ,  Alexander Rich ,  C. DiPersio ,  Curtis Lockshin

    IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N5/08 ,  C07K14/47

    CPC分类号： C07K7/08 ,  A61K9/009 ,  C07K5/0815 ,  C07K7/06 ,  C07K14/001 ,  C07K14/395 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/50

    摘要： Described herein is the self-assembly of amphiphilic peptides, i.e., peptides with alternating hydrophobic and hydrophilic residues, into macroscopic membranes. The membrane-forming peptides are greater than 12 amino acids in length, and preferably at least 16 amino acids, are complementary and are structurally compatible. Specifically, two peptides, (AEAEAKAK)2 (ARARADAD)2, were shown to self-assemble into macroscopic membranes. Conditions under which the peptides self-assemble into macroscopic membranes and methods for producing the membranes are also described. The macroscopic membranes have several interesting properties: they are stable in aqueous solution, serum, and ethanol, are highly resistant to heat, alkaline and acidic pH, chemical denaturants, and proteolytic digestion, and are non-cytotoxic. The membranes are potentially useful in biomaterial applications such as slow-diffusion drug delivery systems, artificial skin, and separation matrices, and as experimental models for Alzheimer's disease and scrapie infection. The sequence of the peptide, EAK16, was derived from a putative Z-DNA binding protein from yeast, called zuotin. The cloning and characterization of the ZUO1 gene are also described.

    摘要翻译： 这里描述的是将两亲肽，即具有交替疏水和亲水残基的肽自组装成宏观膜。 成膜肽长度大于12个氨基酸，优选至少16个氨基酸是互补的并且在结构上相容。 具体来说，显示两种肽（AEAEAKAK）2（ARARADAD）2 N自组装成宏观膜。 还描述了肽自组装成宏观膜的条件和制备膜的方法。 肉眼膜具有几个有趣的性质：它们在水溶液，血清和乙醇中稳定，对热，碱性和酸性pH，化学变性剂和蛋白水解消化具有高度抗性，并且是非细胞毒性的。 该膜可用于生物材料应用，如慢扩散药物递送系统，人造皮肤和分离基质，以及阿尔茨海默病和瘙痒病感染的实验模型。 肽EAK16的序列衍生自称为佐酮的来自酵母的推定的Z-DNA结合蛋白。 还描述了ZUO1基因的克隆和表征。

    Stable macroscopic membranes formed by self-assembly of amphiphilic peptides and uses therefor
    9.
    发明授权
    Stable macroscopic membranes formed by self-assembly of amphiphilic peptides and uses therefor 失效
    通过两亲肽的自组装形成稳定的宏观膜并用于其

    公开(公告)号：US06548630B1

    公开(公告)日：2003-04-15

    申请号：US08898300

    申请日：1997-07-22

    申请人： Shuguang Zhang ,  Curtis Lockshin ,  Alexander Rich ,  Todd Holmes

    发明人： Shuguang Zhang ,  Curtis Lockshin ,  Alexander Rich ,  Todd Holmes

    IPC分类号： C07K700

    CPC分类号： C07K7/08 ,  A61K9/009 ,  C07K14/001 ,  C07K14/395 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/50

    摘要： Described herein is the self-assembly of amphiphilic peptides, i.e., peptides with alternating hydrophobic and hydrophilic residues, into macroscopic membranes. The membrane-forming peptides are greater than 12 amino acids in length, and preferably at least 16 amino acids, are complementary and are structurally compatible. Specifically, two peptides, (AEAEAKAK)2 (ARARADAD)2, were shown to self-assemble into macroscopic membranes. Conditions under which the peptides self-assemble into macroscopic membranes and methods for producing the membranes are also described. The macroscopic membranes have several interesting properties: they are stable in aqueous solution, serum, and ethanol, are highly resistant to heat, alkaline and acidic pH, chemical denaturants, and proteolytic digestion, and are non-cytotoxic. The membranes are potentially useful in biomaterial applications such as slow-diffusion drug delivery systems, artificial skin, and separation matrices, and as experimental models for Alzheimer's disease and scrapie infection. The sequence of the peptide, EAK16, was derived from a putative Z-DNA binding protein from yeast, called zuotin. The cloning and characterization of the ZUO1 gene are also described.

    摘要翻译： 这里描述的是将两亲肽，即具有交替疏水和亲水残基的肽自组装成宏观膜。 成膜肽长度大于12个氨基酸，优选至少16个氨基酸是互补的并且在结构上相容。 具体来说，显示两种肽（AEAEAKAK）2（ARARADAD）2自组装成宏观膜。 还描述了肽自组装成宏观膜的条件和制备膜的方法。 肉眼膜具有几个有趣的性质：它们在水溶液，血清和乙醇中稳定，对热，碱性和酸性pH，化学变性剂和蛋白水解消化具有高度抗性，并且是非细胞毒性的。 该膜可用于生物材料应用，如慢扩散药物递送系统，人造皮肤和分离基质，以及阿尔茨海默病和瘙痒病感染的实验模型。 肽EAK16的序列衍生自称为佐酮的来自酵母的推定的Z-DNA结合蛋白。 还描述了ZUO1基因的克隆和表征。