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    Caged nucleotides
    1.
    发明授权
    Caged nucleotides 失效
    笼状核苷酸

    公开(公告)号：US5872243A

    公开(公告)日：1999-02-16

    申请号：US497183

    申请日：1995-06-30

    申请人： Kyle R. Gee ,  Hon Cheung Lee ,  Robert Aarhus ,  Richard P. Haugland ,  Timothy F. Walseth ,  Richard M. Graeff

    发明人： Kyle R. Gee ,  Hon Cheung Lee ,  Robert Aarhus ,  Richard P. Haugland ,  Timothy F. Walseth ,  Richard M. Graeff

    IPC分类号： C07H19/20 ,  C07H21/02 ,  C07H1/00 ,  C07H21/00

    CPC分类号： C07H19/20 ,  C07H21/02

    摘要： The present invention describes a family of photolabile caged nucleotides, including cyclic nucleotides. The compounds of the present invention are caged analogs and derivatives of NAD.sup.+, NADH, NADP, NADPH, NAADP and cADPR. The photolysis of the present compounds allows the release of the free nucleotide in vivo or in vitro with precise spatial and temporal control. The compounds are useful for the photolytic generation of free nucleotides in aqueous samples, for example, in the study of calcium mobilization in cells and cell homogenates.

    摘要翻译： 本发明描述了一种包含环状核苷酸的光不稳定笼状核苷酸家族。 本发明的化合物是NAD +，NADH，NADP，NADPH，NAADP和cADPR的笼养类似物和衍生物。 本发明化合物的光解使得体内或体外释放游离核苷酸具有精确的空间和时间控制。 这些化合物可用于水溶液样品中游离核苷酸的光解产生，例如在细胞和细胞匀浆中钙迁移的研究中。

    Crown ether derivatives
    4.
    发明授权
    Crown ether derivatives 失效
    冠醚衍生物

    公开(公告)号：US06962992B2

    公开(公告)日：2005-11-08

    申请号：US10026302

    申请日：2001-12-19

    申请人： Vladimir V. Martin ,  Kyle R. Gee ,  Richard P. Haugland ,  Zhenjun Diwu

    发明人： Vladimir V. Martin ,  Kyle R. Gee ,  Richard P. Haugland ,  Zhenjun Diwu

    IPC分类号： C07D273/00 ,  C07H21/00 ,  C09B57/10 ,  C09B69/00 ,  G01N33/58 ,  G01N33/84 ,  C07D413/04 ,  C07D273/08 ,  C07D317/28

    CPC分类号： A61K49/0052 ,  A61K49/0021 ,  A61K49/0041 ,  C07D273/00 ,  C07H21/00 ,  C09B11/24 ,  C09B23/04 ,  C09B57/00 ,  C09B57/10 ,  C09B69/00 ,  G01N33/582 ,  G01N33/84

    摘要： The invention describes crown ether chelators, including crown ethers having the formula and aza-substituted and thia-substituted analogs thereof. These crown ethers are substituted by a dye moiety, a chemically reactive group, a conjugated substance, or a combination thereof. Chelators that are substituted by fluorescent dyes are particularly useful as indicators for metal cations, particularly Na+ and K+ ions, and particularly where binding of the target ion results in a change in the fluorescence properties of the indicator that can be correlated with the ion concentration. Methods are provided for utilizing reactive groups on the chelators for conjugation to dyes, lipids and polymers and methods for enhancing entry of the indicators into living cells.

    摘要翻译： 本发明描述了冠醚螯合剂，包括具有下式的冠醚和其氮杂取代和硫取代的类似物。 这些冠醚被染料部分，化学反应性基团，共轭物质或其组合取代。 被荧光染料取代的螯合剂特别可用作金属阳离子，特别是Na +和K + +离子的指示剂，特别是当目标离子的结合导致变化时 在可以与离子浓度相关的指示剂的荧光特性中。 提供了利用螯合剂上的反应性基团与染料偶联的方法，脂质和聚合物以及用于增强指示剂进入活细胞的方法。

    Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
    5.
    发明授权
    Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings 有权
    含稠环的1,2-二氢-7-羟基喹啉的衍生物

    公开(公告)号：US06716979B2

    公开(公告)日：2004-04-06

    申请号：US09922333

    申请日：2001-08-04

    申请人： Zhenjun Diwu ,  Jiaxing Liu ,  Richard P. Haugland ,  Kyle R. Gee

    发明人： Zhenjun Diwu ,  Jiaxing Liu ,  Richard P. Haugland ,  Kyle R. Gee

    IPC分类号： C07D49122

    CPC分类号： C07D471/04 ,  C07D491/04 ,  C07D495/04 ,  C07D495/14 ,  G01N33/582 ,  Y10S436/80

    摘要： The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.

    摘要翻译： 本发明描述了新颖的染料，包括掺入另外的稠合芳环的香豆素，罗丹明和罗丹醇。 本发明的染料比不具有稠合芳环的结构相似的染料长的波长吸收。 本发明的许多染料是有用的荧光染料。 本发明包括化学活性染料，染料共轭物，以及这些染料在染色样品和检测配体或其它分析物中的用途。

    Derivatives of 6,8-difluoro-7-hydroxycoumarin
    6.
    发明授权
    Derivatives of 6,8-difluoro-7-hydroxycoumarin 失效
    6,8-二氟-7-羟基香豆素的衍生物

    公开(公告)号：US5830912A

    公开(公告)日：1998-11-03

    申请号：US749684

    申请日：1996-11-15

    申请人： Kyle R. Gee ,  Richard P. Haugland ,  Wei-Chuan Sun

    发明人： Kyle R. Gee ,  Richard P. Haugland ,  Wei-Chuan Sun

    IPC分类号： C07D311/16 ,  C07D311/54 ,  C07F9/655 ,  C09B57/02 ,  A61K31/37 ,  C07D311/14

    CPC分类号： C07D311/16 ,  C07D311/54 ,  C07F9/65522 ,  C09B57/02

    摘要： The present invention comprises 6,8-difluoro-7-hydroxycoumarins and derivatives of 6,8-difluoro-7-hydroxycoumarins, including reactive dyes, dye-conjugates and enzyme substrates. These fluorine-substituted fluorescent dyes typically possess greater photostability and lower pH sensitivity in the physiological pH range than their nonfluorinated analogs, exhibit less fluorescence quenching when conjugated to a substance, possess absorption and emission spectra that closely match those of their nonfluorinated analogs, and also exhibit higher quantum yields than their nonfluorinated analogs.

    摘要翻译： 本发明包括6,8-二氟-7-羟基香豆素和6,8-二氟-7-羟基香豆素的衍生物，包括活性染料，染料共轭物和酶底物。 这些氟取代的荧光染料通常在生理pH范围内比其非氟化类似物具有更高的光稳定性和较低的pH敏感性，当与物质缀合时显示较少的荧光猝灭，具有与其非氟化类似物的吸收和发射光谱非常接近的吸收和发射光谱，以及 表现出比其非氟化类似物更高的量子产率。

    Photocleavable derivatives of hydroxyprenesulfonic acids
    7.
    发明授权
    Photocleavable derivatives of hydroxyprenesulfonic acids 失效
    羟基磺酸的可透光衍生物

    公开(公告)号：US5514710A

    公开(公告)日：1996-05-07

    申请号：US180543

    申请日：1994-01-11

    申请人： Richard P. Haugland ,  Kyle R. Gee

    发明人： Richard P. Haugland ,  Kyle R. Gee

    IPC分类号： C07C309/43 ,  A61K31/265 ,  A61K31/185 ,  C07C69/96 ,  C07C309/38

    CPC分类号： C07C309/43

    摘要： The invention describes the synthesis and use of photoactivated (or caged) fluorescent dyes. Upon illumination at less than about 400 nm the caged dyes release highly fluorescent, water soluble hydroxypyrenesulfonic acid dyes according to the following equation: ##STR1## X, Y, and Z are independently sulfonic acid, a sulfonic acid salt, a hydroxyl group, or hydrogen, with at least one of X, Y, and Z being a sulfonic acid or sulfonic acid salt. LINK is either an ether linkage or a carbonate linkage. BLOCK is a caging group whose photolysis results in liberation of a free hydroxypyrenesulfonic acid dye.The caged fluorescent dyes are useful for application in aqueous solutions, including fluids of biological origin. The caged dyes of the present invention are especially useful for flow tagging velocimetry.

    摘要翻译： 本发明描述了光活化（或笼式）荧光染料的合成和应用。 在小于约400nm的照射下，笼状染料根据以下等式释放高度荧光的水溶性羟基苯磺酸染料：X，Y和Z独立地为磺酸，磺酸盐， 羟基或氢，X，Y和Z中的至少一个为磺酸或磺酸盐。 LINK是醚键或碳酸酯键。 BLOCK是一种闭孔组，其光解导致游离羟基苯磺酸染料的释放。 笼式荧光染料可用于水溶液，包括生物来源的流体。 本发明的笼状染料特别适用于流动标签测速。

    Compositions and methods for detection and isolation of phosphorylated molecules
    10.
    发明授权
    Compositions and methods for detection and isolation of phosphorylated molecules 有权
    用于磷酸化分子检测和分离的组合物和方法

    公开(公告)号：US07445894B2

    公开(公告)日：2008-11-04

    申请号：US10821522

    申请日：2004-04-09

    申请人： Brian Agnew ,  Kyle R. Gee ,  Vladimir V. Martin

    发明人： Brian Agnew ,  Kyle R. Gee ,  Vladimir V. Martin

    IPC分类号： G01N33/50

    CPC分类号： G01N33/5008 ,  G01N33/502 ,  G01N33/5091 ,  G01N2333/4709

    摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

    摘要翻译： 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。