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75 条记录 (耗时 0.031 秒)

    Derivatives of 6,8-difluoro-7-hydroxycoumarin
    2.
    发明授权
    Derivatives of 6,8-difluoro-7-hydroxycoumarin 失效
    6,8-二氟-7-羟基香豆素的衍生物

    公开(公告)号：US5830912A

    公开(公告)日：1998-11-03

    申请号：US749684

    申请日：1996-11-15

    申请人： Kyle R. Gee ,  Richard P. Haugland ,  Wei-Chuan Sun

    发明人： Kyle R. Gee ,  Richard P. Haugland ,  Wei-Chuan Sun

    IPC分类号： C07D311/16 ,  C07D311/54 ,  C07F9/655 ,  C09B57/02 ,  A61K31/37 ,  C07D311/14

    CPC分类号： C07D311/16 ,  C07D311/54 ,  C07F9/65522 ,  C09B57/02

    摘要： The present invention comprises 6,8-difluoro-7-hydroxycoumarins and derivatives of 6,8-difluoro-7-hydroxycoumarins, including reactive dyes, dye-conjugates and enzyme substrates. These fluorine-substituted fluorescent dyes typically possess greater photostability and lower pH sensitivity in the physiological pH range than their nonfluorinated analogs, exhibit less fluorescence quenching when conjugated to a substance, possess absorption and emission spectra that closely match those of their nonfluorinated analogs, and also exhibit higher quantum yields than their nonfluorinated analogs.

    摘要翻译： 本发明包括6,8-二氟-7-羟基香豆素和6,8-二氟-7-羟基香豆素的衍生物，包括活性染料，染料共轭物和酶底物。 这些氟取代的荧光染料通常在生理pH范围内比其非氟化类似物具有更高的光稳定性和较低的pH敏感性，当与物质缀合时显示较少的荧光猝灭，具有与其非氟化类似物的吸收和发射光谱非常接近的吸收和发射光谱，以及 表现出比其非氟化类似物更高的量子产率。

    Crown ether derivatives
    4.
    发明授权
    Crown ether derivatives 失效
    冠醚衍生物

    公开(公告)号：US06962992B2

    公开(公告)日：2005-11-08

    申请号：US10026302

    申请日：2001-12-19

    申请人： Vladimir V. Martin ,  Kyle R. Gee ,  Richard P. Haugland ,  Zhenjun Diwu

    发明人： Vladimir V. Martin ,  Kyle R. Gee ,  Richard P. Haugland ,  Zhenjun Diwu

    IPC分类号： C07D273/00 ,  C07H21/00 ,  C09B57/10 ,  C09B69/00 ,  G01N33/58 ,  G01N33/84 ,  C07D413/04 ,  C07D273/08 ,  C07D317/28

    CPC分类号： A61K49/0052 ,  A61K49/0021 ,  A61K49/0041 ,  C07D273/00 ,  C07H21/00 ,  C09B11/24 ,  C09B23/04 ,  C09B57/00 ,  C09B57/10 ,  C09B69/00 ,  G01N33/582 ,  G01N33/84

    摘要： The invention describes crown ether chelators, including crown ethers having the formula and aza-substituted and thia-substituted analogs thereof. These crown ethers are substituted by a dye moiety, a chemically reactive group, a conjugated substance, or a combination thereof. Chelators that are substituted by fluorescent dyes are particularly useful as indicators for metal cations, particularly Na+ and K+ ions, and particularly where binding of the target ion results in a change in the fluorescence properties of the indicator that can be correlated with the ion concentration. Methods are provided for utilizing reactive groups on the chelators for conjugation to dyes, lipids and polymers and methods for enhancing entry of the indicators into living cells.

    摘要翻译： 本发明描述了冠醚螯合剂，包括具有下式的冠醚和其氮杂取代和硫取代的类似物。 这些冠醚被染料部分，化学反应性基团，共轭物质或其组合取代。 被荧光染料取代的螯合剂特别可用作金属阳离子，特别是Na +和K + +离子的指示剂，特别是当目标离子的结合导致变化时 在可以与离子浓度相关的指示剂的荧光特性中。 提供了利用螯合剂上的反应性基团与染料偶联的方法，脂质和聚合物以及用于增强指示剂进入活细胞的方法。

    Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
    5.
    发明授权
    Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings 有权
    含稠环的1,2-二氢-7-羟基喹啉的衍生物

    公开(公告)号：US06716979B2

    公开(公告)日：2004-04-06

    申请号：US09922333

    申请日：2001-08-04

    申请人： Zhenjun Diwu ,  Jiaxing Liu ,  Richard P. Haugland ,  Kyle R. Gee

    发明人： Zhenjun Diwu ,  Jiaxing Liu ,  Richard P. Haugland ,  Kyle R. Gee

    IPC分类号： C07D49122

    CPC分类号： C07D471/04 ,  C07D491/04 ,  C07D495/04 ,  C07D495/14 ,  G01N33/582 ,  Y10S436/80

    摘要： The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.

    摘要翻译： 本发明描述了新颖的染料，包括掺入另外的稠合芳环的香豆素，罗丹明和罗丹醇。 本发明的染料比不具有稠合芳环的结构相似的染料长的波长吸收。 本发明的许多染料是有用的荧光染料。 本发明包括化学活性染料，染料共轭物，以及这些染料在染色样品和检测配体或其它分析物中的用途。

    Caged nucleotides
    6.
    发明授权
    Caged nucleotides 失效
    笼状核苷酸

    公开(公告)号：US5872243A

    公开(公告)日：1999-02-16

    申请号：US497183

    申请日：1995-06-30

    申请人： Kyle R. Gee ,  Hon Cheung Lee ,  Robert Aarhus ,  Richard P. Haugland ,  Timothy F. Walseth ,  Richard M. Graeff

    发明人： Kyle R. Gee ,  Hon Cheung Lee ,  Robert Aarhus ,  Richard P. Haugland ,  Timothy F. Walseth ,  Richard M. Graeff

    IPC分类号： C07H19/20 ,  C07H21/02 ,  C07H1/00 ,  C07H21/00

    CPC分类号： C07H19/20 ,  C07H21/02

    摘要： The present invention describes a family of photolabile caged nucleotides, including cyclic nucleotides. The compounds of the present invention are caged analogs and derivatives of NAD.sup.+, NADH, NADP, NADPH, NAADP and cADPR. The photolysis of the present compounds allows the release of the free nucleotide in vivo or in vitro with precise spatial and temporal control. The compounds are useful for the photolytic generation of free nucleotides in aqueous samples, for example, in the study of calcium mobilization in cells and cell homogenates.

    摘要翻译： 本发明描述了一种包含环状核苷酸的光不稳定笼状核苷酸家族。 本发明的化合物是NAD +，NADH，NADP，NADPH，NAADP和cADPR的笼养类似物和衍生物。 本发明化合物的光解使得体内或体外释放游离核苷酸具有精确的空间和时间控制。 这些化合物可用于水溶液样品中游离核苷酸的光解产生，例如在细胞和细胞匀浆中钙迁移的研究中。

    Photocleavable derivatives of hydroxyprenesulfonic acids
    7.
    发明授权
    Photocleavable derivatives of hydroxyprenesulfonic acids 失效
    羟基磺酸的可透光衍生物

    公开(公告)号：US5514710A

    公开(公告)日：1996-05-07

    申请号：US180543

    申请日：1994-01-11

    申请人： Richard P. Haugland ,  Kyle R. Gee

    发明人： Richard P. Haugland ,  Kyle R. Gee

    IPC分类号： C07C309/43 ,  A61K31/265 ,  A61K31/185 ,  C07C69/96 ,  C07C309/38

    CPC分类号： C07C309/43

    摘要： The invention describes the synthesis and use of photoactivated (or caged) fluorescent dyes. Upon illumination at less than about 400 nm the caged dyes release highly fluorescent, water soluble hydroxypyrenesulfonic acid dyes according to the following equation: ##STR1## X, Y, and Z are independently sulfonic acid, a sulfonic acid salt, a hydroxyl group, or hydrogen, with at least one of X, Y, and Z being a sulfonic acid or sulfonic acid salt. LINK is either an ether linkage or a carbonate linkage. BLOCK is a caging group whose photolysis results in liberation of a free hydroxypyrenesulfonic acid dye.The caged fluorescent dyes are useful for application in aqueous solutions, including fluids of biological origin. The caged dyes of the present invention are especially useful for flow tagging velocimetry.

    摘要翻译： 本发明描述了光活化（或笼式）荧光染料的合成和应用。 在小于约400nm的照射下，笼状染料根据以下等式释放高度荧光的水溶性羟基苯磺酸染料：X，Y和Z独立地为磺酸，磺酸盐， 羟基或氢，X，Y和Z中的至少一个为磺酸或磺酸盐。 LINK是醚键或碳酸酯键。 BLOCK是一种闭孔组，其光解导致游离羟基苯磺酸染料的释放。 笼式荧光染料可用于水溶液，包括生物来源的流体。 本发明的笼状染料特别适用于流动标签测速。

    Antibody complexes and methods for immunolabeling
    8.
    发明授权
    Antibody complexes and methods for immunolabeling 有权
    抗体复合物和免疫标记方法

    公开(公告)号：US08323903B2

    公开(公告)日：2012-12-04

    申请号：US10118204

    申请日：2002-04-05

    申请人： Robert A. Archer ,  Joseph M. Beechem ,  David C. Hagen ,  Richard P. Haugland ,  Rosaria P. Haugland

    发明人： Robert A. Archer ,  Joseph M. Beechem ,  David C. Hagen ,  Richard P. Haugland ,  Rosaria P. Haugland

    IPC分类号： G01N33/53

    CPC分类号： B82Y30/00 ,  B82Y5/00 ,  B82Y10/00 ,  G01N33/53 ,  G01N33/58 ,  G01N33/6857

    摘要： The present invention provides novel immunolabeling complexes and certain components of such complexes, as well as methods of preparing and using such complexes, and kits for use in preparing labeling proteins and for immunolabeling. The pre-formed immunolabeling complexes of the invention comprise both a target-binding antibody and a labeling protein that contains covalently attached labels, where the labeling protein binds selectively and with high affinity to a selected region of the target-binding antibody. Novel labeling proteins of the invention include non-antibody peptides and proteins, such as a complex of protein G and a labeled albumin, and monovalent antibody fragments, such as labeled Fab fragments of an anti-Fc antibody. In methods of the invention, the preformed immunolabeling complexes are added to the sample alone or in combination, for purposes of labeling and optionally detecting the target of interest.

    摘要翻译： 本发明提供了新的免疫标记复合物和这些复合物的某些成分，以及制备和使用这些复合物的方法，以及用于制备标记蛋白和免疫标记的试剂盒。 本发明的预先形成的免疫粘附复合物包含靶结合抗体和含有共价连接的标记的标记蛋白，其中所述标记蛋白选择性地结合并且与靶结合抗体的选定区域具有高亲和力。 本发明的新型标记蛋白包括非抗体肽和蛋白质，例如蛋白G和标记的白蛋白的复合物，以及单价抗体片段，例如抗Fc抗体的标记的Fab片段。 在本发明的方法中，为了标记和任选地检测目的靶标，将预先形成的免疫标记复合物单独或组合加入到样品中。

    Aza-benzazolium containing cyanine dyes
    9.
    发明授权
    Aza-benzazolium containing cyanine dyes 有权
    含有氰基染料的氮杂 -

    公开(公告)号：US07226740B2

    公开(公告)日：2007-06-05

    申请号：US10683753

    申请日：2003-10-13

    申请人： Richard P. Haugland ,  Stephen T. Yue

    发明人： Richard P. Haugland ,  Stephen T. Yue

    IPC分类号： C12Q1/68 ,  C07D215/00 ,  C07D215/12

    CPC分类号： C09B23/02 ,  C12Q1/6816 ,  C12Q1/6876 ,  G01N1/30 ,  G01N27/44726 ,  G01N33/52 ,  G01N33/5308 ,  G01N33/531 ,  G01N33/582 ,  G01N33/68 ,  G01N2001/302 ,  Y10S436/80 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2563/107

    摘要： Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.

    摘要翻译： 描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料，包括由阳离子侧链取代的花青染料，单体和二聚花青染料，化学反应性花青染料和花青染料的缀合物。 当在水溶液中稀释时，受试染料实际上是非荧光的，但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时，它们显示出明亮的荧光。 描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用，包括溶液，电泳凝胶，细胞和微生物。

    Detection of phosphate using coupled enzymatic reactions
    10.
    发明授权
    Detection of phosphate using coupled enzymatic reactions 有权
    使用偶联酶反应检测磷酸盐

    公开(公告)号：US06265179B1

    公开(公告)日：2001-07-24

    申请号：US09495882

    申请日：2000-02-01

    申请人： Mingjie Zhou ,  Richard P. Haugland

    发明人： Mingjie Zhou ,  Richard P. Haugland

    IPC分类号： C12Q128

    CPC分类号： C12Q1/26 ,  C12Q1/42 ,  C12Q1/48 ,  G01N2333/91102 ,  G01N2333/91142 ,  G01N2333/916 ,  Y10S435/968 ,  Y10S435/975

    摘要： Inorganic phosphate may be detected and optionally quantified via the coupling of a phosphate-dependent enzymatic reaction with an enzyme system that generates hydrogen peroxide in the presence of a chromogenic or fluorogenic peroxidase substrate. Phosphate consuming or phosphate-producing enzymes or their substrates may also be detected and/or quantified, including pyrophosphatase enzymes or pyrophosphatase.

    摘要翻译： 可以通过磷酸盐依赖性酶反应与在显色或荧光过氧化物酶底物存在下产生过氧化氢的酶系统的偶联来检测和任选地定量无机磷酸盐。 磷酸盐消耗或磷酸盐生成酶或其底物也可被检测和/或定量，包括焦磷酸酶或焦磷酸酶。