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1.发明授权公开(公告)号：US08512959B2
公开(公告)日：2013-08-20
申请号：US10588392
申请日：2005-02-09
申请人： Laszlo Takacs , Andras Guttman , William S. Hancock , Barry L. Karger , Manuel Duval , Patrick Berna
发明人： Laszlo Takacs , Andras Guttman , William S. Hancock , Barry L. Karger , Manuel Duval , Patrick Berna
IPC分类号： G01N33/53
CPC分类号： G01N33/6854 , B01L3/5027 , C07K16/18 , G01N33/54386 , G01N33/577 , G01N33/6845 , G01N2500/00
摘要： A method or platform for monoclonal antibody based biomarker discovery is disclosed. The method according to the invention provides for the integration of analyte collection, hybridoma screening and nanovolume integrated mass spectrometry (NVIMS) to achieve a robust screening system that is capable, for example, of cutting 4-6 years off of the classical biomarker discovery and development process. The invention provides a platform for the rapid, high-throughput production, isolation and characterization of, e.g., disease specific biomarkers together with highly specific monoclonal antibodies. The method of the invention has a variety of applications such as, but not limited to, drug testing, biohazard applications, ecological applications, physiological applications and/or pathology screening applications. The method of the invention is also capable of being performed or used as or with a high-throughput screening process or system of the invention.
摘要翻译： 公开了用于基于单克隆抗体的生物标志物发现的方法或平台。 根据本发明的方法提供了分析物收集，杂交瘤筛选和纳瓦级综合质谱（NVIMS）的整合，以实现强大的筛选系统，其能够例如从经典生物标志物发现开始4-6年， 开发过程。 本发明提供了用于快速，高通量生产，分离和表征例如疾病特异性生物标志物以及高度特异性单克隆抗体的平台。 本发明的方法具有多种应用，例如但不限于药物测试，生物危害应用，生态应用，生理应用和/或病理学筛选应用。 本发明的方法还能够作为或与本发明的高通量筛选方法或系统一起使用或使用。
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2.发明申请Proteomic Methods For The Identification And Use Of Putative Biomarkers Associated With The Dysplastic State In Cervical Cells Or Other Cell Types 审中-公开用于鉴定和使用与宫颈细胞或其他细胞类型中的发育不良状态相关的推定生物标志物的蛋白质组学方法公开(公告)号：US20090221430A1
公开(公告)日：2009-09-03
申请号：US12224973
申请日：2007-03-12
IPC分类号： C40B30/00 , C12Q1/68 , G01N33/68 , G01N33/536 , C12Q1/02
CPC分类号： G01N33/57411
摘要： The invention relates to methods for detecting and identifying potential biomarkers of high-grade cervical dysplasia in an individual human subject. The invention also relates to newly discovered biomarkers, as set forth in Tables 1-4 herein, which are associated with the dysplastic state of cervical cells. It has been discovered that a differential level of expression of any of these markers or combination of these markers correlates with a dysplastic condition in a human subject, e.g., a patient.
摘要翻译： 本发明涉及用于检测和鉴定个体人类受试者高级宫颈发育不良的潜在生物标志物的方法。 本发明还涉及新发现的与本发明的表1-4所示的生物标志物，其与子宫颈细胞的发育不良状态相关。 已经发现，这些标记物或这些标记物的组合的表达差异水平与人受试者例如患者中的发育异常状况相关。
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公开(公告)号：US20080280317A1
公开(公告)日：2008-11-13
申请号：US11661525
申请日：2005-08-29
CPC分类号： G01N33/6848 , G01N33/6842
摘要： A combination of “bottom up” and “top down” MS analysis of posttranslational modifications in complex proteins is described. The method comprises digestion of the protein with an enzyme that forms larger peptide fragments than trypsin (>3000 D), performing HPLC with the fragments and applying a new data acquisition strategy using on-line coupling with e.g. LTQ-FTMS, a hybrid mass spectrometer that couples a linear ion trap with a Fourier transform ion cyclotron resonance (FTICR) cell. The method is applied to analysis of posttranslational modifications of protein isoforms.
摘要翻译： 描述了复合蛋白中翻译后修饰的“自下而上”和“自顶向下”MS分析的组合。 该方法包括用比胰蛋白酶（> 3000D）形成更大的肽片段的酶消化蛋白质，用片段进行HPLC，并使用在线偶联方法应用新的数据采集策略。 LTQ-FTMS是将线性离子阱与傅里叶变换离子回旋共振（FTICR）电池耦合的混合质谱仪。 该方法适用于蛋白质同工型翻译后修饰的分析。
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公开(公告)号：US08628912B2
公开(公告)日：2014-01-14
申请号：US12667456
申请日：2008-07-03
IPC分类号： A01N1/02
CPC分类号： G01N33/74 , G01N33/6848 , G01N33/92 , G01N2800/042 , G01N2800/044 , G01N2800/321 , G01N2800/322 , G01N2800/50
摘要： Methods of identifying subjects having, or at risk of developing, diabetes, obesity, and/or hypertension are disclosed, as well as methods of identifying biomarkers for diabetes, obesity, and/or hypertension, and biomarkers identified by such methods.
摘要翻译： 公开了识别具有发生糖尿病，肥胖和/或高血压的风险的受试者的方法，以及鉴定糖尿病，肥胖症和/或高血压的生物标志物的方法以及通过这些方法鉴定的生物标志物的方法。
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公开(公告)号：US20090166224A1
公开(公告)日：2009-07-02
申请号：US11579610
申请日：2005-05-05
申请人： Ziping Yang , William S. Hancock , Marina Hincapie
发明人： Ziping Yang , William S. Hancock , Marina Hincapie
IPC分类号： G01N27/26 , C07K1/22 , C07K14/00 , C07K1/00 , G01N33/68 , G01N33/566 , C12Q1/02 , G01N33/53 , B01D59/44
CPC分类号： G01N33/6842 , C07K1/22 , G01N2333/4724 , G01N2400/00
摘要： Methods, compositions, and kits related to the use of multi-ligand affinity chromatography are described. The methods include those related to identification of glycoprotein panels for analyzing and diagnosing disease.
摘要翻译： 描述了与使用多配体亲和层析相关的方法，组合物和试剂盒。 这些方法包括与识别用于分析和诊断疾病的糖蛋白板相关的方法。
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6.发明授权Method of binding a plurality of chemicals on a substrate by electrophoretic self-assembly 失效通过电泳自组装将基板上的多种化学物质结合的方法
公开(公告)号：US6107038A
公开(公告)日：2000-08-22
申请号：US134855
申请日：1998-08-14
申请人： Gargi Choudhary , Karen Hahnenberger , Philip J. Kuekes , Kay Lichtenwalter , William S. Hancock
发明人： Gargi Choudhary , Karen Hahnenberger , Philip J. Kuekes , Kay Lichtenwalter , William S. Hancock
IPC分类号： B01J19/00 , G01N27/447 , G11C13/02 , C12Q1/68
CPC分类号： G11C13/0019 , B01J19/0046 , B82Y10/00 , B82Y15/00 , B82Y30/00 , B82Y5/00 , G01N27/447 , G11C13/0014 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00657 , B01J2219/00659 , B01J2219/00713 , Y10T436/143333
摘要： An electrophoretic technique is provided for moving a plurality of chemicals into distinct zones for immobilization on a solid surface. The technique includes introducing a first electrolyte and a second electrolyte into a channel, and interposing between the first and second electrolytes at least one solution containing a plurality of chemicals. Under a given electric field, the first electrolyte has anions with higher effective mobility than the chemicals and the second electrolyte has anions with lower effective mobility than the chemicals. When an electrical potential is applied across the length of the channel the plurality of chemicals in the solution are moved into spatial zones. The chemicals in the zones can then be bound to the interior surface of the channel. Chemicals so bound to the wall surface can be used to build very small structures such as arrays and electrical conducting structures.
摘要翻译： 提供了一种电泳技术，用于将多种化学品移动到不同的区域中，以固定在固体表面上。 该技术包括将第一电解质和第二电解质引入通道中，并且在第一和第二电解质之间插入至少一种含有多种化学物质的溶液。 在给定的电场下，第一电解质具有比化学品更高的有效迁移率的阴离子，并且第二电解质具有比化学品更低的有效迁移率的阴离子。 当跨越通道的长度施加电位时，溶液中的多种化学物质移动到空间区域中。 然后，区域中的化学物质可以结合到通道的内表面。 可以使用与壁表面结合的化学物质来构建非常小的结构，例如阵列和导电结构。
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公开(公告)号：US4603010A
公开(公告)日：1986-07-29
申请号：US736306
申请日：1985-05-21
IPC分类号： C07K14/00 , A61K35/14 , A61K35/16 , A61K38/16 , A61M1/36 , B01J39/04 , B01J47/00 , C07K15/16
CPC分类号： B01J47/003 , A61K35/16 , B01J39/043
摘要： A process is described for removing low density and very low density lipoproteins from blood plasma or serum and recovering the plasma or serum in a physiologically acceptable form. The method involves in a preferred embodiment passing plasma or serum through a cationic ion exchanger equilibrated with a physiologically acceptable saline solution. The ion exchanger is a water insoluble hydrophilic, water swellable cross-linked regenerated or microgranular cellulose matrix substituted with hydroxy C.sub.2 -C.sub.4 alkyl group. The ion exchanger capacity is provided by sulphate groups substituted with from 2 to 6 meq/g. The take up of low density lipoproteins is enhanced by recycling the plasma through the column.
摘要翻译： 描述了从血浆或血清中除去低密度和极低密度脂蛋白的方法，并以生理上可接受的形式回收血浆或血清。 该方法包括在优选实施方案中使血浆或血清通过用生理学上可接受的盐水溶液平衡的阳离子离子交换剂。 离子交换剂是由羟基C 2 -C 4烷基取代的水不溶性亲水性，水溶胀性交联的再生或微粒状纤维素基质。 离子交换容量由2〜6meq / g取代的硫酸盐基团提供。 低密度脂蛋白的吸收通过将血浆循环通过柱来增强。
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公开(公告)号：US5716825A
公开(公告)日：1998-02-10
申请号：US551501
申请日：1995-11-01
CPC分类号： H01J49/0418 , B01J19/0093 , C12Q1/6816 , B01J2219/00828 , B01J2219/00831 , B01J2219/00833 , B01J2219/00835 , B01J2219/00873 , B01J2219/00889 , B01J2219/00891 , B01J2219/00952 , B01J2219/00961
摘要： An integrated nucleic acid sample analysis system for matrix-assisted laser-desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS) is described. The integrated system comprises a miniaturized sample preparation compartment interfaced directly with a MALDI-TOF ionization surface for amplifying and/or otherwise chemically manipulating an oligonucleotide analyte and presenting the analyte to a MALDI ionization surface for mass spectrometry analysis. The miniaturized integrated sample handling system disclosed herein finds application in the amplification and analysis of DNA samples for genetic diagnosis and other uses.
摘要翻译： 描述了用于基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）的综合核酸样品分析系统。 集成系统包括与MALDI-TOF电离表面直接接合的小型化样品制备室，用于放大和/或以其它方式化学操作寡核苷酸分析物，并将分析物呈现到MALDI电离表面用于质谱分析。 本文公开的小型化综合样品处理系统可用于扩增和分析用于遗传诊断和其它用途的DNA样品。
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公开(公告)号：US5130255A
公开(公告)日：1992-07-14
申请号：US627287
申请日：1990-12-13
CPC分类号： G01N33/68 , Y10T436/12 , Y10T436/200833
摘要： Container systems for stable storage of pharmaceutical compounds are disclosed. The container systems prevent the formation of adducts between pharmaceutical compounds and aldehydes during storage of these compounds in containers sealed with elastomeric stoppers that leach aldehydes. Methods for determining the presence of these adducts are also disclosed.
摘要翻译： 公开了用于药物化合物稳定储存的容器系统。 在将这些化合物储存在用浸出醛的弹性塞子密封的容器中时，容器系统防止在药物化合物和醛之间形成加合物。 还公开了确定这些加合物的存在的方法。
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公开(公告)号：US4330440A
公开(公告)日：1982-05-18
申请号：US128847
申请日：1980-03-10
IPC分类号： C08B15/06 , C08B37/00 , C12N11/10 , G01N33/543 , G01N33/548 , C08L1/00
CPC分类号： G01N33/548 , C08B15/06 , C08B37/0039 , C12N11/10 , G01N33/54353
摘要： The invention relates to the carbonylation of cross linked substituted and unsubstituted polysaccharides, their copolymers with macroporous synthetic polymers, macroporous synthetic polymers and rigid supports with pendant hydroxyalkyl groups. The carbonylated product can be used to prepare an affinity chromatography matrix which remains an uncharged species at varying pH's. The carbonylated product is also useful for the preparation of other compounds: Typical polysaccharides are agarose, starch, dextran, cellulose and regenerated cellulose, typical macroporous synthetic polymers are acrylamides, acrylates and methacrylates, typical rigid supports are silica beads coated with hydroxy alkyl groups and typical cabonylating agents are N,N'-carbonyl diimidazole; N,N'carbonyl di-1,2,3-benzotriazole; and N,N'-carbonyl di-1,2,4-triazole.
摘要翻译： 本发明涉及交联的取代和未取代的多糖，它们与大孔合成聚合物的共聚物，大孔合成聚合物和具有侧羟基烷基的刚性载体的羰基化。 羰基化产物可用于制备在不同pH值下保持不带电荷的物质的亲和层析基质。 羰基化产物也可用于制备其他化合物：典型的多糖是琼脂糖，淀粉，葡聚糖，纤维素和再生纤维素，典型的大孔合成聚合物是丙烯酰胺，丙烯酸酯和甲基丙烯酸酯，典型的刚性载体是用羟基烷基涂覆的二氧化硅珠， 典型的卡罗胺化剂是N，N'-羰基二咪唑; N，N'-羰基二-1,2,3-苯并三唑; 和N，N'-羰基二-1,2,4-三唑。
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