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34 条记录 (耗时 0.029 秒)

    Method for producing α-santalene
    7.
    发明授权
    Method for producing α-santalene 有权
    α-檀香草的制备方法

    公开(公告)号：US09297004B2

    公开(公告)日：2016-03-29

    申请号：US12918140

    申请日：2009-03-04

    申请人： Michel Schalk

    发明人： Michel Schalk

    IPC分类号： C12N15/29 ,  C12N15/60 ,  C12N15/82 ,  C12P15/00 ,  C12N9/88 ,  C12P5/00

    CPC分类号： C12N9/88 ,  C12P5/007 ,  C12P15/00 ,  C12Y402/03082

    摘要： The present invention provides a method of producing α-santalene by contacting at least one polypeptide with farnesyl phyrophosphate (fpp). In particular, the method may be carried out in vitro or in vivo to produce α-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing the nucleic acid represent part of the present invention. A non-human host organism and a cell transformed to be used in the method of producing α-santalene are also part of the present invention.

    摘要翻译： 本发明提供了通过使至少一种多肽与法呢基磷酸（fpp）接触来生产α-檀香草的方法。 特别地，该方法可以在体外或体内进行以产生α-香豆素，在香料和香料领域中非常有用的化合物。 本发明还提供了可用于本发明方法的多肽的氨基酸序列。 编码本发明多肽的核酸和含有核酸的表达载体代表本发明的一部分。 非人宿主生物体和转化用于生产α-檀香烃的方法的细胞也是本发明的一部分。

    METHOD FOR PRODUCING SCLAREOL
    8.
    发明申请
    METHOD FOR PRODUCING SCLAREOL 有权
    生产SCLAREOL的方法

    公开(公告)号：US20110041218A1

    公开(公告)日：2011-02-17

    申请号：US12867861

    申请日：2009-02-12

    申请人： Michel Schalk

    发明人： Michel Schalk

    IPC分类号： C12P7/02 ,  C12N9/16 ,  C07H21/00 ,  C12N15/63 ,  C12N5/10 ,  C12N1/21 ,  C12N1/19 ,  A01H5/00

    CPC分类号： C12P7/18 ,  C07D307/92 ,  C07D311/92 ,  C07K2319/00 ,  C12N9/88

    摘要： The present invention provides a method of producing sclareol, said method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, said method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing said nucleic acid, as well as a non-human host organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.

    摘要翻译： 本发明提供了生产香紫苏醇的方法，所述方法包括将具有香紫苏醇合成酶活性的特定多肽与二硫代二磷酸酯（LPP）接触。 特别地，所述方法可以在体外或体内进行以产生香紫苏醇，香料和香料领域中非常有用的化合物。 本发明还提供了该方法中使用的多肽的氨基酸序列。 衍生自丹参的核酸并编码本发明的多肽，含有所述核酸的表达载体以及非人宿主生物或转化成携带相同核酸的细胞也是本发明的一部分 。

    Transformed plants accumulating mono-and/or sesquiterpenes
    9.
    发明申请
    Transformed plants accumulating mono-and/or sesquiterpenes 审中-公开
    积累单和/或倍半萜的转化植物

    公开(公告)号：US20080201796A1

    公开(公告)日：2008-08-21

    申请号：US11916272

    申请日：2006-06-26

    申请人： Joe Chappell ,  Shuiqin Wu ,  Michel Schalk ,  Athony Clark

    发明人： Joe Chappell ,  Shuiqin Wu ,  Michel Schalk ,  Athony Clark

    IPC分类号： A01H1/00 ,  A01H5/00 ,  C12N15/82 ,  C12N15/00

    CPC分类号： C12N15/8243 ,  C12N9/0006 ,  C12N9/1085

    摘要： The present invention relates to plant expressing transgenes and to plants transformed to comprise additional copies of genes, said genes encoding at least a HMGR-CoA reductase and a terpene synthase. The invention further claims methods for preparing the plants, and a method for producing terpenes. The present thus provides a reliable and cost effective platform for generating any terpene, in particular any mono- and/or sesquiterpene of interest. For example, the skilled person may use any gene encoding a sesquiterpene synthase for accumulating the respective sesquiterpene in the plant of the present invention.

    摘要翻译： 本发明涉及表达转基因的植物和转化成包含编码另外的基因拷贝的植物，所述基因编码至少HMGR-CoA还原酶和萜烯合酶。 本发明进一步要求制备植物的方法以及萜烯的制备方法。 因此，本发明提供了用于产生任何萜烯，特别是感兴趣的任何单 - 和/或倍半萜的可靠和具有成本效益的平台。 例如，技术人员可以使用编码倍半萜合酶的任何基因来在本发明的植物中积累相应的倍半萜烯。

    Recording of DNA sequences to enable them to be expressed in yeasts, and the transformed yeasts obtained
    10.
    发明授权
    Recording of DNA sequences to enable them to be expressed in yeasts, and the transformed yeasts obtained 失效
    记录DNA序列以使其能够在酵母中表达，并获得转化的酵母

    公开(公告)号：US06787337B1

    公开(公告)日：2004-09-07

    申请号：US09713794

    申请日：2000-11-15

    申请人： Yannick Batard ,  Francis Durst ,  Michel Schalk ,  Daniele Werck-Reichhart

    发明人： Yannick Batard ,  Francis Durst ,  Michel Schalk ,  Daniele Werck-Reichhart

    IPC分类号： C12P2100

    CPC分类号： C12N9/0042 ,  C12N15/67 ,  C12N15/81

    摘要： The present invention relates to a recombinant non-yeast DNA, which encodes a protein of interest, wherein an unmodified DNA corresponding to the recombinant non-yeast DNA contains a region having a high content of codons that are poorly suited to yeasts, wherein a number of the codons that are poorly suited to yeasts are replaced in said region of the recombinant non-yeast DNA with synonymous codons coding for the same amino acid that are well-suited to yeasts, and wherein the number of replaced codons is sufficient to permit expression in yeasts. The present invention also relates to DNA sequences which originate from dicotyledonous or monocotyledonous plants, and in particular plants of the graminae family which are selected from among wheat, barley, oats, rice, maize, sorghum and cane sugar, as well as to vectors and transformed yeasts which contain the DNA sequences of the invention.

    摘要翻译： 本发明涉及编码感兴趣的蛋白质的重组非酵母DNA，其中对应于重组非酵母DNA的未修饰的DNA含有具有高度不适于酵母的密码子的高含量区域，其中数目 的不适于酵母的密码子在所述重组非酵母DNA的所述区域中被替换，其具有编码相当于酵母的相同氨基酸的同义密码子，并且其中替换的密码子的数目足以允许表达 在酵母中 本发明还涉及源自双子叶植物或单子叶植物的DNA序列，特别是选自小麦，大麦，燕麦，稻，玉米，高粱和蔗糖的格氏族的植物，以及载体和 转化酵母含有本发明的DNA序列。