Mutant aspartokinase gene
    1.
    发明授权
    Mutant aspartokinase gene 失效
    突变型天冬氨酸激酶基因

    公开(公告)号:US5688671A

    公开(公告)日:1997-11-18

    申请号:US532828

    申请日:1995-10-27

    CPC分类号: C12N9/1217 C12N9/12 C12P13/08

    摘要: Recombinant DNA replicable in microorganisms belonging to the genus Corynebacterium, which contains a DNA fragment coding for an aspartokinase .alpha.-subunit protein originating from a bacterium belonging to the genus Corynebacterium, in which synergistic feedback inhibition by L-lysine and L-threonine is substantially desensitized, and a DNA fragment coding for an aspartokinase .beta.-subunit protein originating from a bacterium belonging to the genus Corynebacterium, in which synergistic feedback inhibition by L-lysine and L-threonine is substantially desensitized, is introduced into a microorganism belonging to the genus Corynebacterium. Thus a transformant having enhanced production and excretion speeds of L-lysine is obtained. The transformant is cultivated in an appropriate medium, and produced L-lysine is separated.

    摘要翻译: PCT No.PCT / JP93 / 01809 Sec。 371 1995年10月27日第 102(e)日期1995年10月27日PCT提交1993年12月14日PCT公布。 出版物WO94 / 25605 日期1994年11月10日在棒状杆菌属的微生物中可复制的重组DNA,其含有编码源自属于棒状杆菌属的细菌的天冬氨酸激酶α亚基蛋白的DNA片段,其中L-赖氨酸和L的协同反馈抑制 - 苏氨酸基本脱敏,编码源于属于棒状杆菌属的细菌的天冬氨酸激酶β亚基蛋白的DNA片段,其中L-赖氨酸和L-苏氨酸的协同反馈抑制基本上脱敏,被引入微生物 属于棒杆菌属。 因此获得具有增强的L-赖氨酸的产生和排泄速度的转化体。 将转化体在合适的培养基中培养,分离出L-赖氨酸。

    Method of producing L-lysine
    2.
    发明授权
    Method of producing L-lysine 失效
    生产L-赖氨酸的方法

    公开(公告)号:US5766925A

    公开(公告)日:1998-06-16

    申请号:US700359

    申请日:1996-10-08

    摘要: A coryneform bacterium having high L-lysine productivity is provided by integrating a gene coding for aspartokinase originating from coryneform bacteria with desensitized feedback inhibition by L-lysine and L-threonine, into chromosomal DNA of a coryneform bacterium harboring leaky type homoserine dehydrogenase or a coryneform bacterium deficient in homoserine dehydrogenase gene.

    摘要翻译: PCT No.PCT / JP95 / 00268 Sec。 371日期1996年10月8日第 102(e)日期1996年10月8日PCT提交1995年2月23日PCT公布。 出版物WO95 / 23864 日期1995年9月8日具有高L-赖氨酸生产力的棒状细菌通过将编码来自棒状细菌的天冬氨酸激酶的基因与L-赖氨酸和L-苏氨酸的脱敏反馈抑制基因整合到含有漏型的棒状杆菌型细菌的染色体DNA中来提供 高丝氨酸脱氢酶或缺失高丝氨酸脱氢酶基因的棒状细菌。

    Method for producing target substance by fermentation
    5.
    发明授权
    Method for producing target substance by fermentation 有权
    通过发酵生产目标物质的方法

    公开(公告)号:US08497087B2

    公开(公告)日:2013-07-30

    申请号:US11275900

    申请日:2006-02-02

    摘要: A method for producing a target substance by utilizing a microorganism by culturing the microorganism in a medium to produce and accumulate the target substance in the medium and collecting the target substance from the culture is described. The microorganism is a mutant recombinant strain in which maltose assimilation is controlled by reducing or eliminating the interaction between IIAGlc protein of the glucose PTS and a protein involved in non-PTS uptake of maltose.

    摘要翻译: 描述了通过在培养基中培养微生物来生产和积累目标物质并从培养物中收集目标物质的微生物生产目标物质的方法。 该微生物是通过减少或消除葡萄糖PTS的IIAGlc蛋白与参与非PTS摄取麦芽糖的蛋白质之间的相互作用来控制麦芽糖同化的突变重组菌株。

    Bacteria containing aspartate-semialdehyde dehydrogenase, phosphoenolpyruvate carboxylase, and transhydrogenase to produce L-lysine in escherichia, and methods of using same
    6.
    发明授权
    Bacteria containing aspartate-semialdehyde dehydrogenase, phosphoenolpyruvate carboxylase, and transhydrogenase to produce L-lysine in escherichia, and methods of using same 有权
    含有天冬氨酸 - 半醛脱氢酶,磷酸烯醇丙酮酸羧化酶和转氢酶在埃希氏菌中产生L-赖氨酸的细菌及其使用方法

    公开(公告)号:US07723081B1

    公开(公告)日:2010-05-25

    申请号:US10149450

    申请日:2000-01-21

    IPC分类号: C12P13/08

    CPC分类号: C12P13/08 C12N15/52

    摘要: An Escherichia bacterium (1) which harbors dihydrodipicolinate synthase of which feedback inhibition by L-lysine is desensitized and aspartokinase of which feedback inhibition by L-lysine is desensitized, (2) in which intracellular activity of dihydrodipicolinate reductase is enhanced, and (3) in which a diaminopimelate dehydrogenase gene is introduced or intracellular activities of tetrahydrodipicolinate succinylase and succinyl diaminopimelate deacylase are enhanced, wherein intracellular activity of aspartate-semialdehyde dehydrogenase or phosphoenolpyruvate carboxylase is enhanced, is cultured in a suitable medium to produce and accumulate L-lysine in culture, and the L-lysine is collected from the culture.

    摘要翻译: 具有L-赖氨酸的反馈抑制脱敏的二氢吡啶并吡啶合酶的大肠杆菌细菌(1),L-赖氨酸的反馈抑制的天冬氨酸脱敏,(2)二氢吡啶二羧酸还原酶的细胞内活性增强,(3) 其中引入二氨基庚二酸脱氢酶基因或增加四氢二吡啶甲酰基琥珀酰基酶和琥珀酰二氨基庚二酸脱酰基酶的细胞内活性,其中天冬氨酸半醛脱氢酶或磷酸烯醇丙酮酸羧化酶的细胞内活性增强,在合适的培养基中培养以在培养物中产生和积累L-赖氨酸 ,并从培养物中收集L-赖氨酸。

    Zero thermal expansion material and practical component parts making use of the same
    9.
    发明授权
    Zero thermal expansion material and practical component parts making use of the same 有权
    零热膨胀材料和实用组件使用相同

    公开(公告)号:US06844283B2

    公开(公告)日:2005-01-18

    申请号:US10837718

    申请日:2004-05-04

    摘要: Using as a negative thermal expansion material a double oxide containing at least partly a compound represented by the chemical formula: RQ,O, (wherein R is Zr, Hf or a tetravalent metallic element represented by a mixture system of these, and Q is a hexavalent metallic element selected from W and Mo), and using as a positive thermal expansion material a material containing at least partly a compound represented by the chemical formula: MQX, (wherein M is Mg, Ca, Sr, Ba, Ra or a divalent metallic element represented by a mixture system of any of these, Q is a hexavalent metallic element selected from W and Mo, and X is an element selected from O and S), these are mixed preferably in a weight ratio of 1:1 and are synthesized to obtain a material whose coefficient of thermal expansion is substantially zero over a wide temperature range, i.e., a zero thermal expansion material. Using this zero thermal expansion material, high-precision and high-performance practical component parts can be obtained.

    摘要翻译: 使用至少部分由化学式RQ2O8表示的化合物(其中R是Zr,Hf或由它们的混合体系表示的四价金属元素)的双氧化物作为负热膨胀材料,Q是六价金属元素 选自W和Mo),并且使用至少部分由化学式MQX4表示的化合物(其中M为Mg,Ca,Sr,Ba,Ra或由下式表示的二价金属元素作为正热膨胀材料) 任何这些的混合体系,Q是选自W和Mo的六价金属元素,X是选自O和S的元素),优选以1:1的重量比混合,得到 热膨胀系数在宽温度范围内基本为零的材料,即零热膨胀材料。 使用这种零热膨胀材料,可以获得高精度和高性能的实用部件。