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    LIQUID SUPPLY DRIVE MECHANISM USING OSMOTIC PUMP AND MICROCHIP HAVING THE LIQUID SUPPLY DRIVE MECHANISM
    9.
    发明申请
    LIQUID SUPPLY DRIVE MECHANISM USING OSMOTIC PUMP AND MICROCHIP HAVING THE LIQUID SUPPLY DRIVE MECHANISM 有权
    液体供应驱动机构使用OSMOTIC PUMP和具有液体供应驱动机构的MICROCHIP

    公开(公告)号：US20100254832A1

    公开(公告)日：2010-10-07

    申请号：US12742078

    申请日：2008-11-06

    申请人： Takashi Nakazawa

    发明人： Takashi Nakazawa

    IPC分类号： F04B47/08

    CPC分类号： B01L3/50273 ,  B01L2400/046 ,  B01L2400/0472 ,  F04B43/10

    摘要： Provided is a microchip having a liquid supply mechanism which can supply a solution in a micro flow path with a simple construction in which a drive source utilizing an osmotic pressure is arranged within the microchip and which controls the osmotic pressure by a control unit utilizing temperature or the like, whereby it is possible to effect drive control such as intermittent driving and continuous driving at a fixed velocity. The liquid supply mechanism using osmotic pressure includes an osmotic pump utilizing the osmotic pressure of a liquid filling chambers separated from each other by a semipermeable membrane, and a unit which varies the osmotic pressure by changing a condition of the solution in at least one of the chambers on the basis of a timing of a pumping operation.

    摘要翻译： 本发明提供一种具有液体供给机构的微芯片，该液体供给机构能够以简单的结构向微流路供给溶液，在该结构中，使用渗透压的驱动源配置在微芯片内，利用温度控制单元控制渗透压， 从而可以以固定速度实现间歇驱动和连续驱动等驱动控制。 使用渗透压的液体供给机构包括使用通过半透膜隔开的液体填充室的渗透压的渗透泵，以及通过在至少一个中的至少一个中改变溶液的状态来改变渗透压的单元 基于泵送操作的定时。

    Method for selectively collecting N-terminal peptide fragment of protein
    10.
    发明授权
    Method for selectively collecting N-terminal peptide fragment of protein 失效
    选择性收集蛋白质N-末端肽片段的方法

    公开(公告)号：US07041472B2

    公开(公告)日：2006-05-09

    申请号：US10739111

    申请日：2003-12-19

    申请人： Shigemi Norioka ,  Norikazu Ueyama ,  Taka-aki Okamura ,  Takashi Nakazawa ,  Minoru Yamaguchi ,  Eiji Ando

    发明人： Shigemi Norioka ,  Norikazu Ueyama ,  Taka-aki Okamura ,  Takashi Nakazawa ,  Minoru Yamaguchi ,  Eiji Ando

    IPC分类号： C12P21/06

    CPC分类号： C12P21/06

    摘要： The present invention provides a method for selectively collecting the N-terminal peptide fragments of a protein of interest whether or not the protein of interest is modified on the N-terminus. A method for selectively collecting the N-terminal peptide fragment of a protein, comprising: a protection step (1) of protecting side chain-amino groups of amino acid residues containing side chain-amino groups of a protein of interest to obtain a protected protein protected on the side chain-amino groups; a fragmentation step (2) of cleaving the protected protein into one N-terminal peptide fragment (a) containing the N-terminus of the peptide of interest and one or more of peptide fragments (b) other than the N-terminal peptide fragment (a); and a separation step (3) of separating the N-terminal peptide fragment (a) from the other peptide fragments (b) by selectively eluting the N-terminal peptide fragment (a) based on the difference in their reactivity or affinity to substrate, wherein the selective elution is achieved either by allowing the other peptide fragments (b) to bind to the substrate while allowing the N-terminal peptide fragment (a) to elute, or by allowing the N-terminal peptide fragment (a) to bind to the substrate while allowing the other peptide fragments (b) to elute and subsequently eluting the bound N-terminal peptide fragment (a).

    摘要翻译： 本发明提供了一种用于选择性收集目的蛋白质的N-末端肽片段的方法，无论目的蛋白质是否在N末端被修饰。 一种用于选择性收集蛋白质的N-末端肽片段的方法，包括：保护步骤（1）保护含有目的蛋白质的侧链 - 氨基的氨基酸残基的侧链氨基以获得受保护的蛋白质 保护侧链氨基; 将保护的蛋白质切割成包含目标肽的N末端的一个N-末端肽片段（a）和除了N-末端肽片段之外的一个或多个肽片段（b）的片段化步骤（2） 一个）; 以及通过基于它们对底物的反应性或亲和力的差异来选择性地洗脱N末端肽片段（a），从而将N-末端肽片段（a）与其它肽片段（b）分离的分离步骤（3） 其中所述选择性洗脱通过使其它肽片段（b）与底物结合同时允许N-末端肽片段（a）洗脱，或通过使N-末端肽片段（a）与 同时允许其他肽片段（b）洗脱并随后洗脱结合的N-末端肽片段（a）。