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    Methods for nucleic acid amplification with thermostable ribonuclease H
    1.
    发明授权
    Methods for nucleic acid amplification with thermostable ribonuclease H 失效
    用热稳定核糖核酸酶H进行核酸扩增的方法

    公开(公告)号：US5972607A

    公开(公告)日：1999-10-26

    申请号：US893023

    申请日：1997-07-15

    申请人： Motohiro Kondo ,  Toshiya Aono ,  Katsuya Daimon

    发明人： Motohiro Kondo ,  Toshiya Aono ,  Katsuya Daimon

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N9/22 ,  C12Q1/68 ,  C07H21/02 ,  C12N9/00 ,  C12P19/34

    CPC分类号： C12Q1/6865 ,  C12Q1/701

    摘要： Methods for nucleic acid amplification with thermostable ribonuclease H, in which single-stranded RNA (-) is prepared from RNA (+) as a target nucleic acid and the copy number of the single-stranded RNA (-) is increased through the use of thermostable ribonuclease H in combination with non-thermostable RNA-dependent DNA polymerase, non-thermostable DNA-dependent DNA polymerase and non-thermostable DNA-dependent RNA polymerase. In these methods, the number of amplification cycles is well increased and the sensitivity of detection can, therefore, be improved, as compared with the conventional methods. Also provided are methods for the detection of a target nucleic acid from RNA copies of a specific nucleic acid, obtained by any of the amplification methods, and reagent kits for use in these methods.

    摘要翻译： 用作为靶核酸的RNA（+）制备单链RNA（ - ）和单链RNA（ - ）拷贝数的热稳定核糖核酸酶H进行核酸扩增的方法通过使用 热稳定核糖核酸酶H与非热稳定的RNA依赖性DNA聚合酶，非热稳定DNA依赖性DNA聚合酶和非热稳定DNA依赖性RNA聚合酶组合。 在这些方法中，与常规方法相比，扩增循环的数量很好地增加，因此可以提高检测灵敏度。 还提供了用于通过任何扩增方法获得的特异性核酸的RNA拷贝和用于这些方法的试剂盒来检测靶核酸的方法。

    Nucleic acid extraction method
    2.
    发明授权
    Nucleic acid extraction method 失效
    核酸提取法

    公开(公告)号：US06255478B1

    公开(公告)日：2001-07-03

    申请号：US09476379

    申请日：2000-01-03

    申请人： Shigeru Komai ,  Katsuya Daimon ,  Yutaka Takarada

    发明人： Shigeru Komai ,  Katsuya Daimon ,  Yutaka Takarada

    IPC分类号： C07H2100

    CPC分类号： B01L3/502 ,  B01L3/5082 ,  B01L2200/026 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/049 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N15/1013 ,  G01N1/405 ,  G01N35/0098 ,  G01N2035/0436 ,  Y10S435/82

    摘要： This invention provides an apparatus for extracting nucleic acids from nucleic acid-containing samples, particularly biological samples, and more particularly to a nucleic acid extraction apparatus well suited for the nucleic acid extraction method utilizing a nucleic acid-binding magnetic carrier. The nucleic acid extraction apparatus of the invention comprises (1) a group of extraction vessels each comprising a reactor tube in which a sample, a reagent solution, and a magnetic carrier are admixed and reacted, a drain cup for pooling an unwanted component solution, and a nucleic acid recovery tube all as secured to a support, (2) a distribution means for introducing a solution into each of the extraction vessels, (3) a stirring means for mixing the solution and magnetic carrier in the reactor tube, (4) a holding means for holding the magnetic carrier stationary within the vessel, (5) a discharging means for discharging the solution from the reactor tube while the magnetic carrier is held stationary, (6) a heating means for heating the solution and magnetic carrier in the reactor tube, and (7) a transfer means for serially transferring the vessels to the given positions where the distribution means, stirring means, holding means, discharging means and heating means are respectively disposed.

    摘要翻译： 本发明提供了一种用于从含核酸的样品，特别是生物样品中提取核酸的装置，更具体地说，涉及一种利用核酸结合磁性载体的核酸提取方法。 本发明的核酸提取装置包括（1）一组提取容器，每个提取容器均包含反应器管，其中将样品，试剂溶液和磁性载体混合并反应，用于汇集不想要的组分溶液的排水杯， （2）用于将溶液引入每个提取容器的分配装置，（3）用于将溶液和磁性载体混合在反应器管中的搅拌装置，（4） ）用于将磁性载体固定在容器内的保持装置，（5）一个放电装置，用于在磁性载体保持静止时将溶液从反应器管排出;（6）加热装置，用于将溶液和磁性载体加热 反应器管和（7）用于将容器串联地转移到分配装置，搅拌装置，保持装置，排出装置和加热装置相应的给定位置的传送装置 被处置。

    Method for production of recombinant human FSH
    3.
    发明授权
    Method for production of recombinant human FSH 有权
    重组人FSH的生产方法

    公开(公告)号：US07939296B2

    公开(公告)日：2011-05-10

    申请号：US12466652

    申请日：2009-05-15

    申请人： Atsushi Sugimura ,  Katsuya Daimon ,  Kazutoshi Mihara ,  Yae Ito

    发明人： Atsushi Sugimura ,  Katsuya Daimon ,  Kazutoshi Mihara ,  Yae Ito

    IPC分类号： C12N15/09 ,  A23J1/00

    CPC分类号： C07K14/59

    摘要： Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order.

    摘要翻译： 公开了以高产率和高纯度生产重组人FSH的方法。 该方法包括以下步骤：（a）在无血清培养基中培养重组人FSH产生哺乳动物细胞，（b）收集培养上清，（c）使培养上清液进行阳离子交换柱层析，（d）染料 亲和柱层析，（e）疏水柱层析，和（f）凝胶过滤柱层析，依次收集重组人FSH活性级分。

    METHOD FOR PRODUCING AMINOPEPTIDASE
    4.
    发明申请
    METHOD FOR PRODUCING AMINOPEPTIDASE 有权
    生产氨基转移酶的方法

    公开(公告)号：US20110027859A1

    公开(公告)日：2011-02-03

    申请号：US12740146

    申请日：2008-10-25

    申请人： Hiroyuki Sonoda ,  Katsuya Daimon

    发明人： Hiroyuki Sonoda ,  Katsuya Daimon

    IPC分类号： C12N9/48 ,  C07H21/04 ,  C12N15/70 ,  C12N1/21

    CPC分类号： C12N9/48 ,  C12N9/52 ,  C12P13/24 ,  C12P21/06

    摘要： Disclosed is an efficient method for production of aminopeptidase. The method comprises either transforming host bacteria with an aminopeptidase gene and with a neutral protease gene, or transforming some part of host bacteria with an aminopeptidase gene while transforming the other part of the host bacteria with a neutral protease gene, culturing in a medium the hose bacteria transformed with the aminopeptidase gene and with the neutral protease gene, or culturing a mixture of the host bacteria transformed with the aminopeptidase gene and the host bacteria transformed with the neutral protease gene, to let both the aminopeptidase and the neutral protease be expressed, and collecting the aminopeptidase thus produced from the culture mixture.

    摘要翻译： 公开了生产氨基肽酶的有效方法。 该方法包括用氨基肽酶基因和中性蛋白酶基因转化宿主细菌，或用氨基肽酶基因转化一部分宿主细菌，同时用中性蛋白酶基因转化宿主细菌的另一部分，在培养基中培养软管 用氨基肽酶基因和中性蛋白酶基因转化的细菌，或培养用氨基肽酶基因转化的宿主细菌与用中性蛋白酶基因转化的宿主细菌的混合物，使氨基肽酶和中性蛋白酶均表达， 收集由培养混合物制得的氨基肽酶。

    Method for Production of Recombinant Human FSH
    5.
    发明申请
    Method for Production of Recombinant Human FSH 有权
    重组人FSH的生产方法

    公开(公告)号：US20090291473A1

    公开(公告)日：2009-11-26

    申请号：US12466652

    申请日：2009-05-15

    申请人： Atsushi Sugimura ,  Katsuya Daimon ,  Kazutoshi Mihara ,  Yae Ito

    发明人： Atsushi Sugimura ,  Katsuya Daimon ,  Kazutoshi Mihara ,  Yae Ito

    IPC分类号： C12P21/06

    CPC分类号： C07K14/59

    摘要： Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order.

    摘要翻译： 公开了以高产率和高纯度生产重组人FSH的方法。 该方法包括以下步骤：（a）在无血清培养基中培养重组人FSH产生哺乳动物细胞，（b）收集培养上清，（c）使培养上清液进行阳离子交换柱层析，（d）染料 亲和柱层析，（e）疏水柱层析，和（f）凝胶过滤柱层析，依次收集重组人FSH活性级分。

    Method of extracting nucleic acids using particulate carrier
    8.
    发明授权
    Method of extracting nucleic acids using particulate carrier 失效
    使用颗粒载体提取核酸的方法

    公开(公告)号：US06582922B1

    公开(公告)日：2003-06-24

    申请号：US10067208

    申请日：2002-02-07

    申请人： Katsuya Daimon ,  Shigeru Komai ,  Yutaka Takarada

    发明人： Katsuya Daimon ,  Shigeru Komai ,  Yutaka Takarada

    IPC分类号： C12Q168

    CPC分类号： C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q2565/137

    摘要： The present invention provides a method of extracting and isolating nucleic acids from a material containing nucleic acids using a nucleic acid-binding particulate carrier. More specifically, the present invention provides a nucleic acid extraction method using a particulate carrier having a particle diameter of 0.5 to 15.0 &mgr;m, a pore diameter of 50 to 500 nm and a pore volume of 200 to 5000 mm3/g. According to the method of the invention, nucleic acids can be efficiently extracted from a biological material, in particular a material containing a large amount of contaminants, such as a clinical sample.

    摘要翻译： 本发明提供了使用核酸结合颗粒载体从含有核酸的材料中提取和分离核酸的方法。 更具体地说，本发明提供使用粒径为0.5〜15.0μm，孔径为50〜500nm，孔体积为200〜5000mm 3 / g的粒状载体的核酸提取方法。 根据本发明的方法，可以从生物材料，特别是含有大量污染物的材料（例如临床样品）中有效地提取核酸。

    Method for producing aminopeptidase
    9.
    发明授权
    Method for producing aminopeptidase 有权
    氨基肽酶的制备方法

    公开(公告)号：US08685691B2

    公开(公告)日：2014-04-01

    申请号：US12740146

    申请日：2008-10-25

    申请人： Hiroyuki Sonoda ,  Katsuya Daimon ,  Atsushi Sugimura

    发明人： Hiroyuki Sonoda ,  Katsuya Daimon ,  Atsushi Sugimura

    IPC分类号： C12N9/48 ,  C12N1/20 ,  C12N15/00 ,  C12P21/06 ,  C07H21/04

    CPC分类号： C12N9/48 ,  C12N9/52 ,  C12P13/24 ,  C12P21/06

    摘要： Disclosed is an efficient method for production of aminopeptidase. The method comprises either transforming host bacteria with an aminopeptidase gene and with a neutral protease gene, or transforming some part of host bacteria with an aminopeptidase gene while transforming the other part of the host bacteria with a neutral protease gene, culturing in a medium the hose bacteria transformed with the aminopeptidase gene and with the neutral protease gene, or culturing a mixture of the host bacteria transformed with the aminopeptidase gene and the host bacteria transformed with the neutral protease gene, to let both the aminopeptidase and the neutral protease be expressed, and collecting the aminopeptidase thus produced from the culture mixture.

    摘要翻译： 公开了生产氨基肽酶的有效方法。 该方法包括用氨基肽酶基因和中性蛋白酶基因转化宿主细菌，或用氨基肽酶基因转化一部分宿主细菌，同时用中性蛋白酶基因转化宿主细菌的另一部分，在培养基中培养软管 用氨基肽酶基因和中性蛋白酶基因转化的细菌，或培养用氨基肽酶基因转化的宿主细菌与用中性蛋白酶基因转化的宿主细菌的混合物，使氨基肽酶和中性蛋白酶均表达， 收集由培养混合物制得的氨基肽酶。

    Nucleic acid extraction apparatus
    10.
    发明授权
    Nucleic acid extraction apparatus 失效
    核酸提取装置

    公开(公告)号：US06281008B1

    公开(公告)日：2001-08-28

    申请号：US09241242

    申请日：1999-02-01

    申请人： Shigeru Komai ,  Katsuya Daimon ,  Yutaka Takarada

    发明人： Shigeru Komai ,  Katsuya Daimon ,  Yutaka Takarada

    IPC分类号： C12M300

    CPC分类号： B01L3/502 ,  B01L3/5082 ,  B01L2200/026 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/049 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N15/1013 ,  G01N1/405 ,  G01N35/0098 ,  G01N2035/0436 ,  Y10S435/82

    摘要： This invention provides an apparatus for extracting nucleic acids from nucleic acid-containing samples. The nucleic acid extraction apparatus of the invention comprises (1) a group of extraction vessels each comprising a reactor tube in which a sample, a reagent solution, and a magnetic carrier are admixed and reacted, a drain cup for pooling an unwanted component solution, and a nucleic acid recovery tube all as secured to a support, (2) a distribution means for introducing a solution into each of the extraction vessels, (3) a stirring means for mixing the solution and magnetic carrier in the reactor tube, (4) a holding means for holding the magnetic carrier stationary within the vessel, (5) a discharging means for discharging the solution from the reactor tube while the magnetic carrier is held stationary, (6) a heating means for heating the solution and magnetic carrier in the reactor tube, and (7) a transfer means for serially transferring the vessels to the given positions.

    摘要翻译： 本发明提供了一种从含核酸样品中提取核酸的装置。 本发明的核酸提取装置包括（1）一组提取容器，每个提取容器均包含反应器管，其中将样品，试剂溶液和磁性载体混合并反应，用于汇集不需要的组分溶液的排水杯， （2）用于将溶液引入每个提取容器的分配装置，（3）用于将溶液和磁性载体混合在反应器管中的搅拌装置，（4） ）用于将磁性载体固定在容器内的保持装置，（5）一个放电装置，用于在磁性载体保持静止时将溶液从反应器管排出;（6）加热装置，用于将溶液和磁性载体加热 反应器管，和（7）用于将容器串联转移到给定位置的转移装置。