公开(公告)号：US20100227386A1

公开(公告)日：2010-09-09

申请号：US12438725

申请日：2007-08-24

申请人： Pavel Neuzil ,  Juergen Pipper ,  Lukas Novak

发明人： Pavel Neuzil ,  Juergen Pipper ,  Lukas Novak

IPC分类号： C12M1/34 ,  G01J1/58

CPC分类号： G01N21/645 ,  B01L3/5088 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/1827 ,  G01N21/0332 ,  G01N21/6428 ,  G01N21/76 ,  G01N21/763 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/0221 ,  G01N2201/0625 ,  G01N2201/0627 ,  G01N2201/0693

摘要： A detection system is provided, the detection system comprising a light source that generates excitation light having a wavelength sufficient to excite a fluorophore in a sample; an excitation filter positioned along a first line along a path of the excitation light, the excitation filter transmitting the excitation light from the light source; a beam splitter positioned along the first line, the beam splitter reflecting the excitation light transmitted by the excitation filter along a second line toward a mirror positioned on one side of the beam splitter, and passing emitted light reflected along the second line; the mirror, positioned to reflect the excitation light from the beam splitter to the fluorophore in the sample along a third line, normal to both the first and second lines, wherein the mirror further reflects emitted light emitted along the third line, along the second line toward the beam splitter; an emission filter positioned along the second line, on a second side of the beam splitter; and a detector that detects the emitted light transmitted by the emission filter.

摘要翻译： 提供一种检测系统，所述检测系统包括产生具有足以激发样品中的荧光团的波长的激发光的光源; 激励滤光器，其沿着激励光的路径沿着第一线定位，所述激发滤光器透射来自所述光源的激发光; 分束器，沿着第一线定位，分束器将由激励滤光器透射的激发光沿着第二线反射到位于分束器一侧的反射镜，并且使沿着第二线反射的发射光反射; 所述反射镜被定位成将来自分束器的激发光沿着与第一和第二线垂直的第三线反射到样品中的荧光团，其中反射镜还沿着第二条线反射沿着第三条线发射的发射光 朝向分束器; 沿着所述第二线定位在所述分束器的第二侧上的发射滤光器; 以及检测由发射滤波器发射的发射光的检测器。

公开(公告)号：US20120244605A1

公开(公告)日：2012-09-27

申请号：US13346418

申请日：2012-01-09

申请人： Pavel Neuzil ,  Tseng-Ming Hsieh ,  Juergen Pipper

发明人： Pavel Neuzil ,  Tseng-Ming Hsieh ,  Juergen Pipper

IPC分类号： C12M1/38 ,  G01N1/28

CPC分类号： B01L3/5088 ,  B01J2219/00495 ,  B01L3/5027 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/1822 ,  B01L2300/1827 ,  G01N21/0332 ,  G01N25/18

摘要： The invention provides an apparatus for regulating the temperature of a chemical and/or biological sample and a method of using the same. The apparatus includes at least one temperature control module. The temperature control module includes a heater, a conductor of caloric, and a temperature sensor. The heater of the temperature control module is adapted to thermally communicate with a removable substrate, on which said chemical and/or biological sample is placed, via the conductor of caloric. The temperature sensor of the temperature control module is adapted to detect and control the temperature of the substrate via the conductor of caloric. The apparatus is designed such that the substrate is situated above said temperature control module to entirely cover said temperature control module.

摘要翻译： 本发明提供了一种用于调节化学和/或生物样品的温度的装置及其使用方法。 该装置包括至少一个温度控制模块。 温度控制模块包括加热器，热量导体和温度传感器。 温度控制模块的加热器适于通过热量导体与其上放置所述化学和/或生物样品的可移除基板热连通。 温度控制模块的温度传感器适于通过热量导体检测和控制基板的温度。 设备被设计成使得基板位于所述温度控制模块上方以完全覆盖所述温度控制模块。

公开(公告)号：US20090289213A1

公开(公告)日：2009-11-26

申请号：US12279308

申请日：2006-02-13

申请人： Juergen Pipper ,  Tseng-Ming Hsieh ,  Pavel Neuzil

发明人： Juergen Pipper ,  Tseng-Ming Hsieh ,  Pavel Neuzil

IPC分类号： H01F1/00

CPC分类号： C07K1/22 ,  B01D15/3804 ,  B01J20/28009 ,  B01J2220/54 ,  B03C1/01 ,  C12M25/01 ,  G01N33/5432 ,  G01N33/5436 ,  G01N2446/00

摘要： The invention provides a method of processing a biological and/or chemical sample. The method includes providing a fluid droplet, which includes an inner phase and an outer phase. The outer phase is immiscible with the inner phase, and the outer phase is surrounding the inner phase. The inner phase includes the biological and/or chemical sample. The fluid droplet furthermore comprises magnetically attractable matter. The method also includes providing at least one surface, which is of such a texture and such a wettability for the fluid of the inner phase of the fluid droplet, that the fluid droplet remains intact upon being contacted therewith. The method further includes disposing the fluid droplet onto the at least one surface. The method also includes performing a process on the biological and/or chemical sample in the fluid droplet.

摘要翻译： 本发明提供了一种处理生物和/或化学样品的方法。 该方法包括提供包括内相和外相的流体液滴。 外相与内相不混溶，外相围绕内相。 内相包括生物和/或化学样品。 流体液滴还包括磁吸引物质。 该方法还包括提供至少一个表面，其具有这样的质地和对流体液滴的内相的流体的润湿性，使得流体液滴在与其接触时保持完整。 该方法还包括将流体液滴设置在至少一个表面上。 该方法还包括对流体液滴中的生物和/或化学样品进行处理。

公开(公告)号：US08124033B2

公开(公告)日：2012-02-28

申请号：US11356511

申请日：2006-02-17

申请人： Pavel Neuzil ,  Tseng-Ming Hsieh ,  Juergen Pipper

发明人： Pavel Neuzil ,  Tseng-Ming Hsieh ,  Juergen Pipper

IPC分类号： B01L9/00

CPC分类号： B01L3/5088 ,  B01J2219/00495 ,  B01L3/5027 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/1822 ,  B01L2300/1827 ,  G01N21/0332 ,  G01N25/18

摘要： The invention provides an apparatus for regulating the temperature of a chemical and/or biological sample and a method of using the same. The apparatus includes at least one temperature control module. The temperature control module includes a heater, a conductor of caloric, and a temperature sensor. The heater of the temperature control module is adapted to thermally communicate with a removable substrate, on which said chemical and/or biological sample is placed, via the conductor of caloric. The temperature sensor of the temperature control module is adapted to detect and control the temperature of the substrate via the conductor of caloric. The apparatus is designed such that the substrate is situated above said temperature control module to entirely cover said temperature control module.

摘要翻译： 本发明提供了一种用于调节化学和/或生物样品的温度的装置及其使用方法。 该装置包括至少一个温度控制模块。 温度控制模块包括加热器，热量导体和温度传感器。 温度控制模块的加热器适于通过热量导体与其上放置所述化学和/或生物样品的可移除基板热连通。 温度控制模块的温度传感器适于通过热量导体检测和控制基板的温度。 设备被设计成使得基板位于所述温度控制模块上方以完全覆盖所述温度控制模块。

公开(公告)号：US20070196237A1

公开(公告)日：2007-08-23

申请号：US11356511

申请日：2006-02-17

申请人： Pavel Neuzil ,  Tseng-Ming Hsieh ,  Juergen Pipper

发明人： Pavel Neuzil ,  Tseng-Ming Hsieh ,  Juergen Pipper

IPC分类号： G01N31/00

CPC分类号： B01L3/5088 ,  B01J2219/00495 ,  B01L3/5027 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/1822 ,  B01L2300/1827 ,  G01N21/0332 ,  G01N25/18

摘要： The invention provides an apparatus for regulating the temperature of a chemical and/or biological sample and a method of using the same. The apparatus includes at least one temperature control module. The temperature control module includes a heater, a conductor of caloric, and a temperature sensor. The heater of the temperature control module is adapted to thermally communicate with a removable substrate, on which said chemical and/or biological sample is placed, via the conductor of caloric. The temperature sensor of the temperature control module is adapted to detect and control the temperature of the substrate via the conductor of caloric. The apparatus is designed such that the substrate is situated above said temperature control module to entirely cover said temperature control module.

摘要翻译： 本发明提供了一种用于调节化学和/或生物样品的温度的装置及其使用方法。 该装置包括至少一个温度控制模块。 温度控制模块包括加热器，热量导体和温度传感器。 温度控制模块的加热器适于通过热量导体与其上放置所述化学和/或生物样品的可移除基板热连通。 温度控制模块的温度传感器适于通过热量导体检测和控制基板的温度。 设备被设计成使得基板位于所述温度控制模块上方以完全覆盖所述温度控制模块。

公开(公告)号：US08216855B2

公开(公告)日：2012-07-10

申请号：US12279308

申请日：2006-02-13

申请人： Juergen Pipper ,  Tseng-Ming Hsieh ,  Pavel Neuzil

发明人： Juergen Pipper ,  Tseng-Ming Hsieh ,  Pavel Neuzil

IPC分类号： G01N33/553

CPC分类号： C07K1/22 ,  B01D15/3804 ,  B01J20/28009 ,  B01J2220/54 ,  B03C1/01 ,  C12M25/01 ,  G01N33/5432 ,  G01N33/5436 ,  G01N2446/00

摘要： The invention provides a method of processing a biological and/or chemical sample. The method includes providing a fluid droplet, which includes an inner phase and an outer phase. The outer phase is immiscible with the inner phase, and the outer phase is surrounding the inner phase. The inner phase includes the biological and/or chemical sample. The fluid droplet furthermore comprises magnetically attractable matter. The method also includes providing at least one surface, which is of such a texture and such a wettability for the fluid of the inner phase of the fluid droplet, that the fluid droplet remains intact upon being contacted therewith. The method further includes disposing the fluid droplet onto the at least one surface. The method also includes performing a process on the biological and/or chemical sample in the fluid droplet.

摘要翻译： 本发明提供了一种处理生物和/或化学样品的方法。 该方法包括提供包括内相和外相的流体液滴。 外相与内相不混溶，外相围绕内相。 内相包括生物和/或化学样品。 流体液滴还包括磁吸引物质。 该方法还包括提供至少一个表面，其具有这样的质地和对流体液滴的内相的流体的润湿性，使得流体液滴在与其接触时保持完整。 该方法还包括将流体液滴设置在至少一个表面上。 该方法还包括对流体液滴中的生物和/或化学样品进行处理。

公开(公告)号：US20090263870A1

公开(公告)日：2009-10-22

申请号：US12428901

申请日：2009-04-23

申请人： Juergen Pipper ,  Pavel Neuzil ,  Yi Zhang ,  Muhammad Khidhir Khairudin ,  Tseng-Ming Hsieh

发明人： Juergen Pipper ,  Pavel Neuzil ,  Yi Zhang ,  Muhammad Khidhir Khairudin ,  Tseng-Ming Hsieh

IPC分类号： C12P19/34 ,  C12M1/00

CPC分类号： C12P19/34 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2563/137

摘要： There is provided a method and/or system which allow on-chip preconditioning of complex real-world samples and/or handling of limited amounts of target material, and/or on-chip nucleic acid amplification process, using a free droplet containing magnetic attractable material. The nucleic acid amplification process comprises controlling the position of the magnetic attractable material and performing the nucleic acid amplification in a thermocycling droplet located onto at least one temperature zone. The low thermal masses of the herein described heaters/temperature sensors come along with fast temperature transitions within the corresponding temperature zones allowing impressing temperature gradients in at least one temperature zone between subsequent or within the same thermocycle(s). Additionally, the variable residence times of the droplet in a given temperature zone permit to customize the denaturation, annealing and/or extension times within the same or between different PCR runs. Additionally, the herein described method and/or system allow amplification of one or more nucleic acid molecules. Additionally, the herein described method and/or system allow real-time monitoring with or without the presence of magnetic attractable material bound to said nucleic acid molecule.

摘要翻译： 提供了一种方法和/或系统，其允许使用包含磁性可吸引的自由液滴来对复杂的现实世界样品进行片上预处理和/或处理有限量的靶材料和/或片上核酸扩增过程 材料。 核酸扩增方法包括控制磁性可吸引材料的位置并在位于至少一个温度区域上的热循环液滴中进行核酸扩增。 本文描述的加热器/温度传感器的低热质量伴随着相应温度区域内的快速温度转变，允许在相同或相同的热循环之间的至少一个温度区域内施加温度梯度。 此外，液滴在给定温度区域的可变停留时间允许在相同或不同PCR运行之间定制变性，退火和/或延伸时间。 另外，本文所述的方法和/或系统允许扩增一个或多个核酸分子。 另外，本文描述的方法和/或系统允许使用或不存在与所述核酸分子结合的可磁性吸引材料的实时监测。

公开(公告)号：US07439073B2

公开(公告)日：2008-10-21

申请号：US10372288

申请日：2003-02-25

申请人： Toru Fujimura ,  Juergen Pipper

发明人： Toru Fujimura ,  Juergen Pipper

IPC分类号： G01N21/00 ,  G01N33/543 ,  G01N1/10 ,  C12Q1/70 ,  G01B11/02

CPC分类号： G01N21/55 ,  G01N21/45

摘要： An object of the present invention is to provide a simple biochemical sensor making use of the light interference effect of an optical film which is capable of measuring binding of biochemical substances at a high throughput. In the present invention, a solution containing samples 18 is passed through the sensor having probes 17 provided on an optical film with a refractive index of not less than 1.8 and not greater than 3 on a substrate, and binding of the probes 17 and samples 18 is detected from the change of intensity of the reflected light. This makes it possible to measure binding of biochemical substances at a high throughput with high precision.

摘要翻译： 本发明的目的是提供一种利用光学膜的光干涉效应的简单的生物化学传感器，其能够以高生产量测量生化物质的结合。 在本发明中，将含有样品18的溶液在衬底上通过具有折射率不小于1.8且不大于3的光学膜上提供的探针17的传感器，并且探针17和样品18的结合 从反射光的强度的变化来检测。 这使得可以高精度地测量生物化学物质在高产量下的结合。

公开(公告)号：US20120064534A1

公开(公告)日：2012-03-15

申请号：US13255894

申请日：2010-03-10

申请人： Juergen Pipper ,  Jiri Husak ,  Marek Martinkovic

发明人： Juergen Pipper ,  Jiri Husak ,  Marek Martinkovic

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B03C3/017 ,  B03C2201/18 ,  B03C2201/26 ,  G01N21/0332 ,  G01N21/65 ,  G01N35/0098 ,  G01N2021/035 ,  G01N2021/6439 ,  G01N2021/653

摘要： The present invention is directed to an apparatus for processing at least one biological and/or chemical sample. The apparatus comprises a substrate, temperature control modules and a rotatable platform carrying a magnetic field generator and an optical unit. The present invention is further directed to a system including the apparatus and magnetically attractable matter as well as method which can be carried out using the apparatus of the present invention.

摘要翻译： 本发明涉及用于处理至少一种生物和/或化学样品的装置。 该装置包括基板，温度控制模块和承载磁场发生器和光学单元的可旋转平台。 本发明还涉及一种包括该装置和磁吸引物质的系统以及可以使用本发明的装置进行的方法。