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公开(公告)号：US5958342A
公开(公告)日：1999-09-28
申请号：US649535
申请日：1996-05-17
CPC分类号： B01L3/0268 , B01J19/0046 , B82Y30/00 , B01J2219/00378 , B01J2219/00531 , B01J2219/00533 , B01J2219/00536 , B01J2219/00605 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00691 , B01L2200/04 , C40B50/14 , C40B60/14 , G01N2001/282 , G01N2035/1041
摘要： Devices and methods are provided for precise production of arrays of microspots. A pulse jetting device is employed having a capillary of micron dimensions, with a portion of the capillary proximal to the jetting orifice circumferentially surrounded by a piezoelectric transducer. By appropriate design of the capillary, orifice and piezoelectric transducer, droplets can be formed on a surface, separated by as little as 80 microns center-to-center, and having at least about a 15 micron spacing at the border. The subject substrate arrays can be used for providing miniaturized arrays of reagents, such as nucleic acids, for detecting the presence of homologous sequences in a sample.
摘要翻译： 提供了用于精确生产微孢子体阵列的装置和方法。 使用脉冲喷射装置具有微米尺寸的毛细管，毛细管的一部分靠近喷射孔，周向地被压电换能器包围。 通过毛细管，孔口和压电换能器的适当设计，液滴可以形成在中心至中心微小至80微米的表面上，并且在边界处具有至少约15微米的间距。 主题衬底阵列可用于提供用于检测样品中同源序列的存在的小型试剂阵列，例如核酸。
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公开(公告)号：US6001309A
公开(公告)日：1999-12-14
申请号：US79871
申请日：1998-05-15
CPC分类号： B01L3/0268 , B01J19/0046 , B82Y30/00 , B01J2219/00378 , B01J2219/00531 , B01J2219/00533 , B01J2219/00536 , B01J2219/00605 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00691 , B01L2200/04 , C40B50/14 , C40B60/14 , G01N2001/282 , G01N2035/1041
摘要： Devices and methods are provided for precise reduction of arrays of microspots. A pulse jetting device is employed having a capillary of micron dimensions, with a portion of the capillary proximal to the jetting orifice circumferentially surrounded by a piezoelectric transducer. By appropriate design of the capillary, orifice and piezoelectric transducer, droplets can be formed on a surface, separated by as little as 80 microns center-to-center, and having at least about a 15 micron spacing at the border. The subject substrate arrays can be used for providing miniaturized arrays of reagents, such as nucleic acids, for detecting the presence of homologous sequences in a sample.
摘要翻译： 提供了设备和方法来精确地减少微孢子体阵列。 使用脉冲喷射装置具有微米尺寸的毛细管，毛细管的一部分靠近喷射孔，周向地被压电换能器包围。 通过毛细管，孔口和压电换能器的适当设计，液滴可以形成在中心至中心微小至80微米的表面上，并且在边界处具有至少约15微米的间距。 主题衬底阵列可用于提供用于检测样品中同源序列的存在的小型试剂阵列，例如核酸。
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3.发明授权
公开(公告)号：US6015880A
公开(公告)日：2000-01-18
申请号：US213857
申请日：1994-03-16
IPC分类号： C12Q1/68 , B01J19/00 , C07H21/00 , C07K1/04 , C40B40/06 , C40B40/10 , C40B50/14 , C40B60/14 , G01N33/53 , G01N37/00 , A61K38/00
CPC分类号： B82Y30/00 , B01J19/0046 , C07H21/00 , C07K1/042 , C07K1/047 , B01J2219/00369 , B01J2219/00378 , B01J2219/00475 , B01J2219/00513 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/0068 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B50/14 , C40B60/14
摘要： A method and apparatus are provided for preparing a substrate upon which is located microdrop-sized loci at which chemical compounds are synthesized or diagnostic tests are conducted. The loci are formed by applying microdrops from a dispenser from which a microdrop is pulse fed onto the surface of the substrate.
摘要翻译： 提供了一种用于制备基底的方法和装置，其上定位有微小尺寸的位置，在该位置处合成化合物或进行诊断测试。 通过将微滴从脉冲馈送到基底表面上的分配器施加微滴而形成位点。
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公开(公告)号：US5847105A
公开(公告)日：1998-12-08
申请号：US910727
申请日：1997-08-13
IPC分类号： C12Q1/68 , B01J19/00 , C07H21/00 , C07K1/04 , C40B40/06 , C40B40/10 , C40B50/14 , C40B60/14 , G01N33/53 , G01N37/00
CPC分类号： B82Y30/00 , B01J19/0046 , C07H21/00 , C07K1/042 , C07K1/047 , B01J2219/00369 , B01J2219/00378 , B01J2219/00475 , B01J2219/00513 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/0068 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B50/14 , C40B60/14
摘要： A method and apparatus are provided for preparing a substrate upon which is located microdrop-sized loci at which chemical compounds are synthesized or diagnostic tests are conducted. The loci are formed by applying microdrops from a dispenser from which a microdrop is pulse fed onto the surface of the substrate.
摘要翻译： 提供了一种用于制备基底的方法和装置，其上定位有微小尺寸的位置，在该位置处合成化合物或进行诊断测试。 通过将微滴从脉冲馈送到基底表面上的分配器施加微滴而形成位点。
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公开(公告)号：US5981733A
公开(公告)日：1999-11-09
申请号：US714867
申请日：1996-09-16
CPC分类号： B01J19/0046 , B01J2219/00378 , B01J2219/00391 , B01J2219/00475 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01J2219/00722 , C40B40/06 , C40B60/14 , Y02P20/582
摘要： An apparatus for the automated synthesis of molecular arrays. A jetting device is employed along with a reaction chamber to dispense reagents used in the synthesis onto the substrate. A positioning system moves the substrate from the jet to the reaction chamber. A controller controls the movement of the substrate and the application of the reagents so that the synthesis is carried out according to a pre-determined procedure. The apparatus will synthesize nucleotides in an array of micron-size spots according to a pattern selected by the operator immediately prior to synthesis.
摘要翻译： 用于自动合成分子阵列的装置。 喷射装置与反应室一起使用以将合成中使用的试剂分配到基材上。 定位系统将基板从射流移动到反应室。 控制器控制衬底的移动和试剂的应用，使得根据预定程序进行合成。 该装置将根据在合成前立即由操作者选择的图案在微米级斑点的阵列中合成核苷酸。
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6.发明授权Methods and solvent vehicles for reagent delivery in oligonucleotide synthesis using automated pulse jetting devices 失效使用自动脉冲喷射装置的寡核苷酸合成中的试剂递送的方法和溶剂载体
公开(公告)号：US5874554A
公开(公告)日：1999-02-23
申请号：US767233
申请日：1996-12-13
CPC分类号： C07H21/00 , B01J19/0046 , B01J2219/00378 , B01J2219/00527 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00659 , B01J2219/00722 , C40B40/06 , C40B60/14
摘要： Solvent vehicles and methods of their use in the pulse jet delivery of reagents in automated oligonucleotide synthesis by the solid-phase phosphoramidite method are provided. For pulse jet delivery of the activated monomer, acetonitrile in combination with at least one stabilizer co-solvent is employed as the solvent vehicle, where the stabilizer co-solvent is an inert, organic solvent having a boiling point of at least 100.degree. C. For pulse jet delivery of the detritylation reagent, a mono- or disubstituted methane is employed as the solvent vehicle, where substituents have a molecular weight greater than 36 daltons. Use of the solvent vehicles according to the subject invention provides for reduced reagent precipitation in the pulse jet tubes and improved results.
摘要翻译： 提供溶剂车辆及其在通过固相亚磷酰胺方法的自动寡核苷酸合成中脉冲喷射输送试剂中的方法。 对于活化单体的脉冲喷射输送，使用乙腈与至少一种稳定剂助溶剂组合作为溶剂载体，其中稳定剂助溶剂是沸点至少为100℃的惰性有机溶剂。 对于去离子试剂的脉冲喷射输送，使用单取代或二取代的甲烷作为溶剂载体，其中取代基的分子量大于36道尔顿。 根据本发明的溶剂载体的使用提供了脉冲喷射管中减少的试剂沉淀和改进的结果。
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公开(公告)号：US20070134248A1
公开(公告)日：2007-06-14
申请号：US11297167
申请日：2005-12-08
申请人： Dan Denney , Keri Marie Tate , Thomas P. Theriault
发明人： Dan Denney , Keri Marie Tate , Thomas P. Theriault
IPC分类号： A61K39/395 , A61K39/00
CPC分类号： C07K16/4283 , C07K16/3061
摘要： The present invention provides combination immunotherapy for Non-Hodgkin's Lymphoma. In one embodiment, the combination immunotherapy first provides for the administration of a monoclonal antibody directed to a non-idotypic portion of a lymphoma cell surface immunoglobulin (e.g. a framework region of a variable region). The combination immunotherapy next provides for the administration of an immunogenic composition comprising at least a portion of the same lymphoma cell surface immunoglobulin, whether an idiotypic portion or non-idiotypic portion.
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公开(公告)号：US06448013B1
公开(公告)日：2002-09-10
申请号：US09721801
申请日：2000-11-22
申请人： Lyle Arnold , Tod Bedilion , Erik Bieldanes , Thomas P. Theriault , Warren Lei
发明人： Lyle Arnold , Tod Bedilion , Erik Bieldanes , Thomas P. Theriault , Warren Lei
IPC分类号： C12Q168
CPC分类号： C12Q1/6837 , C12Q1/6851 , Y10T436/143333 , C12Q2545/114 , C12Q2531/113 , C12Q2565/501
摘要： Methods are provide for quantitatively determining the amount of polynucleotides in a sample. In one method, the distinct polynucleotide targets and standard polynucleotide targets are contacted with detectable probes and independently detectable single or double stranded complements to the standard targets under hybridization conditions. The hybridization pattern from the probe is compared to the hybridization pattern from the standard sequences to obtain quantitative information about the genetic profile of the labeled nucleic acid sample. Also provided are kits for the use of the methods.
摘要翻译： 提供了定量测定样品中多核苷酸的量的方法。 在一种方法中，在杂交条件下，将不同的多核苷酸靶标和标准多核苷酸靶标与可检测的探针和标准靶的独立可检测的单链或双链互补物接触。 将来自探针的杂交模式与来自标准序列的杂交模式进行比较，以获得关于标记的核酸样品的遗传图谱的定量信息。 还提供了使用方法的工具包。
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公开(公告)号：US06423535B1
公开(公告)日：2002-07-23
申请号：US09449059
申请日：1999-11-24
申请人： Lyle Arnold , Tod Bedilion , Erik Bjeldanes , Thomas P. Theriault , Warren Lei
发明人： Lyle Arnold , Tod Bedilion , Erik Bjeldanes , Thomas P. Theriault , Warren Lei
IPC分类号： C12M134
CPC分类号： C12Q1/6837 , C12Q1/6851 , Y10T436/143333 , C12Q2545/114 , C12Q2531/113 , C12Q2565/501
摘要： Methods are provide for quantitatively determining the amount of polynucleotides in a sample. In one method, the distinct polynucleotide targets and standard polynucleotide targets are contacted with detectable probes and independently detectable single or double stranded complements to the standard targets under hybridization conditions. The hybridization pattern from the probe is compared to the hybridization pattern from the standard sequences to obtain quantitative information about the genetic profile of the labeled nucleic acid sample. Also provided are kits for the use of the methods.
摘要翻译： 提供了定量测定样品中多核苷酸的量的方法。 在一种方法中，在杂交条件下，将不同的多核苷酸靶标和标准多核苷酸靶标与可检测的探针和标准靶标的独立可检测的单链或双链互补物接触。 将来自探针的杂交模式与来自标准序列的杂交模式进行比较，以获得关于标记的核酸样品的遗传图谱的定量信息。 还提供了使用方法的工具包。
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公开(公告)号：US20190342246A1
公开(公告)日：2019-11-07
申请号：US16403607
申请日：2019-05-05
申请人： Thomas P. Theriault , Mark Dingman
发明人： Thomas P. Theriault , Mark Dingman
摘要： The present invention is directed to novel tools and systems that provide methods to monitor and police platforms for interfacing designed to facilitate the exchange of communications between two or more users. In particular, the present invention provides anonymous and secure methods to capture, monitor, and score users of an interfacing platform, such as a dating platform, based on inappropriate and indecent behavior on the interfacing platform.
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