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1.发明授权Methods and solvent vehicles for reagent delivery in oligonucleotide synthesis using automated pulse jetting devices 失效使用自动脉冲喷射装置的寡核苷酸合成中的试剂递送的方法和溶剂载体
公开(公告)号：US5874554A
公开(公告)日：1999-02-23
申请号：US767233
申请日：1996-12-13
CPC分类号： C07H21/00 , B01J19/0046 , B01J2219/00378 , B01J2219/00527 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00659 , B01J2219/00722 , C40B40/06 , C40B60/14
摘要： Solvent vehicles and methods of their use in the pulse jet delivery of reagents in automated oligonucleotide synthesis by the solid-phase phosphoramidite method are provided. For pulse jet delivery of the activated monomer, acetonitrile in combination with at least one stabilizer co-solvent is employed as the solvent vehicle, where the stabilizer co-solvent is an inert, organic solvent having a boiling point of at least 100.degree. C. For pulse jet delivery of the detritylation reagent, a mono- or disubstituted methane is employed as the solvent vehicle, where substituents have a molecular weight greater than 36 daltons. Use of the solvent vehicles according to the subject invention provides for reduced reagent precipitation in the pulse jet tubes and improved results.
摘要翻译： 提供溶剂车辆及其在通过固相亚磷酰胺方法的自动寡核苷酸合成中脉冲喷射输送试剂中的方法。 对于活化单体的脉冲喷射输送，使用乙腈与至少一种稳定剂助溶剂组合作为溶剂载体，其中稳定剂助溶剂是沸点至少为100℃的惰性有机溶剂。 对于去离子试剂的脉冲喷射输送，使用单取代或二取代的甲烷作为溶剂载体，其中取代基的分子量大于36道尔顿。 根据本发明的溶剂载体的使用提供了脉冲喷射管中减少的试剂沉淀和改进的结果。
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2.发明授权
公开(公告)号：US6015880A
公开(公告)日：2000-01-18
申请号：US213857
申请日：1994-03-16
IPC分类号： C12Q1/68 , B01J19/00 , C07H21/00 , C07K1/04 , C40B40/06 , C40B40/10 , C40B50/14 , C40B60/14 , G01N33/53 , G01N37/00 , A61K38/00
CPC分类号： B82Y30/00 , B01J19/0046 , C07H21/00 , C07K1/042 , C07K1/047 , B01J2219/00369 , B01J2219/00378 , B01J2219/00475 , B01J2219/00513 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/0068 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B50/14 , C40B60/14
摘要： A method and apparatus are provided for preparing a substrate upon which is located microdrop-sized loci at which chemical compounds are synthesized or diagnostic tests are conducted. The loci are formed by applying microdrops from a dispenser from which a microdrop is pulse fed onto the surface of the substrate.
摘要翻译： 提供了一种用于制备基底的方法和装置，其上定位有微小尺寸的位置，在该位置处合成化合物或进行诊断测试。 通过将微滴从脉冲馈送到基底表面上的分配器施加微滴而形成位点。
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公开(公告)号：US5958342A
公开(公告)日：1999-09-28
申请号：US649535
申请日：1996-05-17
CPC分类号： B01L3/0268 , B01J19/0046 , B82Y30/00 , B01J2219/00378 , B01J2219/00531 , B01J2219/00533 , B01J2219/00536 , B01J2219/00605 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00691 , B01L2200/04 , C40B50/14 , C40B60/14 , G01N2001/282 , G01N2035/1041
摘要： Devices and methods are provided for precise production of arrays of microspots. A pulse jetting device is employed having a capillary of micron dimensions, with a portion of the capillary proximal to the jetting orifice circumferentially surrounded by a piezoelectric transducer. By appropriate design of the capillary, orifice and piezoelectric transducer, droplets can be formed on a surface, separated by as little as 80 microns center-to-center, and having at least about a 15 micron spacing at the border. The subject substrate arrays can be used for providing miniaturized arrays of reagents, such as nucleic acids, for detecting the presence of homologous sequences in a sample.
摘要翻译： 提供了用于精确生产微孢子体阵列的装置和方法。 使用脉冲喷射装置具有微米尺寸的毛细管，毛细管的一部分靠近喷射孔，周向地被压电换能器包围。 通过毛细管，孔口和压电换能器的适当设计，液滴可以形成在中心至中心微小至80微米的表面上，并且在边界处具有至少约15微米的间距。 主题衬底阵列可用于提供用于检测样品中同源序列的存在的小型试剂阵列，例如核酸。
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公开(公告)号：US5847105A
公开(公告)日：1998-12-08
申请号：US910727
申请日：1997-08-13
IPC分类号： C12Q1/68 , B01J19/00 , C07H21/00 , C07K1/04 , C40B40/06 , C40B40/10 , C40B50/14 , C40B60/14 , G01N33/53 , G01N37/00
CPC分类号： B82Y30/00 , B01J19/0046 , C07H21/00 , C07K1/042 , C07K1/047 , B01J2219/00369 , B01J2219/00378 , B01J2219/00475 , B01J2219/00513 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/0068 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B50/14 , C40B60/14
摘要： A method and apparatus are provided for preparing a substrate upon which is located microdrop-sized loci at which chemical compounds are synthesized or diagnostic tests are conducted. The loci are formed by applying microdrops from a dispenser from which a microdrop is pulse fed onto the surface of the substrate.
摘要翻译： 提供了一种用于制备基底的方法和装置，其上定位有微小尺寸的位置，在该位置处合成化合物或进行诊断测试。 通过将微滴从脉冲馈送到基底表面上的分配器施加微滴而形成位点。
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公开(公告)号：US6001309A
公开(公告)日：1999-12-14
申请号：US79871
申请日：1998-05-15
CPC分类号： B01L3/0268 , B01J19/0046 , B82Y30/00 , B01J2219/00378 , B01J2219/00531 , B01J2219/00533 , B01J2219/00536 , B01J2219/00605 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00691 , B01L2200/04 , C40B50/14 , C40B60/14 , G01N2001/282 , G01N2035/1041
摘要： Devices and methods are provided for precise reduction of arrays of microspots. A pulse jetting device is employed having a capillary of micron dimensions, with a portion of the capillary proximal to the jetting orifice circumferentially surrounded by a piezoelectric transducer. By appropriate design of the capillary, orifice and piezoelectric transducer, droplets can be formed on a surface, separated by as little as 80 microns center-to-center, and having at least about a 15 micron spacing at the border. The subject substrate arrays can be used for providing miniaturized arrays of reagents, such as nucleic acids, for detecting the presence of homologous sequences in a sample.
摘要翻译： 提供了设备和方法来精确地减少微孢子体阵列。 使用脉冲喷射装置具有微米尺寸的毛细管，毛细管的一部分靠近喷射孔，周向地被压电换能器包围。 通过毛细管，孔口和压电换能器的适当设计，液滴可以形成在中心至中心微小至80微米的表面上，并且在边界处具有至少约15微米的间距。 主题衬底阵列可用于提供用于检测样品中同源序列的存在的小型试剂阵列，例如核酸。
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公开(公告)号：US5981733A
公开(公告)日：1999-11-09
申请号：US714867
申请日：1996-09-16
CPC分类号： B01J19/0046 , B01J2219/00378 , B01J2219/00391 , B01J2219/00475 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01J2219/00722 , C40B40/06 , C40B60/14 , Y02P20/582
摘要： An apparatus for the automated synthesis of molecular arrays. A jetting device is employed along with a reaction chamber to dispense reagents used in the synthesis onto the substrate. A positioning system moves the substrate from the jet to the reaction chamber. A controller controls the movement of the substrate and the application of the reagents so that the synthesis is carried out according to a pre-determined procedure. The apparatus will synthesize nucleotides in an array of micron-size spots according to a pattern selected by the operator immediately prior to synthesis.
摘要翻译： 用于自动合成分子阵列的装置。 喷射装置与反应室一起使用以将合成中使用的试剂分配到基材上。 定位系统将基板从射流移动到反应室。 控制器控制衬底的移动和试剂的应用，使得根据预定程序进行合成。 该装置将根据在合成前立即由操作者选择的图案在微米级斑点的阵列中合成核苷酸。
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公开(公告)号：US20110178610A1
公开(公告)日：2011-07-21
申请号：US13120920
申请日：2009-10-15
CPC分类号： G06Q10/06
摘要： A system and method for monitoring and managing utility consumption includes use of (A) one or more sensors arranged to monitor an operating condition and/or utility consumption of at least one segment or appliance of a first facility; (B) a memory including at least one of (i) stored utility consumption information correlated or normalized to at least one designated parameter affecting utility consumption or (ii) hypothetical utility consumption; (C) a processor to compare the monitored information with the stored information, and (D) an output medium to store and/or display the comparison information. Stored consumption information may relate to the first facility or a second facility. Hypothetical utility consumption information may relate to a segment or appliance of the first facility operated according to optimized conditions, or an upgraded segment or appliance.
摘要翻译： 一种用于监测和管理公用事业消耗的系统和方法包括使用（A）布置成监测第一设施的至少一个段或设备的运行状况和/或公用事业消耗的一个或多个传感器; （B）存储器，其包括以下至少一个：（i）存储的与至少一个影响公用事业消耗的指定参数相关或归一化的公用消费信息;（ii）假设的公用事业消费; （C）处理器，用于将所监视的信息与存储的信息进行比较，以及（D）存储和/或显示比较信息的输出介质。 存储的消费信息可能与第一设施或第二设施有关。 假设效用消耗信息可以涉及根据优化条件操作的第一设施的段或设备，或升级的段或设备。
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公开(公告)号：US4497791A
公开(公告)日：1985-02-05
申请号：US465502
申请日：1983-02-10
CPC分类号： A61K9/1272 , A61K51/1203 , A61K51/1227 , A61K51/1234 , A61K2123/00
摘要： Described herein is a process for labeling leukocytes and other phagocytic cells with labeled micellular particles involving incubating the cells with the micellular particles. Also described is a process for detecting the locus of an infection by administering to a subject leukocytes radiolabeled by incubation with labeled micellular particles followed by scanning the subject to detect the locus of radiation emitted by the particles.
摘要翻译： 本文描述的是用标记的微胶囊颗粒标记白细胞和其它吞噬细胞的方法，包括将细胞与微胶粒颗粒一起孵育。 还描述了通过向受试者进行放射性标记的白细胞来检测感染的轨迹的方法，所述白细胞通过与标记的微粒颗粒孵育，随后扫描受试者以检测由颗粒发射的辐射的轨迹。
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9.发明授权Means of preparation and applications of liposomes containing high concentrations of entrapped ionic species 失效含有高浓度包埋离子物质的脂质体的制备和应用方法
公开(公告)号：US4310506A
公开(公告)日：1982-01-12
申请号：US123162
申请日：1980-02-20
CPC分类号： A61K51/1234 , A61K9/1278 , A61K2123/00 , Y10T428/2984
摘要： Unilamellar vesicles comprising a lipid bilayer, an ionophore being incorporated in said lipid bilayer, a chelating agent entrapped within the vesicles, and an effective amount of physiologically compatible cation bound to said chelating agent within the vesicles. Unilamellar vesicles are loaded by incubating the vesicles with the cation, terminating the incubation and recovering the loaded vesicles by chromatography. In some applications of the invention, the cation may be a radioactive tracer, in which case the vesicles can be administered to the human host and observed by scintillation techniques to produce a radioimage which is useful for diagnostic purposes.
摘要翻译： 包含脂质双层的单层囊泡，掺入所述脂质双层中的离子载体，包封在囊泡内的螯合剂，以及与囊泡内的所述螯合剂结合的有效量的生理上相容的阳离子。 通过将囊泡与阳离子孵育来加载单层囊泡，终止孵育并通过色谱法回收加载的囊泡。 在本发明的一些应用中，阳离子可以是放射性示踪剂，在这种情况下，囊泡可以施用于人宿主并通过闪烁技术观察以产生可用于诊断目的的放射性图像。
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10.发明申请SYSTEM AND METHOD FOR FACILITATING REFLECTOMETRIC DETECTION OF PHYSIOLOGIC ACTIVITY 有权用于促进生物活性反射检测的系统和方法公开(公告)号：US20130245437A1
公开(公告)日：2013-09-19
申请号：US13827555
申请日：2013-03-14
CPC分类号： A61B5/725 , A61B5/0006 , A61B5/0205 , A61B5/024 , A61B5/02405 , A61B5/0507 , A61B5/7257 , A61B5/7275 , A61B5/742 , A61B5/746
摘要： A method for remotely sensing cardiac-related data of an animal subject includes transmitting a RF signal to impinge on tissue of the subject, receiving a reflected portion of the RF signal, generating baseband data, filtering baseband data (e.g., including high pass filtering), performing waveform phase position determination, performing at least one auto-correlation of the waveform phase position determined data, determining periodicity of the auto-correlated data, and (i) computing heart rate using a maximum periodicity of the periodicity, or (ii) identifying abnormalities in cardiac function, such as may be indicated by temporal variations in heart rate and/or signal amplitude corresponding to cardiac activity. Multiple bandpass filtering schemes may be employed.
摘要翻译： 用于远程感测动物对象的心脏相关数据的方法包括：发送RF信号以撞击对象的组织，接收RF信号的反射部分，产生基带数据，滤波基带数据（例如，包括高通滤波） 执行波形相位位置确定，执行波形相位位置确定数据的至少一个自相关，确定自动相关数据的周期性，以及（i）使用周期的最大周期来计算心率，或者（ii） 识别心脏功能异常，例如可以通过对应于心脏活动的心率和/或信号幅度的时间变化来指示。 可以采用多个带通滤波方案。
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