摘要:
The present invention provides a process for producing trans-L-hydroxyproline, which comprises culturing a microorganism which is capable of decomposing amino acids other than trans-L-hydroxyproline but which is substantially incapable of decomposing trans-L-hydroxyproline in a culture medium containing collagen hydrolyzate, and recovering trans-L-hydroxyproline from the resulting culture.The process enhances the content of trans-L-hydroxyproline based on the total weight of amino acids contained in collagen hydrolyzate, and enables efficient production of trans-L-hydroxyproline which is useful as a starting material for the synthesis of medicines.
摘要:
Disclosed is a process for producing L-arginine by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of L-arginine and a vector DNA, culturing the transformant in a nutrient medium, accumulating L-arginine in the culture medium and recovering L-arginine therefrom.
摘要:
Disclosed are a process for producing 4-hydroxy-L-proline by the conversion of 4-hydroxy-2-oxoglutaric acid in an aqueous medium in the presence of an amino group donor and an enzyme source belonging to the genus Escherichia and capable of converting 4-hydroxy-2-oxoglutaric acid into 4-hydroxy-L-proline; and a microorganism which belongs to the genus Escherichia, holds a recombinant DNA incorporating a gene coding for .gamma.-glutamylkinase released from the feedback inhibition caused by L-proline, and contains the above enzyme source.
摘要:
L-arginine is produced by constructing a recombinant DNA composed of a vector DNA and a DNA fragment derived from chromosomal DNA of a microorganism belonging to the genus Corynebacterium or Brevibacterium and bearing genetic information relating to the synthesis of L-arginine-biosynthetic enzyme, introducing the recombinant DNA in a microorganism belonging to the genus Corynebacterium or Brevibacterium, culturing the microorganism in a medium, and recovering L-arginine accumulated in the culture broth.
摘要:
According to the present invention, a process for producing a practical cholesterol-reduced substance by converting cholesterol contained in foods and feeds to coprostanol having very low intestinal tract absorbability by utilizing enzymatic action is provided. Cholesterol in a cholesterol-containing substance such as meat, egg, milk, seafood and cooked processed foods containing the same, or feeds for animals, poultry and pisciculture, and the like, can be treated with three kinds of enzymes which are a cholesterol dehydrogenase having an optimum pH in a neutral pH range and 4-cholesten-3-one dehydrogenase and coprostan-3-one dehydrogenase each having an optimum pH in a weak acidic range, or microbial cells containing the enzymes, for converting the cholesterol to coprostanol to reduce the amount of the cholesterol.
摘要:
A process for producing alanine which comprises culturing in a medium a microorganism belonging to the genus Escherichia, Corynebacterium or Brevibacterium which has an L-alanine dehydrogenase activity and is capable of producing alanine, allowing the microorganism to produce and accumulate alanine in the culture medium, and recovering alanine from the culture medium.
摘要:
The present invention provides a gene expression regulatory DNA and a process for preparing a protein using the same.A DNA derived from the isocitrate lyase (ICL) gene of a coryneform bacterium regulates expression of a structural gene encoding a protein when incorporated into a vector DNA together with said structural gene and introduced into a host coryneform bacterium, and a useful protein can be efficiently produced using the DNA.
摘要:
Disclosed are recombinant plasmid vectors constructed of a plasmid autonomously replicable in cells of microorganisms belonging to the genus Corynebacterium or Brevibacterium and a DNA fragment containing a gene expressible by said microorganisms. The recombinant vector plasmids are autonomously replicable in glutamic acid--producing microorganisms and are useful to clone desired DNA fragments in such microorganisms.
摘要:
The present invention provides a method for industrially advantageously producing (S)-4-hydroxy-2-ketoglutaric acid and for producing compounds which are formed by biosynthesis from the precursor (S)-4-hydroxy-2-ketoglutaric acid, for example, for producing the compounds (2S,4S)-4-hydroxy-L-glutamic acid and (2S,4S)-4-hydroxy-L-proline, using a recombinant microorganism carrying a recombinant DNA harboring the DNA fragment encoding 4(S)-4-hydroxy-2-ketoglutaric acid aldolase gene.