Process for producing trans-L-hydroxyproline
    1.
    发明授权
    Process for producing trans-L-hydroxyproline 失效
    生产反式-L-羟脯氨酸的方法

    公开(公告)号:US5364775A

    公开(公告)日:1994-11-15

    申请号:US972824

    申请日:1992-11-06

    摘要: The present invention provides a process for producing trans-L-hydroxyproline, which comprises culturing a microorganism which is capable of decomposing amino acids other than trans-L-hydroxyproline but which is substantially incapable of decomposing trans-L-hydroxyproline in a culture medium containing collagen hydrolyzate, and recovering trans-L-hydroxyproline from the resulting culture.The process enhances the content of trans-L-hydroxyproline based on the total weight of amino acids contained in collagen hydrolyzate, and enables efficient production of trans-L-hydroxyproline which is useful as a starting material for the synthesis of medicines.

    摘要翻译: 本发明提供了生产反式-L-羟基脯氨酸的方法,该方法包括培养能够分解反式-L-羟基脯氨酸以外的氨基酸但基本上不能分解反式-L-羟脯氨酸的微生物,该培养基含有 胶原水解产物,并从所得培养物中回收反式-L-羟脯氨酸。 该方法基于胶原蛋白水解产物中所含的氨基酸的总重量,提高了反式-L-羟脯氨酸的含量,能有效地制备可用作药物合成起始原料的反式-L-羟脯氨酸。

    Process for producing 4-hydroxy-L-proline
    3.
    发明授权
    Process for producing 4-hydroxy-L-proline 失效
    生产4-羟基-L-脯氨酸的方法

    公开(公告)号:US5374542A

    公开(公告)日:1994-12-20

    申请号:US39482

    申请日:1993-04-26

    CPC分类号: C12P13/24 C12N9/12

    摘要: Disclosed are a process for producing 4-hydroxy-L-proline by the conversion of 4-hydroxy-2-oxoglutaric acid in an aqueous medium in the presence of an amino group donor and an enzyme source belonging to the genus Escherichia and capable of converting 4-hydroxy-2-oxoglutaric acid into 4-hydroxy-L-proline; and a microorganism which belongs to the genus Escherichia, holds a recombinant DNA incorporating a gene coding for .gamma.-glutamylkinase released from the feedback inhibition caused by L-proline, and contains the above enzyme source.

    摘要翻译: PCT No.PCT / JP91 / 01127 Sec。 371日期:1993年4月26日 102(e)日期1993年4月26日PCT 1991年8月26日PCT公布。 公开号WO93 / 04181 日期:1993年3月4日。未公开的是通过在氨基供体和属于下列物质的酶源的情况下在水性介质中转化4-羟基-2-氧代戊二酸来生产4-羟基-L-脯氨酸的方法 能够将4-羟基-2-氧代戊二酸转化为4-羟基-L-脯氨酸; 属于埃希氏杆菌属的微生物,含有由L-脯氨酸引起的反馈抑制释放的编码γ-谷氨酰基激酶的基因的重组DNA,并含有上述酶源。

    Process for producing L-arginine
    4.
    发明授权
    Process for producing L-arginine 失效
    L-精氨酸生产方法

    公开(公告)号:US5017482A

    公开(公告)日:1991-05-21

    申请号:US99798

    申请日:1987-09-22

    摘要: L-arginine is produced by constructing a recombinant DNA composed of a vector DNA and a DNA fragment derived from chromosomal DNA of a microorganism belonging to the genus Corynebacterium or Brevibacterium and bearing genetic information relating to the synthesis of L-arginine-biosynthetic enzyme, introducing the recombinant DNA in a microorganism belonging to the genus Corynebacterium or Brevibacterium, culturing the microorganism in a medium, and recovering L-arginine accumulated in the culture broth.

    摘要翻译: 通过构建由载体DNA构成的重组DNA和衍生自属于棒状杆菌属或短杆菌属的微生物的染色体DNA的DNA片段,并具有与L-精氨酸 - 生物合成酶的合成有关的遗传信息,引入L-精氨酸,引入L-精氨酸 属于棒杆菌属或短杆菌属的微生物的重组DNA,在培养基中培养微生物,并回收在培养液中积累的L-精氨酸。

    Process for producing alanine
    6.
    发明授权
    Process for producing alanine 失效
    丙氨酸生产方法

    公开(公告)号:US5559016A

    公开(公告)日:1996-09-24

    申请号:US170936

    申请日:1993-12-21

    CPC分类号: C12N9/0016 C12P13/06

    摘要: A process for producing alanine which comprises culturing in a medium a microorganism belonging to the genus Escherichia, Corynebacterium or Brevibacterium which has an L-alanine dehydrogenase activity and is capable of producing alanine, allowing the microorganism to produce and accumulate alanine in the culture medium, and recovering alanine from the culture medium.

    摘要翻译: 一种制备丙氨酸的方法,其包括在培养基中培养属于具有L-丙氨酸脱氢酶活性并具有L-丙氨酸脱氢酶活性并具有L-丙氨酸脱氢酶活性的埃希氏杆菌属,棒状杆菌属或短杆菌属的微生物,使微生物在培养基中产生和积累丙氨酸, 并从培养基中回收丙氨酸。

    Method for producing optically active compound
    10.
    发明授权
    Method for producing optically active compound 失效
    光学活性化合物的制造方法

    公开(公告)号:US06207427B1

    公开(公告)日:2001-03-27

    申请号:US09092063

    申请日:1998-06-05

    IPC分类号: C12P1324

    CPC分类号: C12P13/04 C12N9/88 C12P7/46

    摘要: The present invention provides a method for industrially advantageously producing (S)-4-hydroxy-2-ketoglutaric acid and for producing compounds which are formed by biosynthesis from the precursor (S)-4-hydroxy-2-ketoglutaric acid, for example, for producing the compounds (2S,4S)-4-hydroxy-L-glutamic acid and (2S,4S)-4-hydroxy-L-proline, using a recombinant microorganism carrying a recombinant DNA harboring the DNA fragment encoding 4(S)-4-hydroxy-2-ketoglutaric acid aldolase gene.

    摘要翻译: 本发明提供了一种工业上有利地生产(S)-4-羟基-2-酮戊二酸的方法,并且用于生产由前体(S)-4-羟基-2-酮戊二酸生物合成形成的化合物,例如, 使用携带携带有编码4(S)的DNA片段的重组DNA的重组微生物来制备化合物(2S,4S)-4-羟基-L-谷氨酸和(2S,4S)-4-羟基-L-脯氨酸, -4-羟基-2-酮戊二酸醛缩酶基因。