-
公开(公告)号：US5585242A
公开(公告)日：1996-12-17
申请号：US522623
申请日：1995-08-31
CPC分类号： G01N21/648 , B01L7/52 , C12Q1/6813 , C12Q1/6816 , C12Q1/6825 , C12Q1/6844 , C12Q1/686 , C12Q1/6862 , G01N21/6428 , G01N2021/6484
摘要： An apparatus and method for detecting amplified target nucleic acid is provided wherein the presence and concentration of amplified target is determined by total internal reflection over the course of the amplification reaction. A method and apparatus for detecting target nucleic acid is also provided wherein the presence and concentration of target is determined by total internal reflection and coupling of the target to the TIR element by scissile linkage. An improved immunoassay using total internal reflection and differential temperature cycling is further provided.
摘要翻译： 提供了用于检测扩增的靶核酸的装置和方法，其中扩增的靶的存在和浓度由扩增反应过程中的全内反射决定。 还提供了用于检测靶核酸的方法和装置，其中靶的存在和浓度由全内反射确定，并通过剪切连接将靶标与TIR元件偶联。 进一步提供使用全内反射和差示温度循环的改进的免疫测定。
-
公开(公告)号：US06210898B1
公开(公告)日：2001-04-03
申请号：US09397537
申请日：1999-09-16
申请人： Stanley R. Bouma , Julian Gordon , Joanell Hoijer , Cynthia Jou , James Rhoads
发明人： Stanley R. Bouma , Julian Gordon , Joanell Hoijer , Cynthia Jou , James Rhoads
IPC分类号： G01N2100
CPC分类号： C12Q1/6862 , C07K14/4712 , C12N15/10 , C12Q1/6813 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , G01N33/558 , Y10S436/807 , Y10S436/81 , C12Q2537/143
摘要： The invention relates to multiplex ligase chain reaction (LCR). Two or more putative target sequences are selected. For each one, a set of four probes is used simultaneously to amplify the putative sequence if it is present in the sample. Preferably, all the amplicons are labeled with a common label/hapten and, for each different target, with a unique label/hapten. The invention also relates to an immunochromatographic strip device and method employing a diagonal array of capture spots.
摘要翻译： 本发明涉及多重连接酶链反应（LCR）。 选择两个或多个推定的靶序列。 对于每一个，如果样品中存在，则同时使用一组四个探针来放大推定序列。 优选地，所有扩增子用公共标记/半抗原标记，并且对于每个不同的靶标，用唯一的标记/半抗原标记。 本发明还涉及使用捕获点的对角线阵列的免疫色谱条带装置和方法。
-
3.发明授权Method and reagent for detecting multiple nucleic acid sequences in a test sample 失效检测试样中多个核酸序列的方法和试剂
公开(公告)号：US5955268A
公开(公告)日：1999-09-21
申请号：US639224
申请日：1996-04-26
CPC分类号： C12Q1/6825
摘要： Provided herein are methods for detecting multiple target nucleic acid sequences in a test sample. Also provided is a hybridization platform useful for detecting multiple target sequences in a test sample. The hybridization platform comprises a solid support material having a defined pattern of capture probes immobilized thereon.
摘要翻译： 本文提供了用于在测试样品中检测多个靶核酸序列的方法。 还提供了可用于检测测试样品中的多个靶序列的杂交平台。 杂交平台包含具有固定在其上的捕获探针的限定图案的固体支持材料。
-
4.发明授权Nucleotide sequences useful as type specific probes, PCR primers and LCR probes for the amplification and detection of human papilloma virus, and related kits and methods 失效用作类型特异性探针的核苷酸序列，用于扩增和检测人乳头瘤病毒的PCR引物和LCR探针，以及相关试剂盒和方法
公开(公告)号：US5484699A
公开(公告)日：1996-01-16
申请号：US316293
申请日：1994-09-30
CPC分类号： C12Q1/708
摘要： Short nucleotide sequences of human papilloma virus useful for the determination of the presence and type of human papilloma virus present in a test sample. The sequences provided can be amplified by polymerase chain reaction or ligase chain reaction. The sequences provided also can be hybridized by standard slot-, dot- or replica-blot procedures. Methods and kits also are provided for the detection of human papilloma virus in a test sample and the determination of the type of human papilloma virus present in the test sample.
摘要翻译： 人乳头瘤病毒的短核苷酸序列可用于确定测试样品中存在的人乳头瘤病毒的存在和类型。 提供的序列可以通过聚合酶链反应或连接酶链反应进行扩增。 提供的序列也可以通过标准槽，点或复制印迹方法杂交。 还提供了用于在测试样品中检测人乳头瘤病毒并测定存在于测试样品中的人乳头状瘤病毒类型的方法和试剂盒。
-
公开(公告)号：US6068978A
公开(公告)日：2000-05-30
申请号：US859552
申请日：1997-05-20
CPC分类号： B01L7/52 , B01L3/502 , G01N2035/00237 , G01N2035/00366 , G01N2035/00376 , G01N2035/00752 , G01N35/1095 , Y10S435/808
摘要： Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a one or two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection chamber to form the reaction/detection unit. A first heating element of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element and amplified target nucleic acid is immobilized on a support in the detection chamber. Microprocessor control controls the heat applied by the second element independently of the heat applied by the first element. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Images are captured and digitized and subjected to statistical analysis to improve the accuracy of the method.
摘要翻译： 提供了扩增和检测核酸的方法，装置，装置和试剂盒。 该装置是与反应/检测单元结合操作的一层或两层热循环装置。 将样品装载到装置的反应室中，然后将其与检测室配合以形成反应/检测单元。 热循环装置的第一加热元件将所需的温度施加到反应/检测装置以扩增样品中的靶核酸。 然后将反应混合物通过第二加热元件转移到检测室，并将扩增的靶核酸固定在检测室中的载体上。 微处理器控制独立于第一元件施加的热量来控制由第二元件施加的热量。 与该装置相关的检测系统检测并分析固定的扩增的核酸靶。 图像被捕获并数字化并进行统计分析以提高该方法的准确性。
-
公开(公告)号：US5858652A
公开(公告)日：1999-01-12
申请号：US331298
申请日：1994-10-28
申请人： Thomas G. Laffler , Stanley R. Bouma , Uwe Spies
发明人： Thomas G. Laffler , Stanley R. Bouma , Uwe Spies
CPC分类号： C12Q1/686
摘要： A method and kits for amplifying and detecting target nucleic acid sequences in a sample is disclosed. The method employs primers which have reactive pair members linked to them. The reactive pair members can be attached to a solid phase and/or detected by labeled conjugate.
摘要翻译： 公开了用于扩增和检测样品中靶核酸序列的方法和试剂盒。 该方法使用具有与其连接的反应对成员的引物。 活性对成员可以连接到固相和/或通过标记的缀合物检测。
-
公开(公告)号：US5645801A
公开(公告)日：1997-07-08
申请号：US516728
申请日：1995-08-18
申请人： Stanley R. Bouma , Ronald A. Coules , Julian Gordon , Eric B. Shain , Natalie A. Solomon , Peter Zaun
发明人： Stanley R. Bouma , Ronald A. Coules , Julian Gordon , Eric B. Shain , Natalie A. Solomon , Peter Zaun
CPC分类号： B01L7/52 , Y10S435/81
摘要： Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then sealably mated with a detection chamber to form a sealed reaction/detection unit that is virtually irreversibly closed. One or more heating elements of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Kits include the reaction/detection units and reagents for amplification.
摘要翻译： 提供了用于扩增和检测核酸的方法，装置，装置和试剂盒。 该装置是与反应/检测单元结合操作的热循环装置。 将样品装载到装置的反应室中，然后与检测室密封地配合，以形成几乎不可逆地封闭的密封的反应/检测单元。 热循环装置的一个或多个加热元件对反应/检测装置施加所需的温度以放大样品中的靶核酸。 然后将反应混合物转移到检测室，并将扩增的靶核酸固定在检测室中的载体上。 与该装置相关的检测系统检测并分析固定的扩增的核酸靶。 试剂盒包括用于扩增的反应/检测单元和试剂。
-
公开(公告)号：US5006473A
公开(公告)日：1991-04-09
申请号：US230217
申请日：1988-08-09
IPC分类号： G01N33/561 , G01N33/58
CPC分类号： G01N33/561 , G01N33/586 , Y10S436/829
摘要： The electrophoresis method of the present invention employs a media which contains uniformly dispersed liposomes of phospholipid, or combinations of phospholipid and neutral lipid, which contain chromogenic materials or dye precursers. After electrophoresis of a test sample, the liposomes are lysed and the chromogen or dye material released. The chromogen or dye can be any signal producing substance including a chromogenic agent, and enzyme, a fluorogenic agent, or a chemiluminescent agent, but a detectable signal occurs only where the staining material is in close proximity to specific enzymes, effectors, analytes, or other color-inducing agents which have migrated through the gel during electrophoresis.
摘要翻译： 本发明的电泳方法采用含有均匀分散的磷脂的脂质体或含有显色物质或染料前体的磷脂和中性脂质的组合的培养基。 在测试样品电泳后，将脂质体裂解，并释放色原体或染料。 色原或染料可以是包括显色剂，酶，荧光剂或化学发光剂的任何信号产生物质，但是仅当染色材料紧密接近特定酶，效应物，分析物或 在电泳期间已经迁移通过凝胶的其它引诱剂。
-
公开(公告)号：US5869252A
公开(公告)日：1999-02-09
申请号：US769176
申请日：1996-12-18
申请人： Stanley R. Bouma , Julian Gordon , Joanell Hoijer , Cynthia Jou , James Rhoads
发明人： Stanley R. Bouma , Julian Gordon , Joanell Hoijer , Cynthia Jou , James Rhoads
CPC分类号： C12Q1/6862
摘要： The invention relates to multiplex ligase chain reaction (LCR). Two or more purative target sequences are selected. For each one, a set of four probes is used simultaneously to amplify the putative sequence if it is present in the sample. Preferably, all the amplicons are labeled with a common label/hapten and, for each different target, with a unique label/hapten. The invention also relates to an immunochromatographic strip device and method employing a diagonal array of capture spots.
摘要翻译： 本发明涉及多重连接酶链反应（LCR）。 选择两个或更多个纯化靶序列。 对于每一个，如果样品中存在，则同时使用一组四个探针来放大推定序列。 优选地，所有扩增子用公共标记/半抗原标记，并且对于每个不同的靶标，用唯一的标记/半抗原标记。 本发明还涉及使用捕获点的对角线阵列的免疫色谱条带装置和方法。
-
10.发明授权
公开(公告)号：US5858732A
公开(公告)日：1999-01-12
申请号：US697404
申请日：1996-08-23
CPC分类号： C12Q1/6851 , C12Q1/6862 , C12Q1/703 , C12Q1/706 , C12Q1/6834
摘要： The present invention provides a method of detecting the amount of a target sequence which may be present in a test sample. The method uses an aggregate primer series, which comprises at least two primer sets, in an amplification reaction to detect the relative concentration of a target sequence which may be present in a test sample. The primer sets have different sensitivities and hybridize with sub-target sequences which are different regions of the target sequence. The method generally comprises cycling a test sample suspected of containing a target sequence, an aggregate primer series, and means necessary for performing an amplification reaction; and detecting any amplified sub-target sequences. Based on a qualitative detection of the amplified sub-target sequences generated by individual primer sets, the relative quantity of the target sequence can be determined.
摘要翻译： 本发明提供了检测可能存在于测试样品中的靶序列的量的方法。 该方法在扩增反应中使用包含至少两个引物组的聚集引物序列，以检测可能存在于测试样品中的靶序列的相对浓度。 引物组具有不同的灵敏度并与靶序列的不同区域的亚靶序列杂交。 该方法通常包括循环怀疑含有靶序列的测试样品，聚集引物序列和进行扩增反应所需的手段; 并检测任何扩增的亚靶序列。 基于由各引物组产生的扩增子靶序列的定性检测，可以确定目标序列的相对量。
-
-
-
-
-
-
-
-
-
搜索结果
12 条记录 (耗时 0.03 秒)
