公开(公告)号：US5173626A

公开(公告)日：1992-12-22

申请号：US712541

申请日：1991-06-10

申请人： Tsuneaki Kudou ,  Naoko Nakamura

发明人： Tsuneaki Kudou ,  Naoko Nakamura

IPC分类号： G01R31/28 ,  G06F11/267 ,  H01L21/66 ,  H03K3/037 ,  H03K17/693

CPC分类号： G06F11/2236 ,  H03K17/693 ,  H03K3/0372

摘要： An improved flip-flop with a scan path comprises a front page circuit driven in response to a clock signal and multiplexers for receiving a data signal and a scan test signal. Each of the multiplexers comprises a single stage of FETs connected in series between a power source and a ground. This arrangement can remarkably improve an operation frequency.

摘要翻译： 具有扫描路径的改进的触发器包括响应时钟信号驱动的前置电路和用于接收数据信号和扫描测试信号的多路复用器。 每个复用器包括在电源和地之间串联连接的单级FET。 这种布置可以显着提高操作频率。

公开(公告)号：US20090035750A1

公开(公告)日：2009-02-05

申请号：US12134420

申请日：2008-06-06

申请人： Koji Hashimoto ,  Keiko Ito ,  Naoko Nakamura ,  Hideki Horiuchi ,  Michie Hashimoto ,  Osamu Sato

发明人： Koji Hashimoto ,  Keiko Ito ,  Naoko Nakamura ,  Hideki Horiuchi ,  Michie Hashimoto ,  Osamu Sato

IPC分类号： C12Q1/70 ,  C07H21/00

CPC分类号： C12Q1/708

摘要： Provided is a nucleic acid primer for LAMP amplification for use in the detection of human papilloma virus and identification of its genotype. The present invention also provides a method of detecting human papilloma virus and identifying its genotype, includes a step of amplifying the nucleic acid chains in a sample in LAMP reaction by using multiple primers including at least one primer selected from the nucleic acid primers according to the present invention and a step of detecting presence of amplified products after the amplification reaction and identifying their genotypes.

摘要翻译： 提供了用于LAMP扩增的核酸引物，用于检测人乳头瘤病毒并鉴定其基因型。 本发明还提供了一种检测人乳头状瘤病毒并鉴定其基因型的方法，包括通过使用包含至少一种引物的多个引物来扩增LAMP反应中的核酸链的步骤，该引物选自根据本发明的核酸引物 本发明和扩增反应后检测扩增产物的存在并鉴定其基因型的步骤。

公开(公告)号：US20080215272A1

公开(公告)日：2008-09-04

申请号：US11792304

申请日：2005-10-14

申请人： Katsumasa Fujita ,  Satoshi Kawata ,  Naoko Nakamura ,  Osamu Nakamura ,  Minoru Kobayashi

发明人： Katsumasa Fujita ,  Satoshi Kawata ,  Naoko Nakamura ,  Osamu Nakamura ,  Minoru Kobayashi

IPC分类号： G06F15/00

CPC分类号： G01N21/6458 ,  G01N21/6408 ,  G01N21/6428 ,  G01N2201/0697 ,  G02B21/16

摘要： To increase spatial resolution by observing a sample based on saturated fluorescence components. A fluorescence microscope according to the present invention includes: a laser light source 10 emitting laser light as excitation light; an objective lens 13 focusing the laser light and applying the focused laser light to a sample 14; a detector 22 detecting fluorescence generated in the sample 14 with the laser light; and a stage 15 scanning the sample 14 while moving the sample 14 relative to the laser light, wherein the laser light is applied to the sample with varying intensities such that saturation of fluorescence occurs at the maximum intensity of the laser light, and fluorescence is detected with the detector in accordance with intensity of the laser light, and the sample is observed based on the saturation components of fluorescence.

摘要翻译： 通过观察基于饱和荧光成分的样品来增加空间分辨率。 根据本发明的荧光显微镜包括：发射激光作为激发光的激光光源10; 聚焦激光并将聚焦的激光施加到样品14的物镜13; 用激光检测样品14中产生的荧光的检测器22; 以及在样品14相对于激光移动的同时扫描样品14的阶段15，其中激光以不同的强度施加到样品，使得在激光的最大强度下发生荧光饱和，并且检测到荧光 与检测器根据激光的强度，并且基于荧光的饱和分量观察样品。

公开(公告)号：US20060018961A1

公开(公告)日：2006-01-26

申请号：US11236640

申请日：2005-09-28

申请人： Naoko Nakamura ,  Naoki Kameyama ,  Akihito Iwasa ,  Yukinari Iwata

发明人： Naoko Nakamura ,  Naoki Kameyama ,  Akihito Iwasa ,  Yukinari Iwata

IPC分类号： A61K9/20 ,  A61K31/366

CPC分类号： A61K9/2018 ,  A61K9/0056 ,  A61K9/2013 ,  A61K9/2027 ,  A61K31/366 ,  A61K45/06 ,  A61K2300/00

摘要： A compression formed preparation that rapidly disintegrates in the mouth or in an aqueous solvent, which imparts an excellent feeling during administration, and maintains a suitable hardness required for handling such as distribution and the like, and a method for manufacturing the compression formed preparation. The present invention provides a rapidly disintegrating compression formed preparation comprising one or more compounds selected from gluconolactones and pullulans added to the pharmaceutical base. The pharmaceutical base is preferably a saccharide and particularly preferably a sugar alcohol and starch syrup. The compression formed preparation is prepared by compressing granules obtained by granulation of particles comprising preparation assistants in addition to the pharmaceutical base in a solution of gluconolactone or pullulan dissolved in an aqueous solvent or together with gluconolactone or pullulan in an aqueous solvent.

摘要翻译： 一种在口腔或水性溶剂中快速崩解的压制成型制剂，其在给药期间赋予优异的感觉，并保持诸如分布等的处理所需的合适的硬度，以及压缩成型制剂的制造方法。 本发明提供了包含一种或多种选自加入到药物基质中的葡糖酸内酯和支链淀粉的化合物的快速崩解的压制成形制剂。 药物基质优选为糖类，特别优选糖醇和淀粉糖浆。 通过将溶解在水性溶剂中的葡萄糖酸内酯或支链淀粉的溶液中的制剂助剂以外的制剂助剂颗粒压制成颗粒而得到的压缩成型制剂，或者与葡萄糖酸内酯或支链淀粉一起在水性溶剂中。

公开(公告)号：US07951541B2

公开(公告)日：2011-05-31

申请号：US12851675

申请日：2010-08-06

申请人： Naoko Nakamura ,  Keiko Ito

发明人： Naoko Nakamura ,  Keiko Ito

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6844 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2531/101

摘要： Kits for use in a method of detecting an amplification product by hybridizing it with a probe, the amplification product is amplified from a target nucleic acid with the primers, including placing F3, F2 and F1 regions in this order from a 5′ terminal side and B3c, B2c and B1c regions in this order from a 3′ terminal side, and additionally an FP region in the region from the F2 to F1 regions and/or a BPc region in the region from the B2c to B1c regions in the target nucleic acid, determining the respective regions in such a manner that the FP and F2 regions and/or the BPc and B2c regions have an unoverlapping region of at least 10 bases or more and overlapping regions of 10 bases or less, and designing the primers according to the regions.

摘要翻译： 用于通过与探针杂交来检测扩增产物的方法的试剂盒，使用引物从靶核酸扩增扩增产物，包括从5'末端侧依次放置F3，F2和F1区， B3c，B2c和B1c区域，另外在靶核酸中的从B2c到B1c区域的F2到F1区和/或BPc区的区域中的FP区域 以这样的方式确定各个区域，使得FP和F2区域和/或BPc和B2c区域具有至少10个碱基以上的重叠区域和10个碱基以下的重叠区域，并且根据 地区。

公开(公告)号：US07919252B2

公开(公告)日：2011-04-05

申请号：US12341295

申请日：2008-12-22

申请人： Naoko Nakamura ,  Koji Hashimoto

发明人： Naoko Nakamura ,  Koji Hashimoto

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6813

摘要： A method of detecting a target nucleic acid sequence comprising providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion, providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface, reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid, and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid.

摘要翻译： 一种检测靶核酸序列的方法，包括提供用于测量的茎 - 环结构化核酸，其中所述核酸包含位于两端的互补序列部分和其间的靶序列部分以及由双链部分形成的双链部分 位于两个末端的互补序列部分和剩余的环状单链部分的杂交，提供具有与靶序列部分互补的序列的探针核酸，其中探针核酸的一端被固定在固体基质表面上，使 用于与探针核酸进行测量的核酸以将用于测量的核酸的靶序列部分特异性杂交到探针核酸，并检测与探针核酸杂交的用于测量的核酸的存在或不存在。

公开(公告)号：US20090117544A1

公开(公告)日：2009-05-07

申请号：US11624814

申请日：2007-01-19

申请人： Naoko Nakamura ,  Keiko Ito

发明人： Naoko Nakamura ,  Keiko Ito

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6844 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2531/101

摘要： A method of designing primers for use in a method of detecting an amplification product by hybridizing it with a probe, the amplification product is amplified from a target nucleic acid with the primers, including placing F3, F2 and F1 regions in this order from a 5′ terminal side and Bc, B2c and B1c regions in this order from a 3′ terminal side, and additionally an FP region in the region from the F2 to F1 regions and/or a BPc region in the region from the B2c to B1c regions in the target nucleic acid, determining the respective regions in such a manner that the FP and F2 regions and/or the BPc and B2c regions have an unoverlapping region of at least 10 bases or more and overlapping regions of 10 bases or less, and designing the primers according to the regions.

摘要翻译： 一种设计引物用于通过与探针杂交来检测扩增产物的方法的引物的方法，用引物从靶核酸扩增扩增产物，其中包括将F3，F2和F1区域从5 '端子侧和Bc，B2c和B1c区域，另外在从F2到F1区域的区域中的FP区域和/或从B2c到B1c区域的区域中的BPc区域 靶核酸，以使得FP和F2区域和/或BPc和B2c区域具有至少10个碱基以上的不重叠区域和10个碱基以下的重叠区域的方式来确定各个区域，并且设计 引物根据区域。

公开(公告)号：US5714382A

公开(公告)日：1998-02-03

申请号：US463242

申请日：1995-06-05

申请人： Akira Yanai ,  Naoko Nakamura ,  Susumu Matsuda

发明人： Akira Yanai ,  Naoko Nakamura ,  Susumu Matsuda

IPC分类号： C12N1/21 ,  A61K38/21 ,  A61P37/00 ,  C07K1/22 ,  C07K14/52 ,  C07K14/555 ,  C07K14/56 ,  C12N5/10 ,  C12N15/09 ,  C12N15/21 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91

CPC分类号： C07K14/555

摘要： A synthetic plasmid in which DNA encoding protein of a feline interferon is integrated, a transformant obtainable by the transformation of a host cell by the use of the synthetic plasmid and a feline interferon having a biological activity given by a protein carrying a specific amino acid sequence, a feline interferon gene encoding the feline interferon, a feline interferon precursor comprised of a cleavable peptide or a signal peptide being linked to the N terminal of the feline interferon, a feline interferon precursor gene encoding the feline interferon precursor and a method for producing the feline interferon, which are applied to the mass production of a feline interferon to be used as a remedy for feline viral disease and tumor.

摘要翻译： 将编码猫干​​扰素的蛋白质的DNA整合的合成质粒，通过使用合成质粒转化宿主细胞而获得的转化体和具有由具有特定氨基酸序列的蛋白质赋予的生物活性的猫干扰素 ，编码猫干扰素的猫干扰素基因，由可切割肽或与猫干扰素的N末端连接的信号肽构成的猫干扰素前体，编码猫干扰素前体的猫干扰素前体基因，以及制备 猫干扰素，其被用于大量生产猫干扰素，用作猫病毒性疾病和肿瘤的补救剂。

公开(公告)号：US20190194607A1

公开(公告)日：2019-06-27

申请号：US13982661

申请日：2012-01-30

申请人： Akira Yuo ,  Kumiko Saeki ,  Naoko Nakamura ,  Satoko Matsuyama ,  Miwako Nishio ,  Koichi Saeki ,  Mamoru Hasegawa

发明人： Akira Yuo ,  Kumiko Saeki ,  Naoko Nakamura ,  Satoko Matsuyama ,  Miwako Nishio ,  Koichi Saeki ,  Mamoru Hasegawa

IPC分类号： C12N5/071 ,  C12Q1/02

CPC分类号： C12N5/067 ,  C12N5/0672 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/237 ,  C12N2501/39 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2506/02 ,  C12N2506/45 ,  C12Q1/025 ,  G01N33/5014 ,  G01N33/5067

摘要： The problem is to produce functional liver cells usable in testing the metabolism and toxicity of a drug, from pluripotent stem cells. The solution includes a method for producing highly functional liver cells using pluripotent stem cells, comprising, from pluripotent stem cells, acquiring primitive endoderm derived from the pluripotent stem cells by a process involving steps (A) and (B), from the primitive endoderm, acquiring liver precursor cells by a process involving step (C), and, from the liver precursor cells, acquiring the highly functional liver cells by a process involving step (D): (A) culturing under serum-free and feeder-free conditions; (B) culturing in the presence of albumin and at least one kind of cytokine; (C) culturing in the presence of SHH or an SHH agonist and at least one kind of cytokine; and (D) culturing and maturing in the presence of at least one kind of cytokine.

公开(公告)号：US08673595B2

公开(公告)日：2014-03-18

申请号：US13340572

申请日：2011-12-29

申请人： Naoko Nakamura ,  Koji Hashimoto ,  Nobuhiro Gemma

发明人： Naoko Nakamura ,  Koji Hashimoto ,  Nobuhiro Gemma

IPC分类号： C12P19/34

CPC分类号： C12Q1/6837 ,  C12Q1/6809 ,  C12Q1/6853 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q2565/514

摘要： One embodiment is related to a method of analyzing plural samples. The method includes amplifying a plurality of samples using a first primer and second primer, wherein the first primer includes a tag sequence having a sequence different from a sample to one another and wherein a second primer used in pair with the first primer in independent reaction systems for the respective samples to obtain an amplified product in which the tag sequence is introduced, mixing amplified products obtained in the plurality of reaction systems, making the mixed amplified product react with a nucleic acid probe immobilized on a substrate, and detecting the amount of hybridization that has occurred.

摘要翻译： 一个实施例涉及分析多个样本的方法。 该方法包括使用第一引物和第二引物扩增多个样品，其中第一引物包含具有不同于样品的序列的标签序列，并且其中与独立反应体系中的第一引物成对配对的第二引物 对于各样品获得其中引入标签序列的扩增产物，混合在多个反应体系中获得的扩增产物，使得混合的扩增产物与固定在底物上的核酸探针反应，并检测杂交的量 已经发生了。