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公开(公告)号:US08846414B2
公开(公告)日:2014-09-30
申请号:US12569725
申请日:2009-09-29
申请人: Ramakrishna Sista , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Michael G. Pollack , Richard B. Fair , Allen E. Eckhardt
发明人: Ramakrishna Sista , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Michael G. Pollack , Richard B. Fair , Allen E. Eckhardt
IPC分类号: G01N33/553 , G01N33/68 , G01N33/543
CPC分类号: G01N33/6893 , G01N33/54366 , G01N2333/4712 , G01N2333/58 , G01N2800/32 , Y10T436/25
摘要: The present invention provides for the detection of cardiac markers on a droplet actuator. An aspect provides a method of assaying a cardiac marker in a biological sample from a subject, the method including providing a droplet actuator, loading the biological sample and assay reagents on the droplet actuator, executing droplet operations to create sample droplets from the sample and reagent droplets from the reagents on the droplet actuator, and executing droplet operations using the sample droplets and reagent droplets to produce a detectable signal indicative of the quantity of the cardiac marker in the biological sample. Still other aspects are provided.
摘要翻译: 本发明提供了在液滴致动器上检测心脏标志物。 一方面提供了从受试者的生物样品中测定心脏标志物的方法,所述方法包括提供液滴致动器,在液滴致动器上装载生物样品和测定试剂,执行液滴操作以从样品和试剂产生样品液滴 来自液滴致动器上的试剂的液滴,以及使用样品液滴和试剂液滴执行液滴操作,以产生指示生物样品中心脏标志物量的可检测信号。 还提供了其他方面。
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公开(公告)号:US09046514B2
公开(公告)日:2015-06-02
申请号:US12524495
申请日:2008-02-11
申请人: Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Richard B. Fair
发明人: Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Richard B. Fair
CPC分类号: G01N33/54326 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L2200/025 , B01L2200/028 , B01L2200/0647 , B01L2200/0673 , B01L2200/141 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0427 , B01L2400/043 , C12M25/01 , C12M25/16 , G01N27/44717 , G01N27/44786 , G01N33/54386 , G01N35/0098 , G01N2035/1046
摘要: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator (200) comprising: (i) a substrate (210) comprising electrodes (214) arranged for conducting droplet operations on a surface; (ii) a starting droplet (218/222) comprising: (1) one or more magnetically responsive beads (220); (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone, (224); (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet (218) comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet (222) substantially lacking the magnetically responsive beads.
摘要翻译: 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤:(a)提供液滴致动器(200),包括:(i)包括布置用于在表面上进行液滴操作的电极(214)的基底(210) (ii)起始液滴(218/222),包括:(1)一个或多个磁响应珠(220); (2)物质的起始量; 和(3)起始体积; (b)将所述一个或多个磁响应珠磁性地固定在距离目标液滴分离区一段距离的位置处(224); (c)进行一个或多个液滴操作,其包括液滴分割操作,所述液滴分割操作被选择以分割组合的液滴以产生一组液滴,其包括:(i)液滴(218),其基本上包含所有一个或多个磁响应珠粒, 物质的数量相对于起始浓度; 和(ii)基本上不含磁响应珠的液滴(222)。
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公开(公告)号:US20100068764A1
公开(公告)日:2010-03-18
申请号:US12524495
申请日:2008-02-11
申请人: Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt
发明人: Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt
CPC分类号: G01N33/54326 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L2200/025 , B01L2200/028 , B01L2200/0647 , B01L2200/0673 , B01L2200/141 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0427 , B01L2400/043 , C12M25/01 , C12M25/16 , G01N27/44717 , G01N27/44786 , G01N33/54386 , G01N35/0098 , G01N2035/1046
摘要: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator (200) comprising: (i) a substrate (210) comprising electrodes (214) arranged for conducting droplet operations on a surface; (ii) a starting droplet (218/222) comprising: (1) one or more magnetically responsive beads (220); (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone, (224); (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet (218) comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet (222) substantially lacking the magnetically responsive beads.
摘要翻译: 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤:(a)提供液滴致动器(200),包括:(i)包括布置用于在表面上进行液滴操作的电极(214)的基底(210) (ii)起始液滴(218/222),包括:(1)一个或多个磁响应珠(220); (2)物质的起始量; 和(3)起始体积; (b)将所述一个或多个磁响应珠磁性地固定在距离目标液滴分离区一段距离的位置处(224); (c)进行一个或多个液滴操作,其包括液滴分割操作,所述液滴分割操作被选择以分割组合的液滴以产生一组液滴,其包括:(i)液滴(218),其基本上包含所有一个或多个磁响应珠粒, 物质的数量相对于起始浓度; 和(ii)基本上不含磁响应珠的液滴(222)。
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公开(公告)号:US20110104747A1
公开(公告)日:2011-05-05
申请号:US12921256
申请日:2009-03-09
申请人: Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人: Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
IPC分类号: G01N1/40
CPC分类号: B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.
摘要翻译: 提供了将珠子集中在流体装置上的液滴和/或负载珠粒中的方法,其中包括将珠粒集中在液滴中的方法,所述方法包括:(a)提供液滴致动器,其包括:(i) 内部液滴操作量; 和(ii)内部容积外部的储存器; (iii)建立在从储存器延伸到内部容积中的液体路径中的液滴; (b)在储存器中的液滴部分中提供磁响应珠; (c)通过所述液体路径将所述磁响应珠磁吸引到位于所述内部容积中的所述液滴的所述部分中; 和(d)在内部容积中形成包含一个或多个磁响应珠的液滴。
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公开(公告)号:US09675972B2
公开(公告)日:2017-06-13
申请号:US12921256
申请日:2009-03-09
申请人: Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人: Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
CPC分类号: B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.
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公开(公告)号:US08562807B2
公开(公告)日:2013-10-22
申请号:US12747231
申请日:2008-12-10
申请人: Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt , Vamsee K. Pamula
发明人: Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt , Vamsee K. Pamula
IPC分类号: G01N27/453
CPC分类号: G01N27/44743 , B01L3/0268 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/061 , B01L2200/0647 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B01L2400/0427
摘要: Droplet actuators for conducting droplet operations, such as droplet transport and droplet dispensing, are provided. In one embodiment, the droplet actuator may include a substrate including, droplet operations electrodes arranged for conducting droplet operations on a surface of the substrate; and reference electrodes associated with the droplet operations electrodes and extending above the surface of the substrate. Other embodiments of droplet actuators and methods of loading and using such droplet actuators are also provided.
摘要翻译: 提供用于进行液滴操作的滴液致动器,例如液滴传输和液滴分配。 在一个实施例中,液滴致动器可以包括基板,其包括布置成在基板的表面上进行液滴操作的液滴操作电极; 以及与液滴操作电极相关并在衬底表面上方延伸的参考电极。 还提供了液滴致动器的其他实施例以及加载和使用这种液滴致动器的方法。
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公开(公告)号:US20100140093A1
公开(公告)日:2010-06-10
申请号:US12615609
申请日:2009-11-10
IPC分类号: C25B7/00
CPC分类号: G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a bead-containing droplet with a reduced concentration of a substance is provided, the method including providing a droplet microactuator including a droplet having a starting volume and including one or more beads and a starting concentration and starting quantity of the substance. The method further includes conducting one or more droplet operations to merge a wash droplet with the droplet provided in the above step to yield a combined droplet and including one or more droplet operations to divide the combined droplet to yield a set of droplets including a droplet including substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration and a droplet which is substantially lacking in the beads.
摘要翻译: 本发明涉及液滴表面改性和洗涤。 根据一个实施例,提供了一种提供具有降低的物质浓度的含珠粒的液滴的方法,所述方法包括提供包括具有起始体积并包括一个或多个珠粒和起始浓度的液滴的液滴微致动器,并起始浓度 物质的数量。 该方法还包括进行一个或多个液滴操作以将洗涤液滴与上述步骤中提供的液滴合并以产生组合液滴并且包括一个或多个液滴操作以分开组合的液滴以产生包括液滴的一组液滴,包括液滴,包括 基本上所有的一种或多种珠粒,并且物质相对于起始浓度降低,而珠子中基本上不含有液滴。
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公开(公告)号:US20070243634A1
公开(公告)日:2007-10-18
申请号:US11639736
申请日:2006-12-15
IPC分类号: G01N33/543
CPC分类号: G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a droplet microactuator comprising a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
摘要翻译: 本发明涉及液滴表面改性和洗涤。 根据一个实施例,提供了一种提供与物质浓度降低的表面接触的液滴的方法,其中所述方法包括:(a)提供液滴微致动器,其包括与包含起始浓度的液滴的表面 并开始物质的起始体积; (b)进行一个或多个液滴操作以将洗涤液滴与步骤(a)中提供的液滴合并以产生组合液滴; 和(c)进行一个或多个液滴操作以分开组合的液滴以产生一组液滴,其包括:(i)与所述表面接触的液滴相对于起始浓度具有降低的物质浓度; 和(ii)与表面分离的液滴。
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公开(公告)号:US08541176B2
公开(公告)日:2013-09-24
申请号:US12113385
申请日:2008-05-01
IPC分类号: G01N33/53
CPC分类号: G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of modifying a surface on a droplet microactuator is provided, wherein the method includes executing one or more droplet operations to bring a droplet comprising a surface-modifying agent into contact with the surface. According to another embodiment, a droplet microactuator is provided and includes a sample or reagent immobilized on a surface thereof and arranged such that a droplet on the droplet microactuator may contact the surface. According to yet another embodiment, a method of removing a substance from a surface to which the substance is bound is provided, the method including conducting one or more droplet operations to contact a droplet with the surface, the droplet comprising a solution for eluting the substance from the surface
摘要翻译: 本发明涉及液滴表面改性和洗涤。 根据一个实施例,提供了修改液滴微致动器上的表面的方法,其中所述方法包括执行一个或多个液滴操作以使包含表面改性剂的液滴与表面接触。 根据另一个实施例,提供液滴微致动器,并且包括固定在其表面上的样品或试剂,并且布置成使得液滴微致动器上的液滴可接触表面。 根据另一个实施方案,提供从物质结合的表面除去物质的方法,所述方法包括进行一个或多个液滴操作以使液滴与表面接触,所述液滴包含用于洗脱物质的溶液 从表面
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公开(公告)号:US08313895B2
公开(公告)日:2012-11-20
申请号:US12615609
申请日:2009-11-10
IPC分类号: C12Q1/00
CPC分类号: G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a bead-containing droplet with a reduced concentration of a substance is provided, the method including providing a droplet microactuator including a droplet having a starting volume and including one or more beads and a starting concentration and starting quantity of the substance. The method further includes conducting one or more droplet operations to merge a wash droplet with the droplet provided in the above step to yield a combined droplet and including one or more droplet operations to divide the combined droplet to yield a set of droplets including a droplet including substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration and a droplet which is substantially lacking in the beads.
摘要翻译: 本发明涉及液滴表面改性和洗涤。 根据一个实施例,提供了一种提供具有降低的物质浓度的含珠粒的液滴的方法,所述方法包括提供包括具有起始体积并包括一个或多个珠粒和起始浓度的液滴的液滴微致动器,并起始浓度 物质的数量。 该方法还包括进行一个或多个液滴操作以将洗涤液滴与上述步骤中提供的液滴合并以产生组合液滴并且包括一个或多个液滴操作以分开组合的液滴以产生包括液滴的一组液滴,包括液滴,包括 基本上所有的一种或多种珠粒,并且物质相对于起始浓度降低,而珠子中基本上不含有液滴。
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