公开(公告)号：US20250035553A1

公开(公告)日：2025-01-30

申请号：US18785572

申请日：2024-07-26

Applicant: Waters Technologies Corporation

Inventor： Kevin Wyndham

IPC: G01N21/64 ,  G01N33/68

Abstract: Disclosed herein are methods for the multiplexed characterization of analytes using dye-tagged affinity molecules. The DTAMs, useful in conjunction with liquid chromatography systems, allow for the parallel quantitation and characterization of attributes of one or more target analytes from complex solutions.

公开(公告)号：US20240367072A1

公开(公告)日：2024-11-07

申请号：US18654384

申请日：2024-05-03

Applicant: Waters Technologies Corporation

Inventor： Kevin Wyndham ,  Beatrice Muriithi ,  Oksana Tchoul ,  Yeliz Tunc Sarisozen ,  Mingcheng Xu ,  Matthew Lauber

IPC: B01D15/38 ,  B01J20/26 ,  B01J20/28

Abstract: The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 micrometers and being functionalized with an immunoglobulin-binding protein. The functionalized particles of the present disclosure can be used to purify a range of immunoglobulins with affinity to the immunoglobulin-binding protein.

公开(公告)号：US20220122699A1

公开(公告)日：2022-04-21

申请号：US17500489

申请日：2021-10-13

Applicant: Waters Technologies Corporation

Inventor： Thomas S. McDonald ,  Kevin Wyndham ,  Johannes Vissers ,  Edouard S. P. Bouvier

IPC: G16H10/40 ,  G01N33/68 ,  G16H50/80

Abstract: The present disclosure relates to methods and workflows for processing and analyzing clinical samples (e.g., nasal swabs, throat swabs, plasma samples, urine, etc.) In general, the methods and workflows involve the detection of an infectious state and are improved over contemporary methods due to increased throughput with improved resolution of positive results.

公开(公告)号：US12180581B2

公开(公告)日：2024-12-31

申请号：US17166460

申请日：2021-02-03

Applicant: Waters Technologies Corporation

Inventor： Stephen J. Shiner ,  Cheryl Boissel ,  Matthew A. Lauber ,  Kevin Wyndham

IPC: C23C16/02 ,  C09D143/04 ,  C23C14/04 ,  C23C14/12 ,  C23C14/16 ,  C23C14/58 ,  G01N30/56 ,  G01N30/60

Abstract: A chromatographic device including a coated metallic frit is disclosed. The coating is provided over the fluid exposed surfaces of the frit, thereby covering metallic surfaces to prevent interaction with an analyte in the flowstream. This technology relates to the use of vapor deposition coated frit(s) in a liquid flow path for improved chromatography. More specifically, this technology relates to liquid chromatographic devices for separating analytes in a sample having coated frit(s) within an uncoated metallic fluidic flow path (i.e., the column tube or channel is formed of stainless steel, titanium (pure or alloyed), or some mixture of stainless steel and titanium) and does not include the coating applied to the frit.

公开(公告)号：US20240361318A1

公开(公告)日：2024-10-31

申请号：US18647863

申请日：2024-04-26

Applicant: Waters Technologies Corporation

Inventor： Kevin Wyndham ,  Beatrice Muriithi ,  Martin Gilar ,  Yeliz Tunc Sarisozen

IPC: G01N33/569 ,  G01N33/53 ,  G01N33/543 ,  G01N33/74

CPC classification number: G01N33/56983 ,  G01N33/5308 ,  G01N33/54313 ,  G01N33/54386 ,  G01N33/74 ,  G01N2333/015 ,  G01N2333/62 ,  G01N2469/10

Abstract: The present technology is directed to methods of preparing affinity chromatographic columns using streptavidin-conjugated particles and biotinylated affinity agents (i.e., biotinylated oligonucleotides, biotinylated antibodies or biotinylated antigen-binding fragments thereof). The methods of the present technology can be utilized to perform affinity capture assays in a high-throughput, efficient manner. Further, the affinity chromatographic columns disclosed herein are customizable due to the ability to utilize any biotinylated affinity agent. The methods herein utilize an on-line loading (i.e., loading the column with biotinylated affinity agent via a liquid chromatography system).

公开(公告)号：US20220082532A1

公开(公告)日：2022-03-17

申请号：US17471449

申请日：2021-09-10

Applicant: Waters Technologies Corporation

Inventor： Amit Patel ,  Moon Chul Jung ,  Susan Rzewuski ,  Thomas H. Walter ,  Matthew A. Lauber ,  Kevin Wyndham ,  Mathew DeLano ,  Jennifer Simeone

IPC: G01N30/16 ,  G01N30/86 ,  G01N30/72 ,  G01N30/74

Abstract: The present disclosure is directed to methods for evaluating system inertness, such as the inertness of a LC or other fluidic system. Some methods are directed to tests wherein the column has been removed prior to injecting a sample including a positive (e.g., metal reacting moiety) control into the system. Some methods can include: (1) repeatedly injecting the sample into a system, the system comprising: fluidic paths wherein interior surfaces of the fluidic paths define wetted surfaces, and wherein at least a portion of the wetted surfaces of the fluidic flow path are coated with an inert coating, wherein the inert coating is inert to at least one analyte in the sample; (2) detecting a value associated with the positive control; and (3) analyzing values associated with the detected positive control to determine system inertness.

公开(公告)号：US20210349061A1

公开(公告)日：2021-11-11

申请号：US17308637

申请日：2021-05-05

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Kevin Wyndham ,  Bonnie Alden ,  Dominic Foley ,  David Morrissey

IPC: G01N30/56 ,  G01N30/88 ,  G01N30/72

Abstract: The present disclosure relates to the use of vapor deposition coated flow paths for improved chromatography and sample analysis using liquid chromatography-mass spectrometry (LC/MS) or liquid chromatography-optical detection (LC/UV). More specifically, this technology relates to separating and quantitation of analytes (e.g., phospho prodrugs and its phosphorylated metabolites) from a sample matrix (e.g., mammalian blood, plasma) using chromatographic devices and fluidic systems having coated flow paths. The LC-MS or LC-UV techniques provide improved recovery, peak shape and dynamic range in the analysis of the prodrug and its metabolites.

公开(公告)号：US20240359161A1

公开(公告)日：2024-10-31

申请号：US18647811

申请日：2024-04-26

Applicant: Waters Technologies Corporation

Inventor： Kevin Wyndham ,  Beatrice Muriithi ,  Oksana Tchoul ,  Yeliz Tunc Sarisozen ,  MingCheng Xu ,  Matthew Lauber

IPC: B01J20/289 ,  B01D15/22 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/32

CPC classification number: B01J20/289 ,  B01D15/22 ,  B01D15/3809 ,  B01J20/261 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3274

Abstract: The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 micrometers and being functionalized with streptavidin. The streptavidin functionalized particles of the present disclosure can be bound to biotinylated antibodies, biotinylated antigen-binding fragments thereof, or biotinylated oligonucleotides to create customizable affinity chromatography columns. That is, the functionalized nonporous polymer particles can be packed in a stainless steel, titanium, or other metal or metal alloy column for affinity high pressure liquid chromatography (HPLC).

公开(公告)号：US11874256B2

公开(公告)日：2024-01-16

申请号：US17471449

申请日：2021-09-10

Applicant: Waters Technologies Corporation

Inventor： Amit Patel ,  Moon Chul Jung ,  Thomas H. Walter ,  Matthew A. Lauber ,  Kevin Wyndham ,  Mathew DeLano ,  Jennifer Simeone

IPC: G01N30/16 ,  G01N30/72 ,  G01N30/74 ,  G01N30/86 ,  G01N30/06 ,  G01N30/02

CPC classification number: G01N30/16 ,  G01N30/72 ,  G01N30/74 ,  G01N30/8637 ,  G01N30/06 ,  G01N2030/027

Abstract: The present disclosure is directed to methods for evaluating system inertness, such as the inertness of a LC or other fluidic system. Some methods are directed to tests wherein the column has been removed prior to injecting a sample including a positive (e.g., metal reacting moiety) control into the system. Some methods can include: (1) repeatedly injecting the sample into a system, the system comprising: fluidic paths wherein interior surfaces of the fluidic paths define wetted surfaces, and wherein at least a portion of the wetted surfaces of the fluidic flow path are coated with an inert coating, wherein the inert coating is inert to at least one analyte in the sample; (2) detecting a value associated with the positive control; and (3) analyzing values associated with the detected positive control to determine system inertness.

公开(公告)号：US20230182114A1

公开(公告)日：2023-06-15

申请号：US18056434

申请日：2022-11-17

Applicant: Waters Technologies Corporation

Inventor： Yeliz Tunc Sarisozen ,  Nicole L. Lawrence ,  Darryl W. Brousmiche ,  MingCheng Xu ,  Kevin Wyndham

IPC: B01J20/289 ,  B01J20/287 ,  B01J20/28 ,  B01J20/10 ,  B01D15/34

CPC classification number: B01J20/289 ,  B01D15/34 ,  B01J20/103 ,  B01J20/287 ,  B01J20/2808 ,  B01J20/28052 ,  B01J20/28083 ,  B01J20/28085 ,  B01J2220/54 ,  B01J2220/64 ,  B01J2220/66

Abstract: The present disclosure is directed to surface modified materials such as stationary phase materials for performing size exclusion chromatography. Aspects of the present disclosure feature materials surface modified with a moiety including a polyethylene glycol (PEG) functionality and a moiety comprising a diol functionality. Such surface modified materials exhibit a reduced propensity for ionic and hydrophobic secondary interactions.