利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

73 条记录 (耗时 0.035 秒)

    Methods for analyzing inter-cellular phenomena
    1.
    发明授权
    Methods for analyzing inter-cellular phenomena 有权
    分析细胞间现象的方法

    公开(公告)号：US07450229B2

    公开(公告)日：2008-11-11

    申请号：US11123610

    申请日：2005-05-04

    申请人： William E. Ortyn ,  David A. Basiji ,  David H. Lynch

    发明人： William E. Ortyn ,  David A. Basiji ,  David H. Lynch

    IPC分类号： G01J3/28 ,  G01J3/51

    CPC分类号： G01N33/505 ,  G01J3/2889 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/5005 ,  G01N2015/1006 ,  G01N2015/1497 ,  G01N2021/6421 ,  G01N2021/6441 ,  G06K9/00127

    摘要： Aspects of the present invention encompass the collection of multispectral images from a population of objects, and the analysis of the collected images to measure at least one characteristic of the population, using photometric and/or morphometric features identifiable in the collection of images. In an exemplary application, the objects are biological cells. In a particularly preferred, but not limiting implementation, the plurality of images for each individual object are collected simultaneously. In an empirical study, the characteristic being measured involves the synapse between conjugated cells. The conjugated cells may represent a subpopulation of the overall population of objects that were imaged. In a particularly preferred, yet not limiting embodiment, the present invention enables the quantization of the redistribution of cellular molecules due to the conjugation of different biological cells. Significantly, such quantization is not feasible with standard microscopy and flow cytometry.

    摘要翻译： 本发明的方面包括从对象群体收集多光谱图像，以及使用在图像集合中可识别的光度和/或形态测量特征来分析收集的图像以测量群体的至少一个特征。 在示例性应用中，对象是生物细胞。 在特别优选但不是限制的实现中，用于每个单独对象的多个图像被同时收集。 在实证研究中，所测量的特征涉及共轭细胞之间的突触。 共轭细胞可以代表成像对象的总体群体的亚群。 在特别优选的但不是限制性的实施方案中，本发明使得由于不同生物细胞的共轭而能够量化细胞分子的再分布。 值得注意的是，这种量化在标准显微镜和流式细胞术中是不可行的。

    Multipass cavity for illumination and excitation of moving objects
    5.
    发明授权
    Multipass cavity for illumination and excitation of moving objects 有权
    多光腔用于照明和激发移动物体

    公开(公告)号：US06707551B2

    公开(公告)日：2004-03-16

    申请号：US10355653

    申请日：2003-01-29

    申请人： William E. Ortyn ,  David A. Basiji ,  Keith L. Frost ,  N. Robert McRuer

    发明人： William E. Ortyn ,  David A. Basiji ,  Keith L. Frost ,  N. Robert McRuer

    IPC分类号： G01N2100

    CPC分类号： G02B27/126 ,  G01J3/2803 ,  G01J3/2823 ,  G01N15/1434 ,  G01N15/147 ,  G01N21/031 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/85 ,  G01N2015/0294 ,  G01N2015/1006 ,  G01N2015/1472 ,  G01N2015/1486 ,  G02B7/28

    摘要： An illumination system for increasing a light signal from an object passing through a reflection cavity. The reflection cavity is disposed between spaced-apart, opposed first and second surfaces disposed on opposite sides of a moving stream of objects. A light collection system is disposed substantially orthogonal to a plane passing through the surfaces and the stream so as to collect light that is scattered from or emitted by the objects as they pass through a field of view disposed between the first and second surfaces. A beam of light from a laser source is directed through the stream of moving objects in a direction nearly orthogonal to the stream (but slightly inclined) and lying in the plane that extends through the surfaces and the stream. Due to the reflection angle and the distance between the stream and the first surface, the point at which the light reflected from the first surface intersects the stream on a second pass is displaced from where it passed though the stream on its initial pass. The light is reflected back and forth between the surfaces a plurality of times, illuminating a different portion of the field of view with each pass until, having ranged over the field of view, the light exits the reflection cavity. The “recycling” of the light beam in this manner substantially improves the SNR of the detection system by increasing an average illumination intensity experienced by the objects in the stream.

    摘要翻译： 一种用于增加来自穿过反射腔的物体的光信号的照明系统。 反射腔设置在位于物体的移动物流的相对侧上的间隔开的相对的第一和第二表面之间。 光采集系统设置成基本上垂直于穿过表面和流的平面，以便收集当物体经过设置在第一表面和第二表面之间的视野时从物体散射或发射的光。 来自激光源的光束被引导通过几何垂直于流（但稍微倾斜）并且位于延伸穿过表面和流的平面中的移动物体流。 由于反射角度和流与第一表面之间的距离，从第一表面反射的光在第二遍上与流相交的点从其在其初始通过时通过流的位置偏移。 光在表面之间反复反射多次，每次通过照射视场的不同部分，直到在视场范围内，光离开反射腔。 以这种方式，光束的“回收”通过增加物流在物流中经历的平均照明强度，大大提高了检测系统的信噪比。

    Imaging and analyzing parameters of small moving objects such as cells
    7.
    发明授权
    Imaging and analyzing parameters of small moving objects such as cells 有权
    成像和分析细胞等小移动物体的参数

    公开(公告)号：US06473176B2

    公开(公告)日：2002-10-29

    申请号：US09820434

    申请日：2001-03-29

    申请人： David A. Basiji ,  William E. Ortyn

    发明人： David A. Basiji ,  William E. Ortyn

    IPC分类号： G01J312

    CPC分类号： G01N21/6456 ,  G01J3/2803 ,  G01J3/2823 ,  G01N15/147 ,  G01N21/05 ,  G01N21/6428 ,  G01N2015/0294 ,  G01N2015/1006 ,  G01N2015/1472 ,  G01N2015/1486 ,  G01N2021/052 ,  G01N2021/058 ,  G02B7/28 ,  G02B27/1013 ,  G02B27/126 ,  G02B27/144 ,  G02B27/145 ,  G02B27/148

    摘要： Light from an object such as a cell moving through an imaging system is collected and dispersed so that it can be imaged onto a detector. The light can be emitted from a luminous object or can be light from a light source that has been scattered or not absorbed by the object or can include a light emission by one or more probes within or on the object. Multiple objects passing through the imaging system can be imaged, producing both scatter images and dispersed images at different locations on one or more detectors. In one embodiment, the detector is divided into a plurality of zones so that each of a plurality of different images are spectrally dispersed onto a different zone of the detector.

    摘要翻译： 来自诸如移动通过成像系统的细胞的物体的光被收集和分散，使得其可以成像到检测器上。 光可以从发光物体发射，或者可以是来自被物体散射或不被吸收的光源的光，或者可以包括物体内或物体上的一个或多个探针的发光。 通过成像系统的多个物体可被成像，在一个或多个检测器上的不同位置产生分散图像和分散图像。 在一个实施例中，检测器被分成多个区域，使得多个不同图像中的每一个被频谱分散到检测器的不同区域上。