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    Chemically-enhanced primer compositions, methods and kits
    6.
    发明授权
    Chemically-enhanced primer compositions, methods and kits 有权
    化学增强底漆组合物，方法和试剂盒

    公开(公告)号：US08703925B2

    公开(公告)日：2014-04-22

    申请号：US13397626

    申请日：2012-02-15

    申请人： Linda Lee ,  Sam Woo ,  Peter Ma

    发明人： Linda Lee ,  Sam Woo ,  Peter Ma

    IPC分类号： C07H21/02 ,  C07H21/04 ,  B01L3/00 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2525/125

    摘要： A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

    摘要翻译： 提供化学增强的引物，其包含带负电荷的部分（NCM），具有a）非核酸酶抗性核苷酸间连接的寡核苷酸序列或b）至少一个核酸酶抗性核苷酸间连接。 化学增强引物可用于测序和片段分析。 还提供了用于合成化学增强引物的方法以及制备用于测序的DNA的方法，测序DNA的方法和含有化学增强的引物的试剂盒。 测序DNA的方法可以包括使扩增反应产物与其中过量扩增引物被核酸酶降解并且化学增强引物基本上未降解的组合物接触。

    Wide Waistband and Pant Supporting Undergarment
    9.
    发明申请
    Wide Waistband and Pant Supporting Undergarment 有权
    宽腰带和裤支持内衣

    公开(公告)号：US20140196196A1

    公开(公告)日：2014-07-17

    申请号：US14147751

    申请日：2014-01-06

    申请人： Linda Lee

    发明人： Linda Lee

    IPC分类号： A41B9/14

    CPC分类号： A41B9/14 ,  A41B9/02 ,  A41F7/00

    摘要： The present invention provides underwear that are configured for use with pants that are worn below the waistline of a user. The underwear may comprise a wide waistband that is configured to cover from one-third to one-half the total length of the underwear, whereby the waistband occupies an area from just above to just below the waistline of a user. Alternatively, the underwear may comprise hooks that are adapted to hold and maintain a pair of pants at a fixed distance from the waistline, and pockets for holding a user's belongings. The underwear may be made of an elastic material, such as nylon, and may be decorated with a variety of aesthetic colors and designs to suit the tastes of a user.

    摘要翻译： 本发明提供了被配置为与佩戴在使用者腰围下方的裤子一起使用的内衣。 内衣可以包括宽腰带，其被配置为覆盖内衣的总长度的三分之一到一半，由此腰带占据从使用者的腰围的正上方到正下方的区域。 或者，内衣可以包括适于保持和维持与腰围固定距离的一条裤子的钩子，以及用于保持使用者的物品的口袋。 内衣可以由诸如尼龙的弹性材料制成，并且可以用各种美学颜色和设计来装饰以适应使用者的口味。

    METHODS OF LABELING NUCLEIC ACIDS WITH ENERGY TRANSFER DYES
    10.
    发明申请
    METHODS OF LABELING NUCLEIC ACIDS WITH ENERGY TRANSFER DYES 有权
    用能量转运标记标记核酸的方法

    公开(公告)号：US20070212709A1

    公开(公告)日：2007-09-13

    申请号：US11618663

    申请日：2006-12-29

    申请人： Linda Lee ,  Sandra Spurgeon ,  Barnett Rosenblum

    发明人： Linda Lee ,  Sandra Spurgeon ,  Barnett Rosenblum

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C07H21/04 ,  C07D311/82 ,  C07D311/90 ,  C07D405/12 ,  C07D405/14 ,  C07H21/00 ,  C09B11/04 ,  C09B11/24 ,  C09B23/083 ,  C09B69/00 ,  C12Q1/6818 ,  C12Q1/6869 ,  C12Q2563/107 ,  C12Q2535/101 ,  C12Q2565/1015

    摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.

    摘要翻译： 提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。 这些连接器有助于在能量转移染料中的供体和受体染料之间的能量的有效转移。 用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基， 基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基 不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。