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    Method for pretreatment of microbial samples
    3.
    发明授权
    Method for pretreatment of microbial samples 有权
    微生物样品预处理方法

    公开(公告)号：US08975060B2

    公开(公告)日：2015-03-10

    申请号：US13833872

    申请日：2013-03-15

    Applicant: Qvella Corporation

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Robert Maaskant ,  Tino Alavie

    IPC: C12N1/00

    CPC classification number: C12Q1/6848 ,  B01L3/5021 ,  B01L3/50215 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/1833 ,  C12Q1/02 ,  C12Q1/6806 ,  C12Q2523/307 ,  C12Q2527/125 ,  C12Q2527/137

    Abstract: Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included, for collecting the microbial cells adjacent to a liquid interface formed by the cushioning liquid, upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed to prior to extraction of the pretreated sample.

    Abstract translation: 提供了用于预处理含有微生物细胞的样品的方法和装置。 在一些实施方案中，样品的预处理通过在样品预处理容器内，非微生物细胞（例如血细胞）中的初始选择性裂解进行，随后将样品离心以除去所得碎片并浓缩微生物 细胞。 可以包括不混溶和致密的缓冲液体，用于在预处理容器离心时收集由缓冲液形成的液体界面附近的微生物细胞。 在除去大量的上清液后，重新悬浮收集的微生物细胞并重新建立液体界面，可以除去至少一部分剩余的悬浮液，而基本不除去缓冲液体。 可以在提取预处理样品之前进行一个或多个中间洗涤循环。

    Systems and methods for classification of microbial cells grown in microcolonies
    4.
    发明授权
    Systems and methods for classification of microbial cells grown in microcolonies 有权

    公开(公告)号：US12247197B2

    公开(公告)日：2025-03-11

    申请号：US18012433

    申请日：2021-06-25

    Applicant: QVELLA CORPORATION

    Inventor： Samad Talebpour ,  Anna Khimchenko ,  Maryam Asadishekari ,  Stephen Wesley Leonard

    IPC: G06K9/62 ,  C12N1/20 ,  G02B21/36 ,  G06T5/50 ,  G06T5/70 ,  G06V10/147 ,  G06V10/774 ,  G06V10/82 ,  G06V20/69

    Abstract: Systems and methods are provided for classifying microbial cells according to morphological features of microcolonies. A dark-field objective is employed to acquire a dark-field image of a microcolony during a microcolony growth phase that is characterized by phenotypic expression of microcolony morphological features which evolve with time and are differentiated among classes of microbial cell types. The dark-field image is processed to classify the microcolony according to two or more microbial cell types, such as Gram status and/or speciation. The dark-field objective may have a numerical aperture selected to facilitate the imaging of microcolony morphological features, residing, for example, between 0.15 and 0.35. A set of dark-field images of a microcolony may be collected during the microcolony growth phase and processed to classify the microcolony. Classification may be performed according to a temporal ordering of the dark-field images, for example, using a recurrent neural network.

    METHODS AND COMPOSITIONS FOR THE SELECTIVE LYSIS OF BLOOD CELLS AND SEPARATION OF MICROBIAL CELLS
    7.
    发明申请
    METHODS AND COMPOSITIONS FOR THE SELECTIVE LYSIS OF BLOOD CELLS AND SEPARATION OF MICROBIAL CELLS 有权

    公开(公告)号：US20210208128A1

    公开(公告)日：2021-07-08

    申请号：US17057088

    申请日：2019-05-24

    Applicant: QVELLA CORPORATION

    Inventor： Samad TALEBPOUR ,  Aye Aye KHINE ,  Vilcy PARMAR ,  Sukhdev MANKU ,  Alaleh SAMIEI

    IPC: G01N33/49 ,  G01N35/00

    Abstract: Methods and compositions are provided for the selective lysis of eukaryotic cells and the separation of microbial cells. Blood cells and/or other eukaryotic cells in a sample, may be selectively lysed by adding, to the sample, a blood lysis reagent including saponin and an alkaline buffer, and optionally sodium polyanethole sulfonate and a non-ionic surfactant, thereby forming a mixture, and agitating the mixture. Microbial cells in the mixture may then be separated, for example, using a separation method such as centrifugation or filtration, and optionally detected or cultured in growth media. Blood lysis reagent compositions are provided that are suitable for preserving the intactness of microbial cells upon mixing with the sample. In example embodiments in which the sample is a blood sample, the blood lysis reagent composition may be selected to avoid or reduce the presence of visible blood debris upon centrifugation or filtration.

    Methods of targeted antibiotic susceptibility testing
    9.
    发明授权
    Methods of targeted antibiotic susceptibility testing 有权

    公开(公告)号：US11866762B2

    公开(公告)日：2024-01-09

    申请号：US17240093

    申请日：2021-04-26

    Applicant: QVELLA CORPORATION

    Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

    IPC: C12Q1/18 ,  C12Q1/689

    CPC classification number: C12Q1/18 ,  C12Q1/689 ,  C12Q2600/106 ,  C12Q2600/158

    Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production of m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

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