公开(公告)号：US5272058A

公开(公告)日：1993-12-21

申请号：US479691

申请日：1990-02-13

申请人： William A. Petri, Jr. ,  Jonathan I. Ravdin

发明人： William A. Petri, Jr. ,  Jonathan I. Ravdin

IPC分类号： A61K39/00 ,  C07K14/44 ,  C07K16/20 ,  C12Q1/68 ,  G01N33/569 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/566 ,  A61K35/14 ,  C07K3/00 ,  C07K13/00 ,  C07K15/00 ,  C07K17/00

CPC分类号： C07K16/20 ,  C07K14/44 ,  C12Q1/6893 ,  G01N33/56905 ,  A61K39/00 ,  Y10S435/961 ,  Y10S435/975

摘要： A simple immunoassay method is provided which distinguishes pathogenic from nonpathogenic forms Entamoeba histolytica in biological samples. This assay utilizes monoclonal antibody preparations which are specific for designated epitopes of the 170 kd subunit of the Gal/GalNAc lectin. When pathogenic forms, specifically, are to be detected, at least one antibody which is immunospecific for an epitope unique to the forms of the 170 kd lectin found in pathogenic strains is used in the assay. The invention further includes monoclonal antibodies which are immunospecific for epitopes 1-3 of the 170 kd subunit of the Gal/GalNAc lectin of either pathogenic or nonpathogenic forms and to monoclonal antibodies specifically immunoreactive with epitopes unique to nonpathogenic derived 170 kd subunit, as well as purified forms of the Gal/GalNAc lectin from both pathogenic and nonpathogenic forms.

摘要翻译： 提供了简单的免疫测定方法，其在生物样品中区分致病与非病原体形式的溶组织内阿米巴。 该测定使用对Gal / GalNAc凝集素的170kd亚基的指定表位特异的单克隆抗体制剂。 当要检测到特异性的致病形式时，在测定中使用至少一种针对在致病菌株中发现的170kd凝集素形式特有的表位具有免疫特异性的抗体。 本发明还包括针对致病性或非致病性形式的Gal / GalNAc凝集素的170kd亚基的表位1-3具有免疫特异性的单克隆抗体以及与非致病性衍生的170kd亚基特有的表位特异性免疫反应的单克隆抗体，以及 Gal / GalNAc凝集素的纯化形式来源于致病和非病原体形式。

公开(公告)号：US4923801A

公开(公告)日：1990-05-08

申请号：US37938

申请日：1987-04-13

申请人： Barry J. Marshall ,  Richard L. Guerrant

发明人： Barry J. Marshall ,  Richard L. Guerrant

IPC分类号： C12Q1/24 ,  C12Q1/58

CPC分类号： C12Q1/58 ,  C12Q1/24

摘要： A method of enrichment and isolation of urease producing organisms from a contaminated specimen by first homogenizing the contaminated specimen in water, then introducing the homogenized contaminated specimen into a solution of urea in an acid, wherein some of the organisms are killed by the acidic medium and remaining organisms are protected from acid attack by creating a protective ammonia by breaking down the urea, and plating the remaining organisms onto a medium which contains antibiotics inhibitory to some of the remaining organisms, but not inhibitory to organisms to be isolated.

摘要翻译： 通过首先将被污染的样品在水中均质化，然后将均质化的污染标本引入尿素在酸中的溶液中，从而将污染的样品富集和分离出来，从而将一些生物体被酸性介质杀死， 通过分解尿素来产生保护性氨，并将剩余的生物体电镀到含有抑制一些剩余生物体的抗生素但不抑制待分离的生物体的培养基上，从而保护剩余的生物体免受酸侵蚀。

公开(公告)号：US4812040A

公开(公告)日：1989-03-14

申请号：US31212

申请日：1987-03-27

申请人： R. Kenneth Marcus ,  W. W. Harrison

发明人： R. Kenneth Marcus ,  W. W. Harrison

IPC分类号： G01N21/73 ,  G01J3/10

CPC分类号： G01N21/73

摘要： Sample material is sputtered from an orifice in a disc mounted in a hollow cathode. A plasma plume is ejected from the orifice and the material sputtered from the smaple is transported directly into the base of the plasma plume. Collisions with particles in the plasma plume excite the sputtered material. Light emission and absorption from the plume are measured and ions in the plume are measured. A chamber surrounding the plasma plume is maintained at about 1 torr. About 15 cc's per minute of argon are supplied to the hollow cathode at 2 torr. The power supply supplies about 200 volts at about 0.10 amps. Low energy argon ions strick the disc at the end of the cathode tube and sputter atoms off the aperture. Atoms collide with particles in the plasma causing excitation, photon emission and ionization of atoms which are measured by optical and mass spectrometers.

摘要翻译： 样品材料从安装在中空阴极中的盘中的孔口溅射。 等离子体羽流从喷口喷射出来，从喷雾器溅出的材料直接输送到等离子体羽流的底部。 与等离子体羽流中的颗粒碰撞激发溅射的材料。 测量羽流的发光和吸收，并测量羽流中的离子。 围绕等离子体羽流的室保持在约1托。 将约15cc /分钟的氩气以2托供应到中空阴极。 电源以约0.10安培供应约200伏特。 低能量的氩离子在阴极管的末端刺入光盘，并将光源溅射出来。 原子与等离子体中的颗粒碰撞，引起光子和质谱仪测量的原子的激发，光子发射和电离。

公开(公告)号：US4781692A

公开(公告)日：1988-11-01

申请号：US30986

申请日：1987-03-27

申请人： Janine C. Jagger ,  Richard D. Pearson ,  Patrice G. Guyenet

发明人： Janine C. Jagger ,  Richard D. Pearson ,  Patrice G. Guyenet

IPC分类号： A61M5/32 ,  A61M25/06 ,  A61M5/00

CPC分类号： A61M25/0637 ,  A61M25/0631 ,  A61M5/3243

摘要： A flexible plastic catheter extends from a distal end of a rigid plastic housing. An elastomeric seal at the distal end of the housing tightly fits within the housing and grips in its bore a stylet. The stylet has a sharp distal end and an enlarged proximal end which receives a distal end of a plastic tube within the housing. The stylet is slideable within the elastic seal. In an extended position, the stylet distal end extends beyond a distal end of the catheter and the proximal end abuts the elastomeric seal. In a retracted position, the elastomeric seal grips the catheter near its distal end and the proximal end abuts a constriction in the proximal end of the housing. In that retracted position, the tubing, the stylet and the catheter form a fluid flow path.

摘要翻译： 柔性塑料导管从刚性塑料外壳的远端延伸。 在壳体的远端处的弹性体密封件紧密地配合在壳体内并且在其孔中夹持探针。 探针具有尖锐的远端和扩大的近端，其接收在壳体内的塑料管的远端。 探针可在弹性密封内滑动。 在延伸位置，探针远端延伸超过导管的远端，并且近端邻接弹性体密封。 在缩回位置，弹性密封件将导管靠近其远端夹持，并且近端邻近壳体的近端中的收缩部。 在缩回位置，管道，探针和导管形成流体流动路径。

公开(公告)号：US4446064A

公开(公告)日：1984-05-01

申请号：US218075

申请日：1980-12-19

申请人： Joseph Larner ,  Kang Cheng ,  Gail Galasko

发明人： Joseph Larner ,  Kang Cheng ,  Gail Galasko

IPC分类号： A61K35/34 ,  C07G7/00

CPC分类号： A61K35/34 ,  Y10S530/841

摘要： An insulin mediator substance produced by the process comprising: contacting muscle tissue with insulin; deproteinizing the muscle tissue; removing the major nucleotides from the deproteinized muscle tissue; chromatographing the so-treated product on a Sephadex G-25 column using 0.05 N formic acid; and recovering the fraction wherein the major 230-nm absorbance peak corresponds with the ninhydrin-positive peak.

摘要翻译： 通过该方法产生的胰岛素介体物质包括：使肌肉组织与胰岛素接触; 去蛋白肌肉组织; 去除脱蛋白肌肉组织中的主要核苷酸; 使用0.05N甲酸在Sephadex G-25柱上色谱处理如此处理的产物; 并回收其中主要的230nm吸收峰对应于茚三酮正峰的级分。

公开(公告)号：US5047508A

公开(公告)日：1991-09-10

申请号：US42193

申请日：1987-04-21

申请人： John C. Herr ,  Mark Sigman ,  William M. Sutherland

发明人： John C. Herr ,  Mark Sigman ,  William M. Sutherland

IPC分类号： C07K16/18 ,  C12N5/20

CPC分类号： C07K16/18

摘要： A monoclonal antibody MHS-5 which specifically binds to a seminal vesicle specific antigen, SVSA, wherein the seminal vesicle specific antigen is secreted by the principal cells of the seminal vesicle epithelium. The method for producing this monoclonal antibody is also disclosed.

摘要翻译： 特异性结合精囊特异性抗原SVSA的单克隆抗体MHS-5，其中精囊细胞特异性抗原由精囊上皮的主要细胞分泌。 还公开了该单克隆抗体的制备方法。

公开(公告)号：US4840076A

公开(公告)日：1989-06-20

申请号：US161384

申请日：1988-02-22

申请人： Clifford E. Brubaker ,  Colin A. McLaurin

发明人： Clifford E. Brubaker ,  Colin A. McLaurin

IPC分类号： A61G5/02 ,  B62B5/04

CPC分类号： A61G5/023 ,  A61G5/025 ,  A61G5/1008 ,  A61G5/1032 ,  A61G5/1035 ,  Y10T74/1556

摘要： An apparatus for propelling a wheeled vehicle which has a hand-manipulated lever that is connected to a clutch assembly. The clutch assembly has a drum that is rotatably connected to the vehicle and brake bands that are pivotably connected to the lower end of the lever. An actuator rod connected to the lever causes inward movement of the bands to engage the clutch. Once engaged the vehicle can be propelled in which ever direction the lever is being rotated.

摘要翻译： 一种用于推进轮式车辆的装置，其具有连接到离合器组件的手动操纵杆。 离合器组件具有可旋转地连接到车辆的滚筒和可枢转地连接到杠杆的下端的制动带。 连接到杠杆的致动器杆引起带的向内移动以接合离合器。 一旦接合，车辆可以被推进，杆在哪个方向上被转动。

公开(公告)号：US4741998A

公开(公告)日：1988-05-03

申请号：US741601

申请日：1985-06-05

申请人： John C. Herr ,  Mark Sigman ,  William M. Sutherland

发明人： John C. Herr ,  Mark Sigman ,  William M. Sutherland

IPC分类号： G01N33/53 ,  C07K1/22 ,  C07K14/005 ,  C07K14/195 ,  C07K16/00 ,  C07K16/18 ,  C12N5/00 ,  C12N5/10 ,  C12N15/00 ,  C12P21/00 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577 ,  C07K15/00 ,  G01N33/535

CPC分类号： C07K16/18 ,  Y10S435/81 ,  Y10S435/948 ,  Y10S435/975 ,  Y10S436/808

摘要： A new probe for forensic analysis of sexual assaults is disclosed. The probe is a monoclonal antibody to a new protein marker, MHS-5, in semen. Also disclosed is the hybridoma producing the antibody as well as an assay utilizing the antibody for forensic analysis of criminal evidence.

摘要翻译： 披露了一项关于性攻击法医分析的新探索。 探针是针对新的蛋白质标记物MHS-5在精液中的单克隆抗体。 还公开了产生抗体的杂交瘤以及利用该抗体进行刑事证据法医分析的试验。

公开(公告)号：US4581011A

公开(公告)日：1986-04-08

申请号：US605678

申请日：1984-04-30

申请人： Fern G. Campbell

发明人： Fern G. Campbell

IPC分类号： A61M1/28 ,  A61M1/00

CPC分类号： A61M1/28

摘要： A manual administration set for acute peritoneal dialysis in infants and adults is disclosed which has a minimum of connections so as to minimize risk of infection and includes a heating means for patient comfort and performance of more efficient dialysis. The prepackaged set up for infants and small children contains volutroles within the tubing for the measurement of small volumes to be used in dialysis. The set up is capable of handling from one container up to four containers and will have sufficient tubing length to allow for any amount of movement of the child or the adult patient.

摘要翻译： 公开了一种用于婴儿和成年人急性腹膜透析的手动治疗装置，其具有最小的连接，以便最小化感染的风险，并且包括用于患者舒适和加强更有效透析的性能的加热装置。 用于婴儿和小孩的预包装设置在管道内包含用于测量用于透析的小体积的血管。 该设备能够从一个容器到四个容器处理，并且具有足够的管子长度以允许儿童或成年患者的任何量的移动。

公开(公告)号：US4319562A

公开(公告)日：1982-03-16

申请号：US61408

申请日：1979-07-27

申请人： Ivan K. Crosby

发明人： Ivan K. Crosby

IPC分类号： A61B1/07 ,  A61N1/05 ,  A61B19/00

CPC分类号： A61N1/0587 ,  A61B1/07

摘要： A method and apparatus for providing permanent epicardial pacing comparable to permanent transvenous pacing in the acutely ill or debilitated patient under local anesthesia and for drainage of pericardial fluid and pericardial biopsy. The apparatus includes a cylindrical member with first and second open end portions, an inner and outer wall portion and a connecting slot between the inner and outer wall portions, a guard member provided at the first end portion of the cylindrical member adjacent to the slot and a maneuvering member connected to the outer wall portion at the second end portion of the cylindrical member. The epicardial pacing method includes the steps of making a keyhole incision below a xyphoid process of a body, introducing a pericardioscope into a retro-sternal space of the body, making a cruciate incision in the pericardial cavity of the heart, screwing myocardial electrodes into a right ventricle of the heart, removing the pericardioscope from the retro-sternal space; attaching electrodes subcutaneously into a pocket in the left upper quadrant of the abdomen, and attaching a pulse generator to the electrodes to thereby provide epicardial pacing. The method of drainage of pericardial fluid and pericardial biopsy includes the steps of making a keyhole incision below a xyphoid process of a body, introducing a pericardioscope into a retro-sternal space of the body and excising a disc of the pericardium so as to allow for drainage of the pericardial fluid, entrance of the pericardioscope into the pericardial cavity, inspection of the epicardial surface of the heart and evaluation of the pericardium itself.

摘要翻译： 一种方法和装置，用于在局部麻醉下提供与急性或虚弱的患者的永久性静脉起搏相当的永久性心外膜起搏和用于心包液和心包活检的引流。 该装置包括：具有第一和第二开口端部的圆筒形构件，内壁部分和外壁部分之间的连接槽;保护构件，设置在与槽相邻的圆柱形构件的第一端部处， 在圆柱形构件的第二端部处连接到外壁部分的操纵构件。 心外膜起搏方法包括以下步骤：在身体的假体过程之下进行锁眼切口，将心包镜引入身体的后胸腔，在心脏的心包腔中形成十字切口，将心肌电极旋入 心脏右心室，将心包膜从后胸腔中取出; 将电极皮下附着到腹部左上腹部的口袋中，并将脉冲发生器附接到电极，从而提供心外膜起搏。 心包液和心包活检的引流方法包括以下步骤：在身体的假体过程之下进行锁眼切口，将心包镜引入身体的后胸腔并切除心包的圆盘，以便允许 心包液引流，心包膜入口进入心包腔，检查心外膜表面，并对心包本身进行评估。