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    System and method for heating, cooling and heat cycling on microfluidic device
    1.
    发明授权
    System and method for heating, cooling and heat cycling on microfluidic device 有权
    微流体装置上加热，冷却和热循环的系统和方法

    公开(公告)号：US07544506B2

    公开(公告)日：2009-06-09

    申请号：US10862826

    申请日：2004-06-07

    申请人： Wayne Breidford ,  Christy A Lancaster ,  Jon Wallace Hayenga ,  Ronald L Bardell ,  Jeffrey F Tonn ,  Bernhard H Weigl

    发明人： Wayne Breidford ,  Christy A Lancaster ,  Jon Wallace Hayenga ,  Ronald L Bardell ,  Jeffrey F Tonn ,  Bernhard H Weigl

    IPC分类号： C12M1/34 ,  C12M3/00

    CPC分类号： B01L7/52 ,  B01J2219/00873 ,  B01L3/5027 ,  B01L3/502707 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/185 ,  B01L2300/1877 ,  B01L2400/0487 ,  Y10S435/809

    摘要： An integrated heat exchange system on a microfluidic card. According to one aspect of the invention, the portable microfluidic card has a heating, cooling and heat cycling system on-board such that the card can be used portably. The microfluidic card includes one or more reservoirs containing exothermic or endothermic material. Once the chemical process of the reservoir material is activated, the reservoir provides heat or cooling to specific locations of the microfluidic card. Multiple reservoirs may be included on a single card to provide varying temperatures. The assay chemicals can be moved to the various reservoirs to create a thermal cycle useful in many biological reactions, for example, Polymerase Chain Reaction (PCR) or rtPCR. According to another aspect of the invention, the integrated heat exchanger is an adjacent microfluidic circuit containing fluid that is either independently heated or cooled, or is an exothermic or endothermic material, such that the fluid in the adjacent circuit imparts a change in temperature to the assay fluid in an independent circuit. According to yet another aspect of the invention, a thermal electric cooler (TEC) is used for thermocycling the amplification chamber of a disposable microfluidic card.

    摘要翻译： 微流控卡上集成的热交换系统。 根据本发明的一个方面，便携式微流控卡具有板上的加热，冷却和热循环系统，使得卡可以可移动地使用。 微流控卡包括一个或多个含有放热或吸热材料的储存器。 一旦储存器材料的化学过程被激活，储存器就向微流控卡的特定位置提供热量或冷却。 可以在单个卡上包括多个储存器以提供变化的温度。 测定化学品可以移动到各种储存器以产生可用于许多生物反应的热循环，例如聚合酶链式反应（PCR）或rtPCR。 根据本发明的另一方面，集成热交换器是包含独立加热或冷却的流体的相邻微流体回路，或者是放热或吸热材料，使得相邻回路中的流体将温度变化赋予 独立电路中的分析流体。 根据本发明的另一方面，热电冷却器（TEC）用于热循环一次性微流控卡的放大室。

    Functional imaging of cells with optical projection tomography
    2.
    发明授权
    Functional imaging of cells with optical projection tomography 有权
    光学投影断层扫描的细胞功能成像

    公开(公告)号：US08947510B2

    公开(公告)日：2015-02-03

    申请号：US12999515

    申请日：2009-06-08

    申请人： Michael G. Meyer ,  J. Richard Rahn ,  Anna V. Tourovskaia ,  Julia Oi Yan Yu ,  Christy A Lancaster ,  Thomas Neumann ,  Mark E. Fauver

    发明人： Michael G. Meyer ,  J. Richard Rahn ,  Anna V. Tourovskaia ,  Julia Oi Yan Yu ,  Christy A Lancaster ,  Thomas Neumann ,  Mark E. Fauver

    IPC分类号： H04N13/02 ,  G06T11/00 ,  G01N15/14

    CPC分类号： G06T11/006 ,  G01N15/1434 ,  G01N15/1475 ,  G01N21/49 ,  G01N21/6458 ,  G01N2015/1445

    摘要： A method for 3D imaging of a biologic object (1) in an optical tomography system where a subcellular structure of a biological object (1) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object (1) is moved relatively to a microscope objective (62) to present varying angles of view and the labeled biological object (1) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object (1) and the microscope objective (62) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object (1) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object (1).

    摘要翻译： 一种用于在其中通过引入至少一种纳米颗粒 - 生物标志物标记生物体（1）的亚细胞结构的光学层析成像系统中的生物物体（1）的3D成像的方法。 标记的生物体（1）相对于显微镜物镜（62）移动以呈现不同的视角，并且用波长在150nm和900nm之间的辐射照射标记的生物物体（1）。 用彩色照相机或一组至少四个单色照相机检测在至少一个波长带内通过标记的生物物体（1）和显微镜物镜（62）发射的辐射。 形成并重构来自感测辐射的生物体（1）的多个横截面图像，以便制成标记的生物体（1）的3D图像。

    System and method for heating, cooling and heat cycling on microfluidic device
    4.
    发明授权
    System and method for heating, cooling and heat cycling on microfluidic device 有权
    微流体装置上加热，冷却和热循环的系统和方法

    公开(公告)号：US07648835B2

    公开(公告)日：2010-01-19

    申请号：US12199613

    申请日：2008-08-27

    申请人： Wayne L. Breidford ,  Christy A. Lancaster ,  Jon W. Hayenga ,  Ronald L. Bardell ,  Jeffrey F. Tonn ,  Bernhard H. Weigl

    发明人： Wayne L. Breidford ,  Christy A. Lancaster ,  Jon W. Hayenga ,  Ronald L. Bardell ,  Jeffrey F. Tonn ,  Bernhard H. Weigl

    IPC分类号： C12M1/34 ,  C12M3/00

    CPC分类号： B01L3/5027 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2300/185 ,  B01L2300/1877

    摘要： An integrated heat exchange system on a microfluidic card. According to one aspect of the invention, the portable microfluidic card has a heating, cooling and heat cycling system on-board such that the card can be used portably. The microfluidic card includes one or more reservoirs containing exothermic or endothermic material. Once the chemical process of the reservoir material is activated, the reservoir provides heat or cooling to specific locations of the microfluidic card. Multiple reservoirs may be included on a single card to provide varying temperatures. The assay chemicals can be moved to the various reservoirs to create a thermal cycle useful in many biological reactions, for example, Polymerase Chain Reaction (PCR) or rtPCR. According to another aspect of the invention, the integrated heat exchanger is an adjacent microfluidic circuit containing fluid that is either independently heated or cooled, or is an exothermic or endothermic material, such that the fluid in the adjacent circuit imparts a change in temperature to the assay fluid in an independent circuit. According to yet another aspect of the invention, a thermal electric cooler (TEC) is used for thermocycling the amplification chamber of a disposable microfluidic card.

    摘要翻译： 微流控卡上集成的热交换系统。 根据本发明的一个方面，便携式微流控卡具有板上的加热，冷却和热循环系统，使得卡可以可移动地使用。 微流控卡包括一个或多个含有放热或吸热材料的储存器。 一旦储存器材料的化学过程被激活，储存器就向微流控卡的特定位置提供热量或冷却。 可以在单个卡上包括多个储存器以提供变化的温度。 测定化学品可以移动到各种储存器以产生可用于许多生物反应的热循环，例如聚合酶链式反应（PCR）或rtPCR。 根据本发明的另一方面，集成热交换器是包含独立加热或冷却的流体的相邻微流体回路，或者是放热或吸热材料，使得相邻回路中的流体将温度变化赋予 独立电路中的分析流体。 根据本发明的另一方面，热电冷却器（TEC）用于热循环一次性微流控卡的放大室。

    SYSTEM AND METHOD FOR HEATING, COOLING AND HEAT CYCLING ON MICROFLUIDIC DEVICE
    6.
    发明申请
    SYSTEM AND METHOD FOR HEATING, COOLING AND HEAT CYCLING ON MICROFLUIDIC DEVICE 有权
    用于加热，冷却和热循环的微流控装置的系统和方法

    公开(公告)号：US20090081771A1

    公开(公告)日：2009-03-26

    申请号：US12199613

    申请日：2008-08-27

    申请人： Wayne L. Breidford ,  Christy A. Lancaster ,  Jon W. Hayenga ,  Ronald L. Bardell ,  Jeffrey F. Tonn ,  Bernhard H. Weigl

    发明人： Wayne L. Breidford ,  Christy A. Lancaster ,  Jon W. Hayenga ,  Ronald L. Bardell ,  Jeffrey F. Tonn ,  Bernhard H. Weigl

    IPC分类号： C12M1/40

    CPC分类号： B01L3/5027 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2300/185 ,  B01L2300/1877

    摘要： An integrated heat exchange system on a microfluidic card. According to one aspect of the invention, the portable microfluidic card has a heating, cooling and heat cycling system on-board such that the card can be used portably. The microfluidic card includes one or more reservoirs containing exothermic or endothermic material. Once the chemical process of the reservoir material is activated, the reservoir provides heat or cooling to specific locations of the microfluidic card. Multiple reservoirs may be included on a single card to provide varying temperatures. The assay chemicals can be moved to the various reservoirs to create a thermal cycle useful in many biological reactions, for example, Polymerase Chain Reaction (PCR) or rtPCR. According to another aspect of the invention, the integrated heat exchanger is an adjacent microfluidic circuit containing fluid that is either independently heated or cooled, or is an exothermic or endothermic material, such that the fluid in the adjacent circuit imparts a change in temperature to the assay fluid in an independent circuit. According to yet another aspect of the invention, a thermal electric cooler (TEC) is used for thermocycling the amplification chamber of a disposable microfluidic card.

    摘要翻译： 微流控卡上集成的热交换系统。 根据本发明的一个方面，便携式微流控卡具有板上的加热，冷却和热循环系统，使得卡可以可移动地使用。 微流控卡包括一个或多个含有放热或吸热材料的储存器。 一旦储存器材料的化学过程被激活，储存器就向微流控卡的特定位置提供热量或冷却。 可以在单个卡上包括多个储存器以提供变化的温度。 测定化学品可以移动到各种储存器以产生可用于许多生物反应的热循环，例如聚合酶链式反应（PCR）或rtPCR。 根据本发明的另一方面，集成热交换器是包含独立加热或冷却的流体的相邻微流体回路，或者是放热或吸热材料，使得相邻回路中的流体将温度变化赋予 独立电路中的分析流体。 根据本发明的另一方面，热电冷却器（TEC）用于热循环一次性微流控卡的放大室。

    FUNCTIONAL IMAGING OF CELLS WITH OPTICAL PROJECTION TOMOGRAPHY
    7.
    发明申请
    FUNCTIONAL IMAGING OF CELLS WITH OPTICAL PROJECTION TOMOGRAPHY 有权
    用光学投影图像进行功能成像

    公开(公告)号：US20120105600A1

    公开(公告)日：2012-05-03

    申请号：US12999515

    申请日：2009-06-08

    申请人： Michael G. Meyer ,  J. Richard Rahn ,  Anna V. Tourovskaia ,  Julia Oi Yan Yu ,  Christy A. Lancaster ,  Thomas Neumann ,  Mark E. Fauver

    发明人： Michael G. Meyer ,  J. Richard Rahn ,  Anna V. Tourovskaia ,  Julia Oi Yan Yu ,  Christy A. Lancaster ,  Thomas Neumann ,  Mark E. Fauver

    IPC分类号： H04N13/02

    CPC分类号： G06T11/006 ,  G01N15/1434 ,  G01N15/1475 ,  G01N21/49 ,  G01N21/6458 ,  G01N2015/1445

    摘要： A method for 3D imaging of a biologic object (1) in an optical tomography system where a subcellular structure of a biological object (1) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object (1) is moved relatively to a microscope objective (62) to present varying angles of view and the labeled biological object (1) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object (1) and the microscope objective (62) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object (1) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object (1).

    摘要翻译： 一种用于在其中通过引入至少一种纳米颗粒 - 生物标志物标记生物体（1）的亚细胞结构的光学层析成像系统中的生物物体（1）的3D成像的方法。 标记的生物体（1）相对于显微镜物镜（62）移动以呈现不同的视角，并且用波长在150nm和900nm之间的辐射照射标记的生物物体（1）。 用彩色照相机或一组至少四个单色照相机检测在至少一个波长带内通过标记的生物物体（1）和显微镜物镜（62）发射的辐射。 形成并重构来自感测辐射的生物体（1）的多个横截面图像，以便制成标记的生物体（1）的3D图像。