公开(公告)号：US20190187155A1

公开(公告)日：2019-06-20

申请号：US16324769

申请日：2017-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel BEACHAM ,  Kyle GEE ,  Aleksey RUKAVISHNIKOV

IPC: G01N33/68 ,  C07D493/10 ,  G01N33/84

CPC classification number: G01N33/6872 ,  C07C201/00 ,  C07C229/18 ,  C07C251/30 ,  C07C2603/24 ,  C07D311/90 ,  C07D493/10 ,  C09B1/00 ,  C09B11/24 ,  G01N33/84 ,  G01N2333/705

Abstract: Compositions and methods for detecting the activity of an ion channel in a cell are described. The methods include providing a loading buffer solution to the cell, where the loading buffer includes a thallium ion indicator and optionally chloride ions, and providing a stimulus buffer that includes thallium ions to the cell. Providing the stimulus buffer can cause thallium ion influx into or efflux out of the cell through the ion channel. After providing the stimulus buffer, a change in at least one optical property of the thallium ion indicator is detected in response to thallium influx or efflux, thereby detecting the activity of the ion channel.

公开(公告)号：US20170145214A1

公开(公告)日：2017-05-25

申请号：US15423890

申请日：2017-02-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Upinder SINGH ,  Aleksey RUKAVISHNIKOV ,  Daniel BEACHAM ,  Shih-Jung HUANG ,  Michael JANES ,  Wenjun ZHOU

IPC: C09B11/24 ,  G01N33/84 ,  G01N33/58

CPC classification number: C09B11/24 ,  A61K49/0041 ,  C07D311/90 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

公开(公告)号：US20240228785A1

公开(公告)日：2024-07-11

申请号：US18419897

申请日：2024-01-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen HU ,  Aimei CHEN ,  Daniel BEACHAM ,  Chrisgen VONNEGUT ,  Krishnamurthy NACHARAJU

IPC: C09B11/24 ,  G01N21/64

CPC classification number: C09B11/24 ,  G01N21/6428 ,  G01N2021/6439

Abstract: The present disclosure provides for compounds of Formula (I),




its corresponding compounds of Formula (II)




or salts thereof and their use as fluorogenic pH sensors.

公开(公告)号：US20230129295A1

公开(公告)日：2023-04-27

申请号：US18145688

申请日：2022-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang HINZ ,  John DONOHUE ,  Daniel BEACHAM

IPC: G01N27/414 ,  C09D189/00 ,  G01N27/28 ,  G01N27/30 ,  G01N27/407

Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.

公开(公告)号：US20150104823A1

公开(公告)日：2015-04-16

申请号：US14521346

申请日：2014-10-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel BEACHAM ,  Kyle GEE

IPC: G01N33/84 ,  G01N33/68 ,  C12Q1/02

CPC classification number: G01N33/84 ,  C12Q1/02 ,  G01N33/6872 ,  G01N2333/705

Abstract: The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. The loading buffer comprises at least one thallium indicator (e.g., an environmentally sensitive, luminescent dye) and a physiological concentration of chloride ions. The methods further comprise providing a stimulus buffer to the cell, wherein the stimulus buffer comprises thallium (e.g., thallium ions). Providing the stimulus buffer causes thallium influx into the cell through the ion channel. After providing the stimulus buffer, the luminescence (e.g., fluorescence) of the dye in the cell is detected. The luminescence of the dye can change in the presence or absence of thallium. The methods may be used to measure influx or efflux of thallium through an ion channel.

Abstract translation: 本发明涉及用于检测细胞中离子通道的活性的方法。 所述方法包括向具有离子通道的电池提供加载缓冲溶液。 加载缓冲液包含至少一种铊指示剂（例如，环境敏感的发光染料）和氯离子的生理浓度。 所述方法还包括向细胞提供刺激缓冲液，其中刺激缓冲液包含铊（例如铊离子）。 提供刺激缓冲液使铊通过离子通道流入细胞。 在提供刺激缓冲液之后，检测细胞中染料的发光（例如荧光）。 在存在或不存在铊的情况下，染料的发光可以改变。 该方法可用于测量铊通过离子通道的流入或流出。

公开(公告)号：US20230120665A1

公开(公告)日：2023-04-20

申请号：US17816043

申请日：2022-07-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel BEACHAM ,  Kyle GEE

IPC: G01N33/569 ,  C12N15/09

Abstract: The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. The loading buffer comprises at least one thallium indicator (e.g., an environmentally sensitive, luminescent dye) and a physiological concentration of chloride ions. The methods further comprise providing a stimulus buffer to the cell, wherein the stimulus buffer comprises thallium (e.g., thallium ions). Providing the stimulus buffer causes thallium influx into the cell through the ion channel. After providing the stimulus buffer, the luminescence (e.g., fluorescence) of the dye in the cell is detected. The luminescence of the dye can change in the presence or absence of thallium. The methods may be used to measure influx or efflux of thallium through an ion channel.

公开(公告)号：US20160139158A1

公开(公告)日：2016-05-19

申请号：US14937736

申请日：2015-11-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey DZUBAY ,  Kyle GEE ,  Vladimir MARTIN ,  Aleksey RUKAVISHNIKOV ,  Daniel BEACHAM

IPC: G01N33/84 ,  G01N33/50 ,  G01N33/80 ,  C09B11/24 ,  G01N33/58

CPC classification number: G01N33/84 ,  C07D209/14 ,  C07D311/82 ,  C07D311/90 ,  C07D405/12 ,  C07D413/04 ,  C07D491/22 ,  C07F5/02 ,  C07K1/13 ,  C09B7/00 ,  C09B11/12 ,  C09B11/24 ,  C09B11/28 ,  C09B57/00 ,  C09B69/00 ,  C09K11/06 ,  G01N31/22 ,  G01N33/5005 ,  G01N33/5058 ,  G01N33/5091 ,  G01N33/582 ,  G01N33/80 ,  G01N2333/245 ,  Y10T436/143333

Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.

公开(公告)号：US20230324368A1

公开(公告)日：2023-10-12

申请号：US18335878

申请日：2023-06-15

Applicant: Life Technologies Corporation

Inventor： Daniel BEACHAM ,  Wolfgang HINZ ,  John DONOHUE ,  Scott PARKER

IPC: G01N33/50 ,  B01L3/00 ,  G01N15/10

CPC classification number: G01N33/5038 ,  G01N33/5044 ,  B01L3/502715 ,  G01N15/1031 ,  B01L2300/0645 ,  B01L2300/0636 ,  B01L2200/16 ,  G01N2015/0065

Abstract: Systems, devices and methods for cell analysis provide an end user with real-time cell analysis and imaging of single cells in a population. Various cell analysis systems can provide both optical imaging, as well as electroscopic imaging, which is an image of cellular response as detected by sensors covering a cell footprint or cellular efflux. An automated fluidic system can provide an end-user selected sequence of reagents to cells, while precision controlled sensor array device thermostatting, and analysis compartment environmental control provide consistency in the cell analysis system environment.

公开(公告)号：US20230279346A1

公开(公告)日：2023-09-07

申请号：US18161546

申请日：2023-01-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Soojung SHIN ,  Yiping YAN ,  Daniel BEACHAM

IPC: C12N5/0793 ,  C12N5/00 ,  C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0619 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2500/99 ,  C12N2501/42 ,  C12N2506/08

Abstract: Embodiments herein provide methods of differentiating neural stem cells to neuronal cells while concomitantly retarding neural stem cell proliferation. Resultant cultures demonstrate reduced clumping of cells, increased purity of neuronal cells and accelerated electrophysiology as compared to control methods.

公开(公告)号：US20190241869A1

公开(公告)日：2019-08-08

申请号：US16344497

申请日：2017-10-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Soojung SHIN ,  Yiping YAN ,  Daniel BEACHAM

IPC: C12N5/0793 ,  C12N5/00 ,  C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0619 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2500/99 ,  C12N2501/42 ,  C12N2506/08

Abstract: Embodiments herein provide methods of differentiating neural stem cells to neuronal cells while concomitantly retarding neural stem cell proliferation. Resultant cultures demonstrate reduced clumping of cells, increased purity of neuronal cells and accelerated electrophysiology as compared to control methods.