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公开(公告)号：US20190264274A1
公开(公告)日：2019-08-29
申请号：US16289508
申请日：2019-02-28
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , Todd Roswech , Jon A. Hoshizaki , Albert L. Carrillo , James A, Ball
IPC: C12Q1/6869 , B01F5/06 , B01F11/00 , B01L3/00 , G01N35/10 , C12Q1/6806 , B01F1/00 , G01N27/414
Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.
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公开(公告)号：US20180221865A1
公开(公告)日：2018-08-09
申请号：US15945738
申请日：2018-04-04
Applicant: Life Technologies Corporation
Inventor： Jonathan Schultz , Todd Roswech
Abstract: An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.
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公开(公告)号：US09458485B2
公开(公告)日：2016-10-04
申请号：US14799259
申请日：2015-07-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , John Nobile , Brian Reed , Prasanna Krishnan Thwar , Todd Roswech , John Andrew Sheridan
IPC: C12P19/34 , C12M1/26 , B01F15/00 , B04B11/02 , B01F5/04 , B01F13/00 , B01L3/00 , B01L7/00 , B04B5/04 , B04B7/02 , B01F3/08 , C12Q1/68
CPC classification number: B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F15/00 , B01F2215/0037 , B01L3/5021 , B01L3/502784 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/06 , B01L2300/18 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0409 , B04B5/0414 , B04B7/02 , B04B11/02 , B04B2007/025 , C12M33/10 , C12P19/34 , C12Q1/686 , C12Q2523/32 , C12Q2563/159 , C12Q2565/629
Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
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公开(公告)号：US11987842B2
公开(公告)日：2024-05-21
申请号：US17146376
申请日：2021-01-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , Todd Roswech , Jon A. Hoshizaki , Albert L. Carrillo , James A Ball
IPC: C12Q1/6869 , B01F1/00 , B01F11/00 , B01F21/20 , B01F25/451 , B01F31/31 , B01F31/65 , B01L3/00 , C12Q1/6806 , G01N27/414 , G01N35/10
CPC classification number: C12Q1/6869 , B01F21/22 , B01F25/451 , B01F31/311 , B01F31/65 , B01L3/502 , B01L3/527 , C12Q1/6806 , G01N27/4145 , G01N35/1002 , G01N35/1095 , B01L3/505 , B01L3/563 , B01L2200/04 , B01L2200/16 , B01L2300/022 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0683 , Y10T436/11 , Y10T436/143333
Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.
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公开(公告)号：US20230044160A1
公开(公告)日：2023-02-09
申请号：US17970202
申请日：2022-10-20
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , Todd Roswech
Abstract: An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.
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Patent Agency Ranking
公开(公告)号：US10934155B2
公开(公告)日：2021-03-02
申请号：US16587678
申请日：2019-09-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Todd Roswech , Jonathan Schultz , Chun Ho
Abstract: A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.
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公开(公告)号：US20170066642A1
公开(公告)日：2017-03-09
申请号：US15259245
申请日：2016-09-08
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Todd Roswech , Jonathan SCHULTZ , Chun HO
CPC classification number: B67D7/0266 , B01L3/502 , B01L3/523 , B01L3/563 , B01L2200/026 , B01L2200/0684 , B01L2200/0689 , B01L2300/043 , B01L2400/0487 , B67D7/0288 , B67D7/76 , B67D2210/0001 , G01N35/1002
Abstract: A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.
Abstract translation: 流体互连包括包括液体端口，气体端口和支架的第一接口; 第二接口，包括液体端口，气体端口和摆动杆以接合支架，容器的重量附接到第一或第二接口中的一个，以驱动第一接口的液体端口与液体端口连接 的第二接口和第一接口的气体端口连接到第二接口的气体端口。
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公开(公告)号：US10766028B2
公开(公告)日：2020-09-08
申请号：US15945738
申请日：2018-04-04
Applicant: Life Technologies Corporation
Inventor： Jonathan Schultz , Todd Roswech
Abstract: An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.
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公开(公告)号：US09890424B2
公开(公告)日：2018-02-13
申请号：US14742404
申请日：2015-06-17
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , Todd Roswech , Jon A. Hoshizaki , Albert L. Carrillo , James A. Ball
CPC classification number: C12Q1/6869 , B01F1/0027 , B01F5/0683 , B01F11/0048 , B01F11/0071 , B01L3/502 , B01L3/505 , B01L3/527 , B01L3/563 , B01L2200/04 , B01L2200/16 , B01L2300/022 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0683 , C12Q1/6806 , G01N27/4145 , G01N35/1002 , G01N35/1095 , Y10T436/11 , Y10T436/143333
Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.
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公开(公告)号：US09121047B2
公开(公告)日：2015-09-01
申请号：US13775855
申请日：2013-02-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , John Nobile , Brian Reed , Prasanna Thwar , Todd Roswech , Andrew Sheridan
IPC: C12M1/00 , C12M3/00 , C12M1/34 , C12M1/36 , C12M1/38 , C12P19/34 , C12M1/26 , B01F5/04 , B01F13/00 , B01L3/00 , B01L7/00 , B04B5/04 , B04B7/02 , C12Q1/68
CPC classification number: B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F15/00 , B01F2215/0037 , B01L3/5021 , B01L3/502784 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/06 , B01L2300/18 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0409 , B04B5/0414 , B04B7/02 , B04B11/02 , B04B2007/025 , C12M33/10 , C12P19/34 , C12Q1/686 , C12Q2523/32 , C12Q2563/159 , C12Q2565/629
Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
Abstract translation: 提供了一种自动化模板珠制备系统，包括膜基乳液生成子系统，热板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式将逆向乳液的连续流供应到热子系统。 热子系统可以处理通过其中的反相乳液。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。
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