RECOMBINANT THERAPEUTIC GLYCINE N-ACYLTRANSFERASE
    3.
    发明申请
    RECOMBINANT THERAPEUTIC GLYCINE N-ACYLTRANSFERASE 审中-公开
    重组治疗糖蛋白N-酰基转移酶

    公开(公告)号:US20170073650A1

    公开(公告)日:2017-03-16

    申请号:US15274234

    申请日:2016-09-23

    CPC classification number: C12N9/1029 C12P21/02 C12Y203/01013

    Abstract: This invention relates to a method of producing a recombinant enzyme, more particularly, this invention relates to a method of producing water soluble enzymatically active recombinant glycine N-acyltransferase (GLYAT (E.G. 2.1.3.13)), including the steps of providing a suitable expression host; preparing a vector including a gene for expressing GLYAT in the expression host to form an expression plasmid; transforming the host with the expression plasmid to form an expression system; expressing the GLYAT gene in the expression system; and separating the expressed GLYAT from the expression system.

    Abstract translation: 本发明涉及一种生产重组酶的方法,更具体地说,本发明涉及一种生产水溶性酶活性重组甘氨酸N-酰基转移酶(GLYAT(EG2.1.3.13))的方法,包括提供合适表达的步骤 主办; 制备包含用于在表达宿主中表达GLYAT的基因以形成表达质粒的载体; 用表达质粒转化宿主以形成表达系统; 在表达系统中表达GLYAT基因; 并将表达的GLYAT与表达系统分离。

    HETEROLOGOUS EXPRESSION OF GLYCINE N-ACYLTRANSFERASE PROTEINS
    8.
    发明申请
    HETEROLOGOUS EXPRESSION OF GLYCINE N-ACYLTRANSFERASE PROTEINS 审中-公开
    甘氨酸N-酰基转移酶蛋白的异位表达

    公开(公告)号:US20160090577A1

    公开(公告)日:2016-03-31

    申请号:US14865724

    申请日:2015-09-25

    CPC classification number: C12N9/1029 C12P13/02 C12P13/04 C12Y203/01013

    Abstract: The present disclosure provides novel compositions and methods for the production and use of polynucleotide sequences encoding a glycine N-acyltransferase protein (GLYAT, GLYATL 1, GLYATL 2, and GLYATL 3) for the biosynthesis of N-acylglycine biosurfactants within a heterologous expression system.

    Abstract translation: 本公开提供了用于生产和使用编码甘氨酸N-酰基转移酶蛋白(GLYAT,GLYATL 1,GLYATL 2和GLYATL 3)的多核苷酸序列用于在异源表达系统内生物合成N-酰基甘氨酸生物表面活性剂的新型组合物和方法。

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