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    Modified photosynthetic microorganisms for producing lipids
    95.
    发明授权
    Modified photosynthetic microorganisms for producing lipids 有权
    用于生产脂质的改良光合微生物

    公开(公告)号：US09523096B2

    公开(公告)日：2016-12-20

    申请号：US13995925

    申请日：2011-12-19

    申请人： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

    发明人： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

    IPC分类号： C12N1/00 ,  C12N1/21 ,  C12N15/82 ,  C07K14/195 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/90 ,  C12N9/00 ,  C12P7/64

    CPC分类号： C12N15/82 ,  C07K14/195 ,  C12N9/1029 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/90 ,  C12N9/93 ,  C12P7/64 ,  Y02P20/52

    摘要： This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that overexpress an acyl carrier protein (ACP), an acyl-ACP synthase (Aas), or both, optionally in combination with one or more overexpressed or exogenous lipid biosynthesis proteins, and/or one or more overexpressed or exogenous glycogen breakdown proteins. Exemplary biosynthesis proteins include diacyglycerol acyltransferases, thioesterases, phosphatidate phosphatases, phospholipases, triacylglycerol (TAG) hydrolases, fatty acyl-CoA synthetases, and/or acetyl-CoA carboxylases, including combinations thereof. Also included are photosynthetic microorganisms comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism. The modified photosynthetic microorganisms provided herein are capable of producing increased amounts of lipids such as fatty acids and/or synthesizing triglycerides.

    摘要翻译： 本公开描述了任选与一种或多种过表达或外源脂质生物合成蛋白组合的转基因修饰的光合微生物，例如过度表达酰基载体蛋白（ACP），酰基-ACP合酶（Aas）或两者的蓝细菌，和/ 或一种或多种过表达或外源糖原分解蛋白。 示例性生物合成蛋白包括二酰甘油酰基转移酶，硫酯酶，磷脂酸磷酸酶，磷脂酶，三酰基甘油（TAG）水解酶，脂肪酰辅酶A合成酶和/或乙酰辅酶A羧化酶，包括其组合。 还包括光合微生物，其包含糖原生物合成或储存途径中的突变或缺失，与野生型光合微生物相比，其在降低的氮条件下积累减少量的糖原。 本文提供的修饰的光合微生物能够产生增加量的脂质，例如脂肪酸和/或合成甘油三酯。

    Polypeptides with Lipase Activity And Polynucleotides Encoding Same
    99.
    发明申请
    Polypeptides with Lipase Activity And Polynucleotides Encoding Same 审中-公开
    具有脂肪酶活性的多肽和编码相同的多核苷酸

    公开(公告)号：US20160160196A1

    公开(公告)日：2016-06-09

    申请号：US14902679

    申请日：2014-07-08

    申请人： NOVOZYMES A/S

    发明人： Marc Dominique Morant ,  Anna Verena Reiser ,  Carsten Hoerslev Hansen ,  Kim Borch ,  Lone Baunsgaard ,  Vikram Kjoeller Bhatia

    IPC分类号： C12N9/20 ,  C11D3/386

    CPC分类号： C12N9/20 ,  C11D3/38627 ,  C11D3/38681 ,  C12Y301/01 ,  C12Y301/01003 ,  D06L4/40

    摘要： The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of: (a) a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 2; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 1 or the full-length complement of (i); (c)a polypeptide encoded by a polynucleotide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 1; (d) a polypeptide which is a variant of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and(e)a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d). The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.

    摘要翻译： 本发明涉及具有脂肪酶活性的分离的多肽，其选自：（a）多肽，其具有至少80％，至少85％，至少90％，至少91％，至少92％，在 与SEQ ID NO：2的至少93％，至少94％，至少95％，至少96％，至少97％，至少98％，至少99％或100％的序列同一性; （b）由多核苷酸编码的多肽，其在低严格条件，中等严格条件，中等严格条件，高严格条件或非常高严格条件下与（i）SEQ ID NO：1或全长补体 的（i）; （c）由多核苷酸编码的多肽，所述多肽具有至少80％，至少85％，至少90％，至少91％，至少92％，至少93％，至少94％，至少95％ 与SEQ ID NO：1的至少96％，至少97％，至少98％，至少99％或100％的序列同一性; （d）在一个或多个（例如多个）位置上包含取代，缺失和/或插入的SEQ ID NO：2的变体的多肽; 和（e）作为（a），（b），（c）或（d）的多肽的片段的多肽。 本发明还涉及编码多肽的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 和使用多肽的方法。

    Methods of Producing 6-Carbon Monomers From 8-Carbon Compounds
    100.
    发明申请
    Methods of Producing 6-Carbon Monomers From 8-Carbon Compounds 审中-公开
    从8碳化合物生产6碳单体的方法

    公开(公告)号：US20160152957A1

    公开(公告)日：2016-06-02

    申请号：US14947861

    申请日：2015-11-20

    申请人： INVISTA North America S.á.r.l.

    发明人： Adriana Leonora Botes ,  Alex Van Eck Conradie ,  Ramdane Haddouche

    IPC分类号： C12N9/02 ,  C12P7/42 ,  C12N9/18 ,  C12N9/20 ,  C07C47/12 ,  C07C47/19 ,  C07C49/185 ,  C07C223/02 ,  C07C229/08 ,  C12P7/64 ,  C07C229/12

    摘要： This document describes biochemical pathways for producing 6-hydroxyhexanoic acid using a monooxygenase to form a 7-hydroxyoctanoate intermediate, which can be converted to 6-hydroxyhexanoate using a polypeptide having monooxygenase, secondary alcohol dehydrogenase, or esterase activity. 6-hydroxyhexanoic acid can be enzymatically converted to adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol. This document also describes recombinant hosts producing 6-hydroxyhexanoic acid as well as adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine and 1,6-hexanediol.

    摘要翻译： 该文献描述了使用单加氧酶形成7-羟基辛酸酯中间体生产6-羟基己酸的生物化学途径，其可以使用具有单加氧酶，仲醇脱氢酶或酯酶活性的多肽转化为6-羟基己酸酯。 6-羟基己酸可以酶促转化为己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺或1,6-己二醇。 该文献还描述了生产6-羟基己酸以及己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺和1,6-己二醇的重组宿主。