中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

385 records (took 0.046 seconds)

    PROCESS OF SEPARATING COMPONENTS OF A FERMENTATION BROTH
    141.
    发明申请
    PROCESS OF SEPARATING COMPONENTS OF A FERMENTATION BROTH 有权
    分离发酵组分的方法

    公开(公告)号：US20110003355A1

    公开(公告)日：2011-01-06

    申请号：US12793623

    申请日：2010-06-03

    Applicant: Warren Clark ,  Michael Japs ,  Mark J. Burk

    Inventor： Warren Clark ,  Michael Japs ,  Mark J. Burk

    IPC: C12P7/18 ,  C07C29/76

    CPC classification number: C07C29/76 ,  C12P7/18 ,  C07C31/207

    Abstract: A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO.

    Abstract translation: 从发酵液中分离1,4-丁二醇（1,4-BDO）的方法包括从含有细胞的固体部分中分离富含1,4-BDO的液体级分，从所述液体馏分中除去水，从所述 液体馏分，并纯化1,4-BDO。 生产1,4-BDO的方法包括在发酵罐中培养产生1,4-BDO的微生物足够长的时间以产生1,4-BDO。 产生1,4-BDO的微生物包括具有1,4-BDO途径的微生物，其具有编码1,4-BDO途径酶的一种或多种外源基因和/或一种或多种基因中断。 生产1,4-BDO的方法还包括分离1,4-BDO。

    Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
    142.
    发明授权
    Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol 有权
    利用合成气或其他气态碳源和甲醇的方法和生物

    公开(公告)号：US07803589B2

    公开(公告)日：2010-09-28

    申请号：US12358217

    申请日：2009-01-22

    Applicant: Mark J. Burk ,  Christophe H. Schilling ,  Anthony P. Burgard ,  John D. Trawick

    Inventor： Mark J. Burk ,  Christophe H. Schilling ,  Anthony P. Burgard ,  John D. Trawick

    IPC: C12N9/00 ,  C12N1/20

    CPC classification number: C12P7/18 ,  C12N9/0008 ,  C12N9/0036 ,  C12N9/1007 ,  C12N9/93 ,  C12N15/70 ,  C12P19/32 ,  Y02E50/343

    Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.

    Abstract translation: 本发明提供具有乙酰-CoA途径的非天然存在的微生物生物体，以及利用合成气或合成气和甲醇的能力。 在一个实施方案中，本发明提供非天然存在的微生物，其包含一种或多种外源蛋白质，赋予微生物将CO，CO 2和/或H 2转化为乙酰辅酶A（乙酰辅酶A），甲基四氢叶酸（甲基 -THF）或其它所需产物，其中微生物缺乏在不存在一种或多种外源蛋白的情况下将CO或CO 2和H 2转化为乙酰辅酶A或甲基-THF的能力。 例如，微生物生物体可以含有至少一种编码乙酰辅酶A途径中的酶或蛋白质的外源核酸。 微生物生物能够单独或与甲醇组合使用包含CO，CO 2和/或H 2的合成气体，以产生乙酰辅酶A。 本发明另外提供了生产乙酰辅酶A的方法，例如通过培养产生乙酰辅酶A的微生物，其中微生物生物体表达至少一种编码乙酰辅酶A途径酶或蛋白质的外源核酸，其量足够 在条件下和足够的时间内产生乙酰辅酶A产生乙酰辅酶A。

    Compositions and Methods for Bioelectricity Production
    144.
    发明申请
    Compositions and Methods for Bioelectricity Production 审中-公开
    生物电生产的组成和方法

    公开(公告)号：US20080124585A1

    公开(公告)日：2008-05-29

    申请号：US11885839

    申请日：2006-03-10

    Applicant: Christophe H. Schilling

    Inventor： Christophe H. Schilling

    IPC: H01M8/16 ,  C12N1/21

    CPC classification number: H01M8/16 ,  H01M4/86 ,  H01M2004/8684 ,  Y02E60/527 ,  Y02P70/56

    Abstract: The invention provides a microbial fuel cell having a dissimilatory metal-reducing microbe expressing exogenous or native ATPase subunits, the ATPase subunits assembling into an active ATP synthase and consuming ATP in a futile cycle. The dissimilatory metal-reducing microbe can include an organism selected from the organisms set forth in Table 1. The one or more exogenous ATPase subunits can include a subunit selected from the ATPase subunits set forth in Tables 2 or 3. Also provided is a microbial fuel cell having a dissimilatory metal-reducing microbe expressing one or more exogenous genes encoding a gene product that promotes ATP consumption, the gene products of the one or more exogenous genes having an activity that reduces ATP synthesis, increases ATP consumption or both. The one or more gene products can increase ATP consumption through a futile cycle or through altering a metabolic reaction directly involved in ATP synthesis. Further provided is a microbial fuel cell having a dissimilatory metal-reducing microbe expressing one or more exogenous genes encoding a gene products that increases the electron/mole ratio compared to an unmodified microbe, wherein the increased ratio enhances electron transfer to an electrode. A method of producing electricity from an microbial organism is further provided. The method includes: (a) culturing a microbial fuel cell under anaerobic conditions sufficient for growth, the microbial fuel cell comprising a dissimilatory metal-reducing microbe expressing exogenous ATPase subunits, the ATPase subunits assembling into an active ATP synthase and consuming ATP in a futile cycle when grown under anaerobic conditions, and (b) capturing electrons produced by an increased ATP demand with an electron acceptor.

    Abstract translation: 本发明提供了一种微生物燃料电池，其具有表达外源或天然ATP酶亚基的异构金属还原微生物，ATP酶亚基组装成活性ATP合酶并在无效循环中消耗ATP。 不同的金属还原微生物可以包括选自表1所示的生物体的生物体。一种或多种外源性ATP酶亚基可以包括选自表2或3所示的ATP酶亚基的亚基。还提供了微生物燃料 具有表达编码促进ATP消耗的基因产物的一种或多种外源基因的异构金属还原微生物的细胞，具有降低ATP合成的活性的一种或多种外源基因的基因产物增加ATP消耗或两者。 一种或多种基因产物可以通过无效循环或通过改变直接参与ATP合成的代谢反应来增加ATP消耗。 还提供了具有异构金属还原微生物的微生物燃料电池，其表达一种或多种编码与未修饰微生物相比增加电子/摩尔比的基因产物的外源基因，其中增加的比例增强了电子传递到电极。 还提供了从微生物生物发电的方法。 该方法包括：（a）在足够生长的厌氧条件下培养微生物燃料电池，所述微生物燃料电池包含表达外源ATP酶亚基的异构金属还原微生物，组装成活性ATP合酶的ATP酶亚基，并消耗无效的ATP 在无氧条件下生长的循环，以及（b）用电子受体捕获由ATP需求增加产生的电子。

Patent Agency Ranking