公开(公告)号：US20140030707A1

公开(公告)日：2014-01-30

申请号：US14021260

申请日：2013-09-09

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Tan Inoue ,  Hirohide Saito ,  Yusho Kato ,  Kenichi Yoshikawa ,  Ayako Yamada ,  Toru Yamanaka

IPC: C12N15/11

CPC classification number: C12N15/11 ,  C12N15/111 ,  C12N15/67 ,  C12N2310/141 ,  C12N2320/00 ,  C12N2330/10

Abstract: An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein. The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5′ to the ribosome-binding site, and a nucleotide sequence located 5′ to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site; an mRNA comprising a small RNA-binding site located 3′ to the start codon, and a nucleotide sequence located 3′ to the small RNA-binding site, the nucleotide sequence encoding a protein; and a liposome comprising any of these mRNAs encapsulated therein.

Abstract translation: 本发明的目的是构建对短RNA序列具有特异性响应并可以活化，抑制和调节所需基因翻译的mRNA，并构建使用包含mRNA和脂质体的脂质体的人造细胞模型系统 封装在其中的无细胞翻译系统。 本发明提供：包含位于核糖体结合位点5'处的靶RNA结合位点和位于靶RNA结合位点5'的核苷酸序列的mRNA，所述核苷酸序列与核糖体结合位置互补 现场; 包含位于起始密码子3'的小RNA结合位点和位于小RNA结合位点3'的核苷酸序列的mRNA，编码蛋白质的核苷酸序列; 和包含其中包含的任何这些mRNA的脂质体。

公开(公告)号：US08586826B2

公开(公告)日：2013-11-19

申请号：US12450999

申请日：2008-04-11

Applicant: Masashi Mori ,  Koji Dohi

Inventor： Masashi Mori ,  Koji Dohi

IPC: C07K14/005 ,  C12N15/67 ,  C12N15/40

CPC classification number: C12N15/8203 ,  C12N15/8257 ,  C12N2770/00043

Abstract: It is intended to provide a polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein, and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, the linking site being located downstream of the second base sequence, the second base sequence being obtained by modifying with a base sequence in a native sequence derived from a virus by insertion, substitution, or addition. By using this, a vector containing a viral base sequence is constructed, and a protein is efficiently produced without worsening growth of a host cell containing the vector.

Abstract translation: 旨在提供包含病毒碱基序列的多核苷酸，所述病毒碱基序列包含编​​码病毒复制蛋白的第一碱基序列和编码病毒运动蛋白的第二碱基序列，所述第二碱基序列位于第一 碱基序列并具有与编码待表达多肽的外源碱基序列连接的连接位点，所述连接位点位于第二碱基序列的下游，第二碱基序列通过用天然序列衍生的碱基序列修饰获得 从病毒通过插入，替换或添加。 通过使用该方法构建含有病毒碱基序列的载体，能有效地产生蛋白质，而不会使包含载体的宿主细胞的生长恶化。

公开(公告)号：US08568470B2

公开(公告)日：2013-10-29

申请号：US13243651

申请日：2011-09-23

Applicant: Kiyohito Ishida ,  Kiyoshi Yamauchi ,  Ryosuke Kainuma ,  Yuji Sutou ,  Toshihiro Omori ,  Yuuki Tanaka ,  Hiraku Murayama ,  Ryouichi Souba

Inventor： Kiyohito Ishida ,  Kiyoshi Yamauchi ,  Ryosuke Kainuma ,  Yuji Sutou ,  Toshihiro Omori ,  Yuuki Tanaka ,  Hiraku Murayama ,  Ryouichi Souba

IPC: A61F2/82

CPC classification number: A61L31/10 ,  A61F2/91 ,  A61F2/915 ,  A61F2002/9155 ,  A61F2230/005 ,  A61F2230/0054 ,  A61F2230/008 ,  A61L31/022 ,  A61L2400/16 ,  A61M25/09 ,  A61M2025/09091 ,  A61M2025/0915

Abstract: A guide wire includes a distal core member made of a ferrous alloy which has shape memory properties and superelasticity. The ferrous alloy preferably includes substantially two phases, and has a difference of 100° C. or less between an Af point and an Ms point in a thermal hysteresis of martensitic transformation and reverse transformation. The guide wire may include a proximal core member made of an iron-containing alloy and having a higher modulus of elasticity than the distal core member. The two core members may be joined together by welding to form a core of the guide wire.

Abstract translation: 引导线包括具有形状记忆特性和超弹性的由铁合金制成的远端芯部件。 铁合金优选包括基本上两相，并且在马氏体相变和逆转变的热滞后的Af点和Ms点之间具有100℃以下的差。 引导线可以包括由含铁合金制成并且具有比远侧芯部构件更高的弹性模量的近侧芯部构件。 两个芯构件可以通过焊接连接在一起以形成导丝的芯。

公开(公告)号：US20130264540A1

公开(公告)日：2013-10-10

申请号：US13909775

申请日：2013-06-04

Applicant: Arpan Chakraborty ,  Benjamin A. Haskell ,  Stacia Keller ,  James S. Speck ,  Steven P. DenBaars ,  Shuji Nakamura ,  Umesh K. Mishra

Inventor： Arpan Chakraborty ,  Benjamin A. Haskell ,  Stacia Keller ,  James S. Speck ,  Steven P. DenBaars ,  Shuji Nakamura ,  Umesh K. Mishra

IPC: H01L33/06

CPC classification number: H01L33/06 ,  B82Y20/00 ,  C30B25/02 ,  C30B29/403 ,  H01L21/0237 ,  H01L21/02389 ,  H01L21/02433 ,  H01L21/02458 ,  H01L21/02502 ,  H01L21/0254 ,  H01L21/02609 ,  H01L33/0075 ,  H01S5/0213 ,  H01S5/3202 ,  H01S5/34333 ,  H01S2304/04 ,  H01S2304/12

Abstract: A method for the fabrication of nonpolar indium gallium nitride (InGaN) films as well as nonpolar InGaN-containing device structures using metalorganic chemical vapor deposition (MOVCD). The method is used to fabricate nonpolar InGaN/GaN violet and near-ultraviolet light emitting diodes and laser diodes.

Abstract translation: 制造非极性铟镓氮（InGaN）膜以及使用金属有机化学气相沉积（MOVCD）的非极性含InGaN的器件结构的方法。 该方法用于制造非极性InGaN / GaN紫外线和近紫外发光二极管和激光二极管。

公开(公告)号：US20130251788A1

公开(公告)日：2013-09-26

申请号：US13870002

申请日：2013-04-25

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Munetaka Kunishima

IPC: A61K9/127

CPC classification number: A61K9/1277 ,  A61K9/127 ,  B01J13/02 ,  B01J13/12 ,  Y10T428/1352 ,  Y10T428/2984 ,  Y10T428/31761

Abstract: Provided is a bilayer membrane vesicle capable of undergoing a phase transition. The bilayer membrane vesicle includes: (a) a fatty acid salt having 6 to 20 carbon atoms; (b) an alcohol or an amine compound having an aliphatic chain of 6 to 20 carbon atoms; and (c) an artificial synthetic lipid or a phospholipid capable of forming a bilayer membrane. Preferably, this bilayer membrane vesicle further contains (d) a tertiary amine as a component of the membrane. Also provided is a method of inducing a phase transition of a bilayer membrane vesicle, the method including the step of adding a dehydrating condensing agent or a dehydrating condensing agent precursor having the property of accumulating at an interface to the bilayer membrane vesicle. By causing the lipids that form a molecular aggregate to chemically change, it is possible to change the physical property and the morphology of the molecular aggregate and control the timing of phase transitions such as membrane fusion. In the membrane fusion, for example, fusion can occur without leakage of the contents of the bilayer membrane vesicle.

Abstract translation: 提供能够进行相变的双层膜囊泡。 双层膜泡囊包括：（a）具有6至20个碳原子的脂肪酸盐; （b）具有6至20个碳原子的脂族链的醇或胺化合物; 和（c）能够形成双层膜的人造合成脂质或磷脂。 优选地，该双层膜泡囊还含有（d）作为膜的组分的叔胺。 还提供了诱导双层膜囊泡相变的方法，该方法包括以下步骤：将具有在界面处积累的性质的脱水缩合剂或脱水缩合剂前体添加到双层膜泡囊。 通过使形成分子聚集体的脂质发生化学变化，可以改变分子聚集体的物理性质和形态，并且控制相变如时间的融合。 例如，在膜融合中，可以发生融合，而不会泄漏双层膜囊泡的内容物。

公开(公告)号：US20130229493A1

公开(公告)日：2013-09-05

申请号：US13823297

申请日：2011-07-27

Applicant: Koji Ikuta ,  Masashi Ikeuchi

Inventor： Koji Ikuta ,  Masashi Ikeuchi

IPC: G02B21/36

CPC classification number: G02B21/367 ,  B33Y80/00 ,  G02B21/006 ,  G02B21/0076 ,  G02B21/32 ,  G02B21/365

Abstract: Provided is a 3-dimensional confocal microscopy apparatus which is manufactured by combining a confocal microscope and an optical tweezers technique, wherein a pair of lenses for focal plane displacement where one lens is movable in the optical axis direction is arranged between a fixed objective lens and a fluorescent light imaging camera, and the 3-dimensional confocal microscopy apparatus also includes a mean which corrects the aberration of a fluorescent confocal image obtained by the fluorescent imaging camera. Accordingly, it is possible to provide a 3-dimensional confocal microscopy apparatus which can acquire a 3-dimensional image of a specimen during a manipulation of the specimen using optical tweezers without affecting an optical trap.

Abstract translation: 提供一种通过组合共聚焦显微镜和光学镊子技术制造的三维共焦显微镜装置，其中一对透镜在光轴方向上可移动的用于焦平面位移的透镜被布置在固定物镜和 荧光成像照相机和三维共聚焦显微镜装置还包括校正由荧光成像相机获得的荧光共焦图像的像差的平均值。 因此，可以提供一种三维共聚焦显微镜装置，其可以在不影响光阱的情况下使用光学镊子在使用镊子的操作期间获取样本的3维图像。

公开(公告)号：US08507220B2

公开(公告)日：2013-08-13

申请号：US10574112

申请日：2004-10-01

Applicant: Masashi Mori ,  Koji Dohi ,  Masayuki Ishikawa ,  Tetsuo Meshi ,  Masaki Nishikiori ,  Atsushi Tamai

Inventor： Masashi Mori ,  Koji Dohi ,  Masayuki Ishikawa ,  Tetsuo Meshi ,  Masaki Nishikiori ,  Atsushi Tamai

IPC: C12P21/02 ,  C12N15/04 ,  C12N15/83

CPC classification number: C12N15/8237

Abstract: An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein gene of ToMV having a suppressor against a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cells, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.

Abstract translation: 通过转移重组番茄花叶病毒（ToMV）cDNA构建表达载体，其中具有抗病毒抗性反应的抑制剂的ToMV的外壳蛋白基因已被GFP基因取代成能够诱导类固醇的启动子的下游 激素依赖性转录。 在通过将上述表达载体转移到烟草BY-2细胞获得的转化烟草BY-2细胞中，诱导类固醇激素依赖性转录，从而能够扩增GFP基因的mRNA并诱导GFP表达。

公开(公告)号：US20130143260A1

公开(公告)日：2013-06-06

申请号：US13817360

申请日：2011-08-29

Applicant: Kenichi Ogawa ,  Masanobu Nishikawa

Inventor： Kenichi Ogawa ,  Masanobu Nishikawa

IPC: C12N15/79 ,  C12P19/04

CPC classification number: C12N15/79 ,  C12N1/12 ,  C12N9/93 ,  C12N15/8245 ,  C12P19/04 ,  C12Y603/02002

Abstract: Provided are an alga in which productivity of a photosynthate is increased, and use of the alga. The alga of the present invention has an increased glutathione concentration in its chloroplast. A method of the present invention for producing biomass is a method for producing biomass with the use of the alga of the present invention or an alga produced by a method of the present invention for producing an alga.

Abstract translation: 提供了一种藻类，其中增加了光合产物的生产率和使用藻类。 本发明的藻类在其叶绿体中的谷胱甘肽浓度增加。 本发明的用于生产生物质的方法是使用本发明的藻类生产生物质的方法或通过本发明的用于生产藻类的方法生产的藻类。

公开(公告)号：US08437168B2

公开(公告)日：2013-05-07

申请号：US13607516

申请日：2012-09-07

Applicant: Sadamichi Maekawa ,  Taro Yamashita ,  Saburo Takahashi

Inventor： Sadamichi Maekawa ,  Taro Yamashita ,  Saburo Takahashi

IPC: G11C11/00

CPC classification number: G06N99/002 ,  B82Y10/00

Abstract: A Josephson quantum computing device and an integrated circuit using Josephson quantum computing devices which can realize a NOT gate operation controlled with 2 bits will be provided. The Josephson quantum computing device (1) comprises: a superconducting ring member (10) having a π-junction (6) and a 0-junction (7); and a quantum state detecting member (20) constituted by a superconducting quantum interference device arranged outside of the superconducting ring member, wherein a bonding and an antibonding state brought about by a tunneling effect between a |↑> and a |↓> state as two states degenerate in energy of the superconducting ring member (10) are regarded as quantum bits. The bonding and antibonding states as the quantum bits are read out by the quantum state detecting member (20). The two bit controlled NOT gate operation can be performed by the two quantum bits comprising said quantum bits.

公开(公告)号：US08436242B2

公开(公告)日：2013-05-07

申请号：US10592037

申请日：2005-03-09

Applicant: Shunichi Fukuzumi

Inventor： Shunichi Fukuzumi

IPC: C07D487/22 ,  B01J31/00 ,  C01B3/02 ,  H01L31/00

CPC classification number: H01L51/0078 ,  B01J31/0239 ,  B01J31/0244 ,  B01J31/1616 ,  B01J31/1815 ,  B01J35/004 ,  B01J2231/62 ,  B01J2531/025 ,  B01J2531/828 ,  C01B3/02 ,  C07D213/20 ,  C07D215/10 ,  C07D487/22 ,  Y02E10/549 ,  Y02E60/364 ,  Y02P70/521

Abstract: Long-lived charge separation is attained with high efficiency by using a supramolecular complex utilizing pi-pi interaction. A supramolecular complex is formed by pi-pi interaction between extended viologen, the extended viologen having heteroaryls coupled together by a linking group with pi-electron conjugated system, and porphyrin. As a result of study of photoelectron transfer reaction, it has been found that within the supramolecular complex, efficient electron transfer occurs from the singlet excited state of porphyrin to the extended viologen, thereby obtaining a charge separation condition of extremely long lifetime exceeding 1 millisecond.

Abstract translation: 通过使用利用pi-pi相互作用的超分子复合物，可以高效率地实现长寿命的电荷分离。 超分子复合物通过扩展的紫精，通过连接基团与π-电子共轭体系偶联在一起的杂芳基的延长紫精和卟啉形成π-π相互作用而形成。 通过研究光电子传递反应的结果，已经发现在超分子复合物中，从卟啉的单线态激发态向延伸的紫色素发生有效的电子转移，从而获得极长寿命超过1毫秒的电荷分离条件。