公开(公告)号：US07943134B2

公开(公告)日：2011-05-17

申请号：US12079576

申请日：2008-03-27

Applicant: Shie-Liang Hsieh ,  Chi-Huey Wong ,  Tsui-Ling Hsu ,  Szu-Ting Chen

Inventor： Shie-Liang Hsieh ,  Chi-Huey Wong ,  Tsui-Ling Hsu ,  Szu-Ting Chen

IPC: A61K39/395 ,  A61K39/00 ,  A61K39/193 ,  A61K45/00 ,  C12N7/00 ,  C07K16/00 ,  C12P21/08 ,  A61K39/285

CPC classification number: C07K16/2851 ,  A61K2039/505 ,  C07K2317/56 ,  C07K2317/76 ,  C07K2319/30 ,  G01N2333/18 ,  G01N2333/185 ,  G01N2500/02 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/39 ,  Y02A50/394

Abstract: Cellular receptors are identified that induce plasma leakage and other negative effects when infected with flaviviruses, such as dengue virus or Japanese encephamyelitis virus. Using fusion proteins disclosed herein, the receptors to which a pathogen, such as flavivirus, binds via glycan binding are determined. Once the receptors are determined, the effect of binding to a particular receptor may be determined, wherein targeting of the receptors causing a particular symptom may be targeted by agents that interrupt binding of the pathogen to the receptor. Accordingly, in the case of dengue virus and Japanese encephamyelitis virus, TNF-α is released when the pathogen binds to the DLVR1/CLEC5A receptor. Interrupting the DLVR1/CLEC5A receptor with monoclonal antibodies reduced TNF-α secretion without affecting secretion of cytokines responsible for viral clearance thereby increasing survival rates in infected mice from nil to around 50%.

Abstract translation: 鉴定当感染黄病毒如登革热病毒或日本脑炎病毒时诱导血浆渗漏和其它负面影响的细胞受体。 使用本文公开的融合蛋白，确定病原体（例如黄病毒）通过聚糖结合结合的受体。 一旦确定了受体，就可以确定与特定受体结合的作用，其中导致特定症状的受体的靶向可能被阻断病原体与受体结合的试剂所靶向。 因此，在登革热病毒和日本脑炎病毒的情况下，当病原体与DLVR1 / CLEC5A受体结合时，TNF-α被释放。 用单克隆抗体中断DLVR1 / CLEC5A受体降低TNF-α分泌，而不影响负责病毒清除的细胞因子分泌，从而将感染小鼠的存活率从零增加到约50％。

公开(公告)号：US07928293B2

公开(公告)日：2011-04-19

申请号：US12053792

申请日：2008-03-24

Applicant: Su-may Yu ,  Chwan-yang Hong

Inventor： Su-may Yu ,  Chwan-yang Hong

IPC: A01H1/00 ,  C07H21/04 ,  C12N15/00

CPC classification number: C12N15/8234 ,  C12N15/8257

Abstract: A nucleic acid containing a glutelin gene promoter. Disclosed are transformed plant cells and transgenic plants containing a nucleic acid that includes the promoter operably linked to a sequence encoding heterologous protein. Also disclosed are methods of making the transformed plant cells and transgenic plants and methods for expressing a polypeptide.

Abstract translation: 含有谷蛋白基因启动子的核酸。 公开了转化的植物细胞和含有核酸的转基因植物，所述核酸包括可操作地连接到编码异源蛋白质的序列的启动子。 还公开了制备转化的植物细胞和转基因植物的方法以及用于表达多肽的方法。

公开(公告)号：US07927856B2

公开(公告)日：2011-04-19

申请号：US12541322

申请日：2009-08-14

Applicant: Tuan-Hua David Ho ,  Su-May Yu ,  I Son Ng ,  Chii-Gong Tong

Inventor： Tuan-Hua David Ho ,  Su-May Yu ,  I Son Ng ,  Chii-Gong Tong

IPC: C12N9/24 ,  C07H21/04 ,  C12N15/00 ,  C12N1/20

CPC classification number: C12N9/2437 ,  C07H3/02 ,  C07H3/04 ,  C12N15/8246 ,  C12Y302/01004

Abstract: A novel thermophilic endo-glucanase, nucleic acid encoding the endo-glucase, and uses thereof in converting lignocellulosic material to fermentable sugars.

Abstract translation: 一种新型嗜热内切葡聚糖酶，编码内切葡聚糖的核酸及其在将木质纤维素材料转化成可发酵糖的用途。

公开(公告)号：US07927850B2

公开(公告)日：2011-04-19

申请号：US11490702

申请日：2006-07-21

Applicant: Jei-Fu Shaw ,  Guan-Chiun Lee ,  Yi-Shan Chen

Inventor： Jei-Fu Shaw ,  Guan-Chiun Lee ,  Yi-Shan Chen

IPC: C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC classification number: C12N9/90 ,  C12Y504/99016

Abstract: The present invention relates to the isolation and functional identification of a novel acid and heat resistant trehalose synthase enzyme cloned from Picrophilus torridus. More particularly, the present invention discloses the DNA sequence for the Picrophilus torridus trehalose synthase gene, PTTS, which when expressed in a heterologous host such as Escherichia coli, provides enzymatic activity that catalyzes the direct interconversion of maltose and trehalose through intramolecular transglycosylation. Additionally, the present invention teaches methods of use of PTTS for production of trehalose as well as for production of various useful compounds comprising trehalose.

Abstract translation: 本发明涉及从天竺葵（Trrophicus torridus）克隆的新型耐酸耐热海藻糖合成酶的分离和功能鉴定。 更具体地，本发明公开了用于在异源宿主如大肠杆菌中表达的天竺葵torridus海藻糖合酶基因PTTS的DNA序列，其提供催化通过分子内转糖基化直接相互转化麦芽糖和海藻糖的酶活性。 此外，本发明教导了使用PTTS生产海藻糖以及生产包含海藻糖的各种有用化合物的方法。

公开(公告)号：US07919521B2

公开(公告)日：2011-04-05

申请号：US12086025

申请日：2006-12-05

Applicant: Pi-Hui Liang ,  Yi-Ling Lin ,  Chi-Huey Wong

Inventor： Pi-Hui Liang ,  Yi-Ling Lin ,  Chi-Huey Wong

IPC: C07D207/00 ,  A61K31/40

CPC classification number: A61K31/4015 ,  Y02A50/385 ,  Y02A50/389

Abstract: Novel 5-membered iminocyclitol derivatives were found to be a potent and selective inhibitors of the glycoprotein processing α- and β-glucosidase which were further found to be active antiviral agents against Japanese encephalitis virus, dengue virus serotype 2 (DEN-2), human SARS coronavirus and human β-hexosaminidase, a new target for development of osteoarthritis therapeutics.

Abstract translation: 发现新的5元亚氨基环醇衍生物是糖蛋白加工α-和β-葡萄糖苷酶的有效和选择性抑制剂，其进一步被发现是针对日本脑炎病毒，登革热病毒血清型2（DEN-2）的活性抗病毒剂， 人类SARS冠状病毒和人类 - 糖尿病病毒，是骨关节炎治疗发展的新靶点。

公开(公告)号：US20110072542A1

公开(公告)日：2011-03-24

申请号：US12372231

申请日：2009-02-17

Applicant: JEI-FU SHAW ,  CHIA-PING LAI ,  PO-HONG CHEN

Inventor： JEI-FU SHAW ,  CHIA-PING LAI ,  PO-HONG CHEN

IPC: A01H5/00 ,  C12N5/10

CPC classification number: C12N15/8261 ,  C07K14/415 ,  C12N15/8271 ,  Y02A40/146

Abstract: An isolated polypeptide containing an amino acid sequence at least 70% identical to a Tubby-like protein (SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11), and an isolated nucleic acid encoding the polypeptide. Disclosed is an isolated nucleic acid that, under stringent conditions, hybridizes to a probe containing one of SEQ ID NOs:1-11; or its complementary sequence. Also disclosed are (1) a transformed cell or a transgenic plant containing such a nucleic acid and (2) a transformed cell or a transgenic plant having a reduced level(s) of one or more of the Tubby-like proteins. Also within the scope of the invention are methods for making the transformed cells or transgenic plants.

Abstract translation: 一种分离的多肽，其含有与Tubby样蛋白质（SEQ ID NO：1,2,3,4,5,6,7,8,9,10或11）至少70％相同的氨基酸序列，以及 分离的编码多肽的核酸。 公开了一种分离的核酸，其在严格条件下与含有SEQ ID NO：1-11之一的探针杂交; 或其互补序列。 还公开了（1）含有这种核酸的转化细胞或转基因植物和（2）具有降低的一种或多种类似蛋白质的水平的转化细胞或转基因植物。 在本发明的范围内也是用于制备转化细胞或转基因植物的方法。

公开(公告)号：US20110046003A1

公开(公告)日：2011-02-24

申请号：US12749118

申请日：2010-03-29

Applicant: Chi-Huey Wong ,  Chung-Yi Wu ,  Ting-Jen R. Cheng

Inventor： Chi-Huey Wong ,  Chung-Yi Wu ,  Ting-Jen R. Cheng

IPC: C40B30/04 ,  C40B40/12 ,  C40B50/00 ,  C07H9/06 ,  C07H1/00

CPC classification number: A61K39/095 ,  C07H1/00 ,  C07H3/00 ,  C07H9/06 ,  C07H11/00 ,  C07H11/04 ,  C07H13/04

Abstract: A novel N-acetyl-5-N,4-O-carbonyl-protected dibutyl sialyl phosphate donor for sialylation of both primary and sterically hindered secondary acceptors to prepare sialosides with high yield and α-selectivity is disclosed. Methods for making disaccharide building blocks comprising α(2→3), α(2→6), α(2→8), α(2→8)/α(2→9) alternate, and α(2→9) sialosides are provided. methods for one-pot synthesis of complex sialosides are disclosed. Libraries of sialosides and methods for using the libraries for detection and receptor binding analysis of surface glycoproteins or pathogens and cancer cells are disclosed. Methods for distinguishing between hemagglutinin (HA) from various strains of influenza are provided.

Abstract translation: 公开了一种新型N-乙酰基-5-N，4-O-羰基保护的二丁基唾液酸磷酸盐供体，用于主要和空间位阻二级受体的唾液酸化，以高产率和α选择性制备唾液酸苷。 包含α（2→3），α（2→6），α（2→8），α（2→8）/α（2→9）交替的二糖结构单元和α（2→9） 提供唾液酸苷。 公开了一锅合成唾液酸苷的方法。 公开了唾液酸的文库和使用文库进行表面糖蛋白或病原体和癌细胞的检测和受体结合分析的方法。 提供了用于区分来自各种流感病毒的血凝素（HA）的方法。

公开(公告)号：US20100184647A1

公开(公告)日：2010-07-22

申请号：US12685085

申请日：2010-01-11

Applicant: JYH-YIH CHEN ,  CHIA-YU PAN

Inventor： JYH-YIH CHEN ,  CHIA-YU PAN

IPC: A61K38/16 ,  A61K38/12 ,  A61P31/04

CPC classification number: A61K38/1706 ,  A61K38/1767

Abstract: Treatments of trichomonas, propionibacterium, or yeast infection using an epinecidin-1 peptide or an anti-lipopolysaccharide factor peptide.

Abstract translation: 使用表皮素-1肽或抗脂多糖因子肽治疗毛滴虫，丙酸杆菌或酵母菌感染。

公开(公告)号：US20100183649A1

公开(公告)日：2010-07-22

申请号：US12683568

申请日：2010-01-07

Applicant: Ting-Fang Wang ,  Shu-Mei Liang

Inventor： Ting-Fang Wang ,  Shu-Mei Liang

IPC: A61K39/135 ,  C12P21/00 ,  C12P21/06 ,  A61P31/12 ,  C12N1/20

CPC classification number: A61K39/135 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/552 ,  C07K2319/21 ,  C07K2319/35 ,  C07K2319/95 ,  C12N7/00 ,  C12N2770/32123 ,  C12N2770/32134

Abstract: A method for producing picornaviral capsid protein complexes (e.g., picornavirus like particles) in E. coli using a small-ubiquitin-related fusion protein expression system and an E. coli strain used in practicing this method. Also disclosed is use of the picornaviral capsid protein complexes like thus prepared for eliciting immune responses.

Abstract translation: 使用小泛素相关融合蛋白表达系统和用于实施该方法的大肠杆菌菌株在大肠杆菌中生产小核糖核酸病毒衣壳蛋白复合物（例如，小核糖核酸病毒样颗粒）的方法。 还公开了如此制备的小核糖核酸病毒衣壳蛋白复合物用于引发免疫应答。

公开(公告)号：US07737414B2

公开(公告)日：2010-06-15

申请号：US11924692

申请日：2007-10-26

Applicant: Hong-Shi Kuo ,  Ing-Shouh Hwang ,  Tien T. Tsong ,  Tsu-Yi Fu

Inventor： Hong-Shi Kuo ,  Ing-Shouh Hwang ,  Tien T. Tsong ,  Tsu-Yi Fu

IPC: H01J27/00 ,  H01J27/24 ,  H01T23/00 ,  G01Q70/10 ,  G01Q70/14 ,  G01Q70/16

CPC classification number: G01Q60/16 ,  C21D9/26 ,  C21D2201/04 ,  C22F1/14 ,  H01J1/3044 ,  H01J9/025 ,  H01J37/28 ,  H01J37/285 ,  H01J37/3056 ,  H01J2201/30411 ,  H01J2201/30449 ,  H01J2209/0226 ,  H01J2237/06 ,  H01J2237/08 ,  H01J2237/0807 ,  H01J2237/3114 ,  Y10S977/848 ,  Y10S977/90 ,  Y10S977/949 ,  Y10S977/95 ,  Y10T428/12389

Abstract: A method for preparing an iridium tip with atomic sharpness. The method includes tapering an iridium wire to a needle shape and heating the iridium needle in an oxygen atmosphere. Also disclosed is an iridium needle having a pyramidal structure which terminates with a small number of atoms prepared by the methods.

Abstract translation: 一种制备原子锐度的铱尖的方法。 所述方法包括将铱丝丝逐渐变细为针状，并在氧气氛中加热铱针。 还公开了具有锥形结构的铱针，其以通过该方法制备的少量原子终止。