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141 条记录 (耗时 0.038 秒)

    Methods of analyzing polynucleotides employing energy transfer dyes
    11.
    发明授权
    Methods of analyzing polynucleotides employing energy transfer dyes 失效
    使用能量转移染料分析多核苷酸的方法

    公开(公告)号：US07449298B2

    公开(公告)日：2008-11-11

    申请号：US11618679

    申请日：2006-12-29

    申请人： Linda G. Lee ,  Sandra L. Spurgeon ,  Barnett Rosenblum

    发明人： Linda G. Lee ,  Sandra L. Spurgeon ,  Barnett Rosenblum

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C07H21/04 ,  C07H21/00 ,  C09B11/24 ,  C12Q1/6818

    摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.

    摘要翻译： 提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。 这些连接器有助于在能量转移染料中的供体和受体染料之间的能量的有效转移。 用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基， 基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基 不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。

    Kits useful for sequencing nucleic acids
    12.
    发明授权
    Kits useful for sequencing nucleic acids 失效
    用于测序核酸的试剂盒

    公开(公告)号：US07449149B2

    公开(公告)日：2008-11-11

    申请号：US11618688

    申请日：2006-12-29

    申请人： Linda G. Lee ,  Sandra L. Spurgeon ,  Barnett Rosenblum

    发明人： Linda G. Lee ,  Sandra L. Spurgeon ,  Barnett Rosenblum

    IPC分类号： G01N15/06 ,  C12P19/34 ,  C12Q1/68 ,  C07H21/00

    CPC分类号： C07H21/04 ,  C07H21/00 ,  C09B11/24 ,  C12Q1/6818

    摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers facilitate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.

    摘要翻译： 提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。 这些接头促进能量转移染料中供体和受体染料之间的能量的有效转移。 用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基， 基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基 不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。

    Time-delay integration in a flow cytometry system
    14.
    发明授权
    Time-delay integration in a flow cytometry system 有权

    公开(公告)号：US07428047B2

    公开(公告)日：2008-09-23

    申请号：US11644412

    申请日：2006-12-22

    申请人： Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Janice G. Shigeura, legal representative

    发明人： John S. Shigeura

    IPC分类号： G01N21/00

    CPC分类号： G01N27/44721 ,  B82Y10/00 ,  B82Y20/00 ,  G01N2015/149 ,  G01N2015/1497

    摘要： An apparatus for detecting analytes in a sample is provided. The apparatus can include: a flow cytometry system including one or more detection zones; one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from detectable analytes in the one or more detection zones, which have been excited by the radiation, wherein at least one detector includes an output; a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector; and a sorting mechanism, for example, to effect flow cytometry. Methods for detecting analytes in a sample are also provided.

    Lens for capillary electrophoresis and chromatography
    19.
    发明授权
    Lens for capillary electrophoresis and chromatography 失效
    用于毛细管电泳和色谱的镜头

    公开(公告)号：US5239360A

    公开(公告)日：1993-08-24

    申请号：US561366

    申请日：1990-07-31

    申请人： Stephen E. Moring ,  Dennis E. Mead

    发明人： Stephen E. Moring ,  Dennis E. Mead

    IPC分类号： G01N21/25 ,  G01N27/447 ,  G01N30/74

    CPC分类号： G01N27/44721 ,  G01N21/255 ,  G01N2201/0638 ,  G01N30/74

    摘要： An apparatus for enhancing signal to noise ratio in the detection of electromagnetic radiation traversing a capillary tube includes a ball lens and a holding element for holding the ball lens and the capillary tube, the holding element having an aperture so that the aperature and the lens together define an optic axis. The holding element is configured to hold the capillary tube such that the center of the capillary tube traverses the optic axis. The lens has a focal length and is held at such a position relative to that focal length by the holding element such that electromagnetic radiation incident on the aperature is focussed to pass radially through the capillary tube. Thus, the effective path length is 100% of the inside diameter of the capillary tube.

    摘要翻译： 在穿过毛细管的电磁辐射的检测中提高信噪比的装置包括球透镜和用于保持球透镜和毛细管的保持元件，保持元件具有孔，使得孔和透镜在一起 定义光轴。 保持元件被配置为保持毛细管，使得毛细管的中心横穿光轴。 透镜具有焦距并通过保持元件保持在相对于该焦距的这样的位置，使得入射在温度上的电磁辐射被聚焦以径向通过毛细管。 因此，有效路径长度为毛细管内径的100％。

    Capillary electrophoresis
    20.
    发明授权
    Capillary electrophoresis 失效
    毛细管电泳

    公开(公告)号：US5207886A

    公开(公告)日：1993-05-04

    申请号：US767345

    申请日：1991-09-27

    申请人： Henk H. Lauer ,  Paul D. Grossman ,  Dennis E. Mead

    发明人： Henk H. Lauer ,  Paul D. Grossman ,  Dennis E. Mead

    IPC分类号： G01N27/447

    CPC分类号： G01N27/44756 ,  G01N27/44743

    摘要： An apparatus is disclosed for providing capillary electrophoresis, which includes an electronically controlled valve system for automatically introducing a sample into the capillary by means of a vacuum at the end of the capillary tube. This approach of sucking in the sample is extremely accurate and reproducible, and results in a minimum of band broadening. Furthermore, it enables the entire capillary electrophoresis system to be easily automated. An automated temperature control system is provided which enables the temperature of the capillary tube (and hence the solvent/solute system) to be controlled during electrophoresis, thereby very directly controlling pH and electrophoretic mobility. In another embodiment, the capillary is prewashed and equilibrated to achieve substantially zero charge on the capillary wall, thereby essentially eliminating electroosmotic flow and substantially improving resolution.

    摘要翻译： 公开了一种用于提供毛细管电泳的装置，其包括电子控制阀系统，用于通过毛细管末端的真空将样品自动引入毛细管。 吸入样品的这种方法是非常准确和可重复的，并且导致最小的带宽变宽。 此外，它使得整个毛细管电泳系统能够容易地自动化。 提供了一种自动温度控制系统，其能够在电泳期间控制毛细管（因此溶剂/溶质系统）的温度，从而非常直接地控制pH和电泳迁移率。 在另一个实施方案中，毛细管被预洗和平衡以在毛细管壁上实现基本上零的电荷，从而基本上消除电渗流并显着提高分辨率。