公开(公告)号：US20050202500A1

公开(公告)日：2005-09-15

申请号：US11118974

申请日：2005-04-28

Applicant: David Lockhart ,  Eugene Brown ,  Gordon Wong ,  Mark Chee ,  Thomas Gingeras

Inventor： David Lockhart ,  Eugene Brown ,  Gordon Wong ,  Mark Chee ,  Thomas Gingeras

IPC: G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N15/14 ,  G01N37/00 ,  C12P19/34 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC classification number: C12Q1/6809 ,  C12Q1/6837 ,  G01N15/1475 ,  C12Q2565/507

Abstract: This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.

Abstract translation: 本发明提供监测多种基因的表达水平的方法。 所述方法包括将核酸样品与寡核苷酸探针的高密度阵列杂交，其中高密度阵列含有与核酸样品中靶核酸的子序列互补的寡核苷酸探针。 在一个实施方案中，所述方法包括提供包含一个或多个靶基因的RNA转录物或衍生自RNA转录物的核酸的靶核酸库，将所述核酸库与固定在表面上的寡核苷酸探针阵列杂交，其中 包含超过100种不同寡核苷酸的阵列和每种不同的寡核苷酸定位在表面的预定区域中，不同寡核苷酸的密度大于每1cm 2大约60个不同的寡核苷酸，并且寡核苷酸 探针与衍生自RNA转录物的RNA转录物或核酸互补; 并量化阵列中的杂交核酸。

公开(公告)号：US20050158744A1

公开(公告)日：2005-07-21

申请号：US10995043

申请日：2004-11-19

Applicant: David Lockhart ,  Dirk Vetter ,  Martin Diggelmann

Inventor： David Lockhart ,  Dirk Vetter ,  Martin Diggelmann

IPC: B01J19/00 ,  C07B61/00 ,  C07H21/00 ,  C07K7/06 ,  C12N15/10 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6837 ,  C40B40/06 ,  G01N33/53 ,  G01N33/551 ,  C07H21/02 ,  A61K48/00

CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  C07B2200/11 ,  C07H21/00 ,  C07K7/06 ,  C12Q1/6811 ,  C12Q1/6837 ,  C40B40/00 ,  C40B40/06 ,  G01N33/5308 ,  G01N33/551 ,  C12Q2565/525 ,  C12Q2525/205

Abstract: Libraries of unimolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.

Abstract translation: 固体支持物上单分子双链寡核苷酸的文库。 这些文库在药物发现中可用于筛选许多生物样品，用于双链寡核苷酸与肽，蛋白质，药物和RNA之间的特异性相互作用。 在相关方面，本发明提供了在固体支持物上的构象限制探针的文库。 使用双链寡核苷酸作为脚手架，探针的运动和灵活性受到限制。 探针也可用于与药物发现和诊断相关的各种筛选程序。 本发明还提供了制备和筛选上述文库的方法。

公开(公告)号：US06705754B2

公开(公告)日：2004-03-16

申请号：US10062431

申请日：2002-01-30

Applicant: James L. Winkler ,  Mark Chee ,  David Lockhart

Inventor： James L. Winkler ,  Mark Chee ,  David Lockhart

IPC: B01F1100

CPC classification number: B01J19/28 ,  B01F9/0021 ,  B01J2219/00488 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/809

Abstract: The invention provides exemplary devices, systems and methods for mixing of fluids, such as biological fluids. One exemplary device comprises is particularly adapted for holding at least one cartridge having a chamber containing a biological fluid, with the chamber having a generally planar face. The device comprises a rotatable body having a rotational axis. The rotatable body includes at least one mounting element which is adapted to mount the rotatable body such that the face of the chamber is generally perpendicular to the rotational axis.

Abstract translation: 本发明提供了用于混合流体例如生物流体的示例性装置，系统和方法。 一个示例性装置包括特别适用于保持具有包含生物流体的腔室的至少一个筒，该腔室具有大致平坦的面。 该装置包括具有旋转轴线的可转动体。 可旋转主体包括至少一个安装元件，该安装元件适于安装可旋转主体，使得腔室的表面大致垂直于旋转轴线。

公开(公告)号：US06203989B1

公开(公告)日：2001-03-20

申请号：US09276774

申请日：1999-03-25

Applicant: Martin J. Goldberg ,  Govinda Rao S. Yelagalawadi ,  Eugene Yuji Tanimoto ,  Huu Minh Tran ,  Helin Dong ,  David Lockhart ,  Thomas B. Ryder ,  Janet A. Warrington ,  Jody Beecher

Inventor： Martin J. Goldberg ,  Govinda Rao S. Yelagalawadi ,  Eugene Yuji Tanimoto ,  Huu Minh Tran ,  Helin Dong ,  David Lockhart ,  Thomas B. Ryder ,  Janet A. Warrington ,  Jody Beecher

IPC: C12Q168

CPC classification number: C12Q1/682 ,  C12Q1/6804 ,  Y10S436/807 ,  C12Q2565/501 ,  C12Q2563/131 ,  C12Q2527/125 ,  C12Q2563/107 ,  C12Q2563/125 ,  C12Q2563/143 ,  C12Q2537/125

Abstract: Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hydridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent. Optionally, the amplification reagent, comprising a plurality of the binding ligands, is contacted with labeled receptor molecules thereby to complex a plurality of labeled receptor molecules to the amplification reagent, and the labeled receptor molecules complexed to the amplification reagent are detected. This permits the detectable signal to be enhanced and amplified. In one embodiment, the binding ligand is biotin, the receptor is streptavidin, and the amplification reagent is an antibody or a DNA matrix. In another embodiment, an array of different nucleic acid probes immobilized on a surface, each having a defined sequence and location on the surface, may be used in the assays, thus permitting screening and detection of binding of a large number of nucleic acids.

公开(公告)号：US09465935B2

公开(公告)日：2016-10-11

申请号：US13156045

申请日：2011-06-08

Applicant: Brian John Cepuran ,  Daryl McMillan ,  David Lockhart ,  Dariusz Grabka

Inventor： Brian John Cepuran ,  Daryl McMillan ,  David Lockhart ,  Dariusz Grabka

IPC: H04L29/06 ,  G06F21/51

CPC classification number: G06F21/51 ,  G06F2221/2119 ,  G06F2221/2149 ,  H04L63/101 ,  H04L63/102 ,  H04L63/145

Abstract: The invention is directed to systems, methods and apparatus for securing documents. The system comprises a server having a processor and a data storage device for storing documents, at least one document provider connected to the server, the at least one document provider operable to provide user documents to the server for storage in the data storage device, the user documents containing at least one object of security concern, and at least one document consumer connected to the server, the at least one document consumer operable to receive the user documents containing the at least one object of security concern from the server. The processor in the server is operable to determine whether to provide the at least one object of security concern to the at least one document consumer based on at least one security setting, and based on the decision either provide the documents with the at least one object of security concern or provide a replacement documents without the security of concern and an indication on each replacement document that that the at least one object of security concern has been excluded.

Abstract translation: 本发明涉及用于保护文件的系统，方法和装置。 该系统包括具有处理器和用于存储文档的数据存储设备的服务器，连接到服务器的至少一个文档提供者，所述至少一个文档提供器可操作以向服务器提供用于存储在数据存储设备中的用户文档， 包含至少一个安全关注对象的用户文档和连接到服务器的至少一个文档使用者，所述至少一个文档消费者可操作以从服务器接收包含至少一个安全关注对象的用户文档。 服务器中的处理器可操作以基于至少一个安全设置来确定是否向至少一个文档消费者提供安全关注的至少一个对象，并且基于该决定向文档提供至少一个对象 或提供替换文件，而不需要担心的安全性，以及每个替换文件的说明，表明至少有一个安全性关注对象被排除在外。

公开(公告)号：US20110307960A1

公开(公告)日：2011-12-15

申请号：US13156045

申请日：2011-06-08

Applicant: Brian John Cepuran ,  Daryl McMillan ,  David Lockhart ,  Dariusz Grabka

Inventor： Brian John Cepuran ,  Daryl McMillan ,  David Lockhart ,  Dariusz Grabka

IPC: G06F21/00

CPC classification number: G06F21/51 ,  G06F2221/2119 ,  G06F2221/2149 ,  H04L63/101 ,  H04L63/102 ,  H04L63/145

Abstract: The invention is directed to systems, methods and apparatus for securing documents. The system comprises a server having a processor and a data storage device for storing documents, at least one document provider connected to the server, the at least one document provider operable to provide user documents to the server for storage in the data storage device, the user documents containing at least one object of security concern, and at least one document consumer connected to the server, the at least one document consumer operable to receive the user documents containing the at least one object of security concern from the server. The processor in the server is operable to determine whether to provide the at least one object of security concern to the at least one document consumer based on at least one security setting, and based on the decision either provide the documents with the at least one object of security concern or provide a replacement documents without the security of concern and an indication on each replacement document that that the at least one object of security concern has been excluded.

Abstract translation: 本发明涉及用于保护文件的系统，方法和装置。 该系统包括具有处理器和用于存储文档的数据存储设备的服务器，连接到服务器的至少一个文档提供者，所述至少一个文档提供器可操作以向服务器提供用于存储在数据存储设备中的用户文档， 包含至少一个安全关注对象的用户文档和连接到服务器的至少一个文档使用者，所述至少一个文档消费者可操作以从服务器接收包含至少一个安全关注对象的用户文档。 服务器中的处理器可操作以基于至少一个安全设置来确定是否向至少一个文档消费者提供安全关注的至少一个对象，并且基于该决定向文档提供至少一个对象 或提供替换文件，而不需要担心的安全性，以及每个替换文件的说明，表明至少有一个安全性关注对象被排除在外。

公开(公告)号：US20080058219A1

公开(公告)日：2008-03-06

申请号：US11895307

申请日：2007-08-23

Applicant: David Lockhart ,  Patrick Zarrinkar ,  Daniel Treiber

Inventor： David Lockhart ,  Patrick Zarrinkar ,  Daniel Treiber

IPC: C40B30/04

CPC classification number: C12Q1/485 ,  G01N33/543 ,  G01N33/566 ,  G01N33/577 ,  G01N2333/912 ,  G01N2500/02

Abstract: The invention provides methods for determining the interactions between phage-displayed proteins and test molecules. The phage-displayed proteins are contacted with a reference moiety in the presence and absence of a test molecule; the behavior of the phage-displayed proteins as a function of concentration of the test molecule permits calculation of the binding affinity of the phage-displayed protein for the test molecule.

Abstract translation: 本发明提供了确定噬菌体展示的蛋白质和测试分子之间相互作用的方法。 在存在和不存在测试分子的情况下，将噬菌体展示的蛋白质与参考部分接触; 噬菌体展示的蛋白质作为测试分子的浓度的函数的行为允许计算噬菌体展示的蛋白质对于测试分子的结合亲和力。

公开(公告)号：US20080056994A1

公开(公告)日：2008-03-06

申请号：US11749512

申请日：2007-05-16

Applicant: Christine Kaneski ,  Sang-Hoon Shin ,  Gary Murray ,  Karin Ludwig ,  David Lockhart ,  Jeff Castelli

Inventor： Christine Kaneski ,  Sang-Hoon Shin ,  Gary Murray ,  Karin Ludwig ,  David Lockhart ,  Jeff Castelli

IPC: A61K49/00 ,  C12Q1/02

CPC classification number: A61K31/445 ,  A61K9/0053 ,  A61K9/48 ,  A61K31/45 ,  A61K45/06 ,  C07D211/46 ,  G01N33/5094 ,  G01N2333/924 ,  G01N2333/94 ,  G01N2800/04 ,  G01N2800/52 ,  A61K2300/00

Abstract: Provided are in vitro and in vivo methods for determining whether a patient with Fabry disease will respond to treatment with a specific pharmacological chaperone.

Abstract translation: 提供了用于确定法布里病患者是否将以特定药物伴侣的治疗反应的体外和体内方法。

公开(公告)号：US20080032408A1

公开(公告)日：2008-02-07

申请号：US11562548

申请日：2006-11-22

Applicant: Michael Mittmann ,  MacDonald Morris ,  Thomas B. Ryder ,  David Lockhart

Inventor： Michael Mittmann ,  MacDonald Morris ,  Thomas B. Ryder ,  David Lockhart

IPC: G01N33/00

CPC classification number: C12Q1/6837 ,  Y10T436/13 ,  C12Q2525/161

Abstract: The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses.

Abstract translation: 本发明提供了一组独特的核酸序列，其适用于需要核酸标签的各种应用。 因此，本发明的序列标签可用于例如在基因分型应用中和各种其它分析中标记生物和非生物材料。

公开(公告)号：US07157564B1

公开(公告)日：2007-01-02

申请号：US09827383

申请日：2001-04-04

Applicant: Michael Mittmann ,  MacDonald Morris ,  Thomas B. Ryder ,  David Lockhart

Inventor： Michael Mittmann ,  MacDonald Morris ,  Thomas B. Ryder ,  David Lockhart

IPC: C07H21/02

CPC classification number: C12Q1/6837 ,  Y10T436/13 ,  C12Q2525/161

Abstract: The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses.

Abstract translation: 本发明提供了一组独特的核酸序列，其适用于需要核酸标签的各种应用。 因此，本发明的序列标签可用于例如在基因分型应用中和各种其它分析中标记生物和非生物材料。